Restriction map for
pFN21AB9648
(Circular) (Six-base) MAPSORT of: pFN21AB9648.seq Check: 6660 from: 1 to: 5878
pFN21AB9648 5878 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3941 5325 5593 1632
Size: 2309 1384 268 1917
Fragments arranged by size:
2309 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 4777 5754 1203
Size: 3574 977 1327
Fragments arranged by size:
3574 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3062 1184
Size: 1878 4000
AlwNI CAG_nnn'CTG
Cuts at: 2512 3621 5193 2512
Size: 1109 1572 3197
Fragments arranged by size:
3197 1572 1109
ApaI G_GGCC'C
Cuts at: 2078 2078
Size: 5878
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 3187 3413 3565 5674 1493
Size: 1694 226 152 2109 1697
Fragments arranged by size:
2109 1697 1694 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2653 2815 3014 3203 1870
Size: 73 128 582 162 199 189 4545
Fragments arranged by size:
4545 582 199 189 162 128 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2132 2573 2840 2930
Size: 325 217 627 261 84 441 267 90
Cuts at: 2930 3004 3160 3199 4654 618
Size: 74 156 39 1455 1842
Fragments arranged by size:
1842 1455 627 441 325 267 261 217
156 90 84 74 39
BanII G_rGCy'C
Cuts at: 1391 2078 2427 2522 2951 2970 3197 1391
Size: 687 349 95 429 19 227 4072
Fragments arranged by size:
4072 687 429 349 227 95 19
BbeI G_GCGC'C
Cuts at: 2052 2136 2577 2844 2934 3008 2052
Size: 84 441 267 90 74 4922
Fragments arranged by size:
4922 441 267 90 84 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 3033 1548
Size: 1485 4393
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2526 2897 4489
Size: 107 122 71 631 605 854 371 1592
Cuts at: 4489 136
Size: 1525
Fragments arranged by size:
1592 1525 854 631 605 371 122 107
71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 2078 3014 3165 3939 5095 1790
Size: 288 936 151 774 1156 2573
Fragments arranged by size:
2573 1156 936 774 288 151
BmgBI CAC'GTC
Cuts at: 1318 2554 1318
Size: 1236 4642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4567 442
Size: 416 53 83 399 24 2192 958 1753
Fragments arranged by size:
2192 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 4538 1239
Size: 1295 2004 2579
Fragments arranged by size:
2579 2004 1295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 4003 4868 5166 2378
Size: 1625 865 298 3090
Fragments arranged by size:
3090 1625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 4541 882
Size: 617 519 2523 2219
Fragments arranged by size:
2523 2219 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2133 2574 2841
Size: 53 83 186 936 516 84 441 267
Cuts at: 2841 2931 3005 3685 4067 4702 275
Size: 90 74 680 382 635 1451
Fragments arranged by size:
1451 936 680 635 516 441 382 267
186 90 84 83 74 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5653
Fragments arranged by size:
5653 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5878
BseYI C'CCAG_C
Cuts at: 2562 2944 2969 2989 5081 5577 2562
Size: 382 25 20 2092 496 2863
Fragments arranged by size:
2863 2092 496 382 25 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 2318 3092 1163
Size: 573 282 300 774 3949
Fragments arranged by size:
3949 774 573 300 282
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2300 2369 2436 2499 2610 2850
Size: 275 186 299 69 67 63 111 240
Cuts at: 2850 2892 2919 3090 3328 4089 5117 1540
Size: 42 27 171 238 761 1028 2301
Fragments arranged by size:
2301 1028 761 299 275 240 238 186
171 111 69 67 63 42 27
BsiHKAI G_wGCw'C
Cuts at: 2349 3939 4024 5095 2349
Size: 1590 85 1071 3132
Fragments arranged by size:
3132 1590 1071 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2349 2427 2522 2951 2970 3014
Size: 399 288 271 78 95 429 19 44
Cuts at: 3014 3165 3197 3939 4024 5095 1391
Size: 151 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 288 271 151
95 85 78 44 32 19
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2040 3030 3233 844
Size: 1196 990 203 3489
Fragments arranged by size:
3489 1196 990 203
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2364 2417 2435 3053 3102 4522 4709
Size: 110 554 53 18 618 49 1420 187
Cuts at: 4709 1700
Size: 2869
Fragments arranged by size:
2869 1420 618 554 187 110 53 49
18
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3967
BssHII G'CGCG_C
Cuts at: 1925 2326 2328 2667 2760 2874 2940 1925
Size: 401 2 339 93 114 66 4863
Fragments arranged by size:
4863 401 339 114 93 66 2
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2740 3115 794
Size: 1946 375 3557
Fragments arranged by size:
3557 1946 375
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2473
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873
Size: 1236 367 385 17 1452 86 12 346
Cuts at: 5873 1972
Size: 1977
Fragments arranged by size:
1977 1452 1236 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2191 2466 2499 2610 2844 2892 3090
Size: 552 1126 275 33 111 234 48 198
Cuts at: 3090 513
Size: 3301
Fragments arranged by size:
3301 1126 552 275 234 198 111 48
33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 3394 4188 4208 3394
Size: 794 20 5064
Fragments arranged by size:
5064 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2297 2366 2415
Size: 54 1425 50 275 18 467 69 49
Cuts at: 2415 2433 2496 2607 2613 2847 2889 2916 3087
Size: 18 63 111 6 234 42 27 171
Cuts at: 3087 3325 4214 5843 8
Size: 238 889 1629 43
Fragments arranged by size:
1629 1425 889 467 275 238 234 171
111 69 63 54 50 49 43 42
27 18 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2297 2366 2433 2496 2607 2847 2889
Size: 275 485 69 67 63 111 240 42
Cuts at: 2889 2916 3087 3325 1537
Size: 27 171 238 4090
Fragments arranged by size:
4090 485 275 240 238 171 111 69
67 63 42 27
EarI CTCTTCn'nnn_
Cuts at: 1353 3808 1353
Size: 2455 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2118 4465 4839 4985 387
Size: 1064 138 529 2347 374 146 1280
Fragments arranged by size:
2347 1280 1064 529 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 3941 4538 5325 5593 1239
Size: 393 902 1407 597 787 268 1524
Fragments arranged by size:
1524 1407 902 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2056 2075 3019 2056
Size: 19 944 4915
Fragments arranged by size:
4915 944 19
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 2052 2136 2257 2512 2577 2844 2934 3008
Size: 374 84 121 255 65 267 90 74
Cuts at: 3008 5025 1678
Size: 2017 2531
Fragments arranged by size:
2531 2017 374 267 255 121 90 84
74 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2393 2425 4519
Size: 32 135 32 449 32 340 32 2094
Cuts at: 4519 4551 4593 4625 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2094 449 340 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2557 3222 3468 669
Size: 988 281 619 665 246 3079
Fragments arranged by size:
3079 988 665 619 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KasI G'GCGC_C
Cuts at: 2048 2132 2573 2840 2930 3004 2048
Size: 84 441 267 90 74 4922
Fragments arranged by size:
4922 441 267 90 84 74
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2808 2943 4992 5176 1305
Size: 1503 135 2049 184 2007
Fragments arranged by size:
2049 2007 1503 184 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4056
Fragments arranged by size:
4056 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2471 3836 4195 4354 518
Size: 648 852 453 1365 359 159 2042
Fragments arranged by size:
2042 1365 852 648 453 359 159
MspA1I CmG'CkG
Cuts at: 1606 2193 2200 2612 2635 2846 2864 2973 3086
Size: 587 7 412 23 211 18 109 113
Cuts at: 3086 3092 3294 3971 5119 5364 1606
Size: 6 202 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 587 412 245 211 202
113 109 23 18 7 6
NaeI GCC'GGC
Cuts at: 2419 2437 3055 3104 2419
Size: 18 618 49 5193
Fragments arranged by size:
5193 618 49 18
NarI GG'CG_CC
Cuts at: 2049 2133 2574 2841 2931 3005 2049
Size: 84 441 267 90 74 4922
Fragments arranged by size:
4922 441 267 90 84 74
NcoI C'CATG_G
Cuts at: 513 1065 2466 513
Size: 552 1401 3925
Fragments arranged by size:
3925 1401 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NgoMIV G'CCGG_C
Cuts at: 2417 2435 3053 3102 2417
Size: 18 618 49 5193
Fragments arranged by size:
5193 618 49 18
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 2607 2847 2916 3087 3325 2607
Size: 240 69 171 238 5160
Fragments arranged by size:
5160 240 238 171 69
NspI r_CATG'y
Cuts at: 1132 1229 3043 3236 4781 1132
Size: 97 1814 193 1545 2229
Fragments arranged by size:
2229 1814 1545 193 97
PasI CC'CwG_GG
Cuts at: 1348 2935 1348
Size: 1587 4291
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2512 1108
Size: 1404 4474
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3019 3019
Size: 5878
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PspOMI G'GGCC_C
Cuts at: 2074 2074
Size: 5878
PstI C_TGCA'G
Cuts at: 830 2769 3230 830
Size: 1939 461 3478
Fragments arranged by size:
3478 1939 461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2635 2635
Size: 5878
SacII CC_GC'GG
Cuts at: 2194 2613 2847 3093 2194
Size: 419 234 246 4979
Fragments arranged by size:
4979 419 246 234
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 2408 4126 5838 2408
Size: 1718 1712 2448
Fragments arranged by size:
2448 1718 1712
SfcI C'TryA_G
Cuts at: 826 1045 2460 2765 3226 4361 5042 5233 826
Size: 219 1415 305 461 1135 681 191 1471
Fragments arranged by size:
1471 1415 1135 681 461 305 219 191
SfoI GGC'GCC
Cuts at: 2050 2134 2575 2842 2932 3006 2050
Size: 84 441 267 90 74 4922
Fragments arranged by size:
4922 441 267 90 84 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 3053 1700
Size: 1353 4525
SmaI CCC'GGG
Cuts at: 1872 2655 3016 3205 1872
Size: 783 361 189 4545
Fragments arranged by size:
4545 783 361 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3982 4883 5145 820
Size: 197 926 450 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2136 2466 3317 513
Size: 552 1071 330 851 3074
Fragments arranged by size:
3074 1071 851 552 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 3905 3922 4075 4260 4705 325
Size: 1255 462 1863 17 153 185 445 1498
Fragments arranged by size:
1863 1498 1255 462 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2406 2769
Size: 274 80 33 51 1443 30 399 363
Cuts at: 2769 3042 4124 5836 96
Size: 273 1082 1712 138
Fragments arranged by size:
1712 1443 1082 399 363 274 273 138
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 2153 1316
Size: 837 5041
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 2653 3014 3203 1870
Size: 783 361 189 4545
Fragments arranged by size:
4545 783 361 189
XmnI GAAnn'nnTTC
Cuts at: 2174 4007 2174
Size: 1833 4045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SrfI SwaI XcmI