Restriction map for
pFN21AB9647
(Circular) (Six-base) MAPSORT of: pFN21AB9647.seq Check: 1955 from: 1 to: 6643
pFN21AB9647 6643 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4453 278
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3136 3472 3892 3986 1937
Size: 1199 336 420 94 4594
Fragments arranged by size:
4594 1199 420 336 94
Acc65I G'GTAC_C
Cuts at: 3964 3964
Size: 6643
AclI AA'CG_TT
Cuts at: 1563 4770 5143 1563
Size: 3207 373 3063
Fragments arranged by size:
3207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2673 2841 3093 3681 3945 4706 6090 6358
Size: 1041 168 252 588 264 761 1384 268
Cuts at: 6358 1632
Size: 1917
Fragments arranged by size:
1917 1384 1041 761 588 268 264 252
168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6446
AflIII A'CryG_T
Cuts at: 1203 5542 6519 1203
Size: 4339 977 1327
Fragments arranged by size:
4339 1327 977
AgeI A'CCGG_T
Cuts at: 5474 5474
Size: 6643
AhdI GACnn_n'nnGTC
Cuts at: 5372 5372
Size: 6643
AleI CACnn'nnGTG
Cuts at: 1166 2670 2838 1166
Size: 1504 168 4971
Fragments arranged by size:
4971 1504 168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6643
AlwNI CAG_nnn'CTG
Cuts at: 4386 5958 4386
Size: 1572 5071
ApaLI G'TGCA_C
Cuts at: 2021 4700 5856 2021
Size: 2679 1156 2808
Fragments arranged by size:
2808 2679 1156
ApoI r'AATT_y
Cuts at: 1493 2122 2226 2242 2391 2585 2727 2895 3315
Size: 629 104 16 149 194 142 168 420
Cuts at: 3315 3483 3567 3915 3952 4178 4330 6439 1493
Size: 168 84 348 37 226 152 2109 1697
Fragments arranged by size:
2109 1697 629 420 348 226 194 168
168 152 149 142 104 84 37 16
AseI AT'TA_AT
Cuts at: 160 1032 5197 160
Size: 872 4165 1606
Fragments arranged by size:
4165 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 3968 1870
Size: 73 2025 4545
Fragments arranged by size:
4545 2025 73
BamHI G'GATC_C
Cuts at: 3973 4340 3973
Size: 367 6276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3964 5419 618
Size: 325 217 627 2177 1455 1842
Fragments arranged by size:
2177 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3962 1391
Size: 2571 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 3870 928
Size: 971 963 1008 3701
Fragments arranged by size:
3701 1008 971 963
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4834 4868 1903
Size: 34 2897 34 3678
Fragments arranged by size:
3678 2897 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4535 5745 4535
Size: 1210 5433
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5254 136
Size: 107 122 71 631 605 3582 1525
Fragments arranged by size:
3582 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6638 6638
Size: 6643
BlpI GC'TnA_GC
Cuts at: 4060 4060
Size: 6643
Bme1580I G_kGCm'C
Cuts at: 1790 2025 3561 4704 5860 1790
Size: 235 1536 1143 1156 2573
Fragments arranged by size:
2573 1536 1156 1143 235
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4374 5332 442
Size: 416 53 83 399 24 2957 958 1753
Fragments arranged by size:
2957 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2340 2757 2925 3177 3261 3513 3765 3849
Size: 1101 417 168 252 84 252 252 84
Cuts at: 3849 5303 1239
Size: 1454 2579
Fragments arranged by size:
2579 1454 1101 417 252 252 252 168
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4768 5633 5931 4768
Size: 865 298 5480
Fragments arranged by size:
5480 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5306 882
Size: 617 3807 2219
Fragments arranged by size:
3807 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4434 493
Size: 711 3230 2702
Fragments arranged by size:
3230 2702 711
BsaBI GATnn'nnATC
Cuts at: 6637 6637
Size: 6643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4450 4832 5467 275
Size: 53 83 186 936 2917 382 635 1451
Fragments arranged by size:
2917 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5076 5474 5748 5895 6306 5076
Size: 398 274 147 411 5413
Fragments arranged by size:
