Restriction map for
pFN21AB9713
(Circular) (Six-base) MAPSORT of: pFN21AB9713.seq Check: 3767 from: 1 to: 5827
pFN21AB9713 5827 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3094 3094
Size: 5827
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2070 3637 278
Size: 53 83 186 936 534 1567 2468
Fragments arranged by size:
2468 1567 936 534 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3890 5274 5542 1632
Size: 2258 1384 268 1917
Fragments arranged by size:
2258 1917 1384 268
AfeI AGC'GCT
Cuts at: 2959 2959
Size: 5827
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 2104 2248 3570 5142 2104
Size: 144 1322 1572 2789
Fragments arranged by size:
2789 1572 1322 144
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2650 3136 3362 3514 5623 1493
Size: 1157 486 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 486 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2291 3152 1870
Size: 73 348 861 4545
Fragments arranged by size:
4545 861 348 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2540 3148 4603 618
Size: 325 217 627 262 491 608 1455 1842
Fragments arranged by size:
1842 1455 627 608 491 325 262 217
BanII G_rGCy'C
Cuts at: 1391 3146 1391
Size: 1755 4072
BbeI G_GCGC'C
Cuts at: 2053 2544 2053
Size: 491 5336
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4856
BbvCI CC'TCA_GC
Cuts at: 2274 2274
Size: 5827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2656 2690 4018 4052 1903
Size: 34 719 34 1328 34 3678
Fragments arranged by size:
3678 1328 719 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2863 3719 4929 2863
Size: 856 1210 3761
Fragments arranged by size:
3761 1210 856
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 3888 5044 1790
Size: 2098 1156 2573
Fragments arranged by size:
2573 2098 1156
BmgBI CAC'GTC
Cuts at: 1318 2470 1318
Size: 1152 4675
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3558 4516 442
Size: 416 53 83 399 24 2141 958 1753
Fragments arranged by size:
2141 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2984 3097 3111 4487 1239
Size: 1229 516 113 14 1376 2579
Fragments arranged by size:
2579 1376 1229 516 113 14
Bpu10I CC'TnA_GC
Cuts at: 2274 2274
Size: 5827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2779 3087 4490 882
Size: 617 1280 308 1403 2219
Fragments arranged by size:
2219 1403 1280 617 308
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2050 2067 2238 2541
Size: 53 83 186 936 517 17 171 303
Cuts at: 2541 2967 3634 4016 4651 275
Size: 426 667 382 635 1451
Fragments arranged by size:
1451 936 667 635 517 426 382 303
186 171 83 53 17
BsaWI w'CCGG_w
Cuts at: 2802 4260 4658 4932 5079 5490 2802
Size: 1458 398 274 147 411 3139
Fragments arranged by size:
3139 1458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2301 2331 1178
Size: 30 165 30 898 30 4674
Fragments arranged by size:
4674 898 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2322 2325 2471 2923 2997 1186
Size: 1136 3 146 452 74 4016
Fragments arranged by size:
4016 1136 452 146 74 3
BseYI C'CCAG_C
Cuts at: 2241 2717 2954 2960 5030 5526 2241
Size: 476 237 6 2070 496 2542
Fragments arranged by size:
2542 2070 496 476 237 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 1163
Size: 573 569 4685
Fragments arranged by size:
4685 573 569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2376 2591 3888 3973 5044 2175
Size: 201 215 1297 85 1071 2958
Fragments arranged by size:
2958 1297 1071 215 201 85
BsmI GAATG_Cn'
Cuts at: 2214 3338 3431 2214
Size: 1124 93 4610
Fragments arranged by size:
4610 1124 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2376 2591 3146 3888 3973 5044
Size: 399 385 201 215 555 742 85 1071
Cuts at: 5044 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 555 399 385 215 201
85
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3079 3094 3182 844
Size: 2235 15 88 3489
Fragments arranged by size:
3489 2235 88 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2315 3714 1394
Size: 429 492 1399 3507
Fragments arranged by size:
3507 1399 492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2327 4471 4658 1700
Size: 110 517 2144 187 2869
Fragments arranged by size:
2869 2144 517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 2059 3887 4899 2059
Size: 1828 1012 2987
Fragments arranged by size:
2987 1828 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2227 2403 3157 3524 3909 3926 5378 5464
Size: 255 176 754 367 385 17 1452 86
Cuts at: 5464 5476 5822 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 754 385 367 346 255 176
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2249 2517 2249
Size: 268 5559
BtgI C'CryG_G
Cuts at: 513 1065 2004 2045 2209 2874 513
Size: 552 939 41 164 665 3466
Fragments arranged by size:
3466 939 665 552 164 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2016 487
Size: 683 56 790 4298
Fragments arranged by size:
4298 790 683 56
BtsI GCAGTG_nn'
