Restriction map for
pFN21AB9723
(Circular) (Six-base) MAPSORT of: pFN21AB9723.seq Check: 8196 from: 1 to: 5947
pFN21AB9723 5947 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2425 2425
Size: 5947
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2468 4010 5394 5662 1632
Size: 836 1542 1384 268 1917
Fragments arranged by size:
1917 1542 1384 836 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2673 2886 4846 5823 1203
Size: 1470 213 1960 977 1327
Fragments arranged by size:
1960 1470 1327 977 213
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3043 1184
Size: 1859 4088
AlwNI CAG_nnn'CTG
Cuts at: 2629 3103 3690 5262 2629
Size: 474 587 1572 3314
Fragments arranged by size:
3314 1572 587 474
ApaI G_GGCC'C
Cuts at: 2090 2510 2993 2090
Size: 420 483 5044
Fragments arranged by size:
5044 483 420
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 3089 3256 3482 3634 5743 1493
Size: 1596 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 1596 226 167 152
AscI GG'CGCG_CC
Cuts at: 2634 2634
Size: 5947
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 2510 2555 2729 2912 2986 3068 3272
Size: 73 567 45 174 183 74 82 204
Cuts at: 3272 1870
Size: 4545
Fragments arranged by size:
4545 567 204 183 174 82 74 73
45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2017 2050 2017
Size: 33 5914
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2750 2866 3268 4723
Size: 325 217 627 359 604 116 402 1455
Cuts at: 4723 618
Size: 1842
Fragments arranged by size:
1842 1455 627 604 402 359 325 217
116
BanII G_rGCy'C
Cuts at: 1391 2090 2117 2510 2838 2909 2993 3066 3266
Size: 699 27 393 328 71 84 73 200
Cuts at: 3266 1391
Size: 4072
Fragments arranged by size:
4072 699 393 328 200 84 73 71
27
BbeI G_GCGC'C
Cuts at: 2150 2870 2150
Size: 720 5227
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2018 2216 3839 5049 2018
Size: 198 1623 1210 2916
Fragments arranged by size:
2916 1623 1210 198
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2425 2514 3036
Size: 107 122 71 631 605 753 89 522
Cuts at: 3036 4558 136
Size: 1522 1525
Fragments arranged by size:
1525 1522 753 631 605 522 122 107
89 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 2111 2484 3364 2111
Size: 373 880 4694
Fragments arranged by size:
4694 880 373
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2510 2993 4008 5164 1790
Size: 300 420 483 1015 1156 2573
Fragments arranged by size:
2573 1156 1015 483 420 300
BmgBI CAC'GTC
Cuts at: 1318 2676 3244 1318
Size: 1358 568 4021
Fragments arranged by size:
4021 1358 568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 3678 4636
Size: 416 53 83 399 24 1625 636 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1625 958 636 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 4607 1239
Size: 1292 2076 2579
Fragments arranged by size:
2579 2076 1292
Bpu10I CC'TnA_GC
Cuts at: 2241 2241
Size: 5947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2776 4610 882
Size: 617 1277 1834 2219
Fragments arranged by size:
2219 1834 1277 617
BsaAI yAC'GTr
Cuts at: 493 1204 2887 3738 493
Size: 711 1683 851 2702
Fragments arranged by size:
2702 1683 851 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2867 3207 3754
Size: 53 83 186 936 614 720 340 547
Cuts at: 3754 4136 4771 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 720 635 614 547 382 340
186 83 53
BsaWI w'CCGG_w
Cuts at: 3013 3155 4380 4778 5052 5199 5610 3013
Size: 142 1225 398 274 147 411 3350
Fragments arranged by size:
3350 1225 411 398 274 147 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3197 3227 1178
Size: 30 165 30 1794 30 3898
Fragments arranged by size:
3898 1794 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 3056 1186
Size: 1189 681 4077
Fragments arranged by size:
4077 1189 681
BseYI C'CCAG_C
Cuts at: 2083 2308 2503 2873 5150 5646 2083
Size: 225 195 370 2277 496 2384
Fragments arranged by size:
2384 2277 496 370 225 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 2402 2456 3089 1163
Size: 573 531 135 54 633 4021
Fragments arranged by size:
4021 633 573 531 135 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2172 2216 2420 2499 2521 2748
Size: 275 186 171 44 204 79 22 227
Cuts at: 2748 3397 4158 5186 1540
Size: 649 761 1028 2301
Fragments arranged by size:
2301 1028 761 649 275 227 204 186
171 79 44 22
BsiHKAI G_wGCw'C
Cuts at: 2167 3066 4008 4093 5164 2167
Size: 899 942 85 1071 2950
Fragments arranged by size:
2950 1071 942 899 85
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2117 2167 2510 2838 2909 2993
Size: 399 300 27 50 343 328 71 84
Cuts at: 2993 3066 3266 4008 4093 5164 1391
Size: 73 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 328 300 200
85 84 73 71 50 27
BspEI T'CCGG_A
Cuts at: 3013 5610 3013
Size: 2597 3350
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2289 2425 2758 3302 844
Size: 1445 136 333 544 3489
Fragments arranged by size:
3489 1445 544 333 136
BsrBI CCG'CTC
Cuts at: 1394 1823 2371 2644 3834 1394
Size: 429 548 273 1190 3507
Fragments arranged by size:
3507 1190 548 429 273
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2149 2167 2415 2573 2747 2804 4591
Size: 110 339 18 248 158 174 57 1787
Cuts at: 4591 4778 1700
Size: 187 2869
Fragments arranged by size:
2869 1787 339 248 187 174 158 110
57 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 2058 2634 1925
Size: 133 576 5238
Fragments arranged by size:
5238 576 133
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 794
Size: 1595 4352
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942
Size: 1305 367 385 17 1452 86 12 346
Cuts at: 5942 1972
Size: 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2067 2178 2420 2529 2838 3178 3216
Size: 552 1002 111 242 109 309 340 38
Cuts at: 3216 513
Size: 3244
Fragments arranged by size:
3244 1002 552 340 309 242 111 109
38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2915 487
Size: 683 56 1689 3519
Fragments arranged by size:
3519 1689 683 56
BtsI GCAGTG_nn'
Cuts at: 3020 3463 4257 4277 3020
Size: 443 794 20 4690
Fragments arranged by size:
4690 794 443 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DraIII CAC_nnn'GTG
Cuts at: 3112 3112
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2169 2213
Size: 54 1425 50 275 18 246 93 44
Cuts at: 2213 2417 2496 2518 2526 2605 2745 3394 4283
Size: 204 79 22 8 79 140 649 889
Cuts at: 4283 5912 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 649 275 246 204 140
93 79 79 54 50 44 43 22
18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2169 2213 2417 2496 2518 2745 3394
Size: 275 357 44 204 79 22 227 649
Cuts at: 3394 1537
Size: 4090
Fragments arranged by size:
4090 649 357 275 227 204 79 44
22
EarI CTCTTCn'nnn_
Cuts at: 1353 3877 1353
Size: 2524 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 4534 4908 5054 387
Size: 1064 138 765 2180 374 146 1280
Fragments arranged by size:
2180 1280 1064 765 374 146 138
EcoICRI GAG'CTC
Cuts at: 3064 3064
Size: 5947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2531 4010 4607 5394 5662 1239
Size: 393 836 63 1479 597 787 268 1524
Fragments arranged by size:
1524 1479 836 787 597 393 268 63
EcoNI CCTnn'n_nnAGG
Cuts at: 3071 3071
Size: 5947
EcoO109I rG'GnC_Cy
Cuts at: 2087 2507 2843 2990 2087
Size: 420 336 147 5044
Fragments arranged by size:
5044 420 336 147
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 2150 2330 2870 2880 5094 1678
Size: 472 180 540 10 2214 2531
Fragments arranged by size:
2531 2214 540 472 180 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3197 3229 4588 4620 4662
Size: 32 135 32 1625 32 1359 32 42
Cuts at: 4662 4694 1373
Size: 32 2626
Fragments arranged by size:
2626 1625 1359 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5947
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KasI G'GCGC_C
Cuts at: 2146 2866 2146
Size: 720 5227
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3228 5061 5245 1305
Size: 1923 1833 184 2007
Fragments arranged by size:
2007 1923 1833 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 10
Size: 54 1425 343 696 3429
Fragments arranged by size:
3429 1425 696 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 3905 4264 4423 518
Size: 648 966 1773 359 159 2042
Fragments arranged by size:
2042 1773 966 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2160 2180 2422 2584 2683 2840 3218
Size: 463 91 20 242 162 99 157 378
Cuts at: 3218 3363 4040 5188 5433 1606
Size: 145 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 463 378 245 242 162
157 145 99 91 20
NaeI GCC'GGC
Cuts at: 2151 2575 2749 2151
Size: 424 174 5349
Fragments arranged by size:
5349 424 174
NarI GG'CG_CC
Cuts at: 2147 2867 2147
Size: 720 5227
NcoI C'CATG_G
Cuts at: 513 1065 2529 513
Size: 552 1464 3931
Fragments arranged by size:
3931 1464 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NgoMIV G'CCGG_C
Cuts at: 2149 2573 2747 2149
Size: 424 174 5349
Fragments arranged by size:
5349 424 174
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 2496 2518 3394 2496
Size: 22 876 5049
Fragments arranged by size:
5049 876 22
NspI r_CATG'y
Cuts at: 1132 1229 2392 3305 4850 1132
Size: 97 1163 913 1545 2229
Fragments arranged by size:
2229 1545 1163 913 97
PasI CC'CwG_GG
Cuts at: 1348 2097 1348
Size: 749 5198
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2629 1108
Size: 1521 4426
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PmlI CAC'GTG
Cuts at: 2887 2887
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2843 2843
Size: 5947
PshAI GACnn'nnGTC
Cuts at: 1613 3001 3048 1613
Size: 1388 47 4512
Fragments arranged by size:
4512 1388 47
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PspOMI G'GGCC_C
Cuts at: 2086 2506 2989 2086
Size: 420 483 5044
Fragments arranged by size:
5044 483 420
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SacI G_AGCT'C
Cuts at: 3066 3066
Size: 5947
SacII CC_GC'GG
Cuts at: 2070 2181 2423 2841 3219 2070
Size: 111 242 418 378 4798
Fragments arranged by size:
4798 418 378 242 111
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SanDI GG'GwC_CC
Cuts at: 2843 2843
Size: 5947
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2755 3295 4430 5111 5302 826
Size: 219 1710 540 1135 681 191 1471
Fragments arranged by size:
1710 1471 1135 681 540 219 191
SfoI GGC'GCC
Cuts at: 2148 2868 2148
Size: 720 5227
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 2149 1700
Size: 449 5498
SmaI CCC'GGG
Cuts at: 1872 2731 2914 2988 3274 1872
Size: 859 183 74 286 4545
Fragments arranged by size:
4545 859 286 183 74
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 2190 2529 3386 513
Size: 552 1125 339 857 3074
Fragments arranged by size:
3074 1125 857 552 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2887 3974 3991 4144 4329 4774 325
Size: 1255 1307 1087 17 153 185 445 1498
Fragments arranged by size:
1498 1307 1255 1087 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2889 4193 5905
Size: 274 80 33 51 1443 912 1304 1712
Cuts at: 5905 96
Size: 138
Fragments arranged by size:
1712 1443 1304 912 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2536 3193 2536
Size: 657 5290
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 2729 2912 2986 3272 1870
Size: 859 183 74 286 4545
Fragments arranged by size:
4545 859 286 183 74
XmnI GAAnn'nnTTC
Cuts at: 2225 4076 2225
Size: 1851 4096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AvrII BbvCI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PfoI PspXI PsrI PvuII RsrII SapI SexAI
SfiI SrfI SwaI