Restriction map for
pFN21AB9703
(Circular) (Six-base) MAPSORT of: pFN21AB9703.seq Check: 470 from: 1 to: 5689
pFN21AB9703 5689 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AfeI AGC'GCT
Cuts at: 2244 2244
Size: 5689
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2494 3432 5004 2494
Size: 938 1572 3179
Fragments arranged by size:
3179 1572 938
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2260 2405 2743 3014 1870
Size: 73 90 227 145 338 271 4545
Fragments arranged by size:
4545 338 271 227 145 90 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 2454 2724 2783 3010
Size: 325 217 627 533 134 270 59 227
Cuts at: 3010 4465 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 533 325 270 227 217
134 59
BanII G_rGCy'C
Cuts at: 1391 2658 3008 1391
Size: 1267 350 4072
Fragments arranged by size:
4072 1267 350
BbeI G_GCGC'C
Cuts at: 2324 2458 2728 2324
Size: 134 270 5285
Fragments arranged by size:
5285 270 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 928
Size: 971 189 4529
Fragments arranged by size:
4529 971 189
BbvCI CC'TCA_GC
Cuts at: 2648 2648
Size: 5689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2390 2781 4300
Size: 107 122 71 631 605 718 391 1519
Cuts at: 4300 136
Size: 1525
Fragments arranged by size:
1525 1519 718 631 605 391 122 107
71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2786 3750 4906 1790
Size: 996 964 1156 2573
Fragments arranged by size:
2573 1156 996 964
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2243 1056
Size: 1187 4502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2132 4349 1239
Size: 803 90 2217 2579
Fragments arranged by size:
2579 2217 803 90
Bpu10I CC'TnA_GC
Cuts at: 2162 2426 2648 2162
Size: 264 222 5203
Fragments arranged by size:
5203 264 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3814 4679 4977 2855
Size: 959 865 298 3567
Fragments arranged by size:
3567 959 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2321 2455 2547
Size: 53 83 186 936 513 275 134 92
Cuts at: 2547 2725 3496 3878 4513 275
Size: 178 771 382 635 1451
Fragments arranged by size:
1451 936 771 635 513 382 275 186
178 134 92 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5689
BseYI C'CCAG_C
Cuts at: 2801 4892 5388 2801
Size: 2091 496 3102
Fragments arranged by size:
3102 2091 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2681 1163
Size: 573 945 4171
Fragments arranged by size:
4171 945 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2232 3139 3900 4928 1540
Size: 275 186 231 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 231 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2658 3750 3835 4906 2028
Size: 630 1092 85 1071 2811
Fragments arranged by size:
2811 1092 1071 630 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
BsmBI CGTCTCn'nnnn_
Cuts at: 2306 2306
Size: 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2658 2786 3008 3750 3835 4906
Size: 399 238 630 128 222 742 85 1071
Cuts at: 4906 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 630 399 238 222 128
85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2679 3044 844
Size: 1835 365 3489
Fragments arranged by size:
3489 1835 365
BsrBI CCG'CTC
Cuts at: 1394 1823 2430 2435 2521 3576 1394
Size: 429 607 5 86 1055 3507
Fragments arranged by size:
3507 1055 607 429 86 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2457 4333 4520 1700
Size: 110 647 1876 187 2869
Fragments arranged by size:
2869 1876 647 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2177 2362 2657 3749 4761 2177
Size: 185 295 1092 1012 3105
Fragments arranged by size:
3105 1092 1012 295 185
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2494 794
Size: 1700 3989
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2136 487
Size: 683 56 910 4040
Fragments arranged by size:
4040 910 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2505 2625
Size: 54 1425 50 275 18 399 276 120
Cuts at: 2625 3136 4025 5654 8
Size: 511 889 1629 43
Fragments arranged by size:
1629 1425 889 511 399 276 275 120
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2229 3136 1537
Size: 275 417 907 4090
Fragments arranged by size:
4090 907 417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2150 2495 4276 4650 4796 387
Size: 1064 138 561 345 1781 374 146 1280
Fragments arranged by size:
1781 1280 1064 561 374 345 146 138
EcoICRI GAG'CTC
Cuts at: 2656 2656
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2132 3752 4349 5136 5404 1239
Size: 393 410 90 1620 597 787 268 1524
Fragments arranged by size:
1620 1524 787 597 410 393 268 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2603 2603
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2038 2104 2266 2917 2038
Size: 66 162 651 4810
Fragments arranged by size:
4810 651 162 66
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2246 2324 2458 2599 2728 4836 1678
Size: 568 78 134 141 129 2108 2531
Fragments arranged by size:
2531 2108 568 141 134 129 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2829 2861 2969 3001 4330
Size: 32 135 32 1257 32 108 32 1329
Cuts at: 4330 4362 4404 4436 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 1257 135 108 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2320 2454 2724 2320
Size: 134 270 5285
Fragments arranged by size:
5285 270 134
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 10
Size: 54 1425 343 795 3072
Fragments arranged by size:
3072 1425 795 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3647 4006 4165 518
Size: 648 2481 359 159 2042
Fragments arranged by size:
2481 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2494 3105 3782 4930 5175 1606
Size: 888 611 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 677 611 245
NaeI GCC'GGC
Cuts at: 2459 2459
Size: 5689
NarI GG'CG_CC
Cuts at: 2321 2455 2725 2321
Size: 134 270 5285
Fragments arranged by size:
5285 270 134
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2457 2457
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 2239 1052
Size: 1187 4502
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2533 3047 4592 1132
Size: 97 1304 514 1545 2229
Fragments arranged by size:
2229 1545 1304 514 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2904 1108
Size: 1796 3893
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 3758 3790 5271 5303 2141
Size: 32 1585 32 1481 32 2527
Fragments arranged by size:
2527 1585 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2917 2104
Size: 813 4876
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2217 2490 3041 830
Size: 1387 273 551 3478
Fragments arranged by size:
3478 1387 551 273
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2494 2494
Size: 5689
SacI G_AGCT'C
Cuts at: 2658 2658
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SanDI GG'GwC_CC
Cuts at: 2104 2104
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 2183 2397 3937 5649 2183
Size: 214 1540 1712 2223
Fragments arranged by size:
2223 1712 1540 214
SfcI C'TryA_G
Cuts at: 826 1045 2213 2486 3037 4172 4853 5044 826
Size: 219 1168 273 551 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 681 551 273 219 191
SfoI GGC'GCC
Cuts at: 2322 2456 2726 2322
Size: 134 270 5285
Fragments arranged by size:
5285 270 134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 2457 1700
Size: 757 4932
SmaI CCC'GGG
Cuts at: 1872 2035 2407 2745 3016 1872
Size: 163 372 338 271 4545
Fragments arranged by size:
4545 372 338 271 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3793 4694 4956 820
Size: 197 926 927 923 901 262 1553
Fragments arranged by size:
1553 927 926 923 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 2533 3047 1132
Size: 1401 514 3774
Fragments arranged by size:
3774 1401 514
SrfI GCCC'GGGC
Cuts at: 2745 2745
Size: 5689
SspI AAT'ATT
Cuts at: 5 52 2884 3613 5
Size: 47 2832 729 2081
Fragments arranged by size:
2832 2081 729 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2604 3128 513
Size: 552 939 600 524 3074
Fragments arranged by size:
3074 939 600 552 524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2395 3935
Size: 274 80 33 51 1443 204 214 1540
Cuts at: 3935 5647 96
Size: 1712 138
Fragments arranged by size:
1712 1540 1443 274 214 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2033 2405 2743 3014 1870
Size: 163 372 338 271 4545
Fragments arranged by size:
4545 372 338 271 163
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SwaI XcmI