Restriction map for
pFN21AB9730
(Circular) (Six-base) MAPSORT of: pFN21AB9730.seq Check: 7196 from: 1 to: 6100
pFN21AB9730 6100 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2145 2145
Size: 6100
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3443 1937
Size: 1506 4594
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4163 5547 5815 1632
Size: 2531 1384 268 1917
Fragments arranged by size:
2531 1917 1384 268
AfeI AGC'GCT
Cuts at: 2924 2924
Size: 6100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2153 1184
Size: 969 5131
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 6068
AlwNI CAG_nnn'CTG
Cuts at: 2338 2425 3268 3843 5415 2338
Size: 87 843 575 1572 3023
Fragments arranged by size:
3023 1572 843 575 87
ApaI G_GGCC'C
Cuts at: 2227 2227
Size: 6100
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3409 3635 3787 5896 1493
Size: 1916 226 152 2109 1697
Fragments arranged by size:
2109 1916 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2611 2808 3425 1870
Size: 73 668 197 617 4545
Fragments arranged by size:
4545 668 617 197 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2728 3421 4876 618
Size: 325 217 627 278 663 693 1455 1842
Fragments arranged by size:
1842 1455 693 663 627 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2227 2595 2817 2919 3419 1391
Size: 836 368 222 102 500 4072
Fragments arranged by size:
4072 836 500 368 222 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3169 928
Size: 971 1270 3859
Fragments arranged by size:
3859 1270 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4291 4325 1903
Size: 34 2354 34 3678
Fragments arranged by size:
3678 2354 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2244 2430 4711
Size: 107 122 71 631 605 572 186 2281
Cuts at: 4711 136
Size: 1525
Fragments arranged by size:
2281 1525 631 605 572 186 122 107
71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 3103 3281 3517 3103
Size: 178 236 5686
Fragments arranged by size:
5686 236 178
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2227 2733 2804 4161 5317 1790
Size: 280 157 506 71 1357 1156 2573
Fragments arranged by size:
2573 1357 1156 506 280 157 71
BmgBI CAC'GTC
Cuts at: 1318 2518 1318
Size: 1200 4900
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3831 4789 442
Size: 416 53 83 399 24 2414 958 1753
Fragments arranged by size:
2414 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3367 3367
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2536 3034 4760 1239
Size: 1297 498 1726 2579
Fragments arranged by size:
2579 1726 1297 498
Bpu10I CC'TnA_GC
Cuts at: 2229 2229
Size: 6100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2903 4225 5090 5388 2903
Size: 1322 865 298 3615
Fragments arranged by size:
3615 1322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3032 4763 882
Size: 617 1533 1731 2219
Fragments arranged by size:
2219 1731 1533 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2171 3907 4289 4924
Size: 53 83 186 936 638 1736 382 635
Cuts at: 4924 275
Size: 1451
Fragments arranged by size:
1736 1451 936 638 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2558 3045 4533 4931 5205 5352 5763 2558
Size: 487 1488 398 274 147 411 2895
Fragments arranged by size:
2895 1488 487 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 3062 3092 1178
Size: 30 165 30 738 30 891 30 4186
Fragments arranged by size:
4186 891 738 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3334 1186
Size: 2148 3952
BseYI C'CCAG_C
Cuts at: 2086 2583 3261 3268 5303 5799 2086
Size: 497 678 7 2035 496 2387
Fragments arranged by size:
2387 2035 678 497 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2027 1163
Size: 573 291 5236
Fragments arranged by size:
5236 573 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2686 2721 3034 3550 4311
Size: 275 186 33 652 35 313 516 761
Cuts at: 4311 5339 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 652 516 313 275 186
35 33
BsiHKAI G_wGCw'C
Cuts at: 2235 2919 2964 3287 4161 4246 5317 2235
Size: 684 45 323 874 85 1071 3018
Fragments arranged by size:
3018 1071 874 684 323 85 45
BsmI GAATG_Cn'
Cuts at: 3611 3704 3611
Size: 93 6007
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2227 2235 2595 2733 2804 2817
Size: 399 280 157 8 360 138 71 13
Cuts at: 2817 2919 2964 3287 3419 4161 4246 5317 1391
Size: 102 45 323 132 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 323 280 157
138 132 102 85 71 45 13 8
BspEI T'CCGG_A
Cuts at: 3045 5763 3045
Size: 2718 3382
BspHI T'CATG_A
Cuts at: 3303 3989 5719 3303
Size: 686 1730 3684
Fragments arranged by size:
3684 1730 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2091 2145 2830 3000 3455 844
Size: 1247 54 685 170 455 3489
Fragments arranged by size:
3489 1247 685 455 170 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2442 2970 3987 1394
Size: 429 619 528 1017 3507
Fragments arranged by size:
3507 1017 619 528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2854 4595 4769 65
Size: 1183 1606 1741 174 1396
Fragments arranged by size:
1741 1606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2243 2522 2720 2831 4744 4931
Size: 110 252 181 279 198 111 1913 187
Cuts at: 4931 1700
Size: 2869
Fragments arranged by size:
2869 1913 279 252 198 187 181 111
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 2105 2835 1925
Size: 180 730 5190
Fragments arranged by size:
5190 730 180
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2338 2959 794
Size: 1544 621 3935
Fragments arranged by size:
3935 1544 621
BstBI TT'CG_AA
Cuts at: 2658 4904 2658
Size: 2246 3854
BstEII G'GTnAC_C
Cuts at: 1184 2827 1184
Size: 1643 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 2167 2269 2893 2167
Size: 102 624 5374
Fragments arranged by size:
5374 624 102
BstYI r'GATC_y
Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095
Size: 1458 367 385 17 1452 86 12 346
Cuts at: 6095 1972
Size: 1977
Fragments arranged by size:
1977 1458 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2115 2125 2160 2268 2689 2785
Size: 552 957 93 10 35 108 421 96
Cuts at: 2785 513
Size: 3828
Fragments arranged by size:
3828 957 552 421 108 96 93 35
10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 3616 4410 4430 3616
Size: 794 20 5286
Fragments arranged by size:
5286 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DraIII CAC_nnn'GTG
Cuts at: 2050 2050
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2520 2673
Size: 54 1425 50 275 18 411 279 153
Cuts at: 2673 2683 2718 3031 3547 4436 6065 8
Size: 10 35 313 516 889 1629 43
Fragments arranged by size:
1629 1425 889 516 411 313 279 275
153 54 50 43 35 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2683 2718 3031 3547 1537
Size: 275 871 35 313 516 4090
Fragments arranged by size:
4090 871 516 313 275 35
EarI CTCTTCn'nnn_
Cuts at: 1353 2868 4030 1353
Size: 1515 1162 3423
Fragments arranged by size:
3423 1515 1162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2214 2274 4687 5061 5207 387
Size: 1064 138 625 60 2413 374 146 1280
Fragments arranged by size:
2413 1280 1064 625 374 146 138 60
EcoICRI GAG'CTC
Cuts at: 2917 2917
Size: 6100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2536 3034 4163 4760 5547 5815 1239
Size: 393 904 498 1129 597 787 268 1524
Fragments arranged by size:
1524 1129 904 787 597 498 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3046 3046
Size: 6100
EcoO109I rG'GnC_Cy
Cuts at: 2224 2979 2224
Size: 755 5345
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FseI GG_CCGG'CC
Cuts at: 2247 2247
Size: 6100
FspI TGC'GCA
Cuts at: 2999 4606 2999
Size: 1607 4493
HaeII r_GCGC'y
Cuts at: 1678 2425 2926 5247 1678
Size: 747 501 2321 2531
Fragments arranged by size:
2531 2321 747 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4741 4773 4815 4847 1373
Size: 32 135 32 3169 32 42 32 2626
Fragments arranged by size:
3169 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3690 669
Size: 988 281 1506 246 3079
Fragments arranged by size:
3079 1506 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3255 5214 5398 1305
Size: 1950 1959 184 2007
Fragments arranged by size:
2007 1959 1950 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4278
Fragments arranged by size:
4278 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2267 2456 4058 4417 4576 518
Size: 648 999 102 189 1602 359 159 2042
Fragments arranged by size:
2042 1602 999 648 359 189 159 102
MspA1I CmG'CkG
Cuts at: 1606 2017 2127 2193 2338 3268 3516 4193 5341
Size: 411 110 66 145 930 248 677 1148
Cuts at: 5341 5586 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 930 677 411 248 245 145
110 66
NaeI GCC'GGC
Cuts at: 2245 2524 2722 2245
Size: 279 198 5623
Fragments arranged by size:
5623 279 198
NcoI C'CATG_G
Cuts at: 513 1065 2022 2160 2268 513
Size: 552 957 138 108 4345
Fragments arranged by size:
4345 957 552 138 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NgoMIV G'CCGG_C
Cuts at: 2243 2522 2720 2243
Size: 279 198 5623
Fragments arranged by size:
5623 279 198
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2302 3091 3114 3209 3458 5003 1132
Size: 97 1073 789 23 95 249 1545 2229
Fragments arranged by size:
2229 1545 1073 789 249 97 95 23
PasI CC'CwG_GG
Cuts at: 1348 2779 2974 1348
Size: 1431 195 4474
Fragments arranged by size:
4474 1431 195
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 3012 3268 1108
Size: 1317 587 256 3940
Fragments arranged by size:
3940 1317 587 256
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 4169 4201 5682 5714 3062
Size: 32 1075 32 1481 32 3448
Fragments arranged by size:
3448 1481 1075 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2979 2979
Size: 6100
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspOMI G'GGCC_C
Cuts at: 2223 2223
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2334 3069 3452 830
Size: 1504 735 383 3478
Fragments arranged by size:
3478 1504 735 383
PvuI CG_AT'CG
Cuts at: 2001 2034 2001
Size: 33 6067
PvuII CAG'CTG
Cuts at: 2017 3268 2017
Size: 1251 4849
SacI G_AGCT'C
Cuts at: 2919 2919
Size: 6100
SacII CC_GC'GG
Cuts at: 2128 2128
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SanDI GG'GwC_CC
Cuts at: 2979 2979
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2330 2926 3065 3448 4583 5264 5455
Size: 219 1285 596 139 383 1135 681 191
Cuts at: 5455 826
Size: 1471
Fragments arranged by size:
1471 1285 1135 681 596 383 219 191
139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 2613 2810 3427 1872
Size: 741 197 617 4545
Fragments arranged by size:
4545 741 617 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2918 4204 5105 5367 820
Size: 197 926 975 1286 901 262 1553
Fragments arranged by size:
1553 1286 975 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 2302 3114 3458 1132
Size: 1170 812 344 3774
Fragments arranged by size:
3774 1170 812 344
SspI AAT'ATT
Cuts at: 5 52 3213 4024 5
Size: 47 3161 811 2081
Fragments arranged by size:
3161 2081 811 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2022 2160 2268 3539 513
Size: 552 957 138 108 1271 3074
Fragments arranged by size:
3074 1271 957 552 138 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3172 4127 4144 4297 4482 4927 325
Size: 1255 1592 955 17 153 185 445 1498
Fragments arranged by size:
1592 1498 1255 955 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4346 6058 96
Size: 274 80 33 51 1443 2369 1712 138
Fragments arranged by size:
2369 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 6060 6092 2141
Size: 32 3887 32 2149
Fragments arranged by size:
3887 2149 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2560 2266
Size: 294 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 2611 2808 3425 1870
Size: 741 197 617 4545
Fragments arranged by size:
4545 741 617 197
XmnI GAAnn'nnTTC
Cuts at: 2857 4229 2857
Size: 1372 4728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FalI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI