Restriction map for
pFN21AB9642
(Circular) (Six-base) MAPSORT of: pFN21AB9642.seq Check: 6450 from: 1 to: 6484
pFN21AB9642 6484 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3239 3239
Size: 6484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2996 3212 4547 5931 6199 1632
Size: 1364 216 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1364 1335 268 216
AfeI AGC'GCT
Cuts at: 2570 2570
Size: 6484
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 5383 6360 1203
Size: 4180 977 1327
Fragments arranged by size:
4180 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 3641 1166
Size: 2475 4009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6484
AlwNI CAG_nnn'CTG
Cuts at: 2485 2591 4227 5799 2485
Size: 106 1636 1572 3170
Fragments arranged by size:
3170 1636 1572 106
ApaI G_GGCC'C
Cuts at: 2674 2674
Size: 6484
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 3793 4019 4171 6280 1493
Size: 2300 226 152 2109 1697
Fragments arranged by size:
2300 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3809 1870
Size: 73 1866 4545
Fragments arranged by size:
4545 1866 73
BamHI G'GATC_C
Cuts at: 3564 3814 4181 3564
Size: 250 367 5867
Fragments arranged by size:
5867 367 250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 3724 3805 5260 618
Size: 325 217 627 1016 921 81 1455 1842
Fragments arranged by size:
1842 1455 1016 921 627 325 217 81
BanII G_rGCy'C
Cuts at: 1391 2196 2229 2674 2760 2801 3803 1391
Size: 805 33 445 86 41 1002 4072
Fragments arranged by size:
4072 1002 805 445 86 41 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2377 928
Size: 971 478 5035
Fragments arranged by size:
5035 971 478
BbvCI CC'TCA_GC
Cuts at: 2276 2579 2828 2276
Size: 303 249 5932
Fragments arranged by size:
5932 303 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2339 2373 4675 4709 1903
Size: 34 402 34 2302 34 3678
Fragments arranged by size:
3678 2302 402 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2044 3220 5095
Size: 107 122 71 631 605 372 1176 1875
Cuts at: 5095 136
Size: 1525
Fragments arranged by size:
1875 1525 1176 631 605 372 122 107
71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 2891 3901 2891
Size: 1010 5474
Bme1580I G_kGCm'C
Cuts at: 1790 2674 2808 3225 4545 5701 1790
Size: 884 134 417 1320 1156 2573
Fragments arranged by size:
2573 1320 1156 884 417 134
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4215 5173 442
Size: 416 53 83 399 24 2798 958 1753
Fragments arranged by size:
2798 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2543 1056
Size: 1487 4997
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2177 2190 2213 2246 2462 2507 2585
Size: 858 80 13 23 33 216 45 78
Cuts at: 2585 2723 2747 2894 3027 3201 3333 3431 5144
Size: 138 24 147 133 174 132 98 1713
Cuts at: 5144 1239
Size: 2579
Fragments arranged by size:
2579 1713 858 216 174 147 138 133
132 98 80 78 45 33 24 23
13
Bpu10I CC'TnA_GC
Cuts at: 2252 2276 2579 2828 2252
Size: 24 303 249 5908
Fragments arranged by size:
5908 303 249 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2678 3082 4609 5474 5772 2321
Size: 357 404 1527 865 298 3033
Fragments arranged by size:
3033 1527 865 404 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3315 5147 882
Size: 617 1816 1832 2219
Fragments arranged by size:
2219 1832 1816 617
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4291 4673 5308 275
Size: 53 83 186 936 2758 382 635 1451
Fragments arranged by size:
2758 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 1178
Size: 30 165 30 699 30 5530
Fragments arranged by size:
5530 699 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2057 3092 3296 1186
Size: 868 3 1035 204 4374
Fragments arranged by size:
4374 1035 868 204 3
BseYI C'CCAG_C
Cuts at: 2121 2666 2683 2878 3325 3367 3377 3473 5687
Size: 545 17 195 447 42 10 96 2214
Cuts at: 5687 6183 2121
Size: 496 2422
Fragments arranged by size:
2422 2214 545 496 447 195 96 42
17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2098 3446 1163
Size: 573 362 1348 4201
Fragments arranged by size:
4201 1348 573 362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3934 4695 5723 1540
Size: 275 186 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2196 2555 2801 4545 4630 5701 2139
Size: 57 359 246 1744 85 1071 2922
Fragments arranged by size:
2922 1744 1071 359 246 85 57
BsmI GAATG_Cn'
Cuts at: 2244 3995 4088 2244
Size: 1751 93 4640
Fragments arranged by size:
4640 1751 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2615 2615
Size: 6484
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2196 2229 2555 2674 2760 2801
Size: 399 349 57 33 326 119 86 41
Cuts at: 2801 2808 3225 3803 4545 4630 5701 1391
Size: 7 417 578 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 417 399 349 326
119 86 85 57 41 33 7
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3239 3839 844
Size: 2395 600 3489
Fragments arranged by size:
3489 2395 600
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 2085 3420 4371 1394
Size: 429 230 32 1335 951 3507
Fragments arranged by size:
3507 1335 951 429 230 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 2946 3273 3472 4979 5153 65
Size: 1183 1698 327 199 1507 174 1396
Fragments arranged by size:
1698 1507 1396 1183 327 199 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5128 5315 1700
Size: 110 3318 187 2869
Fragments arranged by size:
3318 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 794
Size: 1445 5039
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 3055 3758 1184
Size: 1871 703 3910
Fragments arranged by size:
3910 1871 703
BstXI CCAn_nnnn'nTGG
Cuts at: 3610 3610
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 2706 2848 3414 3564 3814 4181 4566 4583
Size: 734 142 566 150 250 367 385 17
Cuts at: 4583 6035 6121 6133 6479 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 734 566 385 367 346 250
150 142 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2342 2630 2837 3488 2342
Size: 288 207 651 5338
Fragments arranged by size:
5338 651 288 207
BtgI C'CryG_G
Cuts at: 513 1065 2004 2042 2317 2965 3603 513
Size: 552 939 38 275 648 638 3394
Fragments arranged by size:
3394 939 648 638 552 275 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 3768 4000 4794 4814 3768
Size: 232 794 20 5438
Fragments arranged by size:
5438 794 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3053 5491 809
Size: 507 1737 2438 1802
Fragments arranged by size:
2438 1802 1737 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2717 2775
Size: 54 1425 50 275 18 313 574 58
Cuts at: 2775 3600 3931 4820 6449 8
Size: 825 331 889 1629 43
Fragments arranged by size:
1629 1425 889 825 574 331 313 275
58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 3179 3785 4414 1353
Size: 1094 732 606 629 3423
Fragments arranged by size:
3423 1094 732 629 606
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2411 3110 5071 5445 5591 387
Size: 1064 138 822 699 1961 374 146 1280
Fragments arranged by size:
1961 1280 1064 822 699 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 2799 2194
Size: 605 5879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2177 2190 2213 2246 2462 2507
Size: 393 465 80 13 23 33 216 45
Cuts at: 2507 2585 2723 2747 2894 2996 3027 3201 3212
Size: 78 138 24 147 102 31 174 11
Cuts at: 3212 3333 3431 4547 5144 5931 6199 1239
Size: 121 98 1116 597 787 268 1524
Fragments arranged by size:
1524 1116 787 597 465 393 268 216
174 147 138 121 102 98 80 78
45 33 31 24 23 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 3346 2261
Size: 1085 5399
EcoO109I rG'GnC_Cy
Cuts at: 2310 2375 2526 2670 2671 2842 3260 3391 3444
Size: 65 151 144 1 171 418 131 53
Cuts at: 3444 3492 3572 2310
Size: 48 80 5222
Fragments arranged by size:
5222 418 171 151 144 131 80 65
53 48 1
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 2572 5631 1678
Size: 894 3059 2531
