Restriction map for
pFN21AB9644
(Circular) (Six-base) MAPSORT of: pFN21AB9644.seq Check: 9753 from: 1 to: 5242
pFN21AB9644 5242 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3052 278
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2585 1937
Size: 648 4594
Acc65I G'GTAC_C
Cuts at: 2563 2563
Size: 5242
AclI AA'CG_TT
Cuts at: 1563 3369 3742 1563
Size: 1806 373 3063
Fragments arranged by size:
3063 1806 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 2292 3305 4689 4957 1632
Size: 567 93 1013 1384 268 1917
Fragments arranged by size:
1917 1384 1013 567 268 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5045
AflIII A'CryG_T
Cuts at: 1203 4141 5118 1203
Size: 2938 977 1327
Fragments arranged by size:
2938 1327 977
AgeI A'CCGG_T
Cuts at: 4073 4073
Size: 5242
AhdI GACnn_n'nnGTC
Cuts at: 3971 3971
Size: 5242
AleI CACnn'nnGTG
Cuts at: 1166 2525 1166
Size: 1359 3883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2543 1184
Size: 1359 3883
AlwNI CAG_nnn'CTG
Cuts at: 2096 2413 2985 4557 2096
Size: 317 572 1572 2781
Fragments arranged by size:
2781 1572 572 317
ApaI G_GGCC'C
Cuts at: 2238 2503 2238
Size: 265 4977
ApaLI G'TGCA_C
Cuts at: 3299 4455 3299
Size: 1156 4086
ApoI r'AATT_y
Cuts at: 1493 2551 2777 2929 5038 1493
Size: 1058 226 152 2109 1697
Fragments arranged by size:
2109 1697 1058 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3796 160
Size: 872 2764 1606
Fragments arranged by size:
2764 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2231 2315 2370 2486 2496 2567
Size: 73 253 35 84 55 116 10 71
Cuts at: 2567 1870
Size: 4545
Fragments arranged by size:
4545 253 116 84 73 71 55 35
10
BamHI G'GATC_C
Cuts at: 2508 2572 2939 2508
Size: 64 367 4811
Fragments arranged by size:
4811 367 64
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2563 4018 618
Size: 325 217 627 266 510 1455 1842
Fragments arranged by size:
1842 1455 627 510 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2090 2117 2238 2503 2561 1391
Size: 699 27 121 265 58 4072
Fragments arranged by size:
4072 699 265 121 58 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3433 3467 1903
Size: 34 1496 34 3678
Fragments arranged by size:
3678 1496 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3134 4344 3134
Size: 1210 4032
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5242
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2213 2233
Size: 107 122 71 631 605 337 204 20
Cuts at: 2233 2298 3853 136
Size: 65 1555 1525
Fragments arranged by size:
1555 1525 631 605 337 204 122 107
71 65 20
BglII A'GATC_T
Cuts at: 5237 5237
Size: 5242
BlpI GC'TnA_GC
Cuts at: 2089 2659 2089
Size: 570 4672
Bme1580I G_kGCm'C
Cuts at: 1790 2238 2503 3303 4459 1790
Size: 448 265 800 1156 2573
Fragments arranged by size:
2573 1156 800 448 265
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5242
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 2973 3931
Size: 416 53 83 399 24 629 927 958
Cuts at: 3931 442
Size: 1753
Fragments arranged by size:
1753 958 927 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 3902 1239
Size: 1001 1662 2579
Fragments arranged by size:
2579 1662 1001
Bpu10I CC'TnA_GC
Cuts at: 2097 2148 2097
Size: 51 5191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3367 4232 4530 3367
Size: 865 298 4079
Fragments arranged by size:
4079 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3905 882
Size: 617 2406 2219
Fragments arranged by size:
2406 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3033 493
Size: 711 1829 2702
Fragments arranged by size:
2702 1829 711
BsaBI GATnn'nnATC
Cuts at: 5236 5236
Size: 5242
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3049 3431 4066 275
Size: 53 83 186 936 1516 382 635 1451
Fragments arranged by size:
1516 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2059 3675 4073 4347 4494 4905 2059
Size: 1616 398 274 147 411 2396
Fragments arranged by size:
2396 1616 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5017
Fragments arranged by size:
5017 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2107 1186
Size: 921 4321
BseYI C'CCAG_C
Cuts at: 4445 4941 4445
Size: 496 4746
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4669
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2692 3453 4481 1540
Size: 275 186 691 761 1028 2301
Fragments arranged by size:
2301 1028 761 691 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 3303 3388 4459 2090
Size: 1213 85 1071 2873
Fragments arranged by size:
2873 1213 1071 85
BsmI GAATG_Cn'
Cuts at: 2753 2846 2753
Size: 93 5149
BsmBI CGTCTCn'nnnn_
Cuts at: 2312 2312
Size: 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2117 2238 2503 2561 3303 3388
Size: 399 300 27 121 265 58 742 85
Cuts at: 3388 4459 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 300 265 121 85
58 27
BspEI T'CCGG_A
Cuts at: 4905 4905
Size: 5242
BspHI T'CATG_A
Cuts at: 3131 4861 3131
Size: 1730 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2597 844
Size: 1753 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3129 1394
Size: 429 1306 3507
Fragments arranged by size:
3507 1306 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3737 3911 65
Size: 1183 2489 174 1396
Fragments arranged by size:
2489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2297 3886 4073 1700
Size: 110 268 219 1589 187 2869
Fragments arranged by size:
2869 1589 268 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5242
BssHII G'CGCG_C
Cuts at: 1925 2276 1925
Size: 351 4891
BssSI C'ACGA_G
Cuts at: 3302 4314 3302
Size: 1012 4230
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5242
BstBI TT'CG_AA
Cuts at: 4046 4046
Size: 5242
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5242
BstYI r'GATC_y
Cuts at: 1972 2508 2572 2939 3324 3341 4793 4879 4891
Size: 536 64 367 385 17 1452 86 12
Cuts at: 4891 5237 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 536 385 367 346 86 64
17 12
Bsu36I CC'TnA_GG
Cuts at: 2259 2259
Size: 5242
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2169 2211 513
Size: 552 939 42 123 42 3544
Fragments arranged by size:
3544 939 552 123 42 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4503
Fragments arranged by size:
4503 683 56
BtsI GCAGTG_nn'
Cuts at: 2758 3552 3572 2758
Size: 794 20 4428
Fragments arranged by size:
4428 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5207
DraI TTT'AAA
Cuts at: 2546 2546
Size: 5242
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4249 809
Size: 507 2933 1802
Fragments arranged by size:
2933 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2689 3578
Size: 54 1425 50 275 18 201 658 889
Cuts at: 3578 5207 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 658 275 201 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2689 1537
Size: 275 877 4090
Fragments arranged by size:
4090 877 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3172 1353
Size: 1819 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2315 2501 2521 3829 4203 4349
Size: 1064 138 726 186 20 1308 374 146
Cuts at: 4349 387
Size: 1280
Fragments arranged by size:
1308 1280 1064 726 374 186 146 138
20
EcoICRI GAG'CTC
Cuts at: 2088 2088
Size: 5242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2240 2292 3305 3902 4689 4957
Size: 393 567 41 52 1013 597 787 268
Cuts at: 4957 1239
Size: 1524
Fragments arranged by size:
1524 1013 787 597 567 393 268 52
41
EcoNI CCTnn'n_nnAGG
Cuts at: 2486 2486
Size: 5242
EcoO109I rG'GnC_Cy
Cuts at: 2291 2366 2291
Size: 75 5167
EcoRI G'AATT_C
Cuts at: 2551 2929 2551
Size: 378 4864
FspI TGC'GCA
Cuts at: 2531 3748 2531
Size: 1217 4025
FspAI rTGC'GCAy
Cuts at: 2531 2531
Size: 5242
HaeII r_GCGC'y
Cuts at: 1678 2258 4389 1678
Size: 580 2131 2531
Fragments arranged by size:
2531 2131 580
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3883 3915 3957 3989 1373
Size: 32 135 32 2311 32 42 32 2626
Fragments arranged by size:
2626 2311 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2586 2832 669
Size: 988 281 648 246 3079
Fragments arranged by size:
3079 988 648 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5242
HpaI GTT'AAC
Cuts at: 2832 2832
Size: 5242
KpnI G_GTAC'C
Cuts at: 2567 2567
Size: 5242
MfeI C'AATT_G
Cuts at: 2841 2841
Size: 5242
MluI A'CGCG_T
Cuts at: 5118 5118
Size: 5242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4356 4540 1305
Size: 3051 184 2007
Fragments arranged by size:
3051 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3420
Fragments arranged by size:
3420 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 3200 3559 3718 518
Size: 648 1359 675 359 159 2042
Fragments arranged by size:
2042 1359 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2171 2213 2389 2470 2658 3335 4483
Size: 442 123 42 176 81 188 677 1148
Cuts at: 4483 4728 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 442 245 188 176 123
81 42
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 5242
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3751
Fragments arranged by size:
3751 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5242
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 5242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5242
NotI GC'GGCC_GC
Cuts at: 2689 2689
Size: 5242
NspI r_CATG'y
Cuts at: 1132 1229 2600 4145 1132
Size: 97 1371 1545 2229
Fragments arranged by size:
2229 1545 1371 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5242
PciI A'CATG_T
Cuts at: 4141 4141
Size: 5242
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5242
PmeI GTTT'AAAC
Cuts at: 2546 2546
Size: 5242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 4824 4856 3311
Size: 32 1481 32 3697
Fragments arranged by size:
3697 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2366 2366
Size: 5242
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5242
PsiI TTA'TAA
Cuts at: 2812 2812
Size: 5242
PspOMI G'GGCC_C
Cuts at: 2234 2499 2234
Size: 265 4977
PstI C_TGCA'G
Cuts at: 830 2594 830
Size: 1764 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5242
PvuII CAG'CTG
Cuts at: 2389 2470 2389
Size: 81 5161
SacI G_AGCT'C
Cuts at: 2090 2090
Size: 5242
SacII CC_GC'GG
Cuts at: 2049 2172 2214 2049
Size: 123 42 5077
Fragments arranged by size:
5077 123 42
SalI G'TCGA_C
Cuts at: 1936 2584 1936
Size: 648 4594
SanDI GG'GwC_CC
Cuts at: 2366 2366
Size: 5242
SbfI CC_TGCA'GG
Cuts at: 2594 2594
Size: 5242
ScaI AGT'ACT
Cuts at: 3490 5202 3490
Size: 1712 3530
SfcI C'TryA_G
Cuts at: 826 1045 2590 3725 4406 4597 826
Size: 219 1545 1135 681 191 1471
Fragments arranged by size:
1545 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2213 2213
Size: 5242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5242
SmaI CCC'GGG
Cuts at: 1872 2372 2569 1872
Size: 500 197 4545
Fragments arranged by size:
4545 500 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3346 4247 4509 820
Size: 197 926 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3033 493
Size: 2540 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5242
SphI G_CATG'C
Cuts at: 1132 2600 1132
Size: 1468 3774
SspI AAT'ATT
Cuts at: 5 52 3166 5
Size: 47 3114 2081
Fragments arranged by size:
3114 2081 47
StuI AGG'CCT
Cuts at: 1517 2132 1517
Size: 615 4627
StyI C'CwwG_G
Cuts at: 513 1065 2004 2433 2681 513
Size: 552 939 429 248 3074
Fragments arranged by size:
3074 939 552 429 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 3269 3286 3439 3624 4069 325
Size: 1255 720 969 17 153 185 445 1498
Fragments arranged by size:
1498 1255 969 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3488 5200 96
Size: 274 80 33 51 1443 1511 1712 138
Fragments arranged by size:
1712 1511 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2026 2058 5202 5234 2026
Size: 32 3144 32 2034
Fragments arranged by size:
3144 2034 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2310 1316
Size: 994 4248
XbaI T'CTAG_A
Cuts at: 2303 2578 2303
Size: 275 4967
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5242
XmaI C'CCGG_G
Cuts at: 1870 2370 2567 1870
Size: 500 197 4545
Fragments arranged by size:
4545 500 197
XmnI GAAnn'nnTTC
Cuts at: 3371 3371
Size: 5242
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3050 276
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI
FseI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SapI SexAI SfoI SrfI SwaI
XcmI