Restriction map for
pFN21AB9645
(Circular) (Six-base) MAPSORT of: pFN21AB9645.seq Check: 7456 from: 1 to: 7801
pFN21AB9645 7801 bp
With 182 enzymes: SgfI *
March 28, 2012 12:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2363 3658 2363
Size: 1295 6506
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2504 5611 278
Size: 53 83 186 936 968 3107 2468
Fragments arranged by size:
3107 2468 968 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5144 1937
Size: 3207 4594
Acc65I G'GTAC_C
Cuts at: 5122 5122
Size: 7801
AclI AA'CG_TT
Cuts at: 1563 5928 6301 1563
Size: 4365 373 3063
Fragments arranged by size:
4365 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 2810 2900 2921 3378 4837 4880 5864
Size: 454 724 90 21 457 1459 43 984
Cuts at: 5864 7248 7516 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1459 1384 984 724 457 454 268
90 43 21
AfeI AGC'GCT
Cuts at: 2744 4111 2744
Size: 1367 6434
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7604
AflIII A'CryG_T
Cuts at: 1203 3774 6700 7677 1203
Size: 2571 2926 977 1327
Fragments arranged by size:
2926 2571 1327 977
AgeI A'CCGG_T
Cuts at: 4510 6632 4510
Size: 2122 5679
AhdI GACnn_n'nnGTC
Cuts at: 4504 6530 4504
Size: 2026 5775
AleI CACnn'nnGTG
Cuts at: 1166 2642 2682 4241 1166
Size: 1476 40 1559 4726
Fragments arranged by size:
4726 1559 1476 40
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2490 4226 4272 1184
Size: 1306 1736 46 4713
Fragments arranged by size:
4713 1736 1306 46
AlwNI CAG_nnn'CTG
Cuts at: 2944 4695 5544 7116 2944
Size: 1751 849 1572 3629
Fragments arranged by size:
3629 1751 1572 849
ApaI G_GGCC'C
Cuts at: 3953 4637 4943 3953
Size: 684 306 6811
Fragments arranged by size:
6811 684 306
ApaLI G'TGCA_C
Cuts at: 2657 4649 5858 7014 2657
Size: 1992 1209 1156 3444
Fragments arranged by size:
3444 1992 1209 1156
ApoI r'AATT_y
Cuts at: 1493 3446 4742 5110 5336 5488 7597 1493
Size: 1953 1296 368 226 152 2109 1697
Fragments arranged by size:
2109 1953 1697 1296 368 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6355 160
Size: 872 5323 1606
Fragments arranged by size:
5323 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 3625 4585 5060 5126 1870
Size: 73 205 1477 960 475 66 4545
Fragments arranged by size:
4545 1477 960 475 205 73 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2413 2446 2413
Size: 33 7768
BamHI G'GATC_C
Cuts at: 2164 5131 5498 2164
Size: 2967 367 4467
Fragments arranged by size:
4467 2967 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2466 2594 2947 3071 3629
Size: 325 217 627 679 128 353 124 558
Cuts at: 3629 5122 6577 618
Size: 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 679 627 558 353 325
217 128 124
BanII G_rGCy'C
Cuts at: 1391 2330 2529 3456 3863 3953 4637 4674 4943
Size: 939 199 927 407 90 684 37 269
Cuts at: 4943 4973 5120 1391
Size: 30 147 4072
Fragments arranged by size:
4072 939 927 684 407 269 199 147
90 37 30
BbeI G_GCGC'C
Cuts at: 2598 2951 3075 2598
Size: 353 124 7324
Fragments arranged by size:
7324 353 124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2578 2720 3699 4968 928
Size: 971 679 142 979 1269 3761
Fragments arranged by size:
3761 1269 979 971 679 142
BbvCI CC'TCA_GC
Cuts at: 2834 2834
Size: 7801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5992 6026 1903
Size: 34 4055 34 3678
Fragments arranged by size:
4055 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5693 6903 5693
Size: 1210 6591
BclI T'GATC_A
Cuts at: 1548 2847 1548
Size: 1299 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3104 6412 136
Size: 107 122 71 631 605 1432 3308 1525
Fragments arranged by size:
3308 1525 1432 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7796 7796
Size: 7801
BlpI GC'TnA_GC
Cuts at: 2263 4106 5218 2263
Size: 1843 1112 4846
Fragments arranged by size:
4846 1843 1112
Bme1580I G_kGCm'C
Cuts at: 1790 2661 3189 3632 3953 4637 4653 4943 5862
Size: 871 528 443 321 684 16 290 919
Cuts at: 5862 7018 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 919 871 684 528 443 321
290 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7801
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2051 3975 4570
Size: 416 53 83 399 24 634 1924 595
Cuts at: 4570 4677 4955 5532 6490 442
Size: 107 278 577 958 1753
Fragments arranged by size:
1924 1753 958 634 595 577 416 399
278 107 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2126 1056
Size: 1070 6731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3877 3877
Size: 7801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 3295 3554 6461 1239
Size: 1156 900 259 2907 2579
Fragments arranged by size:
2907 2579 1156 900 259
Bpu10I CC'TnA_GC
Cuts at: 2523 2834 2964 2523
Size: 311 130 7360
Fragments arranged by size:
7360 311 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3509 5926 6791 7089 3101
Size: 408 2417 865 298 3813
Fragments arranged by size:
3813 2417 865 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3832 4592 6464 882
Size: 617 2333 760 1872 2219
Fragments arranged by size:
2333 2219 1872 760 617
BsaAI yAC'GTr
Cuts at: 493 1204 5592 493
Size: 711 4388 2702
Fragments arranged by size:
4388 2702 711
BsaBI GATnn'nnATC
Cuts at: 7795 7795
Size: 7801
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2501 2595 2948
Size: 53 83 186 936 549 419 94 353
Cuts at: 2948 3072 4424 5608 5990 6625 275
Size: 124 1352 1184 382 635 1451
Fragments arranged by size:
1451 1352 1184 936 635 549 419 382
353 186 124 94 83 53
BsaWI w'CCGG_w
Cuts at: 2607 4081 4510 4998 6234 6632 6906 7053 7464
Size: 1474 429 488 1236 398 274 147 411
Cuts at: 7464 2607
Size: 2944
Fragments arranged by size:
2944 1474 1236 488 429 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 2225 2255 2690
Size: 30 165 30 633 30 159 30 435
Cuts at: 2690 2720 4955 4985 1178
Size: 30 2235 30 3994
Fragments arranged by size:
3994 2235 633 435 165 159 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3437 3497 3803 3848 4502 4738 1186
Size: 2251 60 306 45 654 236 4249
Fragments arranged by size:
4249 2251 654 306 236 60 45
BseYI C'CCAG_C
Cuts at: 2393 2528 3455 3599 7004 7500 2393
Size: 135 927 144 3405 496 2694
Fragments arranged by size:
3405 2694 927 496 144 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2460 2567 3677 3686 3692 3716 5015
Size: 573 724 107 1110 9 6 24 1299
Cuts at: 5015 1163
Size: 3949
Fragments arranged by size:
3949 1299 1110 724 573 107 24 9
6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3654 4267 5251 6012 7040 1540
Size: 275 186 1653 613 984 761 1028 2301
Fragments arranged by size:
2301 1653 1028 984 761 613 275 186
BsiHKAI G_wGCw'C
Cuts at: 2330 2390 2661 3720 3863 4653 5862 5947 7018
Size: 60 271 1059 143 790 1209 85 1071
Cuts at: 7018 2330
Size: 3113
Fragments arranged by size:
3113 1209 1071 1059 790 271 143 85
60
BsiWI C'GTAC_G
Cuts at: 2631 3033 2631
Size: 402 7399
BsmI GAATG_Cn'
Cuts at: 5312 5405 5312
Size: 93 7708
BsmBI CGTCTCn'nnnn_
Cuts at: 2012 2191 3550 2012
Size: 179 1359 6263
Fragments arranged by size:
6263 1359 179
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2390 2529 2661 3189 3456 3632
Size: 399 540 60 139 132 528 267 176
Cuts at: 3632 3720 3863 3953 4637 4653 4674 4943 4973
Size: 88 143 90 684 16 21 269 30
Cuts at: 4973 5120 5862 5947 7018 1391
Size: 147 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 540 528 399 269
267 176 147 143 139 132 90 88
85 60 30 21 16
BspEI T'CCGG_A
Cuts at: 7464 7464
Size: 7801
BspHI T'CATG_A
Cuts at: 4125 5690 7420 4125
Size: 1565 1730 4506
Fragments arranged by size:
4506 1730 1565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2363 3483 3658 5156 844
Size: 1519 1120 175 1498 3489
Fragments arranged by size:
3489 1519 1498 1120 175
BsrBI CCG'CTC
Cuts at: 1394 1823 5688 1394
Size: 429 3865 3507
Fragments arranged by size:
3865 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2017 6296 6470 65
Size: 1183 769 4279 174 1396
Fragments arranged by size:
4279 1396 1183 769 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 3304 3646 4222 4510 4530 6445
Size: 110 274 1220 342 576 288 20 1915
Cuts at: 6445 6632 1700
Size: 187 2869
Fragments arranged by size:
2869 1915 1220 576 342 288 274 187
110 20
BsrGI T'GTAC_A
Cuts at: 96 4191 96
Size: 4095 3706
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7801
BssSI C'ACGA_G
Cuts at: 2383 5861 6873 2383
Size: 3478 1012 3311
Fragments arranged by size:
3478 3311 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2403 2619 3235 3664 794
Size: 1609 216 616 429 4931
Fragments arranged by size:
4931 1609 616 429 216
BstBI TT'CG_AA
Cuts at: 4913 6605 4913
Size: 1692 6109
BstEII G'GTnAC_C
Cuts at: 1184 3178 4036 1184
Size: 1994 858 4949
Fragments arranged by size:
4949 1994 858
BstYI r'GATC_y
Cuts at: 1972 2164 3111 3261 4864 5131 5498 5883 5900
Size: 192 947 150 1603 267 367 385 17
Cuts at: 5900 7352 7438 7450 7796 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1603 1452 947 385 367 346 267
192 150 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3954 3954
Size: 7801
BtgI C'CryG_G
Cuts at: 513 1065 3339 3549 3783 5047 513
Size: 552 2274 210 234 1264 3267
Fragments arranged by size:
3267 2274 1264 552 234 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4199 487
Size: 683 56 2973 4089
Fragments arranged by size:
4089 2973 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 2629 3641 5317 6111 6131 2177
Size: 452 1012 1676 794 20 3847
Fragments arranged by size:
3847 1676 1012 794 452 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7766
DraI TTT'AAA
Cuts at: 2277 5105 2277
Size: 2828 4973
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2837 4429 6808 809
Size: 507 1521 1592 2379 1802
Fragments arranged by size:
2379 1802 1592 1521 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2481 2979 3552
Size: 54 1425 50 275 18 651 498 573
Cuts at: 3552 3651 3867 4701 5248 6137 7766 8
Size: 99 216 834 547 889 1629 43
Fragments arranged by size:
1629 1425 889 834 651 573 547 498
275 216 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3651 5248 1537
Size: 275 1839 1597 4090
Fragments arranged by size:
4090 1839 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2146 2148 3249 3851 5731 1353
Size: 657 136 2 1101 602 1880 3423
Fragments arranged by size:
3423 1880 1101 657 602 136 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2320 3700 6388 6762 6908 387
Size: 1064 138 731 1380 2688 374 146 1280
Fragments arranged by size:
2688 1380 1280 1064 731 374 146 138
EcoICRI GAG'CTC
Cuts at: 2328 3861 2328
Size: 1533 6268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2395 2810 2900 2921 3295 3378
Size: 393 454 309 415 90 21 374 83
Cuts at: 3378 3554 4837 4880 5864 6461 7248 7516 1239
Size: 176 1283 43 984 597 787 268 1524
Fragments arranged by size:
1524 1283 984 787 597 454 415 393
374 309 268 176 90 83 43 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3479 3869 4142 3479
Size: 390 273 7138
Fragments arranged by size:
7138 390 273
EcoO109I rG'GnC_Cy
Cuts at: 2830 2941 3487 3949 3950 2830
Size: 111 546 462 1 6681
Fragments arranged by size:
6681 546 462 111 1
EcoRI G'AATT_C
Cuts at: 5110 5488 5110
Size: 378 7423
FspI TGC'GCA
Cuts at: 6307 6307
Size: 7801
HaeII r_GCGC'y
Cuts at: 1678 2598 2746 2936 2951 3075 4113 6948 1678
Size: 920 148 190 15 124 1038 2835 2531
Fragments arranged by size:
2835 2531 1038 920 190 148 124 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2690 2722 4490
Size: 32 135 32 599 32 487 32 1768
Cuts at: 4490 4522 4781 4813 4955 4987 6442 6474 6516
Size: 32 259 32 142 32 1455 32 42
Cuts at: 6516 6548 1373
Size: 32 2626
Fragments arranged by size:
2626 1768 1455 599 487 259 142 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3820 5145 5391 669
Size: 988 281 1882 1325 246 3079
Fragments arranged by size:
3079 1882 1325 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7801
HpaI GTT'AAC
Cuts at: 5391 5391
Size: 7801
KasI G'GCGC_C
Cuts at: 2594 2947 3071 2594
Size: 353 124 7324
Fragments arranged by size:
7324 353 124
KpnI G_GTAC'C
Cuts at: 5126 5126
Size: 7801
MfeI C'AATT_G
Cuts at: 5400 5400
Size: 7801
MluI A'CGCG_T
Cuts at: 7677 7677
Size: 7801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4416 4476 4563 4605 5070 6915 7099 1305
Size: 3111 60 87 42 465 1845 184 2007
Fragments arranged by size:
3111 2007 1845 465 184 87 60 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2483 3869 4703 10
Size: 54 1425 343 651 1386 834 3108
Fragments arranged by size:
3108 1425 1386 834 651 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2642 2682 4241 4981 5759 6118 6277
Size: 648 1476 40 1559 740 778 359 159
Cuts at: 6277 518
Size: 2042
Fragments arranged by size:
2042 1559 1476 778 740 648 359 159
40
MspA1I CmG'CkG
Cuts at: 1606 2403 3341 3930 3991 4105 4180 4327 5217
Size: 797 938 589 61 114 75 147 890
Cuts at: 5217 5894 7042 7287 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 890 797 677 589 245
147 114 75 61
NaeI GCC'GGC
Cuts at: 2086 3306 3648 4532 2086
Size: 1220 342 884 5355
Fragments arranged by size:
5355 1220 884 342
NarI GG'CG_CC
Cuts at: 2595 2948 3072 2595
Size: 353 124 7324
Fragments arranged by size:
7324 353 124
NcoI C'CATG_G
Cuts at: 513 1065 3783 513
Size: 552 2718 4531
Fragments arranged by size:
4531 2718 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7801
NgoMIV G'CCGG_C
Cuts at: 2084 3304 3646 4530 2084
Size: 1220 342 884 5355
Fragments arranged by size:
5355 1220 884 342
NheI G'CTAG_C
Cuts at: 1052 2122 1052
Size: 1070 6731
NotI GC'GGCC_GC
Cuts at: 5248 5248
Size: 7801
NspI r_CATG'y
Cuts at: 1132 1229 2800 4213 5159 6704 1132
Size: 97 1571 1413 946 1545 2229
Fragments arranged by size:
2229 1571 1545 1413 946 97
PasI CC'CwG_GG
Cuts at: 1348 3740 1348
Size: 2392 5409
PciI A'CATG_T
Cuts at: 6700 6700
Size: 7801
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2724 3175 4699 1108
Size: 1616 451 1524 4210
Fragments arranged by size:
4210 1616 1524 451
PmeI GTTT'AAAC
Cuts at: 5105 5105
Size: 7801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5870 5902 7383 7415 5870
Size: 32 1481 32 6256
Fragments arranged by size:
6256 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2830 2941 3487 2830
Size: 111 546 7144
Fragments arranged by size:
7144 546 111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7801
PsiI TTA'TAA
Cuts at: 5371 5371
Size: 7801
PspOMI G'GGCC_C
Cuts at: 3949 4633 4939 3949
Size: 684 306 6811
Fragments arranged by size:
6811 684 306
PspXI vC'TCGA_Gb
Cuts at: 4585 4585
Size: 7801
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4801 4833 4801
Size: 32 7769
PstI C_TGCA'G
Cuts at: 830 2408 2496 2624 3156 4572 5153 830
Size: 1578 88 128 532 1416 581 3478
Fragments arranged by size:
3478 1578 1416 581 532 128 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7801
PvuII CAG'CTG
Cuts at: 2403 2403
Size: 7801
SacI G_AGCT'C
Cuts at: 2330 3863 2330
Size: 1533 6268
SacII CC_GC'GG
Cuts at: 3342 3342
Size: 7801
SalI G'TCGA_C
Cuts at: 1936 5143 1936
Size: 3207 4594
SapI GCTCTTCn'nnn_
Cuts at: 2146 3851 2146
Size: 1705 6096
SbfI CC_TGCA'GG
Cuts at: 4572 5153 4572
Size: 581 7220
ScaI AGT'ACT
Cuts at: 6049 7761 6049
Size: 1712 6089
SfcI C'TryA_G
Cuts at: 826 1045 2404 2492 2620 3152 4568 5149 6284
Size: 219 1359 88 128 532 1416 581 1135
Cuts at: 6284 6965 7156 826
Size: 681 191 1471
Fragments arranged by size:
1471 1416 1359 1135 681 581 532 219
191 128 88
SfoI GGC'GCC
Cuts at: 2596 2949 3073 2596
Size: 353 124 7324
Fragments arranged by size:
7324 353 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7801
SmaI CCC'GGG
Cuts at: 1872 3627 5128 1872
Size: 1755 1501 4545
Fragments arranged by size:
4545 1755 1501
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2148 3116 3524 4585 5905 6806
Size: 197 926 205 968 408 1061 1320 901
Cuts at: 6806 7068 820
Size: 262 1553
Fragments arranged by size:
1553 1320 1061 968 926 901 408 262
205 197
SnaBI TAC'GTA
Cuts at: 493 5592 493
Size: 5099 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7801
SphI G_CATG'C
Cuts at: 1132 4213 5159 1132
Size: 3081 946 3774
Fragments arranged by size:
3774 3081 946
SspI AAT'ATT
Cuts at: 5 52 5725 5
Size: 47 5673 2081
Fragments arranged by size:
5673 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7801
StyI C'CwwG_G
Cuts at: 513 1065 3009 3504 3783 3870 5240 513
Size: 552 1944 495 279 87 1370 3074
Fragments arranged by size:
3074 1944 1370 552 495 279 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3305 4148 5828 5845 5998 6183 6628
Size: 1255 1725 843 1680 17 153 185 445
Cuts at: 6628 325
Size: 1498
Fragments arranged by size:
1725 1680 1498 1255 843 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 4191 4614
Size: 274 80 33 51 1443 822 1392 423
Cuts at: 4614 6047 7759 96
Size: 1433 1712 138
Fragments arranged by size:
1712 1443 1433 1392 822 423 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2156 2188 4753 4785 7761 7793 2156
Size: 32 2565 32 2976 32 2164
Fragments arranged by size:
2976 2565 2164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2183 1316
Size: 867 6934
XbaI T'CTAG_A
Cuts at: 5137 5137
Size: 7801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2905 3511 3790 4933 2266
Size: 639 606 279 1143 5134
Fragments arranged by size:
5134 1143 639 606 279
XhoI C'TCGA_G
Cuts at: 1943 2148 4585 1943
Size: 205 2437 5159
Fragments arranged by size:
5159 2437 205
XmaI C'CCGG_G
Cuts at: 1870 3625 5126 1870
Size: 1755 1501 4545
Fragments arranged by size:
4545 1755 1501
XmnI GAAnn'nnTTC
Cuts at: 4813 5930 4813
Size: 1117 6684
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2502 5609 276
Size: 53 83 186 936 968 3107 2468
Fragments arranged by size:
3107 2468 968 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
RsrII SanDI SexAI SfiI SrfI SwaI