Restriction map for
pFN21AB9664
(Circular) (Six-base) MAPSORT of: pFN21AB9664.seq Check: 3782 from: 1 to: 6079
pFN21AB9664 6079 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2287 3889 278
Size: 53 83 186 936 751 1602 2468
Fragments arranged by size:
2468 1602 936 751 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 2635 2971 3055 3307 4142 5526 5794
Size: 588 415 336 84 252 835 1384 268
Cuts at: 5794 1632
Size: 1917
Fragments arranged by size:
1917 1384 835 588 415 336 268 252
84
AfeI AGC'GCT
Cuts at: 2996 2996
Size: 6079
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 4978 5955 1203
Size: 3775 977 1327
Fragments arranged by size:
3775 1327 977
AgeI A'CCGG_T
Cuts at: 3099 4910 3099
Size: 1811 4268
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 3822 5394 3822
Size: 1572 4507
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 3388 3614 3766 5875 1493
Size: 1895 226 152 2109 1697
Fragments arranged by size:
2109 1895 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2746 3404 1870
Size: 73 803 658 4545
Fragments arranged by size:
4545 803 658 73
BamHI G'GATC_C
Cuts at: 2446 3409 3776 2446
Size: 963 367 4749
Fragments arranged by size:
4749 963 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 4855 618
Size: 325 217 627 1613 1455 1842
Fragments arranged by size:
1842 1613 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2747 2831 2915 3083 3167 3335 3398 1391
Size: 1356 84 84 168 84 168 63 4072
Fragments arranged by size:
4072 1356 168 168 84 84 84 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2424 4690 136
Size: 107 122 71 631 605 752 2266 1525
Fragments arranged by size:
2266 1525 752 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 4140 5296 1790
Size: 2350 1156 2573
Fragments arranged by size:
2573 2350 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2967 3219 3303 4739 1239
Size: 1728 252 84 1436 2579
Fragments arranged by size:
2579 1728 1436 252 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2194 2815 4204 5069 5367 2194
Size: 621 1389 865 298 2906
Fragments arranged by size:
2906 1389 865 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2679 4742 882
Size: 617 1180 2063 2219
Fragments arranged by size:
2219 2063 1180 617
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 2485 2586 6073 2485
Size: 101 3487 2491
Fragments arranged by size:
3487 2491 101
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2284 3886 4268 4903
Size: 53 83 186 936 751 1602 382 635
Cuts at: 4903 275
Size: 1451
Fragments arranged by size:
1602 1451 936 751 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3099 4512 4910 5184 5331 5742 3099
Size: 1413 398 274 147 411 3436
Fragments arranged by size:
3436 1413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2734 2764 2818 2848 1178
Size: 30 165 30 1331 30 54 30 4409
Fragments arranged by size:
4409 1331 165 54 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 2984 3248 1186
Size: 1357 441 264 4017
Fragments arranged by size:
4017 1357 441 264
BseYI C'CCAG_C
Cuts at: 5282 5778 5282
Size: 496 5583
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2906 2990 3322 1163
Size: 573 1170 84 332 3920
Fragments arranged by size:
3920 1170 573 332 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3529 4290 5318 1540
Size: 275 186 1528 761 1028 2301
Fragments arranged by size:
2301 1528 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2747 2831 2915 3083 3167 3335 4140 4225 5296
Size: 84 84 168 84 168 805 85 1071
Cuts at: 5296 2747
Size: 3530
Fragments arranged by size:
3530 1071 805 168 168 85 84 84
84
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
BsmBI CGTCTCn'nnnn_
Cuts at: 2291 2291
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2747 2831 2915 3083 3167 3335 3398
Size: 399 957 84 84 168 84 168 63
Cuts at: 3398 4140 4225 5296 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 957 742 399 168 168 85
84 84 84 63
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3012 3434 844
Size: 2168 422 3489
Fragments arranged by size:
3489 2168 422
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2726 4574 4748 65
Size: 1183 1478 1848 174 1396
Fragments arranged by size:
1848 1478 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3099 4723 4910 1700
Size: 110 1289 1624 187 2869
Fragments arranged by size:
2869 1624 1289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 2169 4883 2169
Size: 2714 3365
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2446 3169 3370 3409 3776 4161 4178 5630
Size: 474 723 201 39 367 385 17 1452
Cuts at: 5630 5716 5728 6074 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 723 474 385 367 346 201
86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2262 2262
Size: 6079
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 2607 3595 4389 4409 2607
Size: 988 794 20 4277
Fragments arranged by size:
4277 988 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2425 3526
Size: 54 1425 50 275 18 177 418 1101
Cuts at: 3526 4415 6044 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1101 889 418 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2239 4009 1353
Size: 804 82 1770 3423
Fragments arranged by size:
3423 1770 804 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2745 2829 2913 3081 3165 3333 2745
Size: 84 84 168 84 168 5491
Fragments arranged by size:
5491 168 168 84 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2635 2967 2971 3055 3219 3303
Size: 393 588 415 332 4 84 164 84
Cuts at: 3303 3307 4142 4739 5526 5794 1239
Size: 4 835 597 787 268 1524
Fragments arranged by size:
1524 835 787 597 588 415 393 332
268 164 84 84 4 4
EcoO109I rG'GnC_Cy
Cuts at: 3039 3039
Size: 6079
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 2998 5226 1678
Size: 1320 2228 2531
Fragments arranged by size:
2531 2228 1320
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2734 2766 2818 2850 2974
Size: 32 135 32 1162 32 52 32 124
Cuts at: 2974 3006 3154 3186 4720 4752 4794 4826 1373
Size: 32 148 32 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 1162 148 135 124 52 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2096 3423 3669 669
Size: 988 281 158 1327 246 3079
Fragments arranged by size:
3079 1327 988 281 246 158
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3360 5193 5377 1305
Size: 2055 1833 184 2007
Fragments arranged by size:
2055 2007 1833 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2427 10
Size: 54 1425 343 177 418 3662
Fragments arranged by size:
3662 1425 418 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2592 2778 4037 4396 4555 518
Size: 648 1426 186 1259 359 159 2042
Fragments arranged by size:
2042 1426 1259 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 3355 3495 4172 5320 5565 1606
Size: 1749 140 677 1148 245 2120
Fragments arranged by size:
2120 1749 1148 677 245 140
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 2593 387
Size: 2206 3873
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3437 4982 1132
Size: 97 2208 1545 2229
Fragments arranged by size:
2229 2208 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 2793 2877 3649 2793
Size: 84 772 5223
Fragments arranged by size:
5223 772 84
PspXI vC'TCGA_Gb
Cuts at: 2746 2746
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2614 3431 830
Size: 1784 817 3478
Fragments arranged by size:
3478 1784 817
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
SacI G_AGCT'C
Cuts at: 2747 2831 2915 3083 3167 3335 2747
Size: 84 84 168 84 168 5491
Fragments arranged by size:
5491 168 168 84 84 84
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 2610 3427 4562 5243 5434 826
Size: 219 1565 817 1135 681 191 1471
Fragments arranged by size:
1565 1471 1135 817 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 3099 1700
Size: 1399 4680
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2209 2746 2830 4183 5084 5346
Size: 197 926 266 537 84 1353 901 262
Cuts at: 5346 820
Size: 1553
Fragments arranged by size:
1553 1353 926 901 537 266 262 197
84
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 3518 513
Size: 552 939 1514 3074
Fragments arranged by size:
3074 1514 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2012 3022 3274
Size: 274 80 33 51 1443 35 1010 252
Cuts at: 3274 4325 6037 96
Size: 1051 1712 138
Fragments arranged by size:
1712 1443 1051 1010 274 252 138 80
51 35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6039 6071 6039
Size: 32 6047
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2552 2552
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 2746 1943
Size: 803 5276
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 2732 2816 3068 3152 3320 4208 2732
Size: 84 252 84 168 888 4603
Fragments arranged by size:
4603 888 252 168 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2285 3887 276
Size: 53 83 186 936 751 1602 2468
Fragments arranged by size:
2468 1602 936 751 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI