Restriction map for
pFN21AB9719
(Circular) (Six-base) MAPSORT of: pFN21AB9719.seq Check: 2297 from: 1 to: 5911
pFN21AB9719 5911 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2364 2311
Size: 53 5858
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3195 3254 1937
Size: 1258 59 4594
Fragments arranged by size:
4594 1258 59
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 2555 2684 3974 5358 5626 1632
Size: 580 343 129 1290 1384 268 1917
Fragments arranged by size:
1917 1384 1290 580 343 268 129
AfeI AGC'GCT
Cuts at: 2222 2273 2542 2687 2717 2222
Size: 51 269 145 30 5416
Fragments arranged by size:
5416 269 145 51 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 4756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2382 1184
Size: 1198 4713
AlwNI CAG_nnn'CTG
Cuts at: 2199 3654 5226 2199
Size: 1455 1572 2884
Fragments arranged by size:
2884 1572 1455
ApaI G_GGCC'C
Cuts at: 2030 2822 3084 2030
Size: 792 262 4857
Fragments arranged by size:
4857 792 262
ApaLI G'TGCA_C
Cuts at: 3968 5124 3968
Size: 1156 4755
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2567 2567
Size: 5911
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2878 3064 3236 1870
Size: 73 91 844 186 172 4545
Fragments arranged by size:
4545 844 186 172 91 73
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3232 4687 618
Size: 325 217 627 1445 1455 1842
Fragments arranged by size:
1842 1455 1445 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2822 2887 3073 3084 3230 1391
Size: 639 792 65 186 11 146 4072
Fragments arranged by size:
4072 792 639 186 146 65 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4940
BbvCI CC'TCA_GC
Cuts at: 2778 2778
Size: 5911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3146 3180 4102 4136 1903
Size: 34 1209 34 922 34 3678
Fragments arranged by size:
3678 1209 922 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2304 2922
Size: 107 122 71 631 605 337 295 618
Cuts at: 2922 3003 4522 136
Size: 81 1519 1525
Fragments arranged by size:
1525 1519 631 618 605 337 295 122
107 81 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2822 3063 3084 3972 5128 1790
Size: 240 792 241 21 888 1156 2573
Fragments arranged by size:
2573 1156 888 792 241 240 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 3642 4600
Size: 416 53 83 399 24 1694 531 958
Cuts at: 4600 442
Size: 1753
Fragments arranged by size:
1753 1694 958 531 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2066 1056
Size: 1010 4901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 2924 3007 4571 1239
Size: 1307 378 83 1564 2579
Fragments arranged by size:
2579 1564 1307 378 83
Bpu10I CC'TnA_GC
Cuts at: 2778 2778
Size: 5911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2171 4036 4901 5199 2135
Size: 36 1865 865 298 2847
Fragments arranged by size:
2847 1865 865 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2788 4574 882
Size: 617 1289 1786 2219
Fragments arranged by size:
2219 1786 1289 617
BsaAI yAC'GTr
Cuts at: 493 1204 2575 3702 493
Size: 711 1371 1127 2702
Fragments arranged by size:
2702 1371 1127 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 3718 4100 4735
Size: 53 83 186 936 1140 1045 382 635
Cuts at: 4735 275
Size: 1451
Fragments arranged by size:
1451 1140 1045 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5686
Fragments arranged by size:
5686 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3008 1186
Size: 1822 4089
BseYI C'CCAG_C
Cuts at: 2023 2302 2688 3009 5114 5610 2023
Size: 279 386 321 2105 496 2324
Fragments arranged by size:
2324 2105 496 386 321 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2212 2342 2642 1163
Size: 573 476 130 300 4432
Fragments arranged by size:
4432 573 476 300 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2498 2680 3361 4122 5150 1540
Size: 275 186 497 182 681 761 1028 2301
Fragments arranged by size:
2301 1028 761 681 497 275 186 182
BsiHKAI G_wGCw'C
Cuts at: 3972 4057 5128 3972
Size: 85 1071 4755
Fragments arranged by size:
4755 1071 85
BsmI GAATG_Cn'
Cuts at: 3422 3515 3422
Size: 93 5818
BsmBI CGTCTCn'nnnn_
Cuts at: 2522 2591 2522
Size: 69 5842
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2822 2887 3063 3073 3084 3230
Size: 399 240 792 65 176 10 11 146
Cuts at: 3230 3972 4057 5128 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 792 742 399 240 176 146
85 65 11 10
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 2097 2311 2364 2614 3266 844
Size: 1220 33 214 53 250 652 3489
Fragments arranged by size:
3489 1220 652 250 214 53 33
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 2563 2675 2679 2871 4555 4742
Size: 110 620 133 112 4 192 1684 187
Cuts at: 4742 1700
Size: 2869
Fragments arranged by size:
2869 1684 620 192 187 133 112 110
4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 2012 2567 1925
Size: 87 555 5269
Fragments arranged by size:
5269 555 87
BssSI C'ACGA_G
Cuts at: 2435 3971 4983 2435
Size: 1536 1012 3363
Fragments arranged by size:
3363 1536 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2199 2326 794
Size: 1405 127 4379
Fragments arranged by size:
4379 1405 127
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 3149 1184
Size: 1965 3946
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2524 2323
Size: 201 5710
BstYI r'GATC_y
Cuts at: 1972 2100 3241 3608 3993 4010 5462 5548 5560
Size: 128 1141 367 385 17 1452 86 12
Cuts at: 5560 5906 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1141 385 367 346 128 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2397 2484 2722 3132 513
Size: 552 939 393 87 238 410 3292
Fragments arranged by size:
3292 939 552 410 393 238 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2066 2359 3427 4221 4241 2066
Size: 293 1068 794 20 3736
Fragments arranged by size:
3736 1068 794 293 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2780 4918 809
Size: 507 1464 2138 1802
Fragments arranged by size:
2138 1802 1464 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2394 2487
Size: 54 1425 50 275 18 384 180 93
Cuts at: 2487 2495 2677 2823 3358 4247 5876 8
Size: 8 182 146 535 889 1629 43
Fragments arranged by size:
1629 1425 889 535 384 275 182 180
146 93 54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2495 2677 3358 1537
Size: 275 683 182 681 4090
Fragments arranged by size:
4090 683 681 275 182
EarI CTCTTCn'nnn_
Cuts at: 1353 2531 2651 3841 1353
Size: 1178 120 1190 3423
Fragments arranged by size:
3423 1190 1178 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 2462 2825 4498 4872 5018
Size: 1064 138 510 363 363 1673 374 146
Cuts at: 5018 387
Size: 1280
Fragments arranged by size:
1673 1280 1064 510 374 363 363 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2546 2555 2684 2924 3007 3974
Size: 393 580 334 9 129 240 83 967
Cuts at: 3974 4571 5358 5626 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 967 787 597 580 393 334 268
240 129 83 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2420 2420
Size: 5911
EcoO109I rG'GnC_Cy
Cuts at: 3081 3081
Size: 5911
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 5911
FseI GG_CCGG'CC
Cuts at: 2875 2875
Size: 5911
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2224 2275 2544 2689 2719 5058 1678
Size: 546 51 269 145 30 2339 2531
Fragments arranged by size:
2531 2339 546 269 145 51 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373
Size: 32 135 32 2980 32 42 32 2626
Fragments arranged by size:
2980 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2718 5025 5209 1305
Size: 1413 2307 184 2007
Fragments arranged by size:
2307 2007 1413 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 10
Size: 54 1425 343 564 3525
Fragments arranged by size:
3525 1425 564 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2615 3095 3869 4228 4387 518
Size: 648 1155 294 480 774 359 159 2042
Fragments arranged by size:
2042 1155 774 648 480 359 294 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2302 2392 2524 2724 3144 3327 4004
Size: 501 195 90 132 200 420 183 677
Cuts at: 4004 5152 5397 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 420 245 200 195
183 132 90
NaeI GCC'GGC
Cuts at: 2432 2565 2677 2681 2873 2432
Size: 133 112 4 192 5470
Fragments arranged by size:
5470 192 133 112 4
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4420
Fragments arranged by size:
4420 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NgoMIV G'CCGG_C
Cuts at: 2430 2563 2675 2679 2871 2430
Size: 133 112 4 192 5470
Fragments arranged by size:
5470 192 133 112 4
NheI G'CTAG_C
Cuts at: 1052 2062 1052
Size: 1010 4901
NotI GC'GGCC_GC
Cuts at: 2495 3358 2495
Size: 863 5048
NspI r_CATG'y
Cuts at: 1132 1229 2614 3269 4814 1132
Size: 97 1385 655 1545 2229
Fragments arranged by size:
2229 1545 1385 655 97
PasI CC'CwG_GG
Cuts at: 1348 2887 1348
Size: 1539 4372
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 1108
Size: 1758 4153
PfoI T'CCnGG_A
Cuts at: 2133 2133
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PmlI CAC'GTG
Cuts at: 2575 2575
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PspOMI G'GGCC_C
Cuts at: 2026 2818 3080 2026
Size: 792 262 4857
Fragments arranged by size:
4857 792 262
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2302 2392 2524 2302
Size: 90 132 5689
Fragments arranged by size:
5689 132 90
SacII CC_GC'GG
Cuts at: 2725 2725
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SapI GCTCTTCn'nnn_
Cuts at: 2531 2531
Size: 5911
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 3259 4394 5075 5266 826
Size: 219 2214 1135 681 191 1471
Fragments arranged by size:
2214 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2922 2922
Size: 5911
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 2036 3238 1872
Size: 164 1202 4545
Fragments arranged by size:
4545 1202 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2186 4015 4916 5178 820
Size: 197 926 207 36 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 207 197 36
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 2864 2916 1517
Size: 1347 52 4512
Fragments arranged by size:
4512 1347 52
StyI C'CwwG_G
Cuts at: 513 1065 2004 2649 2805 2865 3350 513
Size: 552 939 645 156 60 485 3074
Fragments arranged by size:
3074 939 645 552 485 156 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 3103 3938 3955 4108 4293 4738
Size: 1255 1293 230 835 17 153 185 445
Cuts at: 4738 325
Size: 1498
Fragments arranged by size:
1498 1293 1255 835 445 230 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5869 96
Size: 274 80 33 51 1443 2180 1712 138
Fragments arranged by size:
2180 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2863 3097 2863
Size: 234 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 2034 3236 1870
Size: 164 1202 4545
Fragments arranged by size:
4545 1202 164
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FspAI KasI NarI NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacI SanDI SexAI
SfoI SrfI SwaI