Restriction map for
pFN21AB9616
(Circular) (Six-base) MAPSORT of: pFN21AB9616.seq Check: 573 from: 1 to: 5374
pFN21AB9616 5374 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 3117 4689 3117
Size: 1572 3802
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2168 2174 2226 2413 2699 1870
Size: 73 190 35 6 52 187 286 4545
Fragments arranged by size:
4545 286 190 187 73 52 35 6
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2510 2590 2695 4150
Size: 325 217 627 518 205 80 105 1455
Cuts at: 4150 618
Size: 1842
Fragments arranged by size:
1842 1455 627 518 325 217 205 105
80
BanII G_rGCy'C
Cuts at: 1391 2142 2175 2693 1391
Size: 751 33 518 4072
Fragments arranged by size:
4072 751 518 33
BbeI G_GCGC'C
Cuts at: 2309 2514 2594 2309
Size: 205 80 5089
Fragments arranged by size:
5089 205 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3565 3599 1903
Size: 34 1628 34 3678
Fragments arranged by size:
3678 1628 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2666 3985 136
Size: 107 122 71 631 605 994 1319 1525
Fragments arranged by size:
1525 1319 994 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2341 3435 4591 1790
Size: 551 1094 1156 2573
Fragments arranged by size:
2573 1156 1094 551
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 4063 442
Size: 416 53 83 399 24 1688 958 1753
Fragments arranged by size:
1753 1688 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4034 1239
Size: 2795 2579
Bpu10I CC'TnA_GC
Cuts at: 2384 2384
Size: 5374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 4037 882
Size: 617 816 1722 2219
Fragments arranged by size:
2219 1722 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2306 2511 2591 3181
Size: 53 83 186 936 773 205 80 590
Cuts at: 3181 3563 4198 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 773 635 590 382 205 186
83 80 53
BsaWI w'CCGG_w
Cuts at: 2097 2281 3807 4205 4479 4626 5037 2097
Size: 184 1526 398 274 147 411 2434
Fragments arranged by size:
2434 1526 411 398 274 184 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 1178
Size: 30 165 30 1041 30 4078
Fragments arranged by size:
4078 1041 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2465 1186
Size: 1147 132 4095
Fragments arranged by size:
4095 1147 132
BseYI C'CCAG_C
Cuts at: 4577 5073 4577
Size: 496 4878
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2078 2201 1163
Size: 573 297 45 123 4336
Fragments arranged by size:
4336 573 297 123 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 2247 2479 2824 3585 4613
Size: 275 186 192 54 232 345 761 1028
Cuts at: 4613 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 345 275 232 192 186
54
BsiHKAI G_wGCw'C
Cuts at: 2142 2274 2652 3435 3520 4591 2142
Size: 132 378 783 85 1071 2925
Fragments arranged by size:
2925 1071 783 378 132 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2175 2274 2341 2652 2693 3435
Size: 399 352 33 99 67 311 41 742
Cuts at: 3435 3520 4591 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 311 99 85
67 41 33
BspEI T'CCGG_A
Cuts at: 2281 5037 2281
Size: 2756 2618
BspHI T'CATG_A
Cuts at: 2034 3263 4993 2034
Size: 1229 1730 2415
Fragments arranged by size:
2415 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2188 4018 4205 1700
Size: 110 378 1830 187 2869
Fragments arranged by size:
2869 1830 378 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 2651 3434 4446 2651
Size: 783 1012 3579
Fragments arranged by size:
3579 1012 783
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5374
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2241 2574 2661 513
Size: 552 939 237 333 87 3226
Fragments arranged by size:
3226 939 552 333 237 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3684 3704 2890
Size: 794 20 4560
Fragments arranged by size:
4560 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2190 2244
Size: 54 1425 50 275 18 321 39 54
Cuts at: 2244 2821 3710 5339 8
Size: 577 889 1629 43
Fragments arranged by size:
1629 1425 889 577 321 275 54 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2190 2244 2821 1537
Size: 275 378 54 577 4090
Fragments arranged by size:
4090 577 378 275 54
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 3304 1353
Size: 1089 862 3423
Fragments arranged by size:
3423 1089 862
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2317 2645 3961 4335 4481 387
Size: 1064 138 728 328 1316 374 146 1280
Fragments arranged by size:
1316 1280 1064 728 374 328 146 138
EcoICRI GAG'CTC
Cuts at: 2140 2140
Size: 5374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3437 4034 4821 5089 1239
Size: 393 1805 597 787 268 1524
Fragments arranged by size:
1805 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2164 2164
Size: 5374
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 2126 3880 2126
Size: 1754 3620
HaeII r_GCGC'y
Cuts at: 1678 2133 2309 2514 2548 2594 4521 1678
Size: 455 176 205 34 46 1927 2531
Fragments arranged by size:
2531 1927 455 205 176 46 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 4015 4047 4089
Size: 32 135 32 872 32 1539 32 42
Cuts at: 4089 4121 1373
Size: 32 2626
Fragments arranged by size:
2626 1539 872 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KasI G'GCGC_C
Cuts at: 2305 2510 2590 2305
Size: 205 80 5089
Fragments arranged by size:
5089 205 80
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2609 4488 4672 1305
Size: 1304 1879 184 2007
Fragments arranged by size:
2007 1879 1304 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3552
Fragments arranged by size:
3552 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3332 3691 3850 518
Size: 648 843 1323 359 159 2042
Fragments arranged by size:
2042 1323 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2576 2790 3467 4615 4860 1606
Size: 504 466 214 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 504 466 245 214
NaeI GCC'GGC
Cuts at: 2190 2190
Size: 5374
NarI GG'CG_CC
Cuts at: 2306 2511 2591 2306
Size: 205 80 5089
Fragments arranged by size:
5089 205 80
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3883
Fragments arranged by size:
3883 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NgoMIV G'CCGG_C
Cuts at: 2188 2188
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2074 2641 2732 4277 1132
Size: 97 845 567 91 1545 2229
Fragments arranged by size:
2229 1545 845 567 97 91
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5374
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PspXI vC'TCGA_Gb
Cuts at: 2168 2168
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2109 2487 2541 2570 2726 830
Size: 1279 378 54 29 156 3478
Fragments arranged by size:
3478 1279 378 156 54 29
PvuI CG_AT'CG
Cuts at: 2001 2479 2001
Size: 478 4896
PvuII CAG'CTG
Cuts at: 2110 2110
Size: 5374
SacI G_AGCT'C
Cuts at: 2142 2142
Size: 5374
SacII CC_GC'GG
Cuts at: 2577 2577
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2105 2483 2537 2566 2722 3857 4538
Size: 219 1060 378 54 29 156 1135 681
Cuts at: 4538 4729 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1060 681 378 219 191 156
54 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2666 2666
Size: 5374
SfoI GGC'GCC
Cuts at: 2307 2512 2592 2307
Size: 205 80 5089
Fragments arranged by size:
5089 205 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2135 2701 1872
Size: 263 566 4545
Fragments arranged by size:
4545 566 263
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 3478 4379 4641 820
Size: 197 926 225 1310 901 262 1553
Fragments arranged by size:
1553 1310 926 901 262 225 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2074 2641 2732 1132
Size: 942 567 91 3774
Fragments arranged by size:
3774 942 567 91
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 2813 513
Size: 552 939 150 659 3074
Fragments arranged by size:
3074 939 659 552 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3418 3571 3756 4201 325
Size: 1255 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 5332 96
Size: 274 80 33 51 1443 1643 1712 138
Fragments arranged by size:
1712 1643 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5334 5366 5334
Size: 32 5342
Tth111I GACn'n_nGTC
Cuts at: 1316 2041 1316
Size: 725 4649
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 2168 1943
Size: 225 5149
XmaI C'CCGG_G
Cuts at: 1870 2133 2699 1870
Size: 263 566 4545
Fragments arranged by size:
4545 566 263
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PsrI RsrII SanDI SapI SexAI
SrfI SwaI XcmI