Restriction map for
pFN21AB9638
(Circular) (Six-base) MAPSORT of: pFN21AB9638.seq Check: 198 from: 1 to: 6058
pFN21AB9638 6058 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2700 2700
Size: 6058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3237 3401 1937
Size: 1300 164 4594
Fragments arranged by size:
4594 1300 164
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4121 5505 5773 1632
Size: 2489 1384 268 1917
Fragments arranged by size:
2489 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 2862 2919 4957 5934 1203
Size: 1659 57 2038 977 1327
Fragments arranged by size:
2038 1659 1327 977 57
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AlwNI CAG_nnn'CTG
Cuts at: 2026 2158 3801 5373 2026
Size: 132 1643 1572 2711
Fragments arranged by size:
2711 1643 1572 132
ApaI G_GGCC'C
Cuts at: 2039 2420 2039
Size: 381 5677
ApaLI G'TGCA_C
Cuts at: 2354 4115 5271 2354
Size: 1761 1156 3141
Fragments arranged by size:
3141 1761 1156
ApoI r'AATT_y
Cuts at: 1493 3367 3593 3745 5854 1493
Size: 1874 226 152 2109 1697
Fragments arranged by size:
2109 1874 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2438 2471 2497 2698 3065 3383 1870
Size: 73 495 33 26 201 367 318 4545
Fragments arranged by size:
4545 495 367 318 201 73 33 26
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2300 2630 3379 4834
Size: 325 217 627 270 243 330 749 1455
Cuts at: 4834 618
Size: 1842
Fragments arranged by size:
1842 1455 749 627 330 325 270 243
217
BanII G_rGCy'C
Cuts at: 1391 2039 2420 3377 1391
Size: 648 381 957 4072
Fragments arranged by size:
4072 957 648 381
BbeI G_GCGC'C
Cuts at: 2634 2634
Size: 6058
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5087
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3022 3056 4249 4283 1903
Size: 34 1085 34 1193 34 3678
Fragments arranged by size:
3678 1193 1085 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 2090 2473
Size: 107 122 71 631 605 371 47 383
Cuts at: 2473 4669 136
Size: 2196 1525
Fragments arranged by size:
2196 1525 631 605 383 371 122 107
71 47
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 2906 3475 2906
Size: 569 5489
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2060 2358 2420 2849 3089 4119 5275
Size: 249 21 298 62 429 240 1030 1156
Cuts at: 5275 1790
Size: 2573
Fragments arranged by size:
2573 1156 1030 429 298 249 240 62
21
BmgBI CAC'GTC
Cuts at: 1318 2830 3036 1318
Size: 1512 206 4340
Fragments arranged by size:
4340 1512 206
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 3789 4747
Size: 416 53 83 399 24 795 1577 958
Cuts at: 4747 442
Size: 1753
Fragments arranged by size:
1753 1577 958 795 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 2789 2975 3158 4718 1239
Size: 1385 165 186 183 1560 2579
Fragments arranged by size:
2579 1560 1385 186 183 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3065 4183 5048 5346 2915
Size: 150 1118 865 298 3627
Fragments arranged by size:
3627 1118 865 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2954 4721 882
Size: 617 1455 1767 2219
Fragments arranged by size:
2219 1767 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2631 3865 4247
Size: 53 83 186 936 549 549 1234 382
Cuts at: 4247 4882 275
Size: 635 1451
Fragments arranged by size:
1451 1234 936 635 549 549 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5833
Fragments arranged by size:
5833 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2250 2972 1186
Size: 1064 722 4272
Fragments arranged by size:
4272 1064 722
BseYI C'CCAG_C
Cuts at: 2176 2463 2753 2809 2842 5261 5757 2176
Size: 287 290 56 33 2419 496 2477
Fragments arranged by size:
2477 2419 496 290 287 56 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 2549 2561 2657 2678 3302 3374
Size: 573 435 378 12 96 21 624 72
Cuts at: 3374 1163
Size: 3847
Fragments arranged by size:
3847 624 573 435 378 96 72 21
12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2308 3207 3508 4269 5297
Size: 275 186 111 196 899 301 761 1028
Cuts at: 5297 1540
Size: 2301
Fragments arranged by size:
2301 1028 899 761 301 275 196 186
111
BsiHKAI G_wGCw'C
Cuts at: 2358 2736 4119 4204 5275 2358
Size: 378 1383 85 1071 3141
Fragments arranged by size:
3141 1383 1071 378 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2060 2358 2420 2736 2849 3089
Size: 399 249 21 298 62 316 113 240
Cuts at: 3089 3377 4119 4204 5275 1391
Size: 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 316 298 288 249
240 113 85 62 21
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 2700 3346 3413 844
Size: 1190 666 646 67 3489
Fragments arranged by size:
3489 1190 666 646 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 3945 1394
Size: 429 647 1475 3507
Fragments arranged by size:
3507 1475 647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4553 4727 65
Size: 1183 3305 174 1396
Fragments arranged by size:
3305 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2258 2303 3113 3171 3206 3307 4702
Size: 110 448 45 810 58 35 101 1395
Cuts at: 4702 4889 1700
Size: 187 2869
Fragments arranged by size:
2869 1395 810 448 187 110 101 58
45 35
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 2345 3060 1925
Size: 420 715 4923
Fragments arranged by size:
4923 715 420
BssSI C'ACGA_G
Cuts at: 2168 2579 2591 3104 4118 5130 2168
Size: 411 12 513 1014 1012 3096
Fragments arranged by size:
3096 1014 1012 513 411 12
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2026 794
Size: 1232 4826
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2992 3046 2992
Size: 54 6004
BstYI r'GATC_y
Cuts at: 1972 3388 3755 4140 4157 5609 5695 5707 6053
Size: 1416 367 385 17 1452 86 12 346
Cuts at: 6053 1972
Size: 1977
Fragments arranged by size:
1977 1452 1416 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3238 3238
Size: 6058
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 2085 2112 2199 2229 2635
Size: 552 939 26 55 27 87 30 406
Cuts at: 2635 3225 513
Size: 590 3346
Fragments arranged by size:
3346 939 590 552 406 87 55 30
27 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3261 487
Size: 683 56 2035 3284
Fragments arranged by size:
3284 2035 683 56
BtsI GCAGTG_nn'
Cuts at: 3338 3574 4368 4388 3338
Size: 236 794 20 5008
Fragments arranged by size:
5008 794 236 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DraIII CAC_nnn'GTG
Cuts at: 2200 2200
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2968 5065 809
Size: 507 1652 2097 1802
Fragments arranged by size:
2097 1802 1652 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2109 2305
Size: 54 1425 50 275 18 177 102 196
Cuts at: 2305 2421 2520 2587 3204 3505 4394 6023 8
Size: 116 99 67 617 301 889 1629 43
Fragments arranged by size:
1629 1425 889 617 301 275 196 177
116 102 99 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2305 3204 3505 1537
Size: 275 297 196 899 301 4090
Fragments arranged by size:
4090 899 301 297 275 196
EarI CTCTTCn'nnn_
Cuts at: 1353 3988 1353
Size: 2635 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2061 2346 4645 5019 5165 387
Size: 1064 138 472 285 2299 374 146 1280
Fragments arranged by size:
2299 1280 1064 472 374 285 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2624 2789 2975 3158 4121 4718 5505
Size: 393 992 165 186 183 963 597 787
Cuts at: 5505 5773 1239
Size: 268 1524
Fragments arranged by size:
1524 992 963 787 597 393 268 186
183 165
EcoO109I rG'GnC_Cy
Cuts at: 2035 2036 2896 3257 2035
Size: 1 860 361 4836
Fragments arranged by size:
4836 860 361 1
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 6058
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 2634 2843 3317 5205 1678
Size: 956 209 474 1888 2531
Fragments arranged by size:
2531 1888 956 474 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373
Size: 32 135 32 3127 32 42 32 2626
Fragments arranged by size:
3127 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KasI G'GCGC_C
Cuts at: 2630 2630
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5172 5356 1305
Size: 3867 184 2007
Fragments arranged by size:
3867 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2589 10
Size: 54 1425 343 177 580 3479
Fragments arranged by size:
3479 1425 580 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 2652 3044 4016 4375 4534 518
Size: 648 1417 69 392 972 359 159 2042
Fragments arranged by size:
2042 1417 972 648 392 359 159 69
MspA1I CmG'CkG
Cuts at: 1606 2032 2087 2114 2448 2602 2797 2905 2938
Size: 426 55 27 334 154 195 108 33
Cuts at: 2938 2976 3136 3474 4151 5299 5544 1606
Size: 38 160 338 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 426 338 334 245 195
160 154 108 55 38 33 27
NaeI GCC'GGC
Cuts at: 2260 3208 3309 2260
Size: 948 101 5009
Fragments arranged by size:
5009 948 101
NarI GG'CG_CC
Cuts at: 2631 2631
Size: 6058
NcoI C'CATG_G
Cuts at: 513 1065 2004 3225 513
Size: 552 939 1221 3346
Fragments arranged by size:
3346 1221 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NgoMIV G'CCGG_C
Cuts at: 2258 3206 3307 2258
Size: 948 101 5009
Fragments arranged by size:
5009 948 101
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 2109 3505 2109
Size: 1396 4662
NspI r_CATG'y
Cuts at: 1132 1229 2129 2657 2923 3416 4961 1132
Size: 97 900 528 266 493 1545 2229
Fragments arranged by size:
2229 1545 900 528 493 266 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 2919 4957 2919
Size: 2038 4020
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2200 1108
Size: 1092 4966
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 2035 2416 2035
Size: 381 5677
PstI C_TGCA'G
Cuts at: 830 2904 3135 3410 830
Size: 2074 231 275 3478
Fragments arranged by size:
3478 2074 275 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2602 2905 3136 2602
Size: 303 231 5524
Fragments arranged by size:
5524 303 231
SacII CC_GC'GG
Cuts at: 2033 2088 2115 2033
Size: 55 27 5976
Fragments arranged by size:
5976 55 27
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2900 3131 3406 4541 5222 5413 826
Size: 219 1855 231 275 1135 681 191 1471
Fragments arranged by size:
1855 1471 1135 681 275 231 219 191
SfoI GGC'GCC
Cuts at: 2632 2632
Size: 6058
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 3307 1700
Size: 1607 4451
SmaI CCC'GGG
Cuts at: 1872 2440 2499 2700 3385 1872
Size: 568 59 201 685 4545
Fragments arranged by size:
4545 685 568 201 59
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2930 3065 3080 4162 5063 5325
Size: 197 926 987 135 15 1082 901 262
Cuts at: 5325 820
Size: 1553
Fragments arranged by size:
1553 1082 987 926 901 262 197 135
15
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 2129 3416 1132
Size: 997 1287 3774
Fragments arranged by size:
3774 1287 997
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2004 3225 3497 513
Size: 552 939 1221 272 3074
Fragments arranged by size:
3074 1221 939 552 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2978 4085 4102 4255 4440 4885 325
Size: 1255 1398 1107 17 153 185 445 1498
Fragments arranged by size:
1498 1398 1255 1107 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4304 6016 96
Size: 274 80 33 51 1443 2327 1712 138
Fragments arranged by size:
2327 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6018 6050 6018
Size: 32 6026
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 3065 1943
Size: 1122 4936
XmaI C'CCGG_G
Cuts at: 1870 2438 2497 2698 3383 1870
Size: 568 59 201 685 4545
Fragments arranged by size:
4545 685 568 201 59
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI
XcmI