Restriction map for
pFN21AB9701
(Circular) (Six-base) MAPSORT of: pFN21AB9701.seq Check: 331 from: 1 to: 5665
pFN21AB9701 5665 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2169 2169
Size: 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 3728 5112 5380 1632
Size: 953 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1143 953 268
AfeI AGC'GCT
Cuts at: 2654 2654
Size: 5665
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 5633
AlwNI CAG_nnn'CTG
Cuts at: 2161 3408 4980 2161
Size: 1247 1572 2846
Fragments arranged by size:
2846 1572 1247
ApaI G_GGCC'C
Cuts at: 2123 2381 2123
Size: 258 5407
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2110 2333 2351 2932 2990 1870
Size: 73 146 21 223 18 581 58 4545
Fragments arranged by size:
4545 581 223 146 73 58 21 18
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2733 2986 4441 618
Size: 325 217 627 537 409 253 1455 1842
Fragments arranged by size:
1842 1455 627 537 409 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2018 2049 2123 2211 2250 2381 2555 2580
Size: 627 31 74 88 39 131 174 25
Cuts at: 2580 2909 2984 1391
Size: 329 75 4072
Fragments arranged by size:
4072 627 329 174 131 88 75 74
39 31 25
BbeI G_GCGC'C
Cuts at: 2328 2737 2328
Size: 409 5256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2728 2762 3856 3890 1903
Size: 34 791 34 1094 34 3678
Fragments arranged by size:
3678 1094 791 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2559 2568 4276
Size: 107 122 71 631 605 887 9 1708
Cuts at: 4276 136
Size: 1525
Fragments arranged by size:
1708 1525 887 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 2784 5660 2784
Size: 2876 2789
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2381 2454 2531 3726 4882 1790
Size: 333 258 73 77 1195 1156 2573
Fragments arranged by size:
2573 1195 1156 333 258 77 73
BmgBI CAC'GTC
Cuts at: 1318 2407 1318
Size: 1089 4576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2622 3396 4354
Size: 416 53 83 399 24 1205 774 958
Cuts at: 4354 442
Size: 1753
Fragments arranged by size:
1753 1205 958 774 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 4325 1239
Size: 1475 1611 2579
Fragments arranged by size:
2579 1611 1475
Bpu10I CC'TnA_GC
Cuts at: 2649 2649
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 2499 2734 3472
Size: 53 83 186 936 792 174 235 738
Cuts at: 3472 3854 4489 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 792 738 635 382 235 186
174 83 53
BsaWI w'CCGG_w
Cuts at: 2799 4098 4496 4770 4917 5328 2799
Size: 1299 398 274 147 411 3136
Fragments arranged by size:
3136 1299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2687 2717 1178
Size: 30 165 30 1284 30 4126
Fragments arranged by size:
4126 1284 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2947 1186
Size: 895 866 3904
Fragments arranged by size:
3904 895 866
BseYI C'CCAG_C
Cuts at: 2140 2154 2178 2346 2380 2418 2455 2557 4868
Size: 14 24 168 34 38 37 102 2311
Cuts at: 4868 5364 2140
Size: 496 2441
Fragments arranged by size:
2441 2311 496 168 102 38 37 34
24 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5092
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2203 2342 3115 3876 4904 1540
Size: 275 186 202 139 773 761 1028 2301
Fragments arranged by size:
2301 1028 773 761 275 202 186 139
BsiHKAI G_wGCw'C
Cuts at: 2018 2939 3726 3811 4882 2018
Size: 921 787 85 1071 2801
Fragments arranged by size:
2801 1071 921 787 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2049 2123 2211 2250 2381 2454
Size: 399 228 31 74 88 39 131 73
Cuts at: 2454 2531 2555 2580 2909 2939 2984 3726 3811
Size: 77 24 25 329 30 45 742 85
Cuts at: 3811 4882 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 329 228 131 88
85 77 74 73 45 39 31 30
25 24
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2671 3020 844
Size: 1325 502 349 3489
Fragments arranged by size:
3489 1325 502 349
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4160 4334 65
Size: 1183 2912 174 1396
Fragments arranged by size:
2912 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2876 4309 4496 1700
Size: 110 1066 1433 187 2869
Fragments arranged by size:
2869 1433 1066 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2392 794
Size: 1598 4067
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2604 2784 2995 3362 3747 3764 5216 5302
Size: 632 180 211 367 385 17 1452 86
Cuts at: 5302 5314 5660 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 632 385 367 346 211 180
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2231 2289 2317 2465 2580 513
Size: 552 1166 58 28 148 115 3598
Fragments arranged by size:
3598 1166 552 148 115 58 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3975 3995 3181
Size: 794 20 4851
Fragments arranged by size:
4851 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2339 2541
Size: 54 1425 50 275 18 370 139 202
Cuts at: 2541 3112 4001 5630 8
Size: 571 889 1629 43
Fragments arranged by size:
1629 1425 889 571 370 275 202 139
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2200 2339 3112 1537
Size: 275 388 139 773 4090
Fragments arranged by size:
4090 773 388 275 139
EarI CTCTTCn'nnn_
Cuts at: 1353 3595 1353
Size: 2242 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2016 2016
Size: 5665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 2714 3728 4325 5112 5380 1239
Size: 393 953 129 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 953 787 597 393 268 129
EcoO109I rG'GnC_Cy
Cuts at: 2119 2120 2377 2119
Size: 1 257 5407
Fragments arranged by size:
5407 257 1
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 2147 2328 2473 2520 2619 2656 2737 2776
Size: 469 181 145 47 99 37 81 39
Cuts at: 2776 4812 1678
Size: 2036 2531
Fragments arranged by size:
2531 2036 469 181 145 99 81 47
39 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373
Size: 32 135 32 2734 32 42 32 2626
Fragments arranged by size:
2734 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2623 3009 3255 669
Size: 988 281 685 386 246 3079
Fragments arranged by size:
3079 988 685 386 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 2623 3255 2623
Size: 632 5033
KasI G'GCGC_C
Cuts at: 2324 2733 2324
Size: 409 5256
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 4779 4963 1305
Size: 753 2721 184 2007
Fragments arranged by size:
2721 2007 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3843
Fragments arranged by size:
3843 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2843 3623 3982 4141 518
Size: 648 843 834 780 359 159 2042
Fragments arranged by size:
2042 843 834 780 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2167 2233 2291 2319 2459 2467 2533
Size: 479 82 66 58 28 140 8 66
Cuts at: 2533 2557 3081 3758 4906 5151 1606
Size: 24 524 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 524 479 245 140 82
66 66 58 28 24 8
NaeI GCC'GGC
Cuts at: 2878 2878
Size: 5665
NarI GG'CG_CC
Cuts at: 2325 2734 2325
Size: 409 5256
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NgoMIV G'CCGG_C
Cuts at: 2876 2876
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 2339 3112 2339
Size: 773 4892
NspI r_CATG'y
Cuts at: 1132 1229 2399 3023 4568 1132
Size: 97 1170 624 1545 2229
Fragments arranged by size:
2229 1545 1170 624 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2036 2068 2687 2719 3734 3766 5247 5279 2036
Size: 32 619 32 1015 32 1481 32 2422
Fragments arranged by size:
2422 1481 1015 619 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 2634 3235 2634
Size: 601 5064
PspOMI G'GGCC_C
Cuts at: 2119 2377 2119
Size: 258 5407
PstI C_TGCA'G
Cuts at: 830 2172 2415 2439 3017 830
Size: 1342 243 24 578 3478
Fragments arranged by size:
3478 1342 578 243 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 5665
SacI G_AGCT'C
Cuts at: 2018 2018
Size: 5665
SacII CC_GC'GG
Cuts at: 2234 2292 2320 2468 2234
Size: 58 28 148 5431
Fragments arranged by size:
5431 148 58 28
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2168 2411 2435 3013 4148 4829 5020
Size: 219 1123 243 24 578 1135 681 191
Cuts at: 5020 826
Size: 1471
Fragments arranged by size:
1471 1135 1123 681 578 243 219 191
24
SfoI GGC'GCC
Cuts at: 2326 2735 2326
Size: 409 5256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2112 2335 2992 1872
Size: 240 223 657 4545
Fragments arranged by size:
4545 657 240 223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 2399 3023 1132
Size: 1267 624 3774
Fragments arranged by size:
3774 1267 624
SrfI GCCC'GGGC
Cuts at: 2112 2112
Size: 5665
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5665
StyI C'CwwG_G
Cuts at: 513 1065 2373 3104 513
Size: 552 1308 731 3074
Fragments arranged by size:
3074 1308 731 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3692 3709 3862 4047 4492 325
Size: 1255 2112 17 153 185 445 1498
Fragments arranged by size:
2112 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3911 5623 96
Size: 274 80 33 51 1443 1934 1712 138
Fragments arranged by size:
1934 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2935 2967 5625 5657 2935
Size: 32 2658 32 2943
Fragments arranged by size:
2943 2658 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2110 2333 2990 1870
Size: 240 223 657 4545
Fragments arranged by size:
4545 657 240 223
XmnI GAAnn'nnTTC
Cuts at: 2702 3794 2702
Size: 1092 4573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
RsrII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII SanDI SapI SexAI SfiI SwaI XcmI