Restriction map for
pFN21AB9669
(Circular) (Six-base) MAPSORT of: pFN21AB9669.seq Check: 4197 from: 1 to: 6172
pFN21AB9669 6172 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2150 3465 2150
Size: 1315 4857
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 1937
Size: 1578 4594
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 2331 2572 2740 2824 2992 3076 3244
Size: 626 73 241 168 84 168 84 168
Cuts at: 3244 3412 4235 5619 5887 1632
Size: 168 823 1384 268 1917
Fragments arranged by size:
1917 1384 823 626 268 241 168 168
168 168 84 84 73
AfeI AGC'GCT
Cuts at: 2513 3041 2513
Size: 528 5644
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 2655 2730 2814 2982 3234 5071 6048 1203
Size: 1452 75 84 168 252 1837 977 1327
Fragments arranged by size:
1837 1452 1327 977 252 168 84 75
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 3915 5487 3915
Size: 1572 4600
ApaLI G'TGCA_C
Cuts at: 2540 2612 2780 3200 3212 3368 4229 5385 2540
Size: 72 168 420 12 156 861 1156 3327
Fragments arranged by size:
3327 1156 861 420 168 156 72 12
ApoI r'AATT_y
Cuts at: 1493 2184 3481 3707 3859 5968 1493
Size: 691 1297 226 152 2109 1697
Fragments arranged by size:
2109 1697 1297 691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2822 3497 1870
Size: 73 879 675 4545
Fragments arranged by size:
4545 879 675 73
BamHI G'GATC_C
Cuts at: 2017 2454 2706 3294 3378 3502 3869 2017
Size: 437 252 588 84 124 367 4320
Fragments arranged by size:
4320 588 437 367 252 124 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 3493 4948 618
Size: 325 217 627 303 1403 1455 1842
Fragments arranged by size:
1842 1455 1403 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2216 2936 3020 3491 1391
Size: 825 720 84 471 4072
Fragments arranged by size:
4072 825 720 471 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 2684 3428 928
Size: 971 147 638 744 3672
Fragments arranged by size:
3672 971 744 638 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 1903
Size: 34 2426 34 3678
Fragments arranged by size:
3678 2426 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2143 4783 136
Size: 107 122 71 631 605 471 2640 1525
Fragments arranged by size:
2640 1525 631 605 471 122 107 71
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 3589 3589
Size: 6172
Bme1580I G_kGCm'C
Cuts at: 1790 2544 2616 2784 2822 3204 3216 3372 4233
Size: 754 72 168 38 382 12 156 861
Cuts at: 4233 5389 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 861 754 382 168 156 72
38 12
BmgBI CAC'GTC
Cuts at: 1318 2656 1318
Size: 1338 4834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3903 4861 442
Size: 416 53 83 399 24 2486 958 1753
Fragments arranged by size:
2486 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2323 4832 1239
Size: 1084 2509 2579
Fragments arranged by size:
2579 2509 1084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4835 882
Size: 617 3336 2219
Fragments arranged by size:
3336 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2815 2983 3235 3963 493
Size: 711 1611 168 252 728 2702
Fragments arranged by size:
2702 1611 728 711 252 168
BsaBI GATnn'nnATC
Cuts at: 6166 6166
Size: 6172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3979 4361 4996 275
Size: 53 83 186 936 2446 382 635 1451
Fragments arranged by size:
2446 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2673 4605 5003 5277 5424 5835 2673
Size: 1932 398 274 147 411 3010
Fragments arranged by size:
3010 1932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 2795 2825 2879
Size: 30 165 30 630 30 732 30 54
Cuts at: 2879 2909 2918 2948 1178
Size: 30 9 30 4402
Fragments arranged by size:
4402 732 630 165 54 30 30 30
30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2926 3094 3346 1186
Size: 1207 533 168 252 4012
Fragments arranged by size:
4012 1207 533 252 168
BseYI C'CCAG_C
Cuts at: 2694 5375 5871 2694
Size: 2681 496 2995
Fragments arranged by size:
2995 2681 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2184 2933 3091 1163
Size: 573 448 749 158 4244
Fragments arranged by size:
4244 749 573 448 158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3622 4383 5411 1540
Size: 275 186 1621 761 1028 2301
Fragments arranged by size:
2301 1621 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2544 2616 2784 2868 2936 3020 3204 3216 3288
Size: 72 168 84 68 84 184 12 72
Cuts at: 3288 3372 4233 4318 5389 2544
Size: 84 861 85 1071 3327
Fragments arranged by size:
3327 1071 861 184 168 85 84 84
84 72 72 68 12
BsiWI C'GTAC_G
Cuts at: 2811 2895 2811
Size: 84 6088
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2544 2616 2784 2822 2868 2936
Size: 399 426 328 72 168 38 46 68
Cuts at: 2936 3020 3204 3216 3288 3372 3491 4233 4318
Size: 84 184 12 72 84 119 742 85
Cuts at: 4318 5389 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 328 184 168
119 85 84 84 72 72 68 46
38 12
BspEI T'CCGG_A
Cuts at: 2673 5835 2673
Size: 3162 3010
BspHI T'CATG_A
Cuts at: 2230 4061 5791 2230
Size: 1831 1730 2611
Fragments arranged by size:
2611 1831 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2150 3465 3527 844
Size: 1306 1315 62 3489
Fragments arranged by size:
3489 1315 1306 62
BsrBI CCG'CTC
Cuts at: 1394 1823 2704 4059 1394
Size: 429 881 1355 3507
Fragments arranged by size:
3507 1355 881 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2966 3134 3302 4816 5003 1700
Size: 110 1156 168 168 1514 187 2869
Fragments arranged by size:
2869 1514 1156 187 168 168 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6172
BssSI C'ACGA_G
Cuts at: 2080 2699 4232 5244 2080
Size: 619 1533 1012 3008
Fragments arranged by size:
3008 1533 1012 619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2910 3078 794
Size: 2116 168 3888
Fragments arranged by size:
3888 2116 168
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstXI CCAn_nnnn'nTGG
Cuts at: 2133 2133
Size: 6172
BstYI r'GATC_y
Cuts at: 1972 2017 2454 2706 3294 3378 3502 3869 4254
Size: 45 437 252 588 84 124 367 385
Cuts at: 4254 4271 5723 5809 5821 6167 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 588 437 385 367 346 252
124 86 84 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2067 2147 2276 513
Size: 552 1002 80 129 4409
Fragments arranged by size:
4409 1002 552 129 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5433
Fragments arranged by size:
5433 683 56
BtsI GCAGTG_nn'
Cuts at: 3688 4482 4502 3688
Size: 794 20 5358
Fragments arranged by size:
5358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5179 809
Size: 507 3863 1802
Fragments arranged by size:
3863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2135 3325 3619
Size: 54 1425 50 275 18 305 1190 294
Cuts at: 3619 4508 6137 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1190 889 305 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3619 1537
Size: 275 1807 4090
Fragments arranged by size:
4090 1807 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4102 1353
Size: 2749 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2934 3018 2934
Size: 84 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2323 2331 2572 2740 2824 2992
Size: 393 626 65 8 241 168 84 168
Cuts at: 2992 3076 3244 3412 4235 4832 5619 5887 1239
Size: 84 168 168 823 597 787 268 1524
Fragments arranged by size:
1524 823 787 626 597 393 268 241
168 168 168 168 84 84 65 8
EcoO109I rG'GnC_Cy
Cuts at: 2075 2099 2304 2075
Size: 24 205 5943
Fragments arranged by size:
5943 205 24
EcoRI G'AATT_C
Cuts at: 2184 3481 3859 2184
Size: 1297 378 4497
Fragments arranged by size:
4497 1297 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 6172
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2515 3043 3187 3273 5319 1678
Size: 837 528 144 86 2046 2531
Fragments arranged by size:
2531 2046 837 528 144 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2501 2533 2671
Size: 32 135 32 461 32 436 32 138
Cuts at: 2671 2703 4813 4845 4887 4919 1373
Size: 32 2110 32 42 32 2626
Fragments arranged by size:
2626 2110 461 436 138 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3516 3762 669
Size: 988 281 1578 246 3079
Fragments arranged by size:
3079 1578 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 2730 6048 2730
Size: 3318 2854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1995 5286 5470 1305
Size: 690 3291 184 2007
Fragments arranged by size:
3291 2007 690 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2137 10
Size: 54 1425 343 305 4045
Fragments arranged by size:
4045 1425 343 305 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3053 4130 4489 4648 518
Size: 648 1887 1077 359 159 2042
Fragments arranged by size:
2042 1887 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2133 2149 2536 3292 3376 3588 4265 5413
Size: 527 16 387 756 84 212 677 1148
Cuts at: 5413 5658 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 756 677 527 387 245 212
84 16
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5620
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 2229 2770 2854 3530 5075 1132
Size: 97 1000 541 84 676 1545 2229
Fragments arranged by size:
2229 1545 1000 676 541 97 84
PasI CC'CwG_GG
Cuts at: 1348 2191 1348
Size: 843 5329
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6172
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2075 2099 2075
Size: 24 6148
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PstI C_TGCA'G
Cuts at: 830 2402 3524 830
Size: 1572 1122 3478
Fragments arranged by size:
3478 1572 1122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 2133 2133
Size: 6172
SacI G_AGCT'C
Cuts at: 2936 3020 2936
Size: 84 6088
SacII CC_GC'GG
Cuts at: 2150 2150
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SanDI GG'GwC_CC
Cuts at: 2075 2075
Size: 6172
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SfcI C'TryA_G
Cuts at: 826 1045 2398 3520 4655 5336 5527 826
Size: 219 1353 1122 1135 681 191 1471
Fragments arranged by size:
1471 1353 1135 1122 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2143 2143
Size: 6172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 2966 3134 3302 1700
Size: 1266 168 168 4570
Fragments arranged by size:
4570 1266 168 168
SmaI CCC'GGG
Cuts at: 1872 3499 1872
Size: 1627 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4276 5177 5439 820
Size: 197 926 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 2417 2585 2669 2753 2921 3005 3089 3257
Size: 900 168 84 84 168 84 84 168
Cuts at: 3257 1517
Size: 4432
Fragments arranged by size:
4432 900 168 168 168 84 84 84
84
StyI C'CwwG_G
Cuts at: 513 1065 2254 3611 513
Size: 552 1189 1357 3074
Fragments arranged by size:
3074 1357 1189 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2708 2876 4199 4216 4369 4554 4999
Size: 1255 1128 168 1323 17 153 185 445
Cuts at: 4999 325
Size: 1498
Fragments arranged by size:
1498 1323 1255 1128 445 185 168 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4418 6130 96
Size: 274 80 33 51 1443 2441 1712 138
Fragments arranged by size:
2441 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 3497 1870
Size: 1627 4545
XmnI GAAnn'nnTTC
Cuts at: 2501 3062 3173 3341 3425 4301 2501
Size: 561 111 168 84 876 4372
Fragments arranged by size:
4372 876 561 168 111 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI
SexAI SfoI SrfI SwaI