Restriction map for
pFN21AB9635
(Circular) (Six-base) MAPSORT of: pFN21AB9635.seq Check: 4428 from: 1 to: 5674
pFN21AB9635 5674 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2258 3017 1937
Size: 321 759 4594
Fragments arranged by size:
4594 759 321
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3737 5121 5389 1632
Size: 2105 1384 268 1917
Fragments arranged by size:
2105 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2922 4573 5550 1203
Size: 1719 1651 977 1327
Fragments arranged by size:
1719 1651 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2381 1184
Size: 1197 4477
AlwNI CAG_nnn'CTG
Cuts at: 2329 2690 3417 4989 2329
Size: 361 727 1572 3014
Fragments arranged by size:
3014 1572 727 361
ApaLI G'TGCA_C
Cuts at: 2654 3731 4887 2654
Size: 1077 1156 3441
Fragments arranged by size:
3441 1156 1077
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 2999 1870
Size: 73 191 865 4545
Fragments arranged by size:
4545 865 191 73
BamHI G'GATC_C
Cuts at: 2738 3004 3371 2738
Size: 266 367 5041
Fragments arranged by size:
5041 367 266
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2902 2936 2995 4450
Size: 325 217 627 327 788 34 59 1455
Cuts at: 4450 618
Size: 1842
Fragments arranged by size:
1842 1455 788 627 327 325 217 59
34
BanII G_rGCy'C
Cuts at: 1391 2055 2186 2993 1391
Size: 664 131 807 4072
Fragments arranged by size:
4072 807 664 131
BbeI G_GCGC'C
Cuts at: 2940 2940
Size: 5674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2687 4285 136
Size: 107 122 71 631 605 1015 1598 1525
Fragments arranged by size:
1598 1525 1015 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2529 5669 2529
Size: 3140 2534
BlpI GC'TnA_GC
Cuts at: 2156 3091 2156
Size: 935 4739
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2658 3735 4891 1790
Size: 327 541 1077 1156 2573
Fragments arranged by size:
2573 1156 1077 541 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2118 2875 3405
Size: 416 53 83 399 24 701 757 530
Cuts at: 3405 4363 442
Size: 958 1753
Fragments arranged by size:
1753 958 757 701 530 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 4334 1239
Size: 1283 1812 2579
Fragments arranged by size:
2579 1812 1283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2771 3799 4664 4962 2321
Size: 450 1028 865 298 3033
Fragments arranged by size:
3033 1028 865 450 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2923 3465 493
Size: 711 1719 542 2702
Fragments arranged by size:
2702 1719 711 542
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2937 2967 3481
Size: 53 83 186 936 483 921 30 514
Cuts at: 3481 3863 4498 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 921 635 514 483 382 186
83 53 30
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 1186
Size: 1333 4341
BseYI C'CCAG_C
Cuts at: 2818 4877 5373 2818
Size: 2059 496 3119
Fragments arranged by size:
3119 2059 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2757 2811 3124 3885 4913 1540
Size: 275 186 756 54 313 761 1028 2301
Fragments arranged by size:
2301 1028 761 756 313 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2658 3735 3820 4891 2658
Size: 1077 85 1071 3441
Fragments arranged by size:
3441 1077 1071 85
BsiWI C'GTAC_G
Cuts at: 2412 2412
Size: 5674
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
BsmBI CGTCTCn'nnnn_
Cuts at: 2339 2339
Size: 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2117 2186 2658 2993 3735 3820
Size: 399 265 62 69 472 335 742 85
Cuts at: 3820 4891 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 335 265 85
69 62
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2850 3561 1394
Size: 429 1027 711 3507
Fragments arranged by size:
3507 1027 711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 2939 4318 4505 1700
Size: 110 261 868 1379 187 2869
Fragments arranged by size:
2869 1379 868 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 2193 2468 1925
Size: 268 275 5131
Fragments arranged by size:
5131 275 268
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 2690 2880 2895 794
Size: 1535 361 190 15 3573
Fragments arranged by size:
3573 1535 361 190 15
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 2549 2500
Size: 49 5625
BstYI r'GATC_y
Cuts at: 1972 2529 2738 3004 3371 3756 3773 5225 5311
Size: 557 209 266 367 385 17 1452 86
Cuts at: 5311 5323 5669 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 557 385 367 346 266 209
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2385 2649 2709 2805 513
Size: 552 939 381 264 60 96 3382
Fragments arranged by size:
3382 939 552 381 264 96 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2365 487
Size: 683 56 1139 3796
Fragments arranged by size:
3796 1139 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 2845 3190 3984 4004 2227
Size: 618 345 794 20 3897
Fragments arranged by size:
3897 794 618 345 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DraIII CAC_nnn'GTG
Cuts at: 2923 2923
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2808 3121 4010
Size: 54 1425 50 275 18 978 313 889
Cuts at: 4010 5639 8
Size: 1629 43
Fragments arranged by size:
1629 1425 978 889 313 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2808 3121 1537
Size: 275 996 313 4090
Fragments arranged by size:
4090 996 313 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 3737 4334 5121 5389 1239
Size: 393 890 1215 597 787 268 1524
Fragments arranged by size:
1524 1215 890 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2399
Size: 5674
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 2435 4180 2435
Size: 1745 3929
HaeII r_GCGC'y
Cuts at: 1678 2103 2163 2178 2727 2940 4821 1678
Size: 425 60 15 549 213 1881 2531
Fragments arranged by size:
2531 1881 549 425 213 60 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4315 4347 4389 4421 1373
Size: 32 135 32 2743 32 42 32 2626
Fragments arranged by size:
2743 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2438 748
Size: 1690 3984
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KasI G'GCGC_C
Cuts at: 2936 2936
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2988 4788 4972 1305
Size: 1683 1800 184 2007
Fragments arranged by size:
2007 1800 1683 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2483 3632 3991 4150 518
Size: 648 1218 99 1149 359 159 2042
Fragments arranged by size:
2042 1218 1149 648 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2094 2329 2651 2687 2702 2711 2807 2818
Size: 488 235 322 36 15 9 96 11
Cuts at: 2818 3090 3767 4915 5160 1606
Size: 272 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 488 322 272 245 235
96 36 15 11 9
NaeI GCC'GGC
Cuts at: 2073 2941 2073
Size: 868 4806
NarI GG'CG_CC
Cuts at: 2937 2937
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 2385 513
Size: 552 939 381 3802
Fragments arranged by size:
3802 939 552 381
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2071 2939 2071
Size: 868 4806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 2808 3121 2808
Size: 313 5361
NspI r_CATG'y
Cuts at: 1132 1229 2368 2878 2896 3032 4577 1132
Size: 97 1139 510 18 136 1545 2229
Fragments arranged by size:
2229 1545 1139 510 136 97 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PmlI CAC'GTG
Cuts at: 2923 2923
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2325 2707 2891 3026 830
Size: 1495 382 184 135 3478
Fragments arranged by size:
3478 1495 382 184 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2329 2329
Size: 5674
SacII CC_GC'GG
Cuts at: 2652 2712 2808 2652
Size: 60 96 5518
Fragments arranged by size:
5518 96 60
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2321 2703 2887 3022 4157 4838 5029
Size: 219 1276 382 184 135 1135 681 191
Cuts at: 5029 826
Size: 1471
Fragments arranged by size:
1471 1276 1135 681 382 219 191 184
135
SfoI GGC'GCC
Cuts at: 2938 2938
Size: 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 2786 3778 4679 4941 820
Size: 197 926 393 450 992 901 262 1553
Fragments arranged by size:
1553 992 926 901 450 393 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 2368 2878 3032 1132
Size: 1236 510 154 3774
Fragments arranged by size:
3774 1236 510 154
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 2385 2970 3113 513
Size: 552 939 381 585 143 3074
Fragments arranged by size:
3074 939 585 552 381 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2743 3701 3718 3871 4056 4501 325
Size: 1255 1163 958 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1163 958 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3920 5632 96
Size: 274 80 33 51 1443 1943 1712 138
Fragments arranged by size:
1943 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 2461
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI