Restriction map for
pFN21AB9715
(Circular) (Six-base) MAPSORT of: pFN21AB9715.seq Check: 6278 from: 1 to: 5881
pFN21AB9715 5881 bp
With 182 enzymes: SgfI *
March 28, 2012 12:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2906 3944 5328 5596 1632
Size: 1274 1038 1384 268 1917
Fragments arranged by size:
1917 1384 1274 1038 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 2840 4780 5757 1203
Size: 1637 1940 977 1327
Fragments arranged by size:
1940 1637 1327 977
AgeI A'CCGG_T
Cuts at: 2672 4712 2672
Size: 2040 3841
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2383 2501 3010 3133 3624 5196 2383
Size: 118 509 123 491 1572 3068
Fragments arranged by size:
3068 1572 509 491 123 118
ApaI G_GGCC'C
Cuts at: 2784 2784
Size: 5881
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 3190 3416 3568 5677 1493
Size: 1697 226 152 2109 1697
Fragments arranged by size:
2109 1697 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2487 3206 1870
Size: 73 544 719 4545
Fragments arranged by size:
4545 719 544 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2188 2266 2483 2491
Size: 325 217 627 318 83 78 217 8
Cuts at: 2491 2720 3202 4657 618
Size: 229 482 1455 1842
Fragments arranged by size:
1842 1455 627 482 325 318 229 217
217 83 78 8
BanII G_rGCy'C
Cuts at: 1391 2708 2768 2784 3200 1391
Size: 1317 60 16 416 4072
Fragments arranged by size:
4072 1317 416 60 16
BbeI G_GCGC'C
Cuts at: 2109 2192 2270 2495 2109
Size: 83 78 225 5495
Fragments arranged by size:
5495 225 83 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BbvCI CC'TCA_GC
Cuts at: 2084 2253 2084
Size: 169 5712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4072 4106 1903
Size: 34 2135 34 3678
Fragments arranged by size:
3678 2135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2624 4492 136
Size: 107 122 71 631 605 952 1868 1525
Fragments arranged by size:
1868 1525 952 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3048 5876 3048
Size: 2828 3053
BlpI GC'TnA_GC
Cuts at: 2767 3298 2767
Size: 531 5350
Bme1580I G_kGCm'C
Cuts at: 1790 2486 2723 2784 3942 5098 1790
Size: 696 237 61 1158 1156 2573
Fragments arranged by size:
2573 1158 1156 696 237 61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3612 4570 442
Size: 416 53 83 399 24 2195 958 1753
Fragments arranged by size:
2195 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4541 1239
Size: 3302 2579
Bpu10I CC'TnA_GC
Cuts at: 2084 2090 2253 2084
Size: 6 163 5712
Fragments arranged by size:
5712 163 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2942 4006 4871 5169 2942
Size: 1064 865 298 3654
Fragments arranged by size:
3654 1064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2457 4544 882
Size: 617 958 2087 2219
Fragments arranged by size:
2219 2087 958 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 3047 5875 3047
Size: 2828 3053
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2189 2267 2492
Size: 53 83 186 936 573 83 78 225
Cuts at: 2492 3688 4070 4705 275
Size: 1196 382 635 1451
Fragments arranged by size:
1451 1196 936 635 573 382 225 186
83 83 78 53
BsaWI w'CCGG_w
Cuts at: 2672 2864 4314 4712 4986 5133 5544 2672
Size: 192 1450 398 274 147 411 3009
Fragments arranged by size:
3009 1450 411 398 274 192 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2130 2160 2412 2442 3128
Size: 30 165 30 727 30 252 30 686
Cuts at: 3128 3158 1178
Size: 30 3901
Fragments arranged by size:
3901 727 686 252 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2418 2430 2433 1186
Size: 1232 12 3 4634
Fragments arranged by size:
4634 1232 12 3
BseYI C'CCAG_C
Cuts at: 2295 2553 2588 5084 5580 2295
Size: 258 35 2496 496 2596
Fragments arranged by size:
2596 2496 496 258 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2821 3098 1163
Size: 573 549 536 277 3946
Fragments arranged by size:
3946 573 549 536 277
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2619 2649 2921 2940 3331 4092
Size: 275 186 618 30 272 19 391 761
Cuts at: 4092 5120 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 618 391 275 272 186
30 19
BsiHKAI G_wGCw'C
Cuts at: 2739 3942 4027 5098 2739
Size: 1203 85 1071 3522
Fragments arranged by size:
3522 1203 1071 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2486 2708 2723 2739 2768 2784 3200
Size: 399 696 222 15 16 29 16 416
Cuts at: 3200 3942 4027 5098 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 416 399 222 85
29 16 16 15
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2447 3236 844
Size: 1603 789 3489
Fragments arranged by size:
3489 1603 789
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2149 2567 2672 4525 4712 1700
Size: 110 339 418 105 1853 187 2869
Fragments arranged by size:
2869 1853 418 339 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 3135 96
Size: 3039 2842
BssHII G'CGCG_C
Cuts at: 1925 3067 1925
Size: 1142 4739
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 3048 3211 3578 3963 3980 5432 5518 5530
Size: 1076 163 367 385 17 1452 86 12
Cuts at: 5530 5876 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1076 385 367 346 163 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2336 2336
Size: 5881
BtgI C'CryG_G
Cuts at: 513 1065 2004 2184 2619 2784 2921 3153 513
Size: 552 939 180 435 165 137 232 3241
Fragments arranged by size:
3241 939 552 435 232 180 165 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2148 487
Size: 683 56 922 4220
Fragments arranged by size:
4220 922 683 56
BtsI GCAGTG_nn'
Cuts at: 2634 2692 3397 4191 4211 2634
Size: 58 705 794 20 4304
Fragments arranged by size:
4304 794 705 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2616 2646
Size: 54 1425 50 275 18 567 219 30
Cuts at: 2646 2918 2937 3328 4217 5846 8
Size: 272 19 391 889 1629 43
Fragments arranged by size:
1629 1425 889 567 391 275 272 219
54 50 43 30 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2616 2646 2918 2937 3328 1537
Size: 275 804 30 272 19 391 4090
Fragments arranged by size:
4090 804 391 275 272 30 19
EarI CTCTTCn'nnn_
Cuts at: 1353 3811 1353
Size: 2458 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2978 4468 4842 4988 387
Size: 1064 138 1389 1490 374 146 1280
Fragments arranged by size:
1490 1389 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2906 3944 4541 5328 5596 1239
Size: 393 1274 1038 597 787 268 1524
Fragments arranged by size:
1524 1274 1038 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3014 3014
Size: 5881
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 2109 2192 2270 2495 2502 3113 5028 1678
Size: 431 83 78 225 7 611 1915 2531
Fragments arranged by size:
2531 1915 611 431 225 83 78 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2083 2115 2412 2444 2454
Size: 32 135 32 511 32 297 32 10
Cuts at: 2454 2486 2843 2875 4522 4554 4596 4628 1373
Size: 32 357 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 511 357 297 135 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KasI G'GCGC_C
Cuts at: 2105 2188 2266 2491 2105
Size: 83 78 225 5495
Fragments arranged by size:
5495 225 83 78
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4995 5179 1305
Size: 3690 184 2007
Fragments arranged by size:
3690 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3839 4198 4357 518
Size: 648 2673 359 159 2042
Fragments arranged by size:
2673 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2121 2127 2186 2291 2299 2621 2923
Size: 506 9 6 59 105 8 322 302
Cuts at: 2923 2947 2998 3297 3974 5122 5367 1606
Size: 24 51 299 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 506 322 302 299 245
105 59 51 24 9 8 6
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 5881
NarI GG'CG_CC
Cuts at: 2106 2189 2267 2492 2106
Size: 83 78 225 5495
Fragments arranged by size:
5495 225 83 78
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4390
Fragments arranged by size:
4390 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 2616 2646 2918 3328 2616
Size: 30 272 410 5169
Fragments arranged by size:
5169 410 272 30
NspI r_CATG'y
Cuts at: 1132 1229 3239 4784 1132
Size: 97 2010 1545 2229
Fragments arranged by size:
2229 2010 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3127 1348
Size: 1779 4102
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 4510
PfoI T'CCnGG_A
Cuts at: 3159 3159
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3012 3044 3950 3982 5463 5495 3012
Size: 32 906 32 1481 32 3398
Fragments arranged by size:
3398 1481 906 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PspOMI G'GGCC_C
Cuts at: 2780 2780
Size: 5881
PstI C_TGCA'G
Cuts at: 830 2952 3233 830
Size: 2122 281 3478
Fragments arranged by size:
3478 2122 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2299 2947 2299
Size: 648 5233
RsrII CG'GwC_CG
Cuts at: 2867 2925 2867
Size: 58 5823
SacII CC_GC'GG
Cuts at: 2187 2622 2924 2187
Size: 435 302 5144
Fragments arranged by size:
5144 435 302
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2948 3229 4364 5045 5236 826
Size: 219 1903 281 1135 681 191 1471
Fragments arranged by size:
1903 1471 1135 681 281 219 191
SfoI GGC'GCC
Cuts at: 2107 2190 2268 2493 2107
Size: 83 78 225 5495
Fragments arranged by size:
5495 225 83 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 2567 2672 1700
Size: 867 105 4909
Fragments arranged by size:
4909 867 105
SmaI CCC'GGG
Cuts at: 1872 2489 3208 1872
Size: 617 719 4545
Fragments arranged by size:
4545 719 617
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2957 3985 4886 5148 820
Size: 197 926 1014 1028 901 262 1553
Fragments arranged by size:
1553 1028 1014 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 3320 513
Size: 552 939 1316 3074
Fragments arranged by size:
3074 1316 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 3908 3925 4078 4263 4708 325
Size: 1255 1238 1090 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1238 1090 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 3135 4127
Size: 274 80 33 51 1443 855 303 992
Cuts at: 4127 5839 96
Size: 1712 138
Fragments arranged by size:
1712 1443 992 855 303 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2130 2162 5841 5873 2130
Size: 32 3679 32 2138
Fragments arranged by size:
3679 2138 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2468 1316
Size: 1152 4729
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 2487 3206 1870
Size: 617 719 4545
Fragments arranged by size:
4545 719 617
XmnI GAAnn'nnTTC
Cuts at: 4010 4010
Size: 5881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PpuMI
PspXI PsrI SacI SanDI SapI SexAI SfiI SrfI
SwaI XcmI