Restriction map for
pFN21AB9700
(Circular) (Six-base) MAPSORT of: pFN21AB9700.seq Check: 6739 from: 1 to: 5593
pFN21AB9700 5593 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3403 278
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2936 1937
Size: 999 4594
Acc65I G'GTAC_C
Cuts at: 2914 2914
Size: 5593
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2497 2577 2665 2833 3656 5040 5308 1632
Size: 865 80 88 168 823 1384 268 1917
Fragments arranged by size:
1917 1384 865 823 268 168 88 80
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5396
AflIII A'CryG_T
Cuts at: 1203 2741 4492 5469 1203
Size: 1538 1751 977 1327
Fragments arranged by size:
1751 1538 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 4322 4322
Size: 5593
AleI CACnn'nnGTG
Cuts at: 1166 2808 1166
Size: 1642 3951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5593
AlwNI CAG_nnn'CTG
Cuts at: 3336 4908 3336
Size: 1572 4021
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2547 2695 2762 2902 3128 3280 5389 1493
Size: 1054 148 67 140 226 152 2109 1697
Fragments arranged by size:
2109 1697 1054 226 152 148 140 67
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2918 1870
Size: 73 975 4545
Fragments arranged by size:
4545 975 73
BamHI G'GATC_C
Cuts at: 2038 2923 3290 2038
Size: 885 367 4341
Fragments arranged by size:
4341 885 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2379 2914 4369 618
Size: 325 217 627 592 535 1455 1842
Fragments arranged by size:
1842 1455 627 592 535 325 217
BanII G_rGCy'C
Cuts at: 1391 2912 1391
Size: 1521 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3784 3818 1903
Size: 34 1847 34 3678
Fragments arranged by size:
3678 1847 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2185 3485 4695 2185
Size: 1300 1210 3083
Fragments arranged by size:
3083 1300 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4204 136
Size: 107 122 71 631 605 2532 1525
Fragments arranged by size:
2532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2611 5588 2611
Size: 2977 2616
BlpI GC'TnA_GC
Cuts at: 3010 3010
Size: 5593
Bme1580I G_kGCm'C
Cuts at: 1790 3654 4810 1790
Size: 1864 1156 2573
Fragments arranged by size:
2573 1864 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3324 4282 442
Size: 416 53 83 399 24 1907 958 1753
Fragments arranged by size:
1907 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4253 1239
Size: 3014 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3718 4583 4881 3718
Size: 865 298 4430
Fragments arranged by size:
4430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4256 882
Size: 617 2757 2219
Fragments arranged by size:
2757 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3384 493
Size: 711 2180 2702
Fragments arranged by size:
2702 2180 711
BsaBI GATnn'nnATC
Cuts at: 5587 5587
Size: 5593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 3400 3782 4417
Size: 53 83 186 936 759 1108 382 635
Cuts at: 4417 275
Size: 1451
Fragments arranged by size:
1451 1108 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2793 4026 4424 4698 4845 5256 2793
Size: 1233 398 274 147 411 3130
Fragments arranged by size:
3130 1233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5368
Fragments arranged by size:
5368 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5593
BseYI C'CCAG_C
Cuts at: 4796 5292 4796
Size: 496 5097
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5020
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3804 4832 1540
Size: 275 186 1042 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3654 3739 4810 2118
Size: 1536 85 1071 2901
Fragments arranged by size:
2901 1536 1071 85
BsmI GAATG_Cn'
Cuts at: 3104 3197 3104
Size: 93 5500
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2912 3654 3739 4810 1391
Size: 399 328 794 742 85 1071 2174
Fragments arranged by size:
2174 1071 794 742 399 328 85
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 2746 3482 5212 2746
Size: 736 1730 3127
Fragments arranged by size:
3127 1730 736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2948 844
Size: 2104 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3480 1394
Size: 429 1657 3507
Fragments arranged by size:
3507 1657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4088 4262 65
Size: 1183 2840 174 1396
Fragments arranged by size:
2840 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4237 4424 1700
Size: 110 2427 187 2869
Fragments arranged by size:
2869 2427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 3653 4665 3653
Size: 1012 4581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5593
BstBI TT'CG_AA
Cuts at: 4397 4397
Size: 5593
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5593
BstYI r'GATC_y
Cuts at: 1972 2038 2611 2923 3290 3675 3692 5144 5230
Size: 66 573 312 367 385 17 1452 86
Cuts at: 5230 5242 5588 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 573 385 367 346 312 86
66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2162 513
Size: 552 1097 3944
Fragments arranged by size:
3944 1097 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4854
Fragments arranged by size:
4854 683 56
BtsI GCAGTG_nn'
Cuts at: 3109 3903 3923 3109
Size: 794 20 4779
Fragments arranged by size:
4779 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2488 2897 2488
Size: 409 5184
DraIII CAC_nnn'GTG
Cuts at: 2368 2368
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4600 809
Size: 507 3284 1802
Fragments arranged by size:
3284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3040 3929 5558
Size: 54 1425 50 275 18 1210 889 1629
Cuts at: 5558 8
Size: 43
Fragments arranged by size:
1629 1425 1210 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 1537
Size: 275 1228 4090
Fragments arranged by size:
4090 1228 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2553 3523 1353
Size: 798 402 970 3423
Fragments arranged by size:
3423 970 798 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4180 4554 4700 387
Size: 1064 138 2591 374 146 1280
Fragments arranged by size:
2591 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2497 2577 2665 2833 3656 4253 5040
Size: 393 865 80 88 168 823 597 787
Cuts at: 5040 5308 1239
Size: 268 1524
Fragments arranged by size:
1524 865 823 787 597 393 268 168
88 80
EcoO109I rG'GnC_Cy
Cuts at: 2053 2053
Size: 5593
EcoRI G'AATT_C
Cuts at: 2547 2902 3280 2547
Size: 355 378 4860
Fragments arranged by size:
4860 378 355
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 4740 1678
Size: 3062 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 2596 2628 4234
Size: 32 135 32 553 32 439 32 1606
Cuts at: 4234 4266 4308 4340 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1606 553 439 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2579 2937 3183 669
Size: 988 281 641 358 246 3079
Fragments arranged by size:
3079 988 641 358 281 246
HindIII A'AGCT_T
Cuts at: 748 2677 748
Size: 1929 3664
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KpnI G_GTAC'C
Cuts at: 2918 2918
Size: 5593
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4707 4891 1305
Size: 3402 184 2007
Fragments arranged by size:
3402 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3771
Fragments arranged by size:
3771 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2656 2751 2808 3551 3910 4069 518
Size: 648 1490 95 57 743 359 159 2042
Fragments arranged by size:
2042 1490 743 648 359 159 95 57
MspA1I CmG'CkG
Cuts at: 1606 3009 3686 4834 5079 1606
Size: 1403 677 1148 245 2120
Fragments arranged by size:
2120 1403 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2162 513
Size: 552 1097 3944
Fragments arranged by size:
3944 1097 552
NdeI CA'TA_TG
Cuts at: 387 2834 387
Size: 2447 3146
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 3040 3040
Size: 5593
NspI r_CATG'y
Cuts at: 1132 1229 2745 2951 4496 1132
Size: 97 1516 206 1545 2229
Fragments arranged by size:
2229 1545 1516 206 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 2741 4492 2741
Size: 1751 3842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5593
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PstI C_TGCA'G
Cuts at: 830 2945 830
Size: 2115 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SapI GCTCTTCn'nnn_
Cuts at: 2151 2151
Size: 5593
SbfI CC_TGCA'GG
Cuts at: 2945 2945
Size: 5593
ScaI AGT'ACT
Cuts at: 3841 5553 3841
Size: 1712 3881
SexAI A'CCwGG_T
Cuts at: 2889 2889
Size: 5593
SfcI C'TryA_G
Cuts at: 826 1045 2211 2941 4076 4757 4948 826
Size: 219 1166 730 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 730 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2920 1872
Size: 1048 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3697 4598 4860 820
Size: 197 926 1754 901 262 1553
Fragments arranged by size:
1754 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2951 1132
Size: 1819 3774
SspI AAT'ATT
Cuts at: 5 52 3517 5
Size: 47 3465 2081
Fragments arranged by size:
3465 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5593
StyI C'CwwG_G
Cuts at: 513 1065 2162 2638 3032 513
Size: 552 1097 476 394 3074
Fragments arranged by size:
3074 1097 552 476 394
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3620 3637 3790 3975 4420 325
Size: 1255 2040 17 153 185 445 1498
Fragments arranged by size:
2040 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3839 5551 96
Size: 274 80 33 51 1443 1862 1712 138
Fragments arranged by size:
1862 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5553 5585 5553
Size: 32 5561
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5593
XbaI T'CTAG_A
Cuts at: 2143 2929 2143
Size: 786 4807
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2918 1870
Size: 1048 4545
XmnI GAAnn'nnTTC
Cuts at: 2510 2678 3722 2510
Size: 168 1044 4381
Fragments arranged by size:
4381 1044 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3401 276
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI XcmI