Restriction map for
pFN21AB9714
(Circular) (Six-base) MAPSORT of: pFN21AB9714.seq Check: 219 from: 1 to: 5851
pFN21AB9714 5851 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2228 2228
Size: 5851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 2922 3194 1937
Size: 409 576 272 4594
Fragments arranged by size:
4594 576 409 272
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 2435 3914 5298 5566 1632
Size: 743 60 1479 1384 268 1917
Fragments arranged by size:
1917 1479 1384 743 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 2166 2812 2902 3077 3594 5166 2166
Size: 646 90 175 517 1572 2851
Fragments arranged by size:
2851 1572 646 517 175 90
ApaI G_GGCC'C
Cuts at: 2335 2335
Size: 5851
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3133 3160 3386 3538 5647 1493
Size: 1640 27 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152 27
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 3176 1870
Size: 73 1233 4545
Fragments arranged by size:
4545 1233 73
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2993 3172 4627 618
Size: 325 217 627 1206 179 1455 1842
Fragments arranged by size:
1842 1455 1206 627 325 217 179
BanII G_rGCy'C
Cuts at: 1391 2096 2208 2335 2483 2819 3003 3170 1391
Size: 705 112 127 148 336 184 167 4072
Fragments arranged by size:
4072 705 336 184 167 148 127 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4880
BbvCI CC'TCA_GC
Cuts at: 2562 2562
Size: 5851
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4042 4076 1903
Size: 34 50 34 2021 34 3678
Fragments arranged by size:
3678 2021 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2662 3743 4953 2662
Size: 1081 1210 3560
Fragments arranged by size:
3560 1210 1081
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2543 4462 136
Size: 107 122 71 631 605 871 1919 1525
Fragments arranged by size:
1919 1525 871 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 2468 2813 3268 2468
Size: 345 455 5051
Fragments arranged by size:
5051 455 345
Bme1580I G_kGCm'C
Cuts at: 1790 2335 3912 5068 1790
Size: 545 1577 1156 2573
Fragments arranged by size:
2573 1577 1156 545
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2700 2838 2700
Size: 138 5713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2991 4511 1239
Size: 992 760 1520 2579
Fragments arranged by size:
2579 1520 992 760
Bpu10I CC'TnA_GC
Cuts at: 2354 2562 2354
Size: 208 5643
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3976 4841 5139 3976
Size: 865 298 4688
Fragments arranged by size:
4688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 2954 2967 4514 882
Size: 617 708 747 13 1547 2219
Fragments arranged by size:
2219 1547 747 708 617 13
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4040 4675 275
Size: 53 83 186 936 2125 382 635 1451
Fragments arranged by size:
2125 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2883 2913 3017 3047 1178
Size: 30 165 30 1480 30 104 30 3982
Fragments arranged by size:
3982 1480 165 104 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2059 1186
Size: 856 17 4978
Fragments arranged by size:
4978 856 17
BseYI C'CCAG_C
Cuts at: 2077 2482 2846 3077 5054 5550 2077
Size: 405 364 231 1977 496 2378
Fragments arranged by size:
2378 1977 496 405 364 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 1163
Size: 573 468 4810
Fragments arranged by size:
4810 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3301 4062 5090 1540
Size: 275 186 48 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2819 3912 3997 5068 2819
Size: 1093 85 1071 3602
Fragments arranged by size:
3602 1093 1071 85
BsmI GAATG_Cn'
Cuts at: 2630 3362 3455 2630
Size: 732 93 5026
Fragments arranged by size:
5026 732 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2208 2335 2483 2819 3003 3170
Size: 399 306 112 127 148 336 184 167
Cuts at: 3170 3912 3997 5068 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 336 306 184 167
148 127 112 85
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 2862 3740 5470 2862
Size: 878 1730 3243
Fragments arranged by size:
3243 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2228 3206 844
Size: 1384 978 3489
Fragments arranged by size:
3489 1384 978
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 2458 3738 1394
Size: 429 264 371 1280 3507
Fragments arranged by size:
3507 1280 429 371 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4495 4682 1700
Size: 110 2685 187 2869
Fragments arranged by size:
2869 2685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2182 794
Size: 1388 4463
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstXI CCAn_nnnn'nTGG
Cuts at: 2969 2969
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 2932 3181 3548 3933 3950 5402 5488 5500
Size: 960 249 367 385 17 1452 86 12
Cuts at: 5500 5846 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 960 385 367 346 249 86
17 12
BstZ17I GTA'TAC
Cuts at: 2347 2347
Size: 5851
BtgI C'CryG_G
Cuts at: 513 1065 2539 513
Size: 552 1474 3825
Fragments arranged by size:
3825 1474 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2290 487
Size: 683 56 1064 4048
Fragments arranged by size:
4048 1064 683 56
BtsI GCAGTG_nn'
Cuts at: 2183 2192 3367 4161 4181 2183
Size: 9 1175 794 20 3853
Fragments arranged by size:
3853 1175 794 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 2904 3298
Size: 54 1425 50 275 18 933 141 394
Cuts at: 3298 4187 5816 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 933 889 394 275 141 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 3781 1353
Size: 980 1448 3423
Fragments arranged by size:
3423 1448 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4438 4812 4958 387
Size: 1064 138 2849 374 146 1280
Fragments arranged by size:
2849 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2817 2817
Size: 5851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2375 2435 2991 3914 4511 5298
Size: 393 599 144 60 556 923 597 787
Cuts at: 5298 5566 1239
Size: 268 1524
Fragments arranged by size:
1524 923 787 599 597 556 393 268
144 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2417 2417
Size: 5851
EcoRI G'AATT_C
Cuts at: 3133 3160 3538 3133
Size: 27 378 5446
Fragments arranged by size:
5446 378 27
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 2587 4998 1678
Size: 909 2411 2531
Fragments arranged by size:
2531 2411 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2934 2966 3017 3049 4492
Size: 32 135 32 1362 32 51 32 1443
Cuts at: 4492 4524 4566 4598 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 1362 135 51 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2965 4965 5149 1305
Size: 1660 2000 184 2007
Fragments arranged by size:
2007 2000 1660 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 2906 10
Size: 54 1425 343 933 141 2955
Fragments arranged by size:
2955 1425 933 343 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2988 3809 4168 4327 518
Size: 648 1822 821 359 159 2042
Fragments arranged by size:
2042 1822 821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2428 3267 3944 5092 5337 1606
Size: 516 306 839 677 1148 245 2120
Fragments arranged by size:
2120 1148 839 677 516 306 245
NcoI C'CATG_G
Cuts at: 513 1065 2539 513
Size: 552 1474 3825
Fragments arranged by size:
3825 1474 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2983 3209 4754 1132
Size: 97 1754 226 1545 2229
Fragments arranged by size:
2229 1754 1545 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5851
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3016 1108
Size: 1908 3943
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2331 2331
Size: 5851
PstI C_TGCA'G
Cuts at: 830 2669 2695 3090 3203 830
Size: 1839 26 395 113 3478
Fragments arranged by size:
3478 1839 395 113 26
PvuI CG_AT'CG
Cuts at: 2001 2049 2001
Size: 48 5803
PvuII CAG'CTG
Cuts at: 2428 2428
Size: 5851
SacI G_AGCT'C
Cuts at: 2819 2819
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SbfI CC_TGCA'GG
Cuts at: 2669 3203 2669
Size: 534 5317
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 2665 2691 2923 3086 3199 4334 5015
Size: 219 1620 26 232 163 113 1135 681
Cuts at: 5015 5206 826
Size: 191 1471
Fragments arranged by size:
1620 1471 1135 681 232 219 191 163
113 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3955 4856 5118 820
Size: 197 926 2012 901 262 1553
Fragments arranged by size:
2012 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 2639 1517
Size: 1122 4729
StyI C'CwwG_G
Cuts at: 513 1065 2539 3290 513
Size: 552 1474 751 3074
Fragments arranged by size:
3074 1474 751 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2819 3038 3878 3895 4048 4233 4678
Size: 1255 1239 219 840 17 153 185 445
Cuts at: 4678 325
Size: 1498
Fragments arranged by size:
1498 1255 1239 840 445 219 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 3120 4097
Size: 274 80 33 51 1443 267 876 977
Cuts at: 4097 5809 96
Size: 1712 138
Fragments arranged by size:
1712 1443 977 876 274 267 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2883 2915 5811 5843 2883
Size: 32 2896 32 2891
Fragments arranged by size:
2896 2891 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2946 1316
Size: 1630 4221
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI