Restriction map for
pFN21AB9688
(Circular) (Six-base) MAPSORT of: pFN21AB9688.seq Check: 5703 from: 1 to: 5371
pFN21AB9688 5371 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3181 278
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 2714 1937
Size: 250 527 4594
Fragments arranged by size:
4594 527 250
Acc65I G'GTAC_C
Cuts at: 2692 2692
Size: 5371
AclI AA'CG_TT
Cuts at: 1563 3498 3871 1563
Size: 1935 373 3063
Fragments arranged by size:
3063 1935 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3434 4818 5086 1632
Size: 1802 1384 268 1917
Fragments arranged by size:
1917 1802 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2137 820
Size: 197 1120 4054
Fragments arranged by size:
4054 1120 197
AflIII A'CryG_T
Cuts at: 1203 4270 5247 1203
Size: 3067 977 1327
Fragments arranged by size:
3067 1327 977
AgeI A'CCGG_T
Cuts at: 4202 4202
Size: 5371
AhdI GACnn_n'nnGTC
Cuts at: 4100 4100
Size: 5371
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5371
AlwNI CAG_nnn'CTG
Cuts at: 2118 2506 3114 4686 2118
Size: 388 608 1572 2803
Fragments arranged by size:
2803 1572 608 388
ApaLI G'TGCA_C
Cuts at: 3428 4584 3428
Size: 1156 4215
ApoI r'AATT_y
Cuts at: 1493 2522 2680 2906 3058 5167 1493
Size: 1029 158 226 152 2109 1697
Fragments arranged by size:
2109 1697 1029 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 3925 160
Size: 872 2893 1606
Fragments arranged by size:
2893 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2291 2513 2696 1870
Size: 73 262 86 222 183 4545
Fragments arranged by size:
4545 262 222 183 86 73
BamHI G'GATC_C
Cuts at: 2701 3068 2701
Size: 367 5004
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2692 4147 618
Size: 325 217 627 905 1455 1842
Fragments arranged by size:
1842 1455 905 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2045 2212 2690 1391
Size: 621 33 167 478 4072
Fragments arranged by size:
4072 621 478 167 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2548 2582 3562 3596 1903
Size: 34 611 34 980 34 3678
Fragments arranged by size:
3678 980 611 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3263 4473 3263
Size: 1210 4161
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3982 136
Size: 107 122 71 631 605 2310 1525
Fragments arranged by size:
2310 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2604 5366 2604
Size: 2762 2609
BlpI GC'TnA_GC
Cuts at: 2788 2788
Size: 5371
Bme1580I G_kGCm'C
Cuts at: 1790 3432 4588 1790
Size: 1642 1156 2573
Fragments arranged by size:
2573 1642 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3102 4060 442
Size: 416 53 83 399 24 1685 958 1753
Fragments arranged by size:
1753 1685 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 4031 1239
Size: 1109 1683 2579
Fragments arranged by size:
2579 1683 1109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3496 4361 4659 3496
Size: 865 298 4208
Fragments arranged by size:
4208 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4034 882
Size: 617 2535 2219
Fragments arranged by size:
2535 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2017 3162 493
Size: 711 813 1145 2702
Fragments arranged by size:
2702 1145 813 711
BsaBI GATnn'nnATC
Cuts at: 2603 5365 2603
Size: 2762 2609
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3178 3560 4195 275
Size: 53 83 186 936 1645 382 635 1451
Fragments arranged by size:
1645 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3804 4202 4476 4623 5034 3804
Size: 398 274 147 411 4141
Fragments arranged by size:
4141 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5146
Fragments arranged by size:
5146 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2271 2345 2378 1186
Size: 1085 74 33 4179
Fragments arranged by size:
4179 1085 74 33
BseYI C'CCAG_C
Cuts at: 4574 5070 4574
Size: 496 4875
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2429 1163
Size: 573 387 306 4105
Fragments arranged by size:
4105 573 387 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2221 2244 2821 3582 4610 1540
Size: 275 186 220 23 577 761 1028 2301
Fragments arranged by size:
2301 1028 761 577 275 220 186 23
BsiHKAI G_wGCw'C
Cuts at: 2012 3432 3517 4588 2012
Size: 1420 85 1071 2795
Fragments arranged by size:
2795 1420 1071 85
BsmI GAATG_Cn'
Cuts at: 2882 2975 2882
Size: 93 5278
BsmBI CGTCTCn'nnnn_
Cuts at: 2262 2453 2262
Size: 191 5180
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2045 2212 2690 3432 3517 4588
Size: 399 222 33 167 478 742 85 1071
Cuts at: 4588 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 478 399 222 167 85
33
BspEI T'CCGG_A
Cuts at: 5034 5034
Size: 5371
BspHI T'CATG_A
Cuts at: 3260 4990 3260
Size: 1730 3641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 844
Size: 1882 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2246 3258 1394
Size: 429 423 1012 3507
Fragments arranged by size:
3507 1012 429 423
BsrDI GCAATG_nn'
Cuts at: 65 1248 3866 4040 65
Size: 1183 2618 174 1396
Fragments arranged by size:
2618 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2407 4015 4202 1700
Size: 110 597 1608 187 2869
Fragments arranged by size:
2869 1608 597 187 110
BsrGI T'GTAC_A
Cuts at: 96 2298 96
Size: 2202 3169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5371
BssSI C'ACGA_G
Cuts at: 2456 3431 4443 2456
Size: 975 1012 3384
Fragments arranged by size:
3384 1012 975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5371
BstBI TT'CG_AA
Cuts at: 4175 4175
Size: 5371
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5371
BstYI r'GATC_y
Cuts at: 1972 2604 2701 3068 3453 3470 4922 5008 5020
Size: 632 97 367 385 17 1452 86 12
Cuts at: 5020 5366 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 632 385 367 346 97 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2179 2238 2429 513
Size: 552 1114 59 191 3455
Fragments arranged by size:
3455 1114 552 191 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4632
Fragments arranged by size:
4632 683 56
BtsI GCAGTG_nn'
Cuts at: 2353 2887 3681 3701 2353
Size: 534 794 20 4023
Fragments arranged by size:
4023 794 534 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5336
DraI TTT'AAA
Cuts at: 2675 2675
Size: 5371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4378 809
Size: 507 3062 1802
Fragments arranged by size:
3062 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2818 3707
Size: 54 1425 50 275 18 411 577 889
Cuts at: 3707 5336 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 577 411 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2241 2818 1537
Size: 275 429 577 4090
Fragments arranged by size:
4090 577 429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3301 1353
Size: 1948 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2142 3958 4332 4478 387
Size: 1064 138 553 1816 374 146 1280
Fragments arranged by size:
1816 1280 1064 553 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 3434 4031 4818 5086 1239
Size: 393 716 1086 597 787 268 1524
Fragments arranged by size:
1524 1086 787 716 597 393 268
EcoRI G'AATT_C
Cuts at: 2522 2680 3058 2522
Size: 158 378 4835
Fragments arranged by size:
4835 378 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2163 2684 2163
Size: 521 4850
FspI TGC'GCA
Cuts at: 3877 3877
Size: 5371
HaeII r_GCGC'y
Cuts at: 1678 2238 2327 4518 1678
Size: 560 89 2191 2531
Fragments arranged by size:
2531 2191 560 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2287 2319 4012 4044 4086
Size: 32 135 32 715 32 1693 32 42
Cuts at: 4086 4118 1373
Size: 32 2626
Fragments arranged by size:
2626 1693 715 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2715 2961 669
Size: 988 281 777 246 3079
Fragments arranged by size:
3079 988 777 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5371
HpaI GTT'AAC
Cuts at: 2961 2961
Size: 5371
KpnI G_GTAC'C
Cuts at: 2696 2696
Size: 5371
MfeI C'AATT_G
Cuts at: 2970 2970
Size: 5371
MluI A'CGCG_T
Cuts at: 5247 5247
Size: 5371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 4485 4669 1305
Size: 888 2292 184 2007
Fragments arranged by size:
2292 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3549
Fragments arranged by size:
3549 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3329 3688 3847 518
Size: 648 2163 359 159 2042
Fragments arranged by size:
2163 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2240 2787 3464 4612 4857 1606
Size: 634 547 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 634 547 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4819
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5371
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5371
NotI GC'GGCC_GC
Cuts at: 2241 2818 2241
Size: 577 4794
NspI r_CATG'y
Cuts at: 1132 1229 2068 2729 4274 1132
Size: 97 839 661 1545 2229
Fragments arranged by size:
2229 1545 839 661 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5371
PciI A'CATG_T
Cuts at: 4270 4270
Size: 5371
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2506 1108
Size: 1398 3973
PfoI T'CCnGG_A
Cuts at: 2248 2248
Size: 5371
PmeI GTTT'AAAC
Cuts at: 2675 2675
Size: 5371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 4953 4985 3440
Size: 32 1481 32 3826
Fragments arranged by size:
3826 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5371
PsiI TTA'TAA
Cuts at: 2941 2941
Size: 5371
PstI C_TGCA'G
Cuts at: 830 2234 2723 830
Size: 1404 489 3478
Fragments arranged by size:
3478 1404 489
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5371
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5371
SacII CC_GC'GG
Cuts at: 2241 2241
Size: 5371
SalI G'TCGA_C
Cuts at: 1936 2713 1936
Size: 777 4594
SbfI CC_TGCA'GG
Cuts at: 2723 2723
Size: 5371
ScaI AGT'ACT
Cuts at: 3619 5331 3619
Size: 1712 3659
SfcI C'TryA_G
Cuts at: 826 1045 2230 2719 3854 4535 4726 826
Size: 219 1185 489 1135 681 191 1471
Fragments arranged by size:
1471 1185 1135 681 489 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5371
SmaI CCC'GGG
Cuts at: 1872 2207 2293 2698 1872
Size: 335 86 405 4545
Fragments arranged by size:
4545 405 335 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2137 2513 3475 4376 4638 820
Size: 197 926 194 376 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 376 262 197 194
SnaBI TAC'GTA
Cuts at: 493 2017 3162 493
Size: 1524 1145 2702
Fragments arranged by size:
2702 1524 1145
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5371
SphI G_CATG'C
Cuts at: 1132 2729 1132
Size: 1597 3774
SspI AAT'ATT
Cuts at: 5 52 3295 5
Size: 47 3243 2081
Fragments arranged by size:
3243 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5371
StyI C'CwwG_G
Cuts at: 513 1065 2810 513
Size: 552 1745 3074
Fragments arranged by size:
3074 1745 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3398 3415 3568 3753 4198 325
Size: 1255 1818 17 153 185 445 1498
Fragments arranged by size:
1818 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 3617 5329
Size: 274 80 33 51 1443 321 1319 1712
Cuts at: 5329 96
Size: 138
Fragments arranged by size:
1712 1443 1319 321 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5331 5363 5331
Size: 32 5339
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5371
XbaI T'CTAG_A
Cuts at: 2707 2707
Size: 5371
XhoI C'TCGA_G
Cuts at: 1943 2513 1943
Size: 570 4801
XmaI C'CCGG_G
Cuts at: 1870 2205 2291 2696 1870
Size: 335 86 405 4545
Fragments arranged by size:
4545 405 335 86
XmnI GAAnn'nnTTC
Cuts at: 2522 3500 2522
Size: 978 4393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3179 276
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI