Restriction map for
pFN21AB9653
(Circular) (Six-base) MAPSORT of: pFN21AB9653.seq Check: 2421 from: 1 to: 5533
pFN21AB9653 5533 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2194 2876 1937
Size: 257 682 4594
Fragments arranged by size:
4594 682 257
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3596 4980 5248 1632
Size: 1964 1384 268 1917
Fragments arranged by size:
1964 1917 1384 268
AfeI AGC'GCT
Cuts at: 2126 2126
Size: 5533
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 2443 2577 4432 5409 1203
Size: 1240 134 1855 977 1327
Fragments arranged by size:
1855 1327 1240 977 134
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 3276 4848 3276
Size: 1572 3961
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2842 3068 3220 5329 1493
Size: 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 2689 2698 2741 2858 1870
Size: 73 462 284 9 43 117 4545
Fragments arranged by size:
4545 462 284 117 73 43 9
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2290 2345 2380 2612
Size: 325 217 627 345 158 55 35 232
Cuts at: 2612 2854 4309 618
Size: 242 1455 1842
Fragments arranged by size:
1842 1455 627 345 325 242 232 217
158 55 35
BanII G_rGCy'C
Cuts at: 1391 2068 2534 2852 1391
Size: 677 466 318 4072
Fragments arranged by size:
4072 677 466 318
BbeI G_GCGC'C
Cuts at: 2136 2349 2616 2136
Size: 213 267 5053
Fragments arranged by size:
5053 267 213
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2440 928
Size: 971 541 4021
Fragments arranged by size:
4021 971 541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2312 2346 2532 2566 3724 3758 1903
Size: 34 375 34 186 34 1158 34 3678
Fragments arranged by size:
3678 1158 375 186 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 4144 136
Size: 107 122 71 631 605 635 1837 1525
Fragments arranged by size:
1837 1525 635 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2547 5528 2547
Size: 2981 2552
BlpI GC'TnA_GC
Cuts at: 2067 2950 2067
Size: 883 4650
Bme1580I G_kGCm'C
Cuts at: 1790 2295 2670 3594 4750 1790
Size: 505 375 924 1156 2573
Fragments arranged by size:
2573 1156 924 505 375
BmgBI CAC'GTC
Cuts at: 1318 2026 1318
Size: 708 4825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 4222 442
Size: 416 53 83 399 24 1847 958 1753
Fragments arranged by size:
1847 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4193 1239
Size: 2954 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2321 3658 4523 4821 2249
Size: 72 1337 865 298 2961
Fragments arranged by size:
2961 1337 865 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4196 882
Size: 617 2697 2219
Fragments arranged by size:
2697 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2319 2346 2613
Size: 53 83 186 936 600 186 27 267
Cuts at: 2613 3340 3722 4357 275
Size: 727 382 635 1451
Fragments arranged by size:
1451 936 727 635 600 382 267 186
186 83 53 27
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 1178
Size: 30 165 30 1113 30 4165
Fragments arranged by size:
4165 1113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 1186
Size: 1066 4467
BseYI C'CCAG_C
Cuts at: 2587 2684 2735 2793 4736 5232 2587
Size: 97 51 58 1943 496 2888
Fragments arranged by size:
2888 1943 496 97 58 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 4357
Fragments arranged by size:
4357 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2171 2240 2418 2424 2466 2601
Size: 275 186 170 69 178 6 42 135
Cuts at: 2601 2652 2983 3744 4772 1540
Size: 51 331 761 1028 2301
Fragments arranged by size:
2301 1028 761 331 275 186 178 170
135 69 51 42 6
BsiHKAI G_wGCw'C
Cuts at: 2220 2328 3594 3679 4750 2220
Size: 108 1266 85 1071 3003
Fragments arranged by size:
3003 1266 1071 108 85
BsiWI C'GTAC_G
Cuts at: 2574 2574
Size: 5533
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2220 2295 2328 2534 2670 2852
Size: 399 278 152 75 33 206 136 182
Cuts at: 2852 3594 3679 4750 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 278 206 182 152
136 85 75 33
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 3422 5152 3422
Size: 1730 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2888 844
Size: 2044 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2758 3420 1394
Size: 429 935 662 3507
Fragments arranged by size:
3507 935 662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2235 4177 4364 1700
Size: 110 204 221 1942 187 2869
Fragments arranged by size:
2869 1942 221 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2017
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2547 2863 3230 3615 3632 5084 5170 5182
Size: 575 316 367 385 17 1452 86 12
Cuts at: 5182 5528 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 575 385 367 346 316 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2240 2418 2499 2601 2620 2811 513
Size: 552 1175 178 81 102 19 191 3235
Fragments arranged by size:
3235 1175 552 191 178 102 81 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 3049 3843 3863 3049
Size: 794 20 4719
Fragments arranged by size:
4719 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2168 2237 2415
Size: 54 1425 50 275 18 338 69 178
Cuts at: 2415 2421 2463 2649 2980 3869 5498 8
Size: 6 42 186 331 889 1629 43
Fragments arranged by size:
1629 1425 889 338 331 275 186 178
69 54 50 43 42 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2168 2237 2415 2421 2463 2649 2980
Size: 275 356 69 178 6 42 186 331
Cuts at: 2980 1537
Size: 4090
Fragments arranged by size:
4090 356 331 275 186 178 69 42
6
EarI CTCTTCn'nnn_
Cuts at: 1353 3463 1353
Size: 2110 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 4120 4494 4640 387
Size: 1064 138 741 1790 374 146 1280
Fragments arranged by size:
1790 1280 1064 741 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3596 4193 4980 5248 1239
Size: 393 1964 597 787 268 1524
Fragments arranged by size:
1964 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5533
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 5533
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2128 2136 2349 2616 4680 1678
Size: 450 8 213 267 2064 2531
Fragments arranged by size:
2531 2064 450 267 213 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2537 2548 2569 4174
Size: 32 135 32 944 21 11 21 1605
Cuts at: 4174 4206 4248 4280 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1605 944 135 42 32 32 32
32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KasI G'GCGC_C
Cuts at: 2132 2345 2612 2132
Size: 213 267 5053
Fragments arranged by size:
5053 267 213
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 2443 2577 5409 2443
Size: 134 2832 2567
Fragments arranged by size:
2832 2567 134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 4647 4831 1305
Size: 834 2508 184 2007
Fragments arranged by size:
2508 2007 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3711
Fragments arranged by size:
3711 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 3491 3850 4009 518
Size: 648 1041 1284 359 159 2042
Fragments arranged by size:
2042 1284 1041 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2242 2420 2432 2468 2480 2501 2603
Size: 465 171 178 12 36 12 21 102
Cuts at: 2603 2645 2797 2813 2949 3626 4774 5019 1606
Size: 42 152 16 136 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 465 245 178 171 152
136 102 42 36 21 16 12 12
NarI GG'CG_CC
Cuts at: 2133 2346 2613 2133
Size: 213 267 5053
Fragments arranged by size:
5053 267 213
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4981
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2415 2421 2649 2980 2415
Size: 6 228 331 4968
Fragments arranged by size:
4968 331 228 6
NspI r_CATG'y
Cuts at: 1132 1229 2891 4436 1132
Size: 97 1662 1545 2229
Fragments arranged by size:
2229 1662 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2644 2726 2751 2885 830
Size: 1814 82 25 134 3478
Fragments arranged by size:
3478 1814 134 82 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2797 2797
Size: 5533
SacII CC_GC'GG
Cuts at: 2243 2421 2502 2604 2814 2243
Size: 178 81 102 210 4962
Fragments arranged by size:
4962 210 178 102 81
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2640 2722 2747 2881 4016 4697 4888
Size: 219 1595 82 25 134 1135 681 191
Cuts at: 4888 826
Size: 1471
Fragments arranged by size:
1595 1471 1135 681 219 191 134 82
25
SfoI GGC'GCC
Cuts at: 2134 2347 2614 2134
Size: 213 267 5053
Fragments arranged by size:
5053 267 213
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2691 2700 2743 2860 1872
Size: 819 9 43 117 4545
Fragments arranged by size:
4545 819 117 43 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2336 3637 4538 4800 820
Size: 197 926 321 72 1301 901 262 1553
Fragments arranged by size:
1553 1301 926 901 321 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SrfI GCCC'GGGC
Cuts at: 2691 2691
Size: 5533
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2972 513
Size: 552 1907 3074
Fragments arranged by size:
3074 1907 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3560 3577 3730 3915 4360 325
Size: 1255 1980 17 153 185 445 1498
Fragments arranged by size:
1980 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3779 5491 96
Size: 274 80 33 51 1443 1802 1712 138
Fragments arranged by size:
1802 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 2024 1316
Size: 708 4825
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2689 2698 2741 2858 1870
Size: 819 9 43 117 4545
Fragments arranged by size:
4545 819 117 43 9
XmnI GAAnn'nnTTC
Cuts at: 2045 3662 2045
Size: 1617 3916
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SwaI XcmI