Fragments arranged by size:
3059 2531 894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 3337 3369 3375
Size: 32 135 32 530 32 1203 32 6
Cuts at: 3375 3407 3734 3766 5125 5157 5199 5231 1373
Size: 32 327 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 1203 530 327 135 42 32
32 32 32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5598 5782 1305
Size: 4293 184 2007
Fragments arranged by size:
4293 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 2719 2777 3602 10
Size: 54 1425 343 313 574 58 825 2892
Fragments arranged by size:
2892 1425 825 574 343 313 58 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3512 3554 3641 4442 4801 4960 518
Size: 648 2346 42 87 801 359 159 2042
Fragments arranged by size:
2346 2042 801 648 359 159 87 42
MspA1I CmG'CkG
Cuts at: 1606 2094 2666 3900 4577 5725 5970 1606
Size: 488 572 1234 677 1148 245 2120
Fragments arranged by size:
2120 1234 1148 677 572 488 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2042 2317 2965 3603 513
Size: 552 939 38 275 648 638 3394
Fragments arranged by size:
3394 939 648 638 552 275 38
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 2539 1052
Size: 1487 4997
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 2937 3145 3296 3842 5387 1132
Size: 97 1708 208 151 546 1545 2229
Fragments arranged by size:
2229 1708 1545 546 208 151 97
PasI CC'CwG_GG
Cuts at: 1348 2741 3569 1348
Size: 1393 828 4263
Fragments arranged by size:
4263 1393 828
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2269 2485 2989 1108
Size: 1161 216 504 4603
Fragments arranged by size:
4603 1161 504 216
PfoI T'CCnGG_A
Cuts at: 2196 2262 2844 2910 2988 3341 2196
Size: 66 582 66 78 353 5339
Fragments arranged by size:
5339 582 353 78 66 66
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2842 3391 3492 2842
Size: 549 101 5834
Fragments arranged by size:
5834 549 101
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2670 2670
Size: 6484
PstI C_TGCA'G
Cuts at: 830 2352 3836 830
Size: 1522 1484 3478
Fragments arranged by size:
3478 1522 1484
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2094 2666 2094
Size: 572 5912
SacI G_AGCT'C
Cuts at: 2196 2801 2196
Size: 605 5879
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SapI GCTCTTCn'nnn_
Cuts at: 3179 3785 3179
Size: 606 5878
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SexAI A'CCwGG_T
Cuts at: 2505 2505
Size: 6484
SfcI C'TryA_G
Cuts at: 826 1045 2348 3832 4967 5648 5839 826
Size: 219 1303 1484 1135 681 191 1471
Fragments arranged by size:
1484 1471 1303 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2044 2044
Size: 6484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 2693 3061 4588 5489 5751
Size: 197 926 357 393 368 1527 901 262
Cuts at: 5751 820
Size: 1553
Fragments arranged by size:
1553 1527 926 901 393 368 357 262
197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 2620 152
Size: 2468 4016
SphI G_CATG'C
Cuts at: 1132 2937 3296 3842 1132
Size: 1805 359 546 3774
Fragments arranged by size:
3774 1805 546 359
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 2394 3352 1517
Size: 877 958 4649
Fragments arranged by size:
4649 958 877
StyI C'CwwG_G
Cuts at: 513 1065 2004 2042 2317 2965 3603 3923 513
Size: 552 939 38 275 648 638 320 3074
Fragments arranged by size:
3074 939 648 638 552 320 275 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4511 4528 4681 4866 5311 325
Size: 1255 2931 17 153 185 445 1498
Fragments arranged by size:
2931 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4730 6442 96
Size: 274 80 33 51 1443 2753 1712 138
Fragments arranged by size:
2753 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3399 3431 6444 6476 3399
Size: 32 3013 32 3407
Fragments arranged by size:
3407 3013 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2031 2963 3134 2031
Size: 932 171 5381
Fragments arranged by size:
5381 932 171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SfoI SrfI SwaI