Restriction map for
pFN21AB9706
(Circular) (Six-base) MAPSORT of: pFN21AB9706.seq Check: 2922 from: 1 to: 5719
pFN21AB9706 5719 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AfeI AGC'GCT
Cuts at: 2813 2813
Size: 5719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 2868 4618 5595 1203
Size: 1665 1750 977 1327
Fragments arranged by size:
1750 1665 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 1184
Size: 882 4837
AlwNI CAG_nnn'CTG
Cuts at: 2676 3462 5034 2676
Size: 786 1572 3361
Fragments arranged by size:
3361 1572 786
ApaI G_GGCC'C
Cuts at: 2568 2747 2568
Size: 179 5540
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2561 2615 2749 3044 1870
Size: 73 618 54 134 295 4545
Fragments arranged by size:
4545 618 295 134 73 54
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 2480 3040 4495 618
Size: 325 217 627 644 49 560 1455 1842
Fragments arranged by size:
1842 1455 644 627 560 325 217 49
BanII G_rGCy'C
Cuts at: 1391 2568 2706 2747 3038 1391
Size: 1177 138 41 291 4072
Fragments arranged by size:
4072 1177 291 138 41
BbeI G_GCGC'C
Cuts at: 2435 2484 2435
Size: 49 5670
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 2369 2478
Size: 107 122 71 631 605 688 9 109
Cuts at: 2478 2517 4330 136
Size: 39 1813 1525
Fragments arranged by size:
1813 1525 688 631 605 122 109 107
71 39 9
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2568 2747 3780 4936 1790
Size: 778 179 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 778 179
BmgBI CAC'GTC
Cuts at: 1318 2167 2419 1318
Size: 849 252 4618
Fragments arranged by size:
4618 849 252
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4408 442
Size: 416 53 83 399 24 2033 958 1753
Fragments arranged by size:
2033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2094 1056
Size: 1038 4681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 4379 1239
Size: 989 2151 2579
Fragments arranged by size:
2579 2151 989
Bpu10I CC'TnA_GC
Cuts at: 2436 2436
Size: 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2976 4382 882
Size: 617 1477 1406 2219
Fragments arranged by size:
2219 1477 1406 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2432 2481 3526 3908
Size: 53 83 186 936 899 49 1045 382
Cuts at: 3908 4543 275
Size: 635 1451
Fragments arranged by size:
1451 1045 936 899 635 382 186 83
53 49
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5494
Fragments arranged by size:
5494 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 1186
Size: 1195 4524
BseYI C'CCAG_C
Cuts at: 2793 4922 5418 2793
Size: 2129 496 3094
Fragments arranged by size:
3094 2129 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2662 1163
Size: 573 926 4220
Fragments arranged by size:
4220 926 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2355 2364 2414 3169 3930 4958
Size: 275 186 354 9 50 755 761 1028
Cuts at: 4958 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 755 354 275 186 50
9
BsiHKAI G_wGCw'C
Cuts at: 2424 3780 3865 4936 2424
Size: 1356 85 1071 3207
Fragments arranged by size:
3207 1356 1071 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2424 2568 2706 2747 3038 3780 3865
Size: 399 634 144 138 41 291 742 85
Cuts at: 3865 4936 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 634 399 291 144 138
85 41
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 3074 844
Size: 1214 1016 3489
Fragments arranged by size:
3489 1214 1016
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2521 4214 4388 65
Size: 1183 1273 1693 174 1396
Fragments arranged by size:
1693 1396 1273 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2409 2440 2925 4363 4550 1700
Size: 110 599 31 485 1438 187 2869
Fragments arranged by size:
2869 1438 599 485 187 110 31
BsrGI T'GTAC_A
Cuts at: 96 2871 96
Size: 2775 2944
BssHII G'CGCG_C
Cuts at: 1925 2212 1925
Size: 287 5432
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2676 794
Size: 1882 3837
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2835 3049 3416 3801 3818 5270 5356 5368
Size: 863 214 367 385 17 1452 86 12
Cuts at: 5368 5714 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 863 385 367 346 214 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2230 2355 2400 2475 513
Size: 552 1165 125 45 75 3757
Fragments arranged by size:
3757 1165 552 125 75 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2930 4726 809
Size: 507 1614 1796 1802
Fragments arranged by size:
1802 1796 1614 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2222 2352
Size: 54 1425 50 275 18 196 196 130
Cuts at: 2352 2361 2373 2411 3166 4055 5684 8
Size: 9 12 38 755 889 1629 43
Fragments arranged by size:
1629 1425 889 755 275 196 196 130
54 50 43 38 18 12 9
EagI C'GGCC_G
Cuts at: 1537 1812 2352 2361 2411 3166 1537
Size: 275 540 9 50 755 4090
Fragments arranged by size:
4090 755 540 275 50 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2152 2429 2765 3649 1353
Size: 799 277 336 884 3423
Fragments arranged by size:
3423 884 799 336 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 3782 4379 5166 5434 1239
Size: 393 596 1554 597 787 268 1524
Fragments arranged by size:
1554 1524 787 597 596 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2565 2743 2744 2565
Size: 178 1 5540
Fragments arranged by size:
5540 178 1
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2435 2484 2561 2815 4866 1678
Size: 757 49 77 254 2051 2531
Fragments arranged by size:
2531 2051 757 254 77 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2883 2915 4360 4392 4434
Size: 32 135 32 1311 32 1445 32 42
Cuts at: 4434 4466 1373
Size: 32 2626
Fragments arranged by size:
2626 1445 1311 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KasI G'GCGC_C
Cuts at: 2431 2480 2431
Size: 49 5670
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 2514 4833 5017 1305
Size: 870 339 2319 184 2007
Fragments arranged by size:
2319 2007 870 339 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3897
Fragments arranged by size:
3897 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3677 4036 4195 518
Size: 648 2511 359 159 2042
Fragments arranged by size:
2511 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2232 2271 2357 2402 2477 2727 2797 3135
Size: 626 39 86 45 75 250 70 338
Cuts at: 3135 3812 4960 5205 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 626 338 250 245 86
75 70 45 39
NaeI GCC'GGC
Cuts at: 2411 2411
Size: 5719
NarI GG'CG_CC
Cuts at: 2432 2481 2432
Size: 49 5670
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5167
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NgoMIV G'CCGG_C
Cuts at: 2409 2409
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 2090 1052
Size: 1038 4681
NotI GC'GGCC_GC
Cuts at: 2352 3166 2352
Size: 814 4905
NspI r_CATG'y
Cuts at: 1132 1229 2047 3077 4622 1132
Size: 97 818 1030 1545 2229
Fragments arranged by size:
2229 1545 1030 818 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PfoI T'CCnGG_A
Cuts at: 2801 2801
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2564 2743 2564
Size: 179 5540
PstI C_TGCA'G
Cuts at: 830 2994 3071 830
Size: 2164 77 3478
Fragments arranged by size:
3478 2164 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
SacII CC_GC'GG
Cuts at: 2233 2358 2403 2478 2233
Size: 125 45 75 5474
Fragments arranged by size:
5474 125 75 45
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2429 2765 2429
Size: 336 5383
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 2497 2990 3067 4202 4883 5074 826
Size: 219 1452 493 77 1135 681 191 1471
Fragments arranged by size:
1471 1452 1135 681 493 219 191 77
SfiI GGCCn_nnn'nGGCC
Cuts at: 2360 2360
Size: 5719
SfoI GGC'GCC
Cuts at: 2433 2482 2433
Size: 49 5670
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 2563 2751 3046 1872
Size: 691 188 295 4545
Fragments arranged by size:
4545 691 295 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3823 4724 4986 820
Size: 197 926 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SrfI GCCC'GGGC
Cuts at: 2563 2563
Size: 5719
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2682 2706 3158 513
Size: 552 1617 24 452 3074
Fragments arranged by size:
3074 1617 552 452 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3746 3763 3916 4101 4546 325
Size: 1255 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2871 3965 5677
Size: 274 80 33 51 1443 894 1094 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1712 1443 1094 894 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 2561 2749 3044 1870
Size: 691 188 295 4545
Fragments arranged by size:
4545 691 295 188
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI
SwaI XcmI