Restriction map for
pFN21AB9696
(Circular) (Six-base) MAPSORT of: pFN21AB9696.seq Check: 4138 from: 1 to: 5500
pFN21AB9696 5500 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2584 2584
Size: 5500
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2484 2843 1937
Size: 547 359 4594
Fragments arranged by size:
4594 547 359
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3563 4947 5215 1632
Size: 1931 1384 268 1917
Fragments arranged by size:
1931 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AlwNI CAG_nnn'CTG
Cuts at: 3243 4815 3243
Size: 1572 3928
ApaI G_GGCC'C
Cuts at: 2084 2243 2286 2325 2360 2084
Size: 159 43 39 35 5224
Fragments arranged by size:
5224 159 43 39 35
ApaLI G'TGCA_C
Cuts at: 3557 4713 3557
Size: 1156 4344
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 2329 2351 2359 2825 1870
Size: 73 293 93 22 8 466 4545
Fragments arranged by size:
4545 466 293 93 73 22 8
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2191 2305 2311 2821
Size: 325 217 627 329 75 114 6 510
Cuts at: 2821 4276 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 510 329 325 217 114
75 6
BanII G_rGCy'C
Cuts at: 1391 2084 2243 2286 2325 2360 2583 2819 1391
Size: 693 159 43 39 35 223 236 4072
Fragments arranged by size:
4072 693 236 223 159 43 39 35
BbeI G_GCGC'C
Cuts at: 2120 2195 2315 2120
Size: 75 120 5305
Fragments arranged by size:
5305 120 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 1903
Size: 34 1754 34 3678
Fragments arranged by size:
3678 1754 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 2320 4111
Size: 107 122 71 631 605 517 131 1791
Cuts at: 4111 136
Size: 1525
Fragments arranged by size:
1791 1525 631 605 517 131 122 107
71
BglII A'GATC_T
Cuts at: 2601 5495 2601
Size: 2894 2606
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2243 2286 2325 2360 2366 3561 4717
Size: 294 159 43 39 35 6 1195 1156
Cuts at: 4717 1790
Size: 2573
Fragments arranged by size:
2573 1195 1156 294 159 43 39 35
6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2227 3231 4189
Size: 416 53 83 399 24 810 1004 958
Cuts at: 4189 442
Size: 1753
Fragments arranged by size:
1753 1004 958 810 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2274 2525 2531 4160 1239
Size: 886 149 251 6 1629 2579
Fragments arranged by size:
2579 1629 886 251 149 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 882
Size: 617 2664 2219
Fragments arranged by size:
2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2155 3291 493
Size: 711 951 1136 2702
Fragments arranged by size:
2702 1136 951 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2117 2192 2312 2396
Size: 53 83 186 936 584 75 120 84
Cuts at: 2396 3307 3689 4324 275
Size: 911 382 635 1451
Fragments arranged by size:
1451 936 911 635 584 382 186 120
84 83 75 53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 2752 2782 1178
Size: 30 165 30 1101 30 218 30 3896
Fragments arranged by size:
3896 1101 218 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2755 2801 1186
Size: 1569 46 3885
Fragments arranged by size:
3885 1569 46
BseYI C'CCAG_C
Cuts at: 2053 2263 2707 2738 4703 5199 2053
Size: 210 444 31 1965 496 2354
Fragments arranged by size:
2354 1965 496 444 210 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4927
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2430 2950 3711 4739 1540
Size: 275 186 429 520 761 1028 2301
Fragments arranged by size:
2301 1028 761 520 429 275 186
BsiHKAI G_wGCw'C
Cuts at: 3561 3646 4717 3561
Size: 85 1071 4344
Fragments arranged by size:
4344 1071 85
BsmI GAATG_Cn'
Cuts at: 2276 3011 3104 2276
Size: 735 93 4672
Fragments arranged by size:
4672 735 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2243 2286 2325 2360 2366 2583
Size: 399 294 159 43 39 35 6 217
Cuts at: 2583 2819 3561 3646 4717 1391
Size: 236 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 294 236 217 159
85 43 39 35 6
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2584 2855 844
Size: 1740 271 3489
Fragments arranged by size:
3489 1740 271
BsrBI CCG'CTC
Cuts at: 1394 1823 3387 1394
Size: 429 1564 3507
Fragments arranged by size:
3507 1564 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3995 4169 65
Size: 1183 2747 174 1396
Fragments arranged by size:
2747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2161 2308 2616 4144 4331 1700
Size: 110 351 147 308 1528 187 2869
Fragments arranged by size:
2869 1528 351 308 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5500
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2755 2011
Size: 744 4756
BstYI r'GATC_y
Cuts at: 1972 2601 2830 3197 3582 3599 5051 5137 5149
Size: 629 229 367 385 17 1452 86 12
Cuts at: 5149 5495 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 629 385 367 346 229 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2244 513
Size: 552 1179 3769
Fragments arranged by size:
3769 1179 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3810 3830 3016
Size: 794 20 4686
Fragments arranged by size:
4686 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 809
Size: 507 3191 1802
Fragments arranged by size:
3191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 2700 2757
Size: 54 1425 50 275 18 597 273 57
Cuts at: 2757 2947 3836 5465 8
Size: 190 889 1629 43
Fragments arranged by size:
1629 1425 889 597 275 273 190 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2427 2947 1537
Size: 275 615 520 4090
Fragments arranged by size:
4090 615 520 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 1353
Size: 2077 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2407 2566 4087 4461 4607 387
Size: 1064 138 818 159 1521 374 146 1280
Fragments arranged by size:
1521 1280 1064 818 374 159 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2274 2525 2531 3563 4160 4947
Size: 393 493 149 251 6 1032 597 787
Cuts at: 4947 5215 1239
Size: 268 1524
Fragments arranged by size:
1524 1032 787 597 493 393 268 251
149 6
EcoO109I rG'GnC_Cy
Cuts at: 2240 2283 2356 2728 2240
Size: 43 73 372 5012
Fragments arranged by size:
5012 372 73 43
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2120 2195 2315 4647 1678
Size: 442 75 120 2332 2531
Fragments arranged by size:
2531 2332 442 120 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4141 4173 4215 4247 1373
Size: 32 135 32 2569 32 42 32 2626
Fragments arranged by size:
2626 2569 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 3090 669
Size: 988 281 906 246 3079
Fragments arranged by size:
3079 988 906 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KasI G'GCGC_C
Cuts at: 2116 2191 2311 2116
Size: 75 120 5305
Fragments arranged by size:
5305 120 75
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4614 4798 1305
Size: 3309 184 2007
Fragments arranged by size:
3309 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 10
Size: 54 1425 343 927 2751
Fragments arranged by size:
2751 1425 927 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3458 3817 3976 518
Size: 648 843 1449 359 159 2042
Fragments arranged by size:
2042 1449 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2246 2448 2916 3593 4741 4986 1606
Size: 640 202 468 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 640 468 245 202
NaeI GCC'GGC
Cuts at: 2163 2310 2163
Size: 147 5353
NarI GG'CG_CC
Cuts at: 2117 2192 2312 2117
Size: 75 120 5305
Fragments arranged by size:
5305 120 75
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4948
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NgoMIV G'CCGG_C
Cuts at: 2161 2308 2161
Size: 147 5353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2858 4403 1132
Size: 97 1629 1545 2229
Fragments arranged by size:
2229 1629 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2318 2722 1348
Size: 970 404 4126
Fragments arranged by size:
4126 970 404
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2503 1108
Size: 1395 4105
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PmlI CAC'GTG
Cuts at: 2155 2155
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5500
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PspOMI G'GGCC_C
Cuts at: 2080 2239 2282 2321 2356 2080
Size: 159 43 39 35 5224
Fragments arranged by size:
5224 159 43 39 35
PstI C_TGCA'G
Cuts at: 830 2852 830
Size: 2022 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
SacII CC_GC'GG
Cuts at: 2247 2247
Size: 5500
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SfcI C'TryA_G
Cuts at: 826 1045 2848 3983 4664 4855 826
Size: 219 1803 1135 681 191 1471
Fragments arranged by size:
1803 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2118 2193 2313 2118
Size: 75 120 5305
Fragments arranged by size:
5305 120 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2238 2331 2361 2827 1872
Size: 366 93 30 466 4545
Fragments arranged by size:
4545 466 366 93 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2858 1132
Size: 1726 3774
SrfI GCCC'GGGC
Cuts at: 2238 2331 2361 2238
Size: 93 30 5377
Fragments arranged by size:
5377 93 30
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2208 2463 2939 513
Size: 552 1143 255 476 3074
Fragments arranged by size:
3074 1143 552 476 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3527 3544 3697 3882 4327 325
Size: 1255 1947 17 153 185 445 1498
Fragments arranged by size:
1947 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3746 5458 96
Size: 274 80 33 51 1443 1769 1712 138
Fragments arranged by size:
1769 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2752 2784 5460 5492 2752
Size: 32 2676 32 2760
Fragments arranged by size:
2760 2676 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5500
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2074
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2236 2329 2359 2825 1870
Size: 366 93 30 466 4545
Fragments arranged by size:
4545 466 366 93 30
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SwaI