5413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6418
Fragments arranged by size:
6418 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6643
BseYI C'CCAG_C
Cuts at: 3554 5846 6342 3554
Size: 2292 496 3855
Fragments arranged by size:
3855 2292 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6070
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4093 4854 5882 1540
Size: 275 186 2092 761 1028 2301
Fragments arranged by size:
2301 2092 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 4704 4789 5860 2025
Size: 2679 85 1071 2808
Fragments arranged by size:
2808 2679 1071 85
BsmI GAATG_Cn'
Cuts at: 4154 4247 4154
Size: 93 6550
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 3561 3962 4704 4789 5860 1391
Size: 399 235 1536 401 742 85 1071 2174
Fragments arranged by size:
2174 1536 1071 742 401 399 235 85
BspEI T'CCGG_A
Cuts at: 6306 6306
Size: 6643
BspHI T'CATG_A
Cuts at: 4532 6262 4532
Size: 1730 4913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3998 844
Size: 3154 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4530 1394
Size: 429 2707 3507
Fragments arranged by size:
3507 2707 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5138 5312 65
Size: 1183 3890 174 1396
Fragments arranged by size:
3890 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5287 5474 1700
Size: 110 3477 187 2869
Fragments arranged by size:
3477 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3577 3934 96
Size: 3481 357 2805
Fragments arranged by size:
3481 2805 357
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6643
BssSI C'ACGA_G
Cuts at: 4703 5715 4703
Size: 1012 5631
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6643
BstBI TT'CG_AA
Cuts at: 5447 5447
Size: 6643
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstYI r'GATC_y
Cuts at: 1972 3973 4340 4725 4742 6194 6280 6292 6638
Size: 2001 367 385 17 1452 86 12 346
Cuts at: 6638 1972
Size: 1977
Fragments arranged by size:
2001 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3137 3473 3137
Size: 336 6307
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5904
Fragments arranged by size:
5904 683 56
BtsI GCAGTG_nn'
Cuts at: 4159 4953 4973 4159
Size: 794 20 5829
Fragments arranged by size:
5829 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 2121 3031 3199 3947 2121
Size: 910 168 748 4817
Fragments arranged by size:
4817 910 748 168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5650 809
Size: 507 4334 1802
Fragments arranged by size:
4334 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4090 4979 6608
Size: 54 1425 50 275 18 2260 889 1629
Cuts at: 6608 8
Size: 43
Fragments arranged by size:
2260 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4090 1537
Size: 275 2278 4090
Fragments arranged by size:
4090 2278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2649 2817 2985 3069 3657 3921 4573 1353
Size: 1296 168 168 84 588 264 652 3423
Fragments arranged by size:
3423 1296 652 588 264 168 168 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5230 5604 5750 387
Size: 1064 138 3641 374 146 1280
Fragments arranged by size:
3641 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2673 2757 2841 2925 3093 3177
Size: 393 708 333 84 84 84 168 84
Cuts at: 3177 3261 3513 3681 3765 3849 3945 4706 5303
Size: 84 252 168 84 84 96 761 597
Cuts at: 5303 6090 6358 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 761 708 597 393 333 268
252 168 168 96 84 84 84 84
84 84 84
EcoRI G'AATT_C
Cuts at: 2226 2242 2391 2727 2895 3315 3915 3952 4330
Size: 16 149 336 168 420 600 37 378
Cuts at: 4330 2226
Size: 4539
Fragments arranged by size:
4539 600 420 378 336 168 149 37
16
FspI TGC'GCA
Cuts at: 5149 5149
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 5790 1678
Size: 4112 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3447 3479 5284 5316 5358
Size: 32 135 32 1875 32 1805 32 42
Cuts at: 5358 5390 1373
Size: 32 2626
Fragments arranged by size:
2626 1875 1805 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3987 4233 669
Size: 988 281 2049 246 3079
Fragments arranged by size:
3079 2049 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2688 2856 3024 3108 3192 3276 3444 3528
Size: 1940 168 168 84 84 84 168 84
Cuts at: 3528 3780 3864 748
Size: 252 84 3527
Fragments arranged by size:
3527 1940 252 168 168 168 84 84
84 84 84
HpaI GTT'AAC
Cuts at: 4233 4233
Size: 6643
KpnI G_GTAC'C
Cuts at: 3968 3968
Size: 6643
MfeI C'AATT_G
Cuts at: 4242 4242
Size: 6643
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5757 5941 1305
Size: 4452 184 2007
Fragments arranged by size:
4452 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4821
Fragments arranged by size:
4821 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2670 2838 4601 4960 5119 518
Size: 648 1504 168 1763 359 159 2042
Fragments arranged by size:
2042 1763 1504 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 4059 4736 5884 6129 1606
Size: 2453 677 1148 245 2120
Fragments arranged by size:
2453 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6091
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6643
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 4090 4090
Size: 6643
NspI r_CATG'y
Cuts at: 1132 1229 2200 3858 4001 5546 1132
Size: 97 971 1658 143 1545 2229
Fragments arranged by size:
2229 1658 1545 971 143 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6643
PciI A'CATG_T
Cuts at: 5542 5542
Size: 6643
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6643
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 6225 6257 4712
Size: 32 1481 32 5098
Fragments arranged by size:
5098 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6643
PsiI TTA'TAA
Cuts at: 2037 2694 2862 4213 2037
Size: 657 168 1351 4467
Fragments arranged by size:
4467 1351 657 168
PstI C_TGCA'G
Cuts at: 830 3995 830
Size: 3165 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6643
SalI G'TCGA_C
Cuts at: 1936 3985 1936
Size: 2049 4594
SbfI CC_TGCA'GG
Cuts at: 3995 3995
Size: 6643
ScaI AGT'ACT
Cuts at: 4891 6603 4891
Size: 1712 4931
SfcI C'TryA_G
Cuts at: 826 1045 3991 5126 5807 5998 826
Size: 219 2946 1135 681 191 1471
Fragments arranged by size:
2946 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6643
SmaI CCC'GGG
Cuts at: 1872 3970 1872
Size: 2098 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4747 5648 5910 820
Size: 197 926 2804 901 262 1553
Fragments arranged by size:
2804 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4434 493
Size: 3941 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6643
SphI G_CATG'C
Cuts at: 1132 2200 4001 1132
Size: 1068 1801 3774
Fragments arranged by size:
3774 1801 1068
SspI AAT'ATT
Cuts at: 5 52 2079 2681 4567 5
Size: 47 2027 602 1886 2081
Fragments arranged by size:
2081 2027 1886 602 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6643
StyI C'CwwG_G
Cuts at: 513 1065 4082 513
Size: 552 3017 3074
Fragments arranged by size:
3074 3017 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3276 4670 4687 4840 5025 5470 325
Size: 1255 1696 1394 17 153 185 445 1498
Fragments arranged by size:
1696 1498 1394 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3577 3934 4889
Size: 274 80 33 51 1443 1600 357 955
Cuts at: 4889 6601 96
Size: 1712 138
Fragments arranged by size:
1712 1600 1443 955 357 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6603 6635 6603
Size: 32 6611
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6643
XbaI T'CTAG_A
Cuts at: 3979 3979
Size: 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6643
XmaI C'CCGG_G
Cuts at: 1870 3968 1870
Size: 2098 4545
XmnI GAAnn'nnTTC
Cuts at: 2162 4772 2162
Size: 2610 4033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4451 276
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI