Restriction map for
pFN21AB9650
(Circular) (Six-base) MAPSORT of: pFN21AB9650.seq Check: 9277 from: 1 to: 5650
pFN21AB9650 5650 bp
With 182 enzymes: SgfI *
March 28, 2012 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 5097 5365 1632
Size: 2081 1384 268 1917
Fragments arranged by size:
2081 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 2245 2714 3393 4965 2245
Size: 469 679 1572 2930
Fragments arranged by size:
2930 1572 679 469
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2959 3185 3337 5446 1493
Size: 1466 226 152 2109 1697
Fragments arranged by size:
2109 1697 1466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2063 2110 2417 2489 2548 2592
Size: 73 113 7 47 307 72 59 44
Cuts at: 2592 2842 2882 2975 1870
Size: 250 40 93 4545
Fragments arranged by size:
4545 307 250 113 93 73 72 59
47 44 40 7
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2356 2485 2913 2971 4426
Size: 325 217 627 569 129 428 58 1455
Cuts at: 4426 618
Size: 1842
Fragments arranged by size:
1842 1455 627 569 428 325 217 129
58
BanII G_rGCy'C
Cuts at: 1391 2094 2969 1391
Size: 703 875 4072
Fragments arranged by size:
4072 875 703
BbeI G_GCGC'C
Cuts at: 2360 2489 2917 2360
Size: 129 428 5093
Fragments arranged by size:
5093 428 129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2511 928
Size: 971 612 4067
Fragments arranged by size:
4067 971 612
BbvCI CC'TCA_GC
Cuts at: 2715 2715
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2442 2793 4261
Size: 107 122 71 631 605 770 351 1468
Cuts at: 4261 136
Size: 1525
Fragments arranged by size:
1525 1468 770 631 605 351 122 107
71
BglII A'GATC_T
Cuts at: 2211 5645 2211
Size: 3434 2216
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2849 3711 4867 1790
Size: 1059 862 1156 2573
Fragments arranged by size:
2573 1156 1059 862
BmgBI CAC'GTC
Cuts at: 1318 2850 1318
Size: 1532 4118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4310 1239
Size: 3071 2579
Bpu10I CC'TnA_GC
Cuts at: 2715 2715
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2396 3775 4640 4938 2282
Size: 114 1379 865 298 2994
Fragments arranged by size:
2994 1379 865 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2537 4313 882
Size: 617 1038 1776 2219
Fragments arranged by size:
2219 1776 1038 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2357 2486 2741 2914
Size: 53 83 186 936 824 129 255 173
Cuts at: 2914 3457 3839 4474 275
Size: 543 382 635 1451
Fragments arranged by size:
1451 936 824 635 543 382 255 186
173 129 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2531 2693 2708 2711 2714 2744 2765
Size: 904 441 162 15 3 3 30 21
Cuts at: 2765 1186
Size: 4071
Fragments arranged by size:
4071 904 441 162 30 21 15 3
3
BseYI C'CCAG_C
Cuts at: 2758 4853 5349 2758
Size: 2095 496 3059
Fragments arranged by size:
3059 2095 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 2860 1163
Size: 573 294 830 3953
Fragments arranged by size:
3953 830 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 2336 2640 2793 3100 3861
Size: 275 186 72 263 304 153 307 761
Cuts at: 3861 4889 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 307 304 275 263 186
153 72
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 2141 3161 3254 2141
Size: 1020 93 4537
Fragments arranged by size:
4537 1020 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2726 2726
Size: 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2849 2969 3711 3796 4867 1391
Size: 399 304 755 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 755 742 399 304 120 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 3005 844
Size: 1994 167 3489
Fragments arranged by size:
3489 1994 167
BsrBI CCG'CTC
Cuts at: 1394 1823 2115 2287 2591 3537 1394
Size: 429 292 172 304 946 3507
Fragments arranged by size:
3507 946 429 304 292 172
BsrDI GCAATG_nn'
Cuts at: 65 1248 4145 4319 65
Size: 1183 2897 174 1396
Fragments arranged by size:
2897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 2331 2406 2792 4294 4481 1700
Size: 110 258 263 75 386 1502 187 2869
Fragments arranged by size:
2869 1502 386 263 258 187 110 75
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 2084 2608 1925
Size: 159 524 4967
Fragments arranged by size:
4967 524 159
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2245 794
Size: 1451 4199
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 2887 1184
Size: 1703 3947
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2224
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2211 2262 2980 3347 3732 3749 5201 5287
Size: 239 51 718 367 385 17 1452 86
Cuts at: 5287 5299 5645 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 239 86
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2437 513
Size: 552 939 433 3726
Fragments arranged by size:
3726 939 552 433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2911 487
Size: 683 56 1685 3226
Fragments arranged by size:
3226 1685 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2740 4657 809
Size: 507 1424 1917 1802
Fragments arranged by size:
1917 1802 1424 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2116 2333
Size: 54 1425 50 275 18 240 46 217
Cuts at: 2333 2434 2637 2790 3097 3986 5615 8
Size: 101 203 153 307 889 1629 43
Fragments arranged by size:
1629 1425 889 307 275 240 217 203
153 101 54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 2333 2637 2790 3097 1537
Size: 275 258 263 304 153 307 4090
Fragments arranged by size:
4090 307 304 275 263 258 153
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 2721 3580 1353
Size: 1332 36 859 3423
Fragments arranged by size:
3423 1332 859 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2185 2297 2324 4237 4611 4757
Size: 1064 138 596 112 27 1913 374 146
Cuts at: 4757 387
Size: 1280
Fragments arranged by size:
1913 1280 1064 596 374 146 138 112
27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3713 4310 5097 5365 1239
Size: 393 2081 597 787 268 1524
Fragments arranged by size:
2081 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2060 2462 2060
Size: 402 5248
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FseI GG_CCGG'CC
Cuts at: 2072 2796 2072
Size: 724 4926
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 2224 2360 2489 2811 2917 4797 1678
Size: 546 136 129 322 106 1880 2531
Fragments arranged by size:
2531 1880 546 322 136 129 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373
Size: 32 135 32 2719 32 42 32 2626
Fragments arranged by size:
2719 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KasI G'GCGC_C
Cuts at: 2356 2485 2913 2356
Size: 129 428 5093
Fragments arranged by size:
5093 428 129
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2649 4764 4948 1305
Size: 1344 2115 184 2007
Fragments arranged by size:
2115 2007 1344 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3828
Fragments arranged by size:
3828 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3608 3967 4126 518
Size: 648 2442 359 159 2042
Fragments arranged by size:
2442 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2235 2242 2428 2439 2456 2711 3066
Size: 411 218 7 186 11 17 255 355
Cuts at: 3066 3743 4891 5136 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 411 355 255 245 218
186 17 11 7
NaeI GCC'GGC
Cuts at: 2070 2408 2794 2070
Size: 338 386 4926
Fragments arranged by size:
4926 386 338
NarI GG'CG_CC
Cuts at: 2357 2486 2914 2357
Size: 129 428 5093
Fragments arranged by size:
5093 428 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4159
Fragments arranged by size:
4159 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NgoMIV G'CCGG_C
Cuts at: 2068 2406 2792 2068
Size: 338 386 4926
Fragments arranged by size:
4926 386 338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 2637 3097 2637
Size: 460 5190
NruI TCG'CGA
Cuts at: 2820 2820
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2200 3008 4553 1132
Size: 97 971 808 1545 2229
Fragments arranged by size:
2229 1545 971 808 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5650
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2060 2060
Size: 5650
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2250 2807 2835 3002 830
Size: 1420 557 28 167 3478
Fragments arranged by size:
3478 1420 557 167 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2242 2428 2242
Size: 186 5464
SacII CC_GC'GG
Cuts at: 2440 2440
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SanDI GG'GwC_CC
Cuts at: 2060 2060
Size: 5650
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SexAI A'CCwGG_T
Cuts at: 2934 2934
Size: 5650
SfcI C'TryA_G
Cuts at: 826 1045 2246 2803 2831 2998 4133 4814 5005
Size: 219 1201 557 28 167 1135 681 191
Cuts at: 5005 826
Size: 1471
Fragments arranged by size:
1471 1201 1135 681 557 219 191 167
28
SfiI GGCCn_nnn'nGGCC
Cuts at: 2442 2793 2442
Size: 351 5299
SfoI GGC'GCC
Cuts at: 2358 2487 2915 2358
Size: 129 428 5093
Fragments arranged by size:
5093 428 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2058 2065 2419 2550 2977 1872
Size: 186 7 354 131 427 4545
Fragments arranged by size:
4545 427 354 186 131 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 2411 3754 4655 4917 820
Size: 197 926 354 114 1343 901 262 1553
Fragments arranged by size:
1553 1343 926 901 354 262 197 114
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SrfI GCCC'GGGC
Cuts at: 2419 2419
Size: 5650
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 2614 3089 513
Size: 552 939 360 250 475 3074
Fragments arranged by size:
3074 939 552 475 360 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3677 3694 3847 4032 4477 325
Size: 1255 2097 17 153 185 445 1498
Fragments arranged by size:
2097 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3896 5608 96
Size: 274 80 33 51 1443 1919 1712 138
Fragments arranged by size:
1919 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 2665 1316
Size: 1349 4301
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2056 2063 2417 2548 2975 1870
Size: 186 7 354 131 427 4545
Fragments arranged by size:
4545 427 354 186 131 7
XmnI GAAnn'nnTTC
Cuts at: 2141 3779 2141
Size: 1638 4012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FspAI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SapI SwaI XcmI