Restriction map for
pFN21AB9695
(Circular) (Six-base) MAPSORT of: pFN21AB9695.seq Check: 779 from: 1 to: 5497
pFN21AB9695 5497 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2563 2563
Size: 5497
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3560 4944 5212 1632
Size: 1928 1384 268 1917
Fragments arranged by size:
1928 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2468 2495 1184
Size: 1284 27 4186
Fragments arranged by size:
4186 1284 27
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 2483
Size: 32 5465
AlwNI CAG_nnn'CTG
Cuts at: 3240 4812 3240
Size: 1572 3925
ApaI G_GGCC'C
Cuts at: 2214 2214
Size: 5497
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2216 2315 2822 1870
Size: 73 123 150 99 507 4545
Fragments arranged by size:
4545 507 150 123 99 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2138 2657 2818 4273
Size: 325 217 627 275 76 519 161 1455
Cuts at: 4273 618
Size: 1842
Fragments arranged by size:
1842 1455 627 519 325 275 217 161
76
BanII G_rGCy'C
Cuts at: 1391 2214 2816 1391
Size: 823 602 4072
Fragments arranged by size:
4072 823 602
BbeI G_GCGC'C
Cuts at: 2066 2142 2066
Size: 76 5421
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BbvCI CC'TCA_GC
Cuts at: 2710 2710
Size: 5497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2524 2558 3688 3722 1903
Size: 34 50 34 503 34 1130 34 3678
Fragments arranged by size:
3678 1130 503 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2218 4108 136
Size: 107 122 71 631 605 546 1890 1525
Fragments arranged by size:
1890 1525 631 605 546 122 107 71
BglII A'GATC_T
Cuts at: 2580 5492 2580
Size: 2912 2585
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2180 2214 3558 4714 1790
Size: 316 74 34 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 316 74 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4186 442
Size: 416 53 83 399 24 1811 958 1753
Fragments arranged by size:
1811 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 4157 1239
Size: 1271 1647 2579
Fragments arranged by size:
2579 1647 1271
Bpu10I CC'TnA_GC
Cuts at: 2710 2710
Size: 5497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 3622 4487 4785 2225
Size: 1397 865 298 2937
Fragments arranged by size:
2937 1397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 4160 882
Size: 617 633 2028 2219
Fragments arranged by size:
2219 2028 633 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 2139 3304 3686
Size: 53 83 186 936 530 76 1165 382
Cuts at: 3686 4321 275
Size: 635 1451
Fragments arranged by size:
1451 1165 936 635 530 382 186 83
76 53
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2354 2384 2483 2513 2776
Size: 30 165 30 951 30 99 30 263
Cuts at: 2776 2806 1178
Size: 30 3869
Fragments arranged by size:
3869 951 263 165 99 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2698 2749 1186
Size: 1512 51 3934
Fragments arranged by size:
3934 1512 51
BseYI C'CCAG_C
Cuts at: 2159 2438 4700 5196 2159
Size: 279 2262 496 2460
Fragments arranged by size:
2460 2262 496 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 1163
Size: 573 369 4555
Fragments arranged by size:
4555 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2337 2776 2947 3708 4736 1540
Size: 275 186 336 439 171 761 1028 2301
Fragments arranged by size:
2301 1028 761 439 336 275 186 171
BsiHKAI G_wGCw'C
Cuts at: 2723 3558 3643 4714 2723
Size: 835 85 1071 3506
Fragments arranged by size:
3506 1071 835 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2180 2214 2723 2816 3558 3643
Size: 399 316 74 34 509 93 742 85
Cuts at: 3643 4714 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 509 399 316 93 85
74 34
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2563 2852 844
Size: 1719 289 3489
Fragments arranged by size:
3489 1719 289
BsrBI CCG'CTC
Cuts at: 1394 1823 2086 2182 3384 1394
Size: 429 263 96 1202 3507
Fragments arranged by size:
3507 1202 429 263 96
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2660 4141 4328 1700
Size: 110 850 1481 187 2869
Fragments arranged by size:
2869 1481 850 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 2150 1925
Size: 225 5272
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2119 794
Size: 1325 4172
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2580 2779 2827 3194 3579 3596 5048 5134
Size: 608 199 48 367 385 17 1452 86
Cuts at: 5134 5146 5492 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 608 385 367 346 199 86