Cuts at: 2607 3343 4137 4157 2607
Size: 736 794 20 4277
Fragments arranged by size:
4277 794 736 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2863 2898 529
Size: 35 2299 35 3458
Fragments arranged by size:
3458 2299 35 35
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DraIII CAC_nnn'GTG
Cuts at: 2345 2345
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2085 2319
Size: 54 1425 50 275 18 177 78 234
Cuts at: 2319 2742 3274 4163 5792 8
Size: 423 532 889 1629 43
Fragments arranged by size:
1629 1425 889 532 423 275 234 177
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 2299 2730 2829 3757 1353
Size: 938 8 431 99 928 3423
Fragments arranged by size:
3423 938 928 431 99 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2662 4414 4788 4934 387
Size: 1064 138 1073 1752 374 146 1280
Fragments arranged by size:
1752 1280 1073 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2984 3097 3111 3890 4487 5274
Size: 393 836 516 113 14 779 597 787
Cuts at: 5274 5542 1239
Size: 268 1524
Fragments arranged by size:
1524 836 787 779 597 516 393 268
113 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2108 2483 3015 2108
Size: 375 532 4920
Fragments arranged by size:
4920 532 375
EcoO109I rG'GnC_Cy
Cuts at: 2887 2887
Size: 5827
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2053 2544 2565 2961 4974 1678
Size: 375 491 21 396 2013 2531
Fragments arranged by size:
2531 2013 491 396 375 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373
Size: 32 135 32 2896 32 42 32 2626
Fragments arranged by size:
2896 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2916 3171 3417 669
Size: 988 281 978 255 246 3079
Fragments arranged by size:
3079 988 978 281 255 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 2049 2540 2049
Size: 491 5336
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2431 4941 5125 1305
Size: 1126 2510 184 2007
Fragments arranged by size:
2510 2007 1126 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2087 2321 2744 10
Size: 54 1425 343 255 234 423 3093
Fragments arranged by size:
3093 1425 423 343 255 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 3785 4144 4303 518
Size: 648 1404 1215 359 159 2042
Fragments arranged by size:
2042 1404 1215 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2907 2950 3243 3920 5068 5313 1606
Size: 441 860 43 293 677 1148 245 2120
Fragments arranged by size:
2120 1148 860 677 441 293 245 43
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 5827
NarI GG'CG_CC
Cuts at: 2050 2541 2050
Size: 491 5336
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4336
Fragments arranged by size:
4336 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 2132 2266 3185 4730 1132
Size: 97 903 134 919 1545 2229
Fragments arranged by size:
2229 1545 919 903 134 97
PasI CC'CwG_GG
Cuts at: 1348 2891 3040 1348
Size: 1543 149 4135
Fragments arranged by size:
4135 1543 149
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 1108
Size: 996 4831
PfoI T'CCnGG_A
Cuts at: 2484 2484
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2887 2887
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 5795
PstI C_TGCA'G
Cuts at: 830 2076 3030 3179 830
Size: 1246 954 149 3478
Fragments arranged by size:
3478 1246 954 149
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2907 2907
Size: 5827
SacII CC_GC'GG
Cuts at: 2048 2048
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SanDI GG'GwC_CC
Cuts at: 2887 2887
Size: 5827
SapI GCTCTTCn'nnn_
Cuts at: 2730 2730
Size: 5827
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SexAI A'CCwGG_T
Cuts at: 2341 2341
Size: 5827
SfcI C'TryA_G
Cuts at: 826 1045 2072 2383 3026 3175 4310 4991 5182
Size: 219 1027 311 643 149 1135 681 191
Cuts at: 5182 826
Size: 1471
Fragments arranged by size:
1471 1135 1027 681 643 311 219 191
149
SfoI GGC'GCC
Cuts at: 2051 2542 2051
Size: 491 5336
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3931 4832 5094 820
Size: 197 926 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 2132 3185 1132
Size: 1000 1053 3774
Fragments arranged by size:
3774 1053 1000
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 2447 2506 1517
Size: 930 59 4838
Fragments arranged by size:
4838 930 59
StyI C'CwwG_G
Cuts at: 513 1065 2004 3266 513
Size: 552 939 1262 3074
Fragments arranged by size:
3074 1262 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3116 4073 5785
Size: 274 80 33 51 1443 1139 957 1712
Cuts at: 5785 96
Size: 138
Fragments arranged by size:
1712 1443 1139 957 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2301 2333 5787 5819 2301
Size: 32 3454 32 2309
Fragments arranged by size:
3454 2309 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 3008 2737
Size: 271 5556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2068 3635 276
Size: 53 83 186 936 534 1567 2468
Fragments arranged by size:
2468 1567 936 534 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI
SfiI SrfI SwaI