Restriction map for
pFN21AB9724
(Circular) (Six-base) MAPSORT of: pFN21AB9724.seq Check: 9714 from: 1 to: 5959
pFN21AB9724 5959 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2707 2707
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AfeI AGC'GCT
Cuts at: 2822 3104 2822
Size: 282 5677
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2618 2650 2618
Size: 32 5927
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaLI G'TGCA_C
Cuts at: 2272 4016 5172 2272
Size: 1744 1156 3059
Fragments arranged by size:
3059 1744 1156
ApoI r'AATT_y
Cuts at: 1493 3242 3268 3494 3646 5755 1493
Size: 1749 26 226 152 2109 1697
Fragments arranged by size:
2109 1749 1697 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2440 2458 2476 2489 2512 2533 2651
Size: 73 497 18 18 13 23 21 118
Cuts at: 2651 2804 2896 2999 3095 3110 3284 1870
Size: 153 92 103 96 15 174 4545
Fragments arranged by size:
4545 497 174 153 118 103 96 92
73 23 21 18 18 15 13
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2290 2426 3014 3280 4735
Size: 325 217 627 503 136 588 266 1455
Cuts at: 4735 618
Size: 1842
Fragments arranged by size:
1842 1455 627 588 503 325 266 217
136
BanII G_rGCy'C
Cuts at: 1391 2547 2805 2873 2909 3048 3278 1391
Size: 1156 258 68 36 139 230 4072
Fragments arranged by size:
4072 1156 258 230 139 68 36
BbeI G_GCGC'C
Cuts at: 2430 3018 2430
Size: 588 5371
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2125 2159 2712 2746 4150 4184 1903
Size: 34 188 34 553 34 1404 34 3678
Fragments arranged by size:
3678 1404 553 188 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BfrBI ATG'CAT
Cuts at: 2044 2044
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2445 2463 2481
Size: 107 122 71 631 605 773 18 18
Cuts at: 2481 2517 2538 2638 4570 136
Size: 36 21 100 1932 1525
Fragments arranged by size:
1932 1525 773 631 605 122 107 100
71 36 21 18 18
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2276 4020 5176 1790
Size: 486 1744 1156 2573
Fragments arranged by size:
2573 1744 1156 486
BmgBI CAC'GTC
Cuts at: 1318 2277 2866 1318
Size: 959 589 4411
Fragments arranged by size:
4411 959 589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 4619 1239
Size: 829 2551 2579
Fragments arranged by size:
2579 2551 829
Bpu10I CC'TnA_GC
Cuts at: 2058 3042 2058
Size: 984 4975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2873 2960 4084 4949 5247 2873
Size: 87 1124 865 298 3585
Fragments arranged by size:
3585 1124 865 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2247 3750 493
Size: 711 1043 1503 2702
Fragments arranged by size:
2702 1503 1043 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 2427 2768 3015
Size: 53 83 186 936 708 186 341 247
Cuts at: 3015 3766 4148 4783 275
Size: 751 382 635 1451
Fragments arranged by size:
1451 936 751 708 635 382 341 247
186 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2072 2102 2345 2375 1178
Size: 30 165 30 669 30 243 30 4762
Fragments arranged by size:
4762 669 243 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2353 3017 1186
Size: 1167 664 4128
Fragments arranged by size:
4128 1167 664
BseYI C'CCAG_C
Cuts at: 2845 3018 5162 5658 2845
Size: 173 2144 496 3146
Fragments arranged by size:
3146 2144 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 3149 1163
Size: 573 327 1086 3973
Fragments arranged by size:
3973 1086 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 2415 2619 2712 2745 3409
Size: 275 186 192 222 204 93 33 664
Cuts at: 3409 4170 5198 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 275 222 204 192
186 93 33
BsiHKAI G_wGCw'C
Cuts at: 2064 2276 2805 2924 3048 4020 4105 5176 2064
Size: 212 529 119 124 972 85 1071 2847
Fragments arranged by size:
2847 1071 972 529 212 124 119 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
BsmBI CGTCTCn'nnnn_
Cuts at: 2153 2153
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2276 2547 2805 2873 2909 2924
Size: 399 274 212 271 258 68 36 15
Cuts at: 2924 3048 3278 4020 4105 5176 1391
Size: 124 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 274 271 258 230
212 124 85 68 36 15
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2707 2922 3314 844
Size: 1863 215 392 3489
Fragments arranged by size:
3489 1863 392 215
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2387 2414 4603 4790 1700
Size: 110 577 27 2189 187 2869
Fragments arranged by size:
2869 2189 577 187 110 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2928 794
Size: 2134 3825
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2892 3289 3656 4041 4058 5510 5596 5608
Size: 920 397 367 385 17 1452 86 12
Cuts at: 5608 5954 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 920 397 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2193 2286 2607 2677 2745 513
Size: 552 1128 93 321 70 68 3727
Fragments arranged by size:
3727 1128 552 321 93 70 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2185 3070 3148 487
Size: 683 56 959 885 78 3298
Fragments arranged by size:
3298 959 885 683 78 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2385 2412
Size: 54 1425 50 275 18 360 195 27
Cuts at: 2412 2616 2709 2742 2880 3406 4295 5924 8
Size: 204 93 33 138 526 889 1629 43
Fragments arranged by size:
1629 1425 889 526 360 275 204 195
138 93 54 50 43 33 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2190 2412 2616 2709 2742 3406 1537
Size: 275 378 222 204 93 33 664 4090
Fragments arranged by size:
4090 664 378 275 222 204 93 33
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 3053 3889 1353
Size: 794 906 836 3423
Fragments arranged by size:
3423 906 836 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2803 3046 2803
Size: 243 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 4022 4619 5406 5674 1239
Size: 393 436 1954 597 787 268 1524
Fragments arranged by size:
1954 1524 787 597 436 393 268
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2186 2333 2430 2824 2879 3018 3106 5106
Size: 508 147 97 394 55 139 88 2000
Cuts at: 5106 1678
Size: 2531
Fragments arranged by size:
2531 2000 508 394 147 139 97 88
55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2855 2887 4600
Size: 32 135 32 773 32 478 32 1713
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1713 773 478 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2426 3014 2426
Size: 588 5371
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 10
Size: 54 1425 343 1050 3087
Fragments arranged by size:
3087 1425 1050 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2627 3917 4276 4435 518
Size: 648 1038 423 1290 359 159 2042
Fragments arranged by size:
2042 1290 1038 648 423 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2609 2690 2696 2726 2759 2833 3375
Size: 522 481 81 6 30 33 74 542
Cuts at: 3375 4052 5200 5445 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 542 522 481 245 81
74 33 30 6
NaeI GCC'GGC
Cuts at: 2389 2416 2389
Size: 27 5932
NarI GG'CG_CC
Cuts at: 2427 3015 2427
Size: 588 5371
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 2577 387
Size: 2190 3769
NgoMIV G'CCGG_C
Cuts at: 2387 2414 2387
Size: 27 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 2616 3406 2616
Size: 790 5169
NsiI A_TGCA'T
Cuts at: 2046 2046
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2632 3317 4862 1132
Size: 97 1403 685 1545 2229
Fragments arranged by size:
2229 1545 1403 685 97
PasI CC'CwG_GG
Cuts at: 1348 2212 1348
Size: 864 5095
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspXI vC'TCGA_Gb
Cuts at: 2804 2999 3110 2804
Size: 195 111 5653
Fragments arranged by size:
5653 195 111
PstI C_TGCA'G
Cuts at: 830 2133 2933 2991 3311 830
Size: 1303 800 58 320 3478
Fragments arranged by size:
3478 1303 800 320 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacI G_AGCT'C
Cuts at: 2805 3048 2805
Size: 243 5716
SacII CC_GC'GG
Cuts at: 2610 2610
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 3053 3053
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 2991 3311 2991
Size: 320 5639
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2129 2929 2987 3307 4442 5123 5314
Size: 219 1084 800 58 320 1135 681 191
Cuts at: 5314 826
Size: 1471
Fragments arranged by size:
1471 1135 1084 800 681 320 219 191
58
SfoI GGC'GCC
Cuts at: 2428 3016 2428
Size: 588 5371
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 2442 2460 2478 2491 2514 2535 2653 2898
Size: 570 18 18 13 23 21 118 245
Cuts at: 2898 3286 1872
Size: 388 4545
Fragments arranged by size:
4545 570 388 245 118 23 21 18
18 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2804 2888 2975 2999 3110 4063
Size: 197 926 861 84 87 24 111 953
Cuts at: 4063 4964 5226 820
Size: 901 262 1553
Fragments arranged by size:
1553 953 926 901 861 262 197 111
87 84 24
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SrfI GCCC'GGGC
Cuts at: 2460 2478 2535 2460
Size: 18 57 5884
Fragments arranged by size:
5884 57 18
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 3116 1517
Size: 1599 4360
StyI C'CwwG_G
Cuts at: 513 1065 3398 513
Size: 552 2333 3074
Fragments arranged by size:
3074 2333 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 4003 4156 4341 4786 325
Size: 1255 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 5919 5951 2072
Size: 32 3815 32 2080
Fragments arranged by size:
3815 2080 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2293 2293
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 2804 2999 3110 1943
Size: 861 195 111 4792
Fragments arranged by size:
4792 861 195 111
XmaI C'CCGG_G
Cuts at: 1870 2440 2458 2476 2489 2512 2533 2651 2896
Size: 570 18 18 13 23 21 118 245
Cuts at: 2896 3284 1870
Size: 388 4545
Fragments arranged by size:
4545 570 388 245 118 23 21 18
18 13
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI BsiWI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
NruI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII
RsrII SanDI SexAI SfiI SwaI