48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2761 2761
Size: 5497
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4945
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2685 487
Size: 683 56 1459 3299
Fragments arranged by size:
3299 1459 683 56
BtsI GCAGTG_nn'
Cuts at: 2401 3013 3807 3827 2401
Size: 612 794 20 4071
Fragments arranged by size:
4071 794 612 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2620 2655 529
Size: 35 2056 35 3371
Fragments arranged by size:
3371 2056 35 35
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2944 3833
Size: 54 1425 50 275 18 504 610 889
Cuts at: 3833 5462 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 610 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2334 2944 1537
Size: 275 522 610 4090
Fragments arranged by size:
4090 610 522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 3427 1353
Size: 740 1334 3423
Fragments arranged by size:
3423 1334 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 3560 4157 4944 5212 1239
Size: 393 878 1050 597 787 268 1524
Fragments arranged by size:
1524 1050 878 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2211 2211
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 5497
FspI TGC'GCA
Cuts at: 2456 2698 4003 2456
Size: 242 1305 3950
Fragments arranged by size:
3950 1305 242
FspAI rTGC'GCAy
Cuts at: 2456 2698 2456
Size: 242 5255
HaeII r_GCGC'y
Cuts at: 1678 2066 2142 4644 1678
Size: 388 76 2502 2531
Fragments arranged by size:
2531 2502 388 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2440 4138 4170 4212
Size: 32 135 32 836 32 1698 32 42
Cuts at: 4212 4244 1373
Size: 32 2626
Fragments arranged by size:
2626 1698 836 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KasI G'GCGC_C
Cuts at: 2062 2138 2062
Size: 76 5421
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 3455 3814 3973 518
Size: 648 1245 1044 359 159 2042
Fragments arranged by size:
2042 1245 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2203 2342 2380 2434 2479 2913 3590 4738
Size: 597 139 38 54 45 434 677 1148
Cuts at: 4738 4983 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 597 434 245 139 54
45 38
NarI GG'CG_CC
Cuts at: 2063 2139 2063
Size: 76 5421
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4945
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NruI TCG'CGA
Cuts at: 2546 2546
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2287 1348
Size: 939 4558
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2483 2515 3566 3598 5079 5111 2483
Size: 32 1051 32 1481 32 2869
Fragments arranged by size:
2869 1481 1051 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PspOMI G'GGCC_C
Cuts at: 2210 2210
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2849 830
Size: 2019 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
PvuII CAG'CTG
Cuts at: 2203 2434 2479 2203
Size: 231 45 5221
Fragments arranged by size:
5221 231 45
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SapI GCTCTTCn'nnn_
Cuts at: 2093 2093
Size: 5497
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 2273 3745 5457 2273
Size: 1472 1712 2313
Fragments arranged by size:
2313 1712 1472
SfcI C'TryA_G
Cuts at: 826 1045 2845 3980 4661 4852 826
Size: 219 1800 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2218 2218
Size: 5497
SfoI GGC'GCC
Cuts at: 2064 2140 2064
Size: 76 5421
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2218 2317 2824 1872
Size: 346 99 507 4545
Fragments arranged by size:
4545 507 346 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 3601 4502 4764 820
Size: 197 926 297 1361 901 262 1553
Fragments arranged by size:
1553 1361 926 901 297 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2936 513
Size: 552 1871 3074
Fragments arranged by size:
3074 1871 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3524 3541 3694 3879 4324 325
Size: 1255 1944 17 153 185 445 1498
Fragments arranged by size:
1944 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 3743 5455
Size: 274 80 33 51 1443 294 1472 1712
Cuts at: 5455 96
Size: 138
Fragments arranged by size:
1712 1472 1443 294 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2216 2315 2822 1870
Size: 346 99 507 4545
Fragments arranged by size:
4545 507 346 99
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI NaeI
NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SrfI SwaI XcmI