Restriction map for
pFN21AB9733
(Circular) (Six-base) MAPSORT of: pFN21AB9733.seq Check: 9861 from: 1 to: 6154
pFN21AB9733 6154 bp
With 182 enzymes: SgfI *
March 28, 2012 12:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 2298 3475 2298
Size: 1177 4977
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2684 4217 5601 5869 1632
Size: 1052 1533 1384 268 1917
Fragments arranged by size:
1917 1533 1384 1052 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 5053 6030 1203
Size: 3850 977 1327
Fragments arranged by size:
3850 1327 977
AgeI A'CCGG_T
Cuts at: 3302 4985 3302
Size: 1683 4471
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 2480 1166
Size: 1314 4840
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 6122
AlwNI CAG_nnn'CTG
Cuts at: 3897 5469 3897
Size: 1572 4582
ApaI G_GGCC'C
Cuts at: 2097 2651 2804 2823 2946 3099 3285 2097
Size: 554 153 19 123 153 186 4966
Fragments arranged by size:
4966 554 186 153 153 123 19
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 3463 3689 3841 5950 1493
Size: 1970 226 152 2109 1697
Fragments arranged by size:
2109 1970 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 2642 3092 3277 3284 3479 1870
Size: 73 681 18 450 185 7 195 4545
Fragments arranged by size:
4545 681 450 195 185 73 18 7
AvrII C'CTAG_G
Cuts at: 3115 3115
Size: 6154
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2207 2298 2314 2511
Size: 325 217 627 383 37 91 16 197
Cuts at: 2511 2893 2923 3145 3475 4930 618
Size: 382 30 222 330 1455 1842
Fragments arranged by size:
1842 1455 627 383 382 330 325 222
217 197 91 37 30 16
BanII G_rGCy'C
Cuts at: 1391 2097 2112 2132 2258 2291 2616 2651 2804
Size: 706 15 20 126 33 325 35 153
Cuts at: 2804 2823 2946 3074 3099 3285 3473 1391
Size: 19 123 128 25 186 188 4072
Fragments arranged by size:
4072 706 325 188 186 153 128 126
123 35 33 25 20 19 15
BbeI G_GCGC'C
Cuts at: 2174 2211 2318 2515 2897 3149 2174
Size: 37 107 197 382 252 5179
Fragments arranged by size:
5179 382 252 197 107 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5183
BbvCI CC'TCA_GC
Cuts at: 2744 2744
Size: 6154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3364 3398 4345 4379 1903
Size: 34 1427 34 947 34 3678
Fragments arranged by size:
3678 1427 947 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2168 4765
Size: 107 122 71 631 605 376 120 2597
Cuts at: 4765 136
Size: 1525
Fragments arranged by size:
2597 1525 631 605 376 122 120 107
71
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 2667 3571 2667
Size: 904 5250
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2097 2651 2804 2823 2946 3099 3285
Size: 276 31 554 153 19 123 153 186
Cuts at: 3285 4215 5371 1790
Size: 930 1156 2573
Fragments arranged by size:
2573 1156 930 554 276 186 153 153
123 31 19
BmgBI CAC'GTC
Cuts at: 1318 2538 1318
Size: 1220 4934
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3885 4843 442
Size: 416 53 83 399 24 2468 958 1753
Fragments arranged by size:
2468 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 3411 2788
Size: 623 5531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2612 4814 1239
Size: 944 429 2202 2579
Fragments arranged by size:
2579 2202 944 429
Bpu10I CC'TnA_GC
Cuts at: 2744 2744
Size: 6154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2501 4279 5144 5442 2372
Size: 129 1778 865 298 3084
Fragments arranged by size:
3084 1778 865 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3318 4817 882
Size: 617 1819 1499 2219
Fragments arranged by size:
2219 1819 1499 617
BsaAI yAC'GTr
Cuts at: 493 1204 2251 3945 493
Size: 711 1047 1694 2702
Fragments arranged by size:
2702 1694 1047 711
BsaBI GATnn'nnATC
Cuts at: 6148 6148
Size: 6154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2171 2208 2315 2373
Size: 53 83 186 936 638 37 107 58
Cuts at: 2373 2512 2543 2768 2894 3131 3146 3961 4343
Size: 139 31 225 126 237 15 815 382
Cuts at: 4343 4978 275
Size: 635 1451
Fragments arranged by size:
1451 936 815 638 635 382 237 225
186 139 126 107 83 58 53 37
31 15
BsaWI w'CCGG_w
Cuts at: 2352 3302 4587 4985 5259 5406 5817 2352
Size: 950 1285 398 274 147 411 2689
Fragments arranged by size:
2689 1285 950 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3236 3266 1178
Size: 30 165 30 1833 30 4066
Fragments arranged by size:
4066 1833 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 2588 2591 2676 1186
Size: 1047 355 3 85 4664
Fragments arranged by size:
4664 1047 355 85 3
BseYI C'CCAG_C
Cuts at: 2344 2831 5357 5853 2344
Size: 487 2526 496 2645
Fragments arranged by size:
2645 2526 496 487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5581
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 2223 3604 4365 5393 1540
Size: 275 186 115 107 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186 115 107
BsiHKAI G_wGCw'C
Cuts at: 2112 2409 2991 4215 4300 5371 2112
Size: 297 582 1224 85 1071 2895
Fragments arranged by size:
2895 1224 1071 582 297 85
BsiWI C'GTAC_G
Cuts at: 2247 2247
Size: 6154
BsmI GAATG_Cn'
Cuts at: 3665 3758 3665
Size: 93 6061
BsmBI CGTCTCn'nnnn_
Cuts at: 2723 2759 2723
Size: 36 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2097 2112 2132 2258 2291 2409
Size: 399 276 31 15 20 126 33 118
Cuts at: 2409 2616 2651 2804 2823 2946 2991 3074 3099
Size: 207 35 153 19 123 45 83 25
Cuts at: 3099 3285 3473 4215 4300 5371 1391
Size: 186 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 207 188 186
153 126 123 118 85 83 45 35
33 31 25 20 19 15
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 4043 5773 4043
Size: 1730 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3509 844
Size: 2665 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 3353 4041 1394
Size: 429 295 1235 688 3507
Fragments arranged by size:
3507 1235 688 429 295
BsrDI GCAATG_nn'
Cuts at: 65 1248 4649 4823 65
Size: 1183 3401 174 1396
Fragments arranged by size:
3401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2508 2813 2890 3136 3302 4798 4985
Size: 110 698 305 77 246 166 1496 187
Cuts at: 4985 1700
Size: 2869
Fragments arranged by size:
2869 1496 698 305 246 187 166 110
77
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 2504 1925
Size: 579 5575
BssSI C'ACGA_G
Cuts at: 2059 4214 5226 2059
Size: 2155 1012 2987
Fragments arranged by size:
2987 2155 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstYI r'GATC_y
Cuts at: 1972 3484 3851 4236 4253 5705 5791 5803 6149
Size: 1512 367 385 17 1452 86 12 346
Cuts at: 6149 1972
Size: 1977
Fragments arranged by size:
1977 1512 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2217 2376 2546 2913 513
Size: 552 1152 159 170 367 3754
Fragments arranged by size:
3754 1152 552 367 170 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 3176 3670 4464 4484 3176
Size: 494 794 20 4846
Fragments arranged by size:
4846 794 494 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3196 5161 809
Size: 507 1880 1965 1802
Fragments arranged by size:
1965 1880 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2220 2451
Size: 54 1425 50 275 18 283 107 231
Cuts at: 2451 3271 3601 4490 6119 8
Size: 820 330 889 1629 43
Fragments arranged by size:
1629 1425 889 820 330 283 275 231
107 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 2220 3601 1537
Size: 275 301 107 1381 4090
Fragments arranged by size:
4090 1381 301 275 107
EarI CTCTTCn'nnn_
Cuts at: 1353 2125 2565 4084 1353
Size: 772 440 1519 3423
Fragments arranged by size:
3423 1519 772 440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2029 2870 2958 4741 5115 5261
Size: 1064 138 440 841 88 1783 374 146
Cuts at: 5261 387
Size: 1280
Fragments arranged by size:
1783 1280 1064 841 440 374 146 138
88
EcoICRI GAG'CTC
Cuts at: 2110 2110
Size: 6154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2612 2684 4217 4814 5601 5869
Size: 393 551 429 72 1533 597 787 268
Cuts at: 5869 1239
Size: 1524
Fragments arranged by size:
1533 1524 787 597 551 429 393 268
72
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 3180 2477
Size: 703 5451
EcoO109I rG'GnC_Cy
Cuts at: 2093 2094 2341 2647 2648 2800 2801 2820 2860
Size: 1 247 306 1 152 1 19 40
Cuts at: 2860 2933 2943 3281 3293 2093
Size: 73 10 338 12 4954
Fragments arranged by size:
4954 338 306 247 152 73 40 19
12 10 1 1 1
EcoRI G'AATT_C
Cuts at: 3463 3841 3463
Size: 378 5776
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 2054 2174 2211 2318 2515 2891 2897 3149
Size: 376 120 37 107 197 376 6 252
Cuts at: 3149 5301 1678
Size: 2152 2531
Fragments arranged by size:
2531 2152 376 376 252 197 120 107
37 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4795 4827 4869 4901 1373
Size: 32 135 32 3223 32 42 32 2626
Fragments arranged by size:
3223 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3498 3744 669
Size: 988 281 1560 246 3079
Fragments arranged by size:
3079 1560 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KasI G'GCGC_C
Cuts at: 2170 2207 2314 2511 2893 3145 2170
Size: 37 107 197 382 252 5179
Fragments arranged by size:
5179 382 252 197 107 37
KpnI G_GTAC'C
Cuts at: 2302 3479 2302
Size: 1177 4977
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3015 3471 5268 5452 1305
Size: 1710 456 1797 184 2007
Fragments arranged by size:
2007 1797 1710 456 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 3273 10
Size: 54 1425 343 621 820 2891
Fragments arranged by size:
2891 1425 820 621 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2480 3267 3321 4112 4471 4630
Size: 648 1299 15 787 54 791 359 159
Cuts at: 4630 518
Size: 2042
Fragments arranged by size:
2042 1299 791 787 648 359 159 54
15
MspA1I CmG'CkG
Cuts at: 1606 2219 2548 2840 2915 3570 4247 5395 5640
Size: 613 329 292 75 655 677 1148 245
Cuts at: 5640 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 655 613 329 292 245
75
NaeI GCC'GGC
Cuts at: 2510 2892 3138 2510
Size: 382 246 5526
Fragments arranged by size:
5526 382 246
NarI GG'CG_CC
Cuts at: 2171 2208 2315 2512 2894 3146 2171
Size: 37 107 197 382 252 5179
Fragments arranged by size:
5179 382 252 197 107 37
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5602
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NgoMIV G'CCGG_C
Cuts at: 2508 2890 3136 2508
Size: 382 246 5526
Fragments arranged by size:
5526 382 246
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 2220 3601 2220
Size: 1381 4773
NspI r_CATG'y
Cuts at: 1132 1229 3512 5057 1132
Size: 97 2283 1545 2229
Fragments arranged by size:
2283 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2320 1348
Size: 972 5182
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3006 1108
Size: 1898 4256
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3236 3268 4223 4255 5736 5768 3056
Size: 32 148 32 955 32 1481 32 3442
Fragments arranged by size:
3442 1481 955 148 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2860 2933 2341
Size: 519 73 5562
Fragments arranged by size:
5562 519 73
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PspOMI G'GGCC_C
Cuts at: 2093 2647 2800 2819 2942 3095 3281 2093
Size: 554 153 19 123 153 186 4966
Fragments arranged by size:
4966 554 186 153 153 123 19
PstI C_TGCA'G
Cuts at: 830 2722 2799 3506 830
Size: 1892 77 707 3478
Fragments arranged by size:
3478 1892 707 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
RsrII CG'GwC_CG
Cuts at: 3305 3305
Size: 6154
SacI G_AGCT'C
Cuts at: 2112 2112
Size: 6154
SacII CC_GC'GG
Cuts at: 2220 2549 2916 2220
Size: 329 367 5458
Fragments arranged by size:
5458 367 329
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SanDI GG'GwC_CC
Cuts at: 2341 2933 2341
Size: 592 5562
SapI GCTCTTCn'nnn_
Cuts at: 2125 2125
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 2722 3506 2722
Size: 784 5370
ScaI AGT'ACT
Cuts at: 4402 6114 4402
Size: 1712 4442
SexAI A'CCwGG_T
Cuts at: 2489 2489
Size: 6154
SfcI C'TryA_G
Cuts at: 826 1045 2718 2795 3502 4637 5318 5509 826
Size: 219 1673 77 707 1135 681 191 1471
Fragments arranged by size:
1673 1471 1135 707 681 219 191 77
SfoI GGC'GCC
Cuts at: 2172 2209 2316 2513 2895 3147 2172
Size: 37 107 197 382 252 5179
Fragments arranged by size:
5179 382 252 197 107 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 2508 2813 2890 1700
Size: 808 305 77 4964
Fragments arranged by size:
4964 808 305 77
SmaI CCC'GGG
Cuts at: 1872 3094 3279 3286 3481 1872
Size: 1222 185 7 195 4545
Fragments arranged by size:
4545 1222 195 185 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 2516 4258 5159 5421 820
Size: 197 926 444 129 1742 901 262 1553
Fragments arranged by size:
1742 1553 926 901 444 262 197 129
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SrfI GCCC'GGGC
Cuts at: 3286 3286
Size: 6154
SspI AAT'ATT
Cuts at: 5 52 4078 5
Size: 47 4026 2081
Fragments arranged by size:
4026 2081 47
StuI AGG'CCT
Cuts at: 1517 2393 1517
Size: 876 5278
StyI C'CwwG_G
Cuts at: 513 1065 2292 2430 3115 3593 513
Size: 552 1227 138 685 478 3074
Fragments arranged by size:
3074 1227 685 552 478 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2492 2725 4181 4198 4351 4536 4981
Size: 1255 912 233 1456 17 153 185 445
Cuts at: 4981 325
Size: 1498
Fragments arranged by size:
1498 1456 1255 912 445 233 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4400 6112 96
Size: 274 80 33 51 1443 2423 1712 138
Fragments arranged by size:
2423 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3289 3321 6114 6146 3289
Size: 32 2793 32 3297
Fragments arranged by size:
3297 2793 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3041 1316
Size: 1725 4429
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3092 3277 3284 3479 1870
Size: 1222 185 7 195 4545
Fragments arranged by size:
4545 1222 195 185 7
XmnI GAAnn'nnTTC
Cuts at: 2697 4283 2697
Size: 1586 4568
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BstXI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII SfiI SwaI XcmI