Restriction map for
pFN21AB9662
(Circular) (Six-base) MAPSORT of: pFN21AB9662.seq Check: 1933 from: 1 to: 5716
pFN21AB9662 5716 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 2469 3037 2469
Size: 568 5148
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 2930 3779 5163 5431 1632
Size: 863 435 849 1384 268 1917
Fragments arranged by size:
1917 1384 863 849 435 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 2487 4615 5592 1203
Size: 1284 2128 977 1327
Fragments arranged by size:
2128 1327 1284 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaI G_GGCC'C
Cuts at: 2531 2531
Size: 5716
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2639 3025 3251 3403 5512 1493
Size: 1146 386 226 152 2109 1697
Fragments arranged by size:
2109 1697 1146 386 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2202 2509 2669 3041 1870
Size: 73 183 76 307 160 372 4545
Fragments arranged by size:
4545 372 307 183 160 76 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 2260 2469 3037 4492
Size: 325 217 627 404 69 209 568 1455
Cuts at: 4492 618
Size: 1842
Fragments arranged by size:
1842 1455 627 568 404 325 217 209
69
BanII G_rGCy'C
Cuts at: 1391 2531 3035 1391
Size: 1140 504 4072
Fragments arranged by size:
4072 1140 504
BbeI G_GCGC'C
Cuts at: 2264 2264
Size: 5716
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2991 928
Size: 971 1092 3653
Fragments arranged by size:
3653 1092 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2314 4327 136
Size: 107 122 71 631 605 642 2013 1525
Fragments arranged by size:
2013 1525 642 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 2175 3133 2175
Size: 958 4758
Bme1580I G_kGCm'C
Cuts at: 1790 2531 3777 4933 1790
Size: 741 1246 1156 2573
Fragments arranged by size:
2573 1246 1156 741
BmgBI CAC'GTC
Cuts at: 1318 2278 2490 1318
Size: 960 212 4544
Fragments arranged by size:
4544 960 212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2795 3447 4405
Size: 416 53 83 399 24 1378 652 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1378 958 652 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2007 2867 4376 1239
Size: 768 860 1509 2579
Fragments arranged by size:
2579 1509 860 768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 3841 4706 5004 2372
Size: 1469 865 298 3084
Fragments arranged by size:
3084 1469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2537 3507 493
Size: 711 1333 970 2702
Fragments arranged by size:
2702 1333 970 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2261 3523 3905 4540
Size: 53 83 186 936 728 1262 382 635
Cuts at: 4540 275
Size: 1451
Fragments arranged by size:
1451 1262 936 728 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2494 2497 1186
Size: 1081 227 3 4405
Fragments arranged by size:
4405 1081 227 3
BseYI C'CCAG_C
Cuts at: 2568 4919 5415 2568
Size: 2351 496 2869
Fragments arranged by size:
2869 2351 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2413 2836 1163
Size: 573 677 423 4043
Fragments arranged by size:
4043 677 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2653 3166 3927 4955 1540
Size: 275 186 36 616 513 761 1028 2301
Fragments arranged by size:
2301 1028 761 616 513 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2133 2370 2433 2853 3777 3862 4933 2133
Size: 237 63 420 924 85 1071 2916
Fragments arranged by size:
2916 1071 924 420 237 85 63
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2370 2433 2531 2853 3035 3777
Size: 399 343 237 63 98 322 182 742
Cuts at: 3777 3862 4933 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 322 237 182
98 85 63
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3071 844
Size: 2227 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2298 3603 1394
Size: 429 475 1305 3507
Fragments arranged by size:
3507 1305 475 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2894 4360 4547 1700
Size: 110 1084 1466 187 2869
Fragments arranged by size:
2869 1466 1084 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 96
Size: 2187 3529
BssHII G'CGCG_C
Cuts at: 1925 2554 2841 1925
Size: 629 287 4800
Fragments arranged by size:
4800 629 287
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711
Size: 1074 367 385 17 1452 86 12 346
Cuts at: 5711 1972
Size: 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2465 2465
Size: 5716
BtgI C'CryG_G
Cuts at: 513 1065 2004 2183 2493 3009 513
Size: 552 939 179 310 516 3220
Fragments arranged by size:
3220 939 552 516 310 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 3232 4026 4046 2414
Size: 818 794 20 4084
Fragments arranged by size:
4084 818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DraIII CAC_nnn'GTG
Cuts at: 2814 2814
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 3163 4052
Size: 54 1425 50 275 18 204 1129 889
Cuts at: 4052 5681 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1129 889 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 3163 1537
Size: 275 222 1129 4090
Fragments arranged by size:
4090 1129 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2691 2745 3646 1353
Size: 719 619 54 901 3423
Fragments arranged by size:
3423 901 719 619 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2495 2867 2930 3779 4376 5163
Size: 393 375 488 372 63 849 597 787
Cuts at: 5163 5431 1239
Size: 268 1524
Fragments arranged by size:
1524 849 787 597 488 393 375 372
268 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2022 2022
Size: 5716
EcoO109I rG'GnC_Cy
Cuts at: 2199 2206 2527 2528 2199
Size: 7 321 1 5387
Fragments arranged by size:
5387 321 7 1
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2015 2113 2264 4863 1678
Size: 337 98 151 2599 2531
Fragments arranged by size:
2599 2531 337 151 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4357 4389 4431 4463 1373
Size: 32 135 32 2785 32 42 32 2626
Fragments arranged by size:
2785 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2942 748
Size: 2194 3522
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KasI G'GCGC_C
Cuts at: 2260 2260
Size: 5716
KpnI G_GTAC'C
Cuts at: 2473 3041 2473
Size: 568 5148
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4830 5014 1305
Size: 3525 184 2007
Fragments arranged by size:
3525 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2767 3674 4033 4192 518
Size: 648 1326 275 907 359 159 2042
Fragments arranged by size:
2042 1326 907 648 359 275 159
MspA1I CmG'CkG
Cuts at: 1606 2151 2185 2217 2339 2401 2634 3132 3809
Size: 545 34 32 122 62 233 498 677
Cuts at: 3809 4957 5202 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 545 498 245 233 122
62 34 32
NarI GG'CG_CC
Cuts at: 2261 2261
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 2004 2493 3009 513
Size: 552 939 489 516 3220
Fragments arranged by size:
3220 939 552 516 489
NdeI CA'TA_TG
Cuts at: 387 2777 387
Size: 2390 3326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5716
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 1108
Size: 1629 4087
PfoI T'CCnGG_A
Cuts at: 2023 2406 2023
Size: 383 5333
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PspOMI G'GGCC_C
Cuts at: 2527 2527
Size: 5716
PstI C_TGCA'G
Cuts at: 830 2098 2956 3068 830
Size: 1268 858 112 3478
Fragments arranged by size:
3478 1268 858 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2217 2634 2217
Size: 417 5299
SacII CC_GC'GG
Cuts at: 2186 2186
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 5716
SfcI C'TryA_G
Cuts at: 826 1045 2094 2952 3064 4199 4880 5071 826
Size: 219 1049 858 112 1135 681 191 1471
Fragments arranged by size:
1471 1135 1049 858 681 219 191 112
SfoI GGC'GCC
Cuts at: 2262 2262
Size: 5716
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 2204 3043 1872
Size: 332 839 4545
Fragments arranged by size:
4545 839 332
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 3820 4721 4983 820
Size: 197 926 444 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 444 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 2100 2789 1517
Size: 583 689 4444
Fragments arranged by size:
4444 689 583
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 2493 3009 3155 513
Size: 552 939 477 12 516 146 3074
Fragments arranged by size:
3074 939 552 516 477 146 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3743 3760 3913 4098 4543 325
Size: 1255 2163 17 153 185 445 1498
Fragments arranged by size:
2163 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 3962 5674
Size: 274 80 33 51 1443 306 1679 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1712 1679 1443 306 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2639 2671 5676 5708 2639
Size: 32 3005 32 2647
Fragments arranged by size:
3005 2647 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2923 2923
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 2202 3041 1870
Size: 332 839 4545
Fragments arranged by size:
4545 839 332
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI
SfiI SrfI SwaI