Restriction map for
pFN21AB9619
(Circular) (Six-base) MAPSORT of: pFN21AB9619.seq Check: 2216 from: 1 to: 5746
pFN21AB9619 5746 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2953 2953
Size: 5746
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2520 3089 1937
Size: 583 569 4594
Fragments arranged by size:
4594 583 569
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3809 5193 5461 1632
Size: 2177 1384 268 1917
Fragments arranged by size:
2177 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 2472 4645 5622 1203
Size: 1269 2173 977 1327
Fragments arranged by size:
2173 1327 1269 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2846 1184
Size: 1662 4084
AlwNI CAG_nnn'CTG
Cuts at: 2579 3019 3489 5061 2579
Size: 440 470 1572 3264
Fragments arranged by size:
3264 1572 470 440
ApaI G_GGCC'C
Cuts at: 2074 2499 2511 2074
Size: 425 12 5309
Fragments arranged by size:
5309 425 12
ApaLI G'TGCA_C
Cuts at: 2008 3803 4959 2008
Size: 1795 1156 2795
Fragments arranged by size:
2795 1795 1156
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2320 2510 2587 3071 1870
Size: 73 377 190 77 484 4545
Fragments arranged by size:
4545 484 377 190 77 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 2708 3067 4522 618
Size: 325 217 627 807 114 359 1455 1842
Fragments arranged by size:
1842 1455 807 627 359 325 217 114
BanII G_rGCy'C
Cuts at: 1391 2074 2170 2225 2499 2511 2624 3065 1391
Size: 683 96 55 274 12 113 441 4072
Fragments arranged by size:
4072 683 441 274 113 96 55 12
BbeI G_GCGC'C
Cuts at: 2598 2712 2598
Size: 114 5632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2802 2912 928
Size: 971 903 110 3762
Fragments arranged by size:
3762 971 903 110
BbvCI CC'TCA_GC
Cuts at: 2558 2558
Size: 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2054 3638 4848 2054
Size: 1584 1210 2952
Fragments arranged by size:
2952 1584 1210
BclI T'GATC_A
Cuts at: 1548 2298 1548
Size: 750 4996
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2187 4357 136
Size: 107 122 71 631 605 515 2170 1525
Fragments arranged by size:
2170 1525 631 605 515 122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2074 2499 2511 3807 4963 1790
Size: 222 62 425 12 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 425 222 62 12
BmgBI CAC'GTC
Cuts at: 1318 2998 1318
Size: 1680 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2894 2912 4406 1239
Size: 1655 18 1494 2579
Fragments arranged by size:
2579 1655 1494 18
Bpu10I CC'TnA_GC
Cuts at: 2558 2558
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2897 3871 4736 5034 2228
Size: 669 974 865 298 2940
Fragments arranged by size:
2940 974 865 669 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2458 3537 493
Size: 711 1254 1079 2702
Fragments arranged by size:
2702 1254 1079 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2309 2385 2517 2595
Size: 53 83 186 936 776 76 132 78
Cuts at: 2595 2709 2763 3553 3935 4570 275
Size: 114 54 790 382 635 1451
Fragments arranged by size:
1451 936 790 776 635 382 186 132
114 83 78 76 54 53
BsaWI w'CCGG_w
Cuts at: 2145 4179 4577 4851 4998 5409 2145
Size: 2034 398 274 147 411 2482
Fragments arranged by size:
2482 2034 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 2942 1186
Size: 1222 534 3990
Fragments arranged by size:
3990 1222 534
BseYI C'CCAG_C
Cuts at: 2504 2572 2692 2970 4949 5445 2504
Size: 68 120 278 1979 496 2805
Fragments arranged by size:
2805 1979 496 278 120 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2385 2426 1163
Size: 573 649 41 4483
Fragments arranged by size:
4483 649 573 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 3196 3957 4985 1540
Size: 275 186 67 1128 761 1028 2301
Fragments arranged by size:
2301 1128 1028 761 275 186 67
BsiHKAI G_wGCw'C
Cuts at: 2012 2624 3807 3892 4963 2012
Size: 612 1183 85 1071 2795
Fragments arranged by size:
2795 1183 1071 612 85
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
BsmBI CGTCTCn'nnnn_
Cuts at: 2244 2244
Size: 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2074 2170 2225 2499 2511 2624
Size: 399 222 62 96 55 274 12 113
Cuts at: 2624 3065 3807 3892 4963 1391
Size: 441 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 274 222 113
96 85 62 55 12
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 2988 3635 5365 2988
Size: 647 1730 3369
Fragments arranged by size:
3369 1730 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2953 3101 844
Size: 2109 148 3489
Fragments arranged by size:
3489 2109 148
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4241 4415 65
Size: 1183 2993 174 1396
Fragments arranged by size:
2993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2231 2525 2672 2773 2838 2945 4390
Size: 110 421 294 147 101 65 107 1445
Cuts at: 4390 4577 1700
Size: 187 2869
Fragments arranged by size:
2869 1445 421 294 187 147 110 107
101 65
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 2750 2885 1925
Size: 825 135 4786
Fragments arranged by size:
4786 825 135
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2740 794
Size: 1946 3800
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 2791
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741
Size: 1104 367 385 17 1452 86 12 346
Cuts at: 5741 1972
Size: 1977
Fragments arranged by size:
1977 1452 1104 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2341 2704 513
Size: 552 1276 363 3555
Fragments arranged by size:
3555 1276 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2866 487
Size: 683 56 1640 3367
Fragments arranged by size:
3367 1640 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DraIII CAC_nnn'GTG
Cuts at: 2856 2856
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2420 4753 809
Size: 507 1104 2333 1802
Fragments arranged by size:
2333 1802 1104 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 2133 2677
Size: 54 1425 50 275 18 235 68 544
Cuts at: 2677 2847 3193 4082 5711 8
Size: 170 346 889 1629 43
Fragments arranged by size:
1629 1425 889 544 346 275 235 170
68 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2065 3193 1537
Size: 275 253 1128 4090
Fragments arranged by size:
4090 1128 275 253
EarI CTCTTCn'nnn_
Cuts at: 1353 2260 3676 1353
Size: 907 1416 3423
Fragments arranged by size:
3423 1416 907
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2622 2622
Size: 5746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2894 2912 3809 4406 5193 5461 1239
Size: 393 1262 18 897 597 787 268 1524
Fragments arranged by size:
1524 1262 897 787 597 393 268 18
EcoO109I rG'GnC_Cy
Cuts at: 2071 2495 2496 2071
Size: 424 1 5321
Fragments arranged by size:
5321 424 1
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2162 2487 2598 2712 4893 1678
Size: 484 325 111 114 2181 2531
Fragments arranged by size:
2531 2181 484 325 114 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4387 4419 4461 4493 1373
Size: 32 135 32 2815 32 42 32 2626
Fragments arranged by size:
2815 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2128 3090 3336 669
Size: 988 281 190 962 246 3079
Fragments arranged by size:
3079 988 962 281 246 190
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KasI G'GCGC_C
Cuts at: 2594 2708 2594
Size: 114 5632
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2679 10
Size: 54 1425 343 847 3077
Fragments arranged by size:
3077 1425 847 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2789 2813 3704 4063 4222 518
Size: 648 849 774 24 891 359 159 2042
Fragments arranged by size:
2042 891 849 774 648 359 159 24
MspA1I CmG'CkG
Cuts at: 1606 2343 2355 2692 2706 3016 3162 3839 4987
Size: 737 12 337 14 310 146 677 1148
Cuts at: 4987 5232 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 737 677 337 310 245 146
14 12
NaeI GCC'GGC
Cuts at: 2527 2947 2527
Size: 420 5326
NarI GG'CG_CC
Cuts at: 2595 2709 2595
Size: 114 5632
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NgoMIV G'CCGG_C
Cuts at: 2525 2945 2525
Size: 420 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2479 1348
Size: 1131 4615
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2955 2955
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PmlI CAC'GTG
Cuts at: 2458 2458
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2996 1613
Size: 1383 4363
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspOMI G'GGCC_C
Cuts at: 2070 2495 2507 2070
Size: 425 12 5309
Fragments arranged by size:
5309 425 12
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2692 3016 2692
Size: 324 5422
SacI G_AGCT'C
Cuts at: 2624 2624
Size: 5746
SacII CC_GC'GG
Cuts at: 2344 2707 2344
Size: 363 5383
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 2330 3994 5706 2330
Size: 1664 1712 2370
Fragments arranged by size:
2370 1712 1664
SfcI C'TryA_G
Cuts at: 826 1045 3094 4229 4910 5101 826
Size: 219 2049 1135 681 191 1471
Fragments arranged by size:
2049 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2596 2710 2596
Size: 114 5632
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 2231 2672 2773 1700
Size: 531 441 101 4673
Fragments arranged by size:
4673 531 441 101
SmaI CCC'GGG
Cuts at: 1872 2322 2512 3073 1872
Size: 450 190 561 4545
Fragments arranged by size:
4545 561 450 190
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2912 3850 4751 5013 820
Size: 197 926 300 669 938 901 262 1553
Fragments arranged by size:
1553 938 926 901 669 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 3185 513
Size: 552 2120 3074
Fragments arranged by size:
3074 2120 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3773 3790 3943 4128 4573 325
Size: 1255 2193 17 153 185 445 1498
Fragments arranged by size:
2193 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2328 3992 5704
Size: 274 80 33 51 1443 351 1664 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1712 1664 1443 351 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 2320 2510 3071 1870
Size: 450 190 561 4545
Fragments arranged by size:
4545 561 450 190
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PpuMI PspXI PsrI RsrII SanDI SapI
SexAI SfiI SrfI SwaI