Restriction map for
pFN21AB9657
(Circular) (Six-base) MAPSORT of: pFN21AB9657.seq Check: 6810 from: 1 to: 5311
pFN21AB9657 5311 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 2016 4210 5187 1203
Size: 813 2194 977 1327
Fragments arranged by size:
2194 1327 977 813
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 2374 3054 4626 2374
Size: 680 1572 3059
Fragments arranged by size:
3059 1572 680
ApaI G_GGCC'C
Cuts at: 2160 2160
Size: 5311
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2636 1870
Size: 73 693 4545
Fragments arranged by size:
4545 693 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 4087 618
Size: 325 217 627 845 1455 1842
Fragments arranged by size:
1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2160 2630 1391
Size: 769 470 4072
Fragments arranged by size:
4072 769 470
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3502 3536 1903
Size: 34 1565 34 3678
Fragments arranged by size:
3678 1565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2080 3922 136
Size: 107 122 71 631 605 408 1842 1525
Fragments arranged by size:
1842 1525 631 605 408 122 107 71
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2432 2552 2728 2432
Size: 120 176 5015
Fragments arranged by size:
5015 176 120
Bme1580I G_kGCm'C
Cuts at: 1790 2160 3372 4528 1790
Size: 370 1212 1156 2573
Fragments arranged by size:
2573 1212 1156 370
BmgBI CAC'GTC
Cuts at: 1318 2017 1318
Size: 699 4612
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2362 3042 4000
Size: 416 53 83 399 24 945 680 958
Cuts at: 4000 442
Size: 1753
Fragments arranged by size:
1753 958 945 680 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2593 3971 1239
Size: 1238 116 1378 2579
Fragments arranged by size:
2579 1378 1238 116
Bpu10I CC'TnA_GC
Cuts at: 2054 2054
Size: 5311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 3436 4301 4599 2435
Size: 1001 865 298 3147
Fragments arranged by size:
3147 1001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3974 882
Size: 617 2475 2219
Fragments arranged by size:
2475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 3118 3500 4135
Size: 53 83 186 936 648 937 382 635
Cuts at: 4135 275
Size: 1451
Fragments arranged by size:
1451 937 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 1186
Size: 1144 4167
BseYI C'CCAG_C
Cuts at: 4514 5010 4514
Size: 496 4815
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4738
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 2761 3522 4550 1540
Size: 275 186 42 718 761 1028 2301
Fragments arranged by size:
2301 1028 761 718 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 3372 3457 4528 3372
Size: 85 1071 4155
Fragments arranged by size:
4155 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2630 3372 3457 4528 1391
Size: 399 370 470 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 470 399 370 85
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2666 844
Size: 1822 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2097 2548 3198 1394
Size: 429 274 451 650 3507
Fragments arranged by size:
3507 650 451 429 274
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2079 3955 4142 1700
Size: 110 228 41 1876 187 2869
Fragments arranged by size:
2869 1876 228 187 110 41
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 2543 3371 4383 2543
Size: 828 1012 3471
Fragments arranged by size:
3471 1012 828
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2641 3008 3393 3410 4862 4948 4960 5306
Size: 669 367 385 17 1452 86 12 346
Cuts at: 5306 1972
Size: 1977
Fragments arranged by size:
1977 1452 669 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2169 513
Size: 552 1104 3655
Fragments arranged by size:
3655 1104 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2340 2460
Size: 54 1425 50 275 18 210 300 120
Cuts at: 2460 2758 3647 5276 8
Size: 298 889 1629 43
Fragments arranged by size:
1629 1425 889 300 298 275 210 120
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 2758 1537
Size: 275 228 718 4090
Fragments arranged by size:
4090 718 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 3241 1353
Size: 711 1177 3423
Fragments arranged by size:
3423 1177 711
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2178 3898 4272 4418 387
Size: 1064 138 589 1720 374 146 1280
Fragments arranged by size:
1720 1280 1064 589 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 2593 3374 3971 4758 5026 1239
Size: 393 845 116 781 597 787 268 1524
Fragments arranged by size:
1524 845 787 781 597 393 268 116
EcoNI CCTnn'n_nnAGG
Cuts at: 2483 2483
Size: 5311
EcoO109I rG'GnC_Cy
Cuts at: 2156 2488 2586 2156
Size: 332 98 4881
Fragments arranged by size:
4881 332 98
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 2359 4458 1678
Size: 681 2099 2531
Fragments arranged by size:
2531 2099 681
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2901 669
Size: 988 281 717 246 3079
Fragments arranged by size:
3079 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2164 4425 4609 1305
Size: 859 2261 184 2007
Fragments arranged by size:
2261 2007 859 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 2979
Fragments arranged by size:
2979 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3269 3628 3787 518
Size: 648 2103 359 159 2042
Fragments arranged by size:
2103 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2289 2332 2727 3404 4552 4797 1606
Size: 565 118 43 395 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 395 245 118 43
NaeI GCC'GGC
Cuts at: 2040 2040
Size: 5311
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4759
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NgoMIV G'CCGG_C
Cuts at: 2038 2038
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 2215 1348
Size: 867 4444
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5311
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 5311
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PspOMI G'GGCC_C
Cuts at: 2156 2156
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2325 2531 2663 830
Size: 1495 206 132 3478
Fragments arranged by size:
3478 1495 206 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
SacII CC_GC'GG
Cuts at: 2172 2172
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SapI GCTCTTCn'nnn_
Cuts at: 2064 2064
Size: 5311
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SexAI A'CCwGG_T
Cuts at: 2484 2484
Size: 5311
SfcI C'TryA_G
Cuts at: 826 1045 2321 2527 2659 3794 4475 4666 826
Size: 219 1276 206 132 1135 681 191 1471
Fragments arranged by size:
1471 1276 1135 681 219 206 191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2638 1872
Size: 766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2450 3415 4316 4578 820
Size: 197 926 507 965 901 262 1553
Fragments arranged by size:
1553 965 926 901 507 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2750 513
Size: 552 1685 3074
Fragments arranged by size:
3074 1685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2381 2557 3338 3355 3508 3693 4138
Size: 1255 801 176 781 17 153 185 445
Cuts at: 4138 325
Size: 1498
Fragments arranged by size:
1498 1255 801 781 445 185 176 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2636 1870
Size: 766 4545
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SanDI SfiI SfoI SrfI SwaI XcmI