Restriction map for
pFN21AB9655
(Circular) (Six-base) MAPSORT of: pFN21AB9655.seq Check: 5255 from: 1 to: 6751
pFN21AB9655 6751 bp
With 182 enzymes: SgfI *
July 29, 2009 14:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2238 4561 278
Size: 53 83 186 936 702 2323 2468
Fragments arranged by size:
2468 2323 936 702 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4094 1937
Size: 2157 4594
Acc65I G'GTAC_C
Cuts at: 4072 4072
Size: 6751
AclI AA'CG_TT
Cuts at: 1563 4878 5251 1563
Size: 3315 373 3063
Fragments arranged by size:
3315 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4814 6198 6466 1632
Size: 3182 1384 268 1917
Fragments arranged by size:
3182 1917 1384 268
AfeI AGC'GCT
Cuts at: 3782 3782
Size: 6751
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6554
AflIII A'CryG_T
Cuts at: 1203 5650 6627 1203
Size: 4447 977 1327
Fragments arranged by size:
4447 1327 977
AgeI A'CCGG_T
Cuts at: 5582 5582
Size: 6751
AhdI GACnn_n'nnGTC
Cuts at: 5480 5480
Size: 6751
AleI CACnn'nnGTG
Cuts at: 1166 2843 3941 1166
Size: 1677 1098 3976
Fragments arranged by size:
3976 1677 1098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6751
AlwNI CAG_nnn'CTG
Cuts at: 4494 6066 4494
Size: 1572 5179
ApaI G_GGCC'C
Cuts at: 3274 3362 3274
Size: 88 6663
ApaLI G'TGCA_C
Cuts at: 4808 5964 4808
Size: 1156 5595
ApoI r'AATT_y
Cuts at: 1493 2051 2651 4060 4286 4438 6547 1493
Size: 558 600 1409 226 152 2109 1697
Fragments arranged by size:
2109 1697 1409 600 558 226 152
AscI GG'CGCG_CC
Cuts at: 3435 3435
Size: 6751
AseI AT'TA_AT
Cuts at: 160 1032 5305 160
Size: 872 4273 1606
Fragments arranged by size:
4273 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
AvaI C'yCGr_G
Cuts at: 1870 1943 3266 3343 3362 3430 3889 4076 1870
Size: 73 1323 77 19 68 459 187 4545
Fragments arranged by size:
4545 1323 459 187 77 73 68 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2606 2639 2606
Size: 33 6718
BamHI G'GATC_C
Cuts at: 2090 2782 4081 4448 2090
Size: 692 1299 367 4393
Fragments arranged by size:
4393 1299 692 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2602 2661 3641 3668 3734
Size: 325 217 627 815 59 980 27 66
Cuts at: 3734 3800 4072 5527 618
Size: 66 272 1455 1842
Fragments arranged by size:
1842 1455 980 815 627 325 272 217
66 66 59 27
BanII G_rGCy'C
Cuts at: 1391 2566 2896 3274 3362 3683 4070 1391
Size: 1175 330 378 88 321 387 4072
Fragments arranged by size:
4072 1175 387 378 330 321 88
BbeI G_GCGC'C
Cuts at: 3645 3738 3804 3645
Size: 93 66 6592
Fragments arranged by size:
6592 93 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2285 2319 2837 2871 4942
Size: 34 50 34 264 34 518 34 2071
Cuts at: 4942 4976 1903
Size: 34 3678
Fragments arranged by size:
3678 2071 518 264 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2501 4643 5853 2501
Size: 2142 1210 3399
Fragments arranged by size:
3399 2142 1210
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 5623
BfrBI ATG'CAT
Cuts at: 2869 2869
Size: 6751
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2644 3063 3612
Size: 107 122 71 631 605 972 419 549
Cuts at: 3612 3697 5362 136
Size: 85 1665 1525
Fragments arranged by size:
1665 1525 972 631 605 549 419 122
107 85 71
BglII A'GATC_T
Cuts at: 6746 6746
Size: 6751
BlpI GC'TnA_GC
Cuts at: 4168 4168
Size: 6751
Bme1580I G_kGCm'C
Cuts at: 1790 2607 3267 3274 3362 4812 5968 1790
Size: 817 660 7 88 1450 1156 2573
Fragments arranged by size:
2573 1450 1156 817 660 88 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6751
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2358 2898 4482
Size: 416 53 83 399 24 941 540 1584
Cuts at: 4482 5440 442
Size: 958 1753
Fragments arranged by size:
1753 1584 958 941 540 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6751
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3874 3874
Size: 6751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2162 2815 3865 5411 1239
Size: 794 129 653 1050 1546 2579
Fragments arranged by size:
2579 1546 1050 794 653 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3950 4876 5741 6039 3950
Size: 926 865 298 4662
Fragments arranged by size:
4662 926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2278 2738 5414 882
Size: 617 779 460 2676 2219
Fragments arranged by size:
2676 2219 779 617 460
BsaAI yAC'GTr
Cuts at: 493 1204 4542 493
Size: 711 3338 2702
Fragments arranged by size:
3338 2702 711
BsaBI GATnn'nnATC
Cuts at: 2678 2787 6745 2678
Size: 109 3958 2684
Fragments arranged by size:
3958 2684 109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 3464 3642 3735
Size: 53 83 186 936 702 1229 178 93
Cuts at: 3735 3801 3813 3854 4558 4940 5575 275
Size: 66 12 41 704 382 635 1451
Fragments arranged by size:
1451 1229 936 704 702 635 382 186
178 93 83 66 53 41 12
BsaWI w'CCGG_w
Cuts at: 2872 5184 5582 5856 6003 6414 2872
Size: 2312 398 274 147 411 3209
Fragments arranged by size:
3209 2312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 2594 1178
Size: 30 165 30 1131 30 30 5335
Fragments arranged by size:
5335 1131 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2485 2572 3770 3850 1186
Size: 1126 173 87 1198 80 4087
Fragments arranged by size:
4087 1198 1126 173 87 80
BseYI C'CCAG_C
Cuts at: 2589 3636 3984 5954 6450 2589
Size: 1047 348 1970 496 2890
Fragments arranged by size:
2890 1970 1047 496 348
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3092 3580 1163
Size: 573 1356 488 4334
Fragments arranged by size:
4334 1356 573 488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2520 3067 3717 3933 4201 4962
Size: 275 186 519 547 650 216 268 761
Cuts at: 4962 5990 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 650 547 519 275 268
216 186
BsiHKAI G_wGCw'C
Cuts at: 2019 2566 4812 4897 5968 2019
Size: 547 2246 85 1071 2802
Fragments arranged by size:
2802 2246 1071 547 85
BsmI GAATG_Cn'
Cuts at: 4262 4355 4262
Size: 93 6658
BsmBI CGTCTCn'nnnn_
Cuts at: 3845 3845
Size: 6751
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2566 2607 2896 3267 3274 3362
Size: 399 229 547 41 289 371 7 88
Cuts at: 3362 3683 4070 4812 4897 5968 1391
Size: 321 387 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 387 371 321
289 229 88 85 41 7
BspEI T'CCGG_A
Cuts at: 2872 6414 2872
Size: 3542 3209
BspHI T'CATG_A
Cuts at: 4640 6370 4640
Size: 1730 5021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4106 844
Size: 3262 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3610 3964 4638 1394
Size: 429 1787 354 674 3507
Fragments arranged by size:
3507 1787 674 429 354
BsrDI GCAATG_nn'
Cuts at: 65 1248 5246 5420 65
Size: 1183 3998 174 1396
Fragments arranged by size:
3998 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2398 2519 2658 3203 3287 3466 3477
Size: 110 588 121 139 545 84 179 11
Cuts at: 3477 3731 3928 5395 5582 1700
Size: 254 197 1467 187 2869
Fragments arranged by size:
2869 1467 588 545 254 197 187 179
139 121 110 84 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6751
BssHII G'CGCG_C
Cuts at: 1925 3435 3722 1925
Size: 1510 287 4954
Fragments arranged by size:
4954 1510 287
BssSI C'ACGA_G
Cuts at: 4811 5823 4811
Size: 1012 5739
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3262 794
Size: 2468 4283
BstBI TT'CG_AA
Cuts at: 5555 5555
Size: 6751
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6751
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6751
BstYI r'GATC_y
Cuts at: 1972 2090 2782 3921 4081 4448 4833 4850 6302
Size: 118 692 1139 160 367 385 17 1452
Cuts at: 6302 6388 6400 6746 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1139 692 385 367 346 160
118 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2514 2742 3282 3513 3711 513
Size: 552 1449 228 540 231 198 3553
Fragments arranged by size:
3553 1449 552 540 231 228 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3088 487
Size: 683 56 1862 4150
Fragments arranged by size:
4150 1862 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 2355 4267 5061 5081 2017
Size: 338 1912 794 20 3687
Fragments arranged by size:
3687 1912 794 338 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6751
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6716
DraI TTT'AAA
Cuts at: 4055 4055
Size: 6751
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2188 5758 809
Size: 507 872 3570 1802
Fragments arranged by size:
3570 1802 872 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2386 2517
Size: 54 1425 50 275 18 522 34 131
Cuts at: 2517 2545 3064 3285 3468 3510 3534 3601 3714
Size: 28 519 221 183 42 24 67 113
Cuts at: 3714 3729 3771 3930 4198 5087 6716 8
Size: 15 42 159 268 889 1629 43
Fragments arranged by size:
1629 1425 889 522 519 275 268 221
183 159 131 113 67 54 50 43
42 42 34 28 24 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2517 3064 3714 3930 4198 1537
Size: 275 705 547 650 216 268 4090
Fragments arranged by size:
4090 705 650 547 275 268 216
EarI CTCTTCn'nnn_
Cuts at: 1353 4681 1353
Size: 3328 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2103 2795 3934 5338 5712 5858
Size: 1064 138 514 692 1139 1404 374 146
Cuts at: 5858 387
Size: 1280
Fragments arranged by size:
1404 1280 1139 1064 692 514 374 146
138
EcoICRI GAG'CTC
Cuts at: 2564 2564
Size: 6751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2162 2815 3865 4814 5411 6198
Size: 393 401 129 653 1050 949 597 787
Cuts at: 6198 6466 1239
Size: 268 1524
Fragments arranged by size:
1524 1050 949 787 653 597 401 393
268 129
EcoNI CCTnn'n_nnAGG
Cuts at: 3752 3752
Size: 6751
EcoO109I rG'GnC_Cy
Cuts at: 2637 3270 3359 3427 3766 3826 2637
Size: 633 89 68 339 60 5562
Fragments arranged by size:
5562 633 339 89 68 60
EcoRI G'AATT_C
Cuts at: 4060 4438 4060
Size: 378 6373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2172 3352 2172
Size: 1180 5571
FspI TGC'GCA
Cuts at: 5257 5257
Size: 6751
HaeII r_GCGC'y
Cuts at: 1678 3575 3645 3711 3738 3784 3804 5898 1678
Size: 1897 70 66 27 46 20 2094 2531
Fragments arranged by size:
2531 2094 1897 70 66 46 27 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2170 2202 2258 2290 3941
Size: 32 135 32 598 32 56 32 1651
Cuts at: 3941 3973 5392 5424 5466 5498 1373
Size: 32 1419 32 42 32 2626
Fragments arranged by size:
2626 1651 1419 598 135 56 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4095 4341 669
Size: 988 281 2157 246 3079
Fragments arranged by size:
3079 2157 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6751
HpaI GTT'AAC
Cuts at: 4341 4341
Size: 6751
KasI G'GCGC_C
Cuts at: 3641 3734 3800 3641
Size: 93 66 6592
Fragments arranged by size:
6592 93 66
KpnI G_GTAC'C
Cuts at: 4076 4076
Size: 6751
MfeI C'AATT_G
Cuts at: 4350 4350
Size: 6751
MluI A'CGCG_T
Cuts at: 6627 6627
Size: 6751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 5865 6049 1305
Size: 1209 3351 184 2007
Fragments arranged by size:
3351 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4929
Fragments arranged by size:
4929 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 3941 4709 5068 5227 518
Size: 648 1677 1098 768 359 159 2042
Fragments arranged by size:
2042 1677 1098 768 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 3580 3713 3790 3843 3988 4167 4844
Size: 405 1569 133 77 53 145 179 677
Cuts at: 4844 5992 6237 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1569 1148 677 405 245 179 145
133 77 53
NaeI GCC'GGC
Cuts at: 2521 3289 3468 3733 3930 2521
Size: 768 179 265 197 5342
Fragments arranged by size:
5342 768 265 197 179
NarI GG'CG_CC
Cuts at: 3642 3735 3801 3642
Size: 93 66 6592
Fragments arranged by size:
6592 93 66
NcoI C'CATG_G
Cuts at: 513 1065 3282 513
Size: 552 2217 3982
Fragments arranged by size:
3982 2217 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6751
NgoMIV G'CCGG_C
Cuts at: 2519 3287 3466 3731 3928 2519
Size: 768 179 265 197 5342
Fragments arranged by size:
5342 768 265 197 179
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6751
NotI GC'GGCC_GC
Cuts at: 3714 4198 3714
Size: 484 6267
NsiI A_TGCA'T
Cuts at: 2871 2871
Size: 6751
NspI r_CATG'y
Cuts at: 1132 1229 2704 3020 4109 5654 1132
Size: 97 1475 316 1089 1545 2229
Fragments arranged by size:
2229 1545 1475 1089 316 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6751
PciI A'CATG_T
Cuts at: 5650 5650
Size: 6751
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2793 1108
Size: 1685 5066
PmeI GTTT'AAAC
Cuts at: 4055 4055
Size: 6751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 4820 4852 6333 6365 3083
Size: 32 1705 32 1481 32 3469
Fragments arranged by size:
3469 1705 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3427 3826 3427
Size: 399 6352
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6751
PsiI TTA'TAA
Cuts at: 4321 4321
Size: 6751
PspOMI G'GGCC_C
Cuts at: 3270 3358 3270
Size: 88 6663
PstI C_TGCA'G
Cuts at: 830 2311 3303 3585 3592 4103 830
Size: 1481 992 282 7 511 3478
Fragments arranged by size:
3478 1481 992 511 282 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6751
PvuII CAG'CTG
Cuts at: 2011 3843 2011
Size: 1832 4919
RsrII CG'GwC_CG
Cuts at: 2396 3376 2396
Size: 980 5771
SacI G_AGCT'C
Cuts at: 2566 2566
Size: 6751
SacII CC_GC'GG
Cuts at: 3714 3714
Size: 6751
SalI G'TCGA_C
Cuts at: 1936 4093 1936
Size: 2157 4594
SbfI CC_TGCA'GG
Cuts at: 3303 4103 3303
Size: 800 5951
ScaI AGT'ACT
Cuts at: 3662 4999 6711 3662
Size: 1337 1712 3702
Fragments arranged by size:
3702 1712 1337
SfcI C'TryA_G
Cuts at: 826 1045 2307 3299 3581 3588 4099 5234 5915
Size: 219 1262 992 282 7 511 1135 681
Cuts at: 5915 6106 826
Size: 191 1471
Fragments arranged by size:
1471 1262 1135 992 681 511 282 219
191 7
SfoI GGC'GCC
Cuts at: 3643 3736 3802 3643
Size: 93 66 6592
Fragments arranged by size:
6592 93 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6751
SmaI CCC'GGG
Cuts at: 1872 3268 3345 3364 3432 4078 1872
Size: 1396 77 19 68 646 4545
Fragments arranged by size:
4545 1396 646 77 68 19
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3965 4855 5756 6018 820
Size: 197 926 2022 890 901 262 1553
Fragments arranged by size:
2022 1553 926 901 890 262 197
SnaBI TAC'GTA
Cuts at: 493 4542 493
Size: 4049 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6751
SphI G_CATG'C
Cuts at: 1132 4109 1132
Size: 2977 3774
SspI AAT'ATT
Cuts at: 5 52 2758 4675 5
Size: 47 2706 1917 2081
Fragments arranged by size:
2706 2081 1917 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6751
StyI C'CwwG_G
Cuts at: 513 1065 2217 2557 3282 3327 3822 4011 4190
Size: 552 1152 340 725 45 495 189 179
Cuts at: 4190 513
Size: 3074
Fragments arranged by size:
3074 1152 725 552 495 340 189 179
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2603 4778 4795 4948 5133 5578 325
Size: 1255 1023 2175 17 153 185 445 1498
Fragments arranged by size:
2175 1498 1255 1023 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3660 4997 6709
Size: 274 80 33 51 1443 1683 1337 1712
Cuts at: 6709 96
Size: 138
Fragments arranged by size:
1712 1683 1443 1337 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6711 6743 6711
Size: 32 6719
Tth111I GACn'n_nGTC
Cuts at: 1316 2270 1316
Size: 954 5797
XbaI T'CTAG_A
Cuts at: 4087 4087
Size: 6751
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2137 3478 3745 2137
Size: 1341 267 5143
Fragments arranged by size:
5143 1341 267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6751
XmaI C'CCGG_G
Cuts at: 1870 3266 3343 3362 3430 4076 1870
Size: 1396 77 19 68 646 4545
Fragments arranged by size:
4545 1396 646 77 68 19
XmnI GAAnn'nnTTC
Cuts at: 4880 4880
Size: 6751
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2236 4559 276
Size: 53 83 186 936 702 2323 2468
Fragments arranged by size:
2468 2323 936 702 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BbvCI Bpu10I BsiWI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI NruI PacI PfoI PmlI
PspXI PsrI SanDI SapI SexAI SfiI SrfI SwaI