Restriction map for
pFN21AB9702
(Circular) (Six-base) MAPSORT of: pFN21AB9702.seq Check: 3001 from: 1 to: 5686
pFN21AB9702 5686 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2244 2244
Size: 5686
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 2679 3007 2679
Size: 328 5358
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3749 5133 5401 1632
Size: 2117 1384 268 1917
Fragments arranged by size:
2117 1917 1384 268
AfeI AGC'GCT
Cuts at: 2760 2760
Size: 5686
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 2145 2372 2803 3429 5001 2145
Size: 227 431 626 1572 2830
Fragments arranged by size:
2830 1572 626 431 227
ApaLI G'TGCA_C
Cuts at: 2337 3743 4899 2337
Size: 1406 1156 3124
Fragments arranged by size:
3124 1406 1156
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2243 2982 3011 1870
Size: 73 147 153 739 29 4545
Fragments arranged by size:
4545 739 153 147 73 29
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2679 2887 2918 3007 4462
Size: 325 217 627 892 208 31 89 1455
Cuts at: 4462 618
Size: 1842
Fragments arranged by size:
1842 1455 892 627 325 217 208 89
31
BanII G_rGCy'C
Cuts at: 1391 2637 3005 1391
Size: 1246 368 4072
Fragments arranged by size:
4072 1246 368
BbeI G_GCGC'C
Cuts at: 2922 2922
Size: 5686
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2308 2342 2617 2651 3877 3911 1903
Size: 34 371 34 275 34 1226 34 3678
Fragments arranged by size:
3678 1226 371 275 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 2295 1548
Size: 747 4939
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2493 5681 2493
Size: 3188 2498
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 2341 2892 3747 4903 1790
Size: 551 551 855 1156 2573
Fragments arranged by size:
2573 1156 855 551 551
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 3417 4375
Size: 416 53 83 399 24 843 1157 958
Cuts at: 4375 442
Size: 1753
Fragments arranged by size:
1753 1157 958 843 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2148 2482 2488 2603 2747 2858 2897
Size: 893 16 334 6 115 144 111 39
Cuts at: 2897 4346 1239
Size: 1449 2579
Fragments arranged by size:
2579 1449 893 334 144 115 111 39
16 6
Bpu10I CC'TnA_GC
Cuts at: 2373 2612 2373
Size: 239 5447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2872 4349 882
Size: 617 1373 1477 2219
Fragments arranged by size:
2219 1477 1373 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2919 2958 3493 3875
Size: 53 83 186 936 1386 39 535 382
Cuts at: 3875 4510 275
Size: 635 1451
Fragments arranged by size:
1451 1386 936 635 535 382 186 83
53 39
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2275 2492 1186
Size: 1089 217 4380
Fragments arranged by size:
4380 1089 217
BseYI C'CCAG_C
Cuts at: 2852 4889 5385 2852
Size: 2037 496 3153
Fragments arranged by size:
3153 2037 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2639 1163
Size: 573 903 4210
Fragments arranged by size:
4210 903 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2488 2718 3136 3897 4925 1540
Size: 275 186 487 230 418 761 1028 2301
Fragments arranged by size:
2301 1028 761 487 418 275 230 186
BsiHKAI G_wGCw'C
Cuts at: 2341 2637 3747 3832 4903 2341
Size: 296 1110 85 1071 3124
Fragments arranged by size:
3124 1110 1071 296 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2177
Size: 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2341 2637 2892 3005 3747 3832 4903
Size: 399 551 296 255 113 742 85 1071
Cuts at: 4903 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 551 399 296 255 113
85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2656 3041 844
Size: 1400 412 385 3489
Fragments arranged by size:
3489 1400 412 385
BsrBI CCG'CTC
Cuts at: 1394 1823 2599 3573 1394
Size: 429 776 974 3507
Fragments arranged by size:
3507 974 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4181 4355 65
Size: 1183 2933 174 1396
Fragments arranged by size:
2933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2362 2954 4330 4517 1700
Size: 110 552 592 1376 187 2869
Fragments arranged by size:
2869 1376 592 552 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 2546 2856 1925
Size: 621 310 4755
Fragments arranged by size:
4755 621 310
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2145 794
Size: 1351 4335
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2493 3016 3383 3768 3785 5237 5323 5335
Size: 521 523 367 385 17 1452 86 12
Cuts at: 5335 5681 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 523 521 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2195 2403 2845 2905 513
Size: 552 939 191 208 442 60 3294
Fragments arranged by size:
3294 939 552 442 208 191 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 3202 3996 4016 3202
Size: 794 20 4872
Fragments arranged by size:
4872 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2666 4693 809
Size: 507 1350 2027 1802
Fragments arranged by size:
2027 1802 1350 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 2715 2952
Size: 54 1425 50 275 18 564 321 237
Cuts at: 2952 3133 4022 5651 8
Size: 181 889 1629 43
Fragments arranged by size:
1629 1425 889 564 321 275 237 181
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2715 3133 1537
Size: 275 903 418 4090
Fragments arranged by size:
4090 903 418 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2155 3616 1353
Size: 802 1461 3423
Fragments arranged by size:
3423 1461 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2753 4273 4647 4793 387
Size: 1064 138 1164 1520 374 146 1280
Fragments arranged by size:
1520 1280 1164 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2635
Size: 5686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2148 2482 2488 2603 2747 2858
Size: 393 500 16 334 6 115 144 111
Cuts at: 2858 2897 3749 4346 5133 5401 1239
Size: 39 852 597 787 268 1524
Fragments arranged by size:
1524 852 787 597 500 393 334 268
144 115 111 39 16 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2072
Size: 5686
EcoO109I rG'GnC_Cy
Cuts at: 2080 2190 2080
Size: 110 5576
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2762 2834 2922 4833 1678
Size: 1084 72 88 1911 2531
Fragments arranged by size:
2531 1911 1084 88 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373
Size: 32 135 32 2755 32 42 32 2626
Fragments arranged by size:
2755 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KasI G'GCGC_C
Cuts at: 2918 2918
Size: 5686
KpnI G_GTAC'C
Cuts at: 2683 3011 2683
Size: 328 5358
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2706 2835 4800 4984 1305
Size: 1401 129 1965 184 2007
Fragments arranged by size:
2007 1965 1401 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3864
Fragments arranged by size:
3864 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3644 4003 4162 518
Size: 648 2478 359 159 2042
Fragments arranged by size:
2478 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2355 2405 2572 2581 2767 2847 2941 3102
Size: 749 50 167 9 186 80 94 161
Cuts at: 3102 3779 4927 5172 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 245 186 167 161
94 80 50 9
NaeI GCC'GGC
Cuts at: 2364 2956 2364
Size: 592 5094
NarI GG'CG_CC
Cuts at: 2919 2919
Size: 5686
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4195
Fragments arranged by size:
4195 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NgoMIV G'CCGG_C
Cuts at: 2362 2954 2362
Size: 592 5094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2201 2572 1108
Size: 1093 371 4222
Fragments arranged by size:
4222 1093 371
PfoI T'CCnGG_A
Cuts at: 2164 2164
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2190 2080
Size: 110 5576
PshAI GACnn'nnGTC
Cuts at: 1613 2717 1613
Size: 1104 4582
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2241 3038 830
Size: 1411 797 3478
Fragments arranged by size:
3478 1411 797
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2572 2581 2572
Size: 9 5677
SacI G_AGCT'C
Cuts at: 2637 2637
Size: 5686
SacII CC_GC'GG
Cuts at: 2406 2848 2406
Size: 442 5244
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SanDI GG'GwC_CC
Cuts at: 2080 2190 2080
Size: 110 5576
SapI GCTCTTCn'nnn_
Cuts at: 2155 2155
Size: 5686
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SexAI A'CCwGG_T
Cuts at: 2675 2675
Size: 5686
SfcI C'TryA_G
Cuts at: 826 1045 2237 3034 4169 4850 5041 826
Size: 219 1192 797 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 797 681 219 191
SfoI GGC'GCC
Cuts at: 2920 2920
Size: 5686
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 2245 3013 1872
Size: 373 768 4545
Fragments arranged by size:
4545 768 373
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SrfI GCCC'GGGC
Cuts at: 2245 2245
Size: 5686
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2397 3125 513
Size: 552 939 69 324 728 3074
Fragments arranged by size:
3074 939 728 552 324 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 3730 3883 4068 4513 325
Size: 1255 2133 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 2243 3011 1870
Size: 373 768 4545
Fragments arranged by size:
4545 768 373
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SfiI SwaI XcmI