Restriction map for
pFN21AB9641
(Circular) (Six-base) MAPSORT of: pFN21AB9641.seq Check: 4662 from: 1 to: 5671
pFN21AB9641 5671 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 3734 5118 5386 1632
Size: 650 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 650 268
AfeI AGC'GCT
Cuts at: 2219 2693 2219
Size: 474 5197
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2055 1184
Size: 871 4800
AlwNI CAG_nnn'CTG
Cuts at: 2185 3414 4986 2185
Size: 1229 1572 2870
Fragments arranged by size:
2870 1572 1229
ApaI G_GGCC'C
Cuts at: 2155 2381 2155
Size: 226 5445
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2660 2996 1870
Size: 73 137 580 336 4545
Fragments arranged by size:
4545 580 336 137 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2666 2992 4447 618
Size: 325 217 627 283 596 326 1455 1842
Fragments arranged by size:
1842 1455 627 596 326 325 283 217
BanII G_rGCy'C
Cuts at: 1391 2087 2155 2381 2549 2589 2714 2990 1391
Size: 696 68 226 168 40 125 276 4072
Fragments arranged by size:
4072 696 276 226 168 125 68 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2155 2381 2669 2784 3732 4888 1790
Size: 283 82 226 288 115 948 1156 2573
Fragments arranged by size:
2573 1156 948 288 283 226 115 82
BmgBI CAC'GTC
Cuts at: 1318 2233 1318
Size: 915 4756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2686 2206
Size: 480 5191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4331 1239
Size: 3092 2579
Bpu10I CC'TnA_GC
Cuts at: 2086 2086
Size: 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 3796 4661 4959 2465
Size: 1331 865 298 3177
Fragments arranged by size:
3177 1331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 4334 882
Size: 617 1413 1422 2219
Fragments arranged by size:
2219 1422 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 2657 2846 2930 1186
Size: 1468 3 189 84 3927
Fragments arranged by size:
3927 1468 189 84 3
BseYI C'CCAG_C
Cuts at: 2047 2374 2381 4874 5370 2047
Size: 327 7 2493 496 2348
Fragments arranged by size:
2493 2348 496 327 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2500 2652 3121 3882 4910 1540
Size: 275 186 499 152 469 761 1028 2301
Fragments arranged by size:
2301 1028 761 499 469 275 186 152
BsiHKAI G_wGCw'C
Cuts at: 2364 3732 3817 4888 2364
Size: 1368 85 1071 3147
Fragments arranged by size:
3147 1368 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
BsmBI CGTCTCn'nnnn_
Cuts at: 2168 2168
Size: 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2087 2155 2364 2381 2549 2589
Size: 399 283 14 68 209 17 168 40
Cuts at: 2589 2669 2714 2784 2990 3732 3817 4888 1391
Size: 80 45 70 206 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 209 206 168
85 80 70 68 45 40 17 14
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3026 844
Size: 2182 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 4166 4340 65
Size: 1183 1317 1601 174 1396
Fragments arranged by size:
1601 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2647 4315 4502 1700
Size: 110 837 1668 187 2869
Fragments arranged by size:
2869 1668 837 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 2171 3731 4743 2171
Size: 1560 1012 3099
Fragments arranged by size:
3099 1560 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2320 2200
Size: 120 5551
BstYI r'GATC_y
Cuts at: 1972 2476 3001 3368 3753 3770 5222 5308 5320
Size: 504 525 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 525 504 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2325 513
Size: 552 939 321 3859
Fragments arranged by size:
3859 939 552 321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 3187 3981 4001 2137
Size: 1050 794 20 3807
Fragments arranged by size:
3807 1050 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2497 2610
Size: 54 1425 50 275 18 492 175 113
Cuts at: 2610 2649 3118 4007 5636 8
Size: 39 469 889 1629 43
Fragments arranged by size:
1629 1425 889 492 469 275 175 113
54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2497 2649 3118 1537
Size: 275 685 152 469 4090
Fragments arranged by size:
4090 685 469 275 152
EarI CTCTTCn'nnn_
Cuts at: 1353 3601 1353
Size: 2248 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2504 4258 4632 4778 387
Size: 1064 138 435 480 1754 374 146 1280
Fragments arranged by size:
1754 1280 1064 480 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 3734 4331 5118 5386 1239
Size: 393 650 1452 597 787 268 1524
Fragments arranged by size:
1524 1452 787 650 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2402
Size: 5671
EcoO109I rG'GnC_Cy
Cuts at: 2151 2152 2378 2534 2151
Size: 1 226 156 5288
Fragments arranged by size:
5288 226 156 1
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2713 2272
Size: 441 5230
FspI TGC'GCA
Cuts at: 2510 4177 2510
Size: 1667 4004
HaeII r_GCGC'y
Cuts at: 1678 2048 2221 2432 2695 4818 1678
Size: 370 173 211 263 2123 2531
Fragments arranged by size:
2531 2123 370 263 211 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2696 2728 4312 4344 4386
Size: 32 135 32 1124 32 1584 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 1584 1124 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 3015 3261 669
Size: 988 281 589 488 246 3079
Fragments arranged by size:
3079 988 589 488 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2739 4785 4969 1305
Size: 1434 2046 184 2007
Fragments arranged by size:
2046 2007 1434 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 10
Size: 54 1425 343 780 3069
Fragments arranged by size:
3069 1425 780 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2777 3629 3988 4147 518
Size: 648 1152 459 852 359 159 2042
Fragments arranged by size:
2042 1152 852 648 459 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2374 2927 3087 3764 4912 5157 1606
Size: 416 352 553 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 553 416 352 245 160
NaeI GCC'GGC
Cuts at: 2649 2649
Size: 5671
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4180
Fragments arranged by size:
4180 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NgoMIV G'CCGG_C
Cuts at: 2647 2647
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 2497 3118 2497
Size: 621 5050
NspI r_CATG'y
Cuts at: 1132 1229 3029 4574 1132
Size: 97 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2065 2156 1348
Size: 717 91 4863
Fragments arranged by size:
4863 717 91
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PspOMI G'GGCC_C
Cuts at: 2151 2377 2151
Size: 226 5445
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2450 2482 2450
Size: 32 5639
PstI C_TGCA'G
Cuts at: 830 2169 2946 3023 830
Size: 1339 777 77 3478
Fragments arranged by size:
3478 1339 777 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2374 2374
Size: 5671
RsrII CG'GwC_CG
Cuts at: 2011 2011
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 2468 2825 3919 5631 2468
Size: 357 1094 1712 2508
Fragments arranged by size:
2508 1712 1094 357
SfcI C'TryA_G
Cuts at: 826 1045 2165 2942 3019 4154 4835 5026 826
Size: 219 1120 777 77 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 777 681 219 191 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 3775 4676 4938 820
Size: 197 926 537 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 537 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 3110 513
Size: 552 939 243 863 3074
Fragments arranged by size:
3074 939 863 552 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2028 3698 3715 3868 4053 4498 325
Size: 1255 448 1670 17 153 185 445 1498
Fragments arranged by size:
1670 1498 1255 448 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 2823 3917
Size: 274 80 33 51 1443 489 357 1094
Cuts at: 3917 5629 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1094 489 357 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspXI SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI