Restriction map for
pFN21AB9665
(Circular) (Six-base) MAPSORT of: pFN21AB9665.seq Check: 7005 from: 1 to: 5974
pFN21AB9665 5974 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2259 2679 3016 4037 5421 5689 1632
Size: 375 252 420 337 1021 1384 268 1917
Fragments arranged by size:
1917 1384 1021 420 375 337 268 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 3038 4873 5850 1203
Size: 1835 1835 977 1327
Fragments arranged by size:
1835 1835 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 2654 2991 1166
Size: 1488 337 4149
Fragments arranged by size:
4149 1488 337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaLI G'TGCA_C
Cuts at: 2758 4031 5187 2758
Size: 1273 1156 3545
Fragments arranged by size:
3545 1273 1156
ApoI r'AATT_y
Cuts at: 1493 2115 2309 2393 2729 3283 3509 3661 5770
Size: 622 194 84 336 554 226 152 2109
Cuts at: 5770 1493
Size: 1697
Fragments arranged by size:
2109 1697 622 554 336 226 194 152
84
AseI AT'TA_AT
Cuts at: 160 1032 2297 4528 160
Size: 872 1265 2231 1606
Fragments arranged by size:
2231 1606 1265 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BamHI G'GATC_C
Cuts at: 2141 3304 3671 2141
Size: 1163 367 4444
Fragments arranged by size:
4444 1163 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2035 2203 2455 3293 1391
Size: 644 168 252 838 4072
Fragments arranged by size:
4072 838 644 252 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3171 928
Size: 971 1272 3731
Fragments arranged by size:
3731 1272 971
BbvCI CC'TCA_GC
Cuts at: 3075 3075
Size: 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2256 1548
Size: 708 5266
BfrBI ATG'CAT
Cuts at: 2503 2587 3017 2503
Size: 84 430 5460
Fragments arranged by size:
5460 430 84
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2037 2289 2373 2541 2787 5969 2037
Size: 252 84 168 246 3182 2042
Fragments arranged by size:
3182 2042 252 246 168 84
BlpI GC'TnA_GC
Cuts at: 2875 3391 2875
Size: 516 5458
Bme1580I G_kGCm'C
Cuts at: 1790 2762 4035 5191 1790
Size: 972 1273 1156 2573
Fragments arranged by size:
2573 1273 1156 972
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 2832 2916
Size: 416 53 83 399 24 658 757 84
Cuts at: 2916 3705 4663 442
Size: 789 958 1753
Fragments arranged by size:
1753 958 789 757 658 416 399 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2255 2339 2507 2591 2759 4634 1239
Size: 932 84 84 168 84 168 1875 2579
Fragments arranged by size:
2579 1875 932 168 168 84 84 84
Bpu10I CC'TnA_GC
Cuts at: 3075 3075
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4099 4964 5262 4099
Size: 865 298 4811
Fragments arranged by size:
4811 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3092 1186
Size: 1906 4068
BseYI C'CCAG_C
Cuts at: 5177 5673 5177
Size: 496 5478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2788 2872 3124 1163
Size: 573 1052 84 252 4013
Fragments arranged by size:
4013 1052 573 252 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2035 2203 2455 2762 2876 4035 4120 5191 2035
Size: 168 252 307 114 1159 85 1071 2818
Fragments arranged by size:
2818 1159 1071 307 252 168 114 85
BsmI GAATG_Cn'
Cuts at: 3265 3485 3578 3265
Size: 220 93 5661
Fragments arranged by size:
5661 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2203 2455 2762 2876 3293 4035
Size: 399 245 168 252 307 114 417 742
Cuts at: 4035 4120 5191 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 417 399 307 252 245
168 114 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3142 3863 5593 3142
Size: 721 1730 3523
Fragments arranged by size:
3523 1730 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2670 4469 4643 65
Size: 1183 1422 1799 174 1396
Fragments arranged by size:
1799 1422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 3206 96
Size: 3110 2864
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2766 2850 794
Size: 1972 84 3918
Fragments arranged by size:
3918 1972 84
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2037 2141 2289 2373 2541 2787 3304 3671
Size: 65 104 148 84 168 246 517 367
Cuts at: 3671 4056 4073 5525 5611 5623 5969 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 517 385 367 346 246 168
148 104 86 84 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3490 4284 4304 2851
Size: 639 794 20 4521
Fragments arranged by size:
4521 794 639 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2766 2766
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3904 1353
Size: 2551 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2033 2201 2453 2033
Size: 168 252 5554
Fragments arranged by size:
5554 252 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2171 2255 2259 2339 2507 2591
Size: 393 375 164 84 4 80 168 84
Cuts at: 2591 2679 2759 3016 4037 4634 5421 5689 1239
Size: 88 80 257 1021 597 787 268 1524
Fragments arranged by size:
1524 1021 787 597 393 375 268 257
168 164 88 84 84 80 80 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2277 2277
Size: 5974
EcoRI G'AATT_C
Cuts at: 2115 2309 2393 2729 3283 3661 2115
Size: 194 84 336 554 378 4428
Fragments arranged by size:
4428 554 378 336 194 84
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 4615 4647 4689
Size: 32 135 32 450 32 2561 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 2561 450 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2186 2523 2944 748
Size: 1438 337 421 3778
Fragments arranged by size:
3778 1438 421 337
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2480 5088 5272 1305
Size: 1175 2608 184 2007
Fragments arranged by size:
2608 2007 1175 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2082 2402 2586 2654 2839 2991
Size: 648 843 73 320 184 68 185 152
Cuts at: 2991 3932 4291 4450 518
Size: 941 359 159 2042
Fragments arranged by size:
2042 941 843 648 359 320 185 184
159 152 73 68
MspA1I CmG'CkG
Cuts at: 1606 3390 4067 5215 5460 1606
Size: 1784 677 1148 245 2120
Fragments arranged by size:
2120 1784 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5422
NdeI CA'TA_TG
Cuts at: 387 2583 387
Size: 2196 3778
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NsiI A_TGCA'T
Cuts at: 2505 2589 3019 2505
Size: 84 430 5460
Fragments arranged by size:
5460 430 84
NspI r_CATG'y
Cuts at: 1132 1229 2507 2591 3021 3042 3332 4877 1132
Size: 97 1278 84 430 21 290 1545 2229
Fragments arranged by size:
2229 1545 1278 430 290 97 84 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 3038 4873 3038
Size: 1835 4139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
SacI G_AGCT'C
Cuts at: 2035 2203 2455 2035
Size: 168 252 5554
Fragments arranged by size:
5554 252 168
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 3322 4457 5138 5329 826
Size: 219 2277 1135 681 191 1471
Fragments arranged by size:
2277 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4078 4979 5241 820
Size: 197 926 2135 901 262 1553
Fragments arranged by size:
2135 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 3413 513
Size: 552 2348 3074
Fragments arranged by size:
3074 2348 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4001 4018 4171 4356 4801 325
Size: 1255 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2515 2646 3041
Size: 274 80 33 51 1443 538 131 395
Cuts at: 3041 3206 4220 5932 96
Size: 165 1014 1712 138
Fragments arranged by size:
1712 1443 1014 538 395 274 165 138
131 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 2020 2692 2861 3029 4103 2020
Size: 672 169 168 1074 3891
Fragments arranged by size:
3891 1074 672 169 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI