Restriction map for
pFN21AB9615
(Circular) (Six-base) MAPSORT of: pFN21AB9615.seq Check: 3913 from: 1 to: 5497
pFN21AB9615 5497 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2197 2481 2197
Size: 284 5213
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2683 2818 2683
Size: 135 5362
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2316 2441 3560 4944 5212 1632
Size: 684 125 1119 1384 268 1917
Fragments arranged by size:
1917 1384 1119 684 268 125
AfeI AGC'GCT
Cuts at: 2462 2462
Size: 5497
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 2309 2711 3240 4812 2309
Size: 402 529 1572 2994
Fragments arranged by size:
2994 1572 529 402
ApaLI G'TGCA_C
Cuts at: 2576 2694 3554 4710 2576
Size: 118 860 1156 3363
Fragments arranged by size:
3363 1156 860 118
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2088 2581 2822 1870
Size: 73 102 43 493 241 4545
Fragments arranged by size:
4545 493 241 102 73 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2675 2708 2675
Size: 33 5464
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2646 2683 2818 4273 618
Size: 325 217 627 859 37 135 1455 1842
Fragments arranged by size:
1842 1455 859 627 325 217 135 37
BanII G_rGCy'C
Cuts at: 1391 2196 2324 2816 1391
Size: 805 128 492 4072
Fragments arranged by size:
4072 805 492 128
BbeI G_GCGC'C
Cuts at: 2650 2650
Size: 5497
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2552 2586 3688 3722 1903
Size: 34 615 34 1102 34 3678
Fragments arranged by size:
3678 1102 615 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 2269 1548
Size: 721 4776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 4108 136
Size: 107 122 71 631 605 428 2008 1525
Fragments arranged by size:
2008 1525 631 605 428 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2914 2914
Size: 5497
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2531 2580 2606 2698 3558 4714 1790
Size: 493 248 49 26 92 860 1156 2573
Fragments arranged by size:
2573 1156 860 493 248 92 49 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2222 3228 4186
Size: 416 53 83 399 24 805 1006 958
Cuts at: 4186 442
Size: 1753
Fragments arranged by size:
1753 1006 958 805 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2449 4157 1239
Size: 1172 38 1708 2579
Fragments arranged by size:
2579 1708 1172 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 3622 4487 4785 2126
Size: 1496 865 298 2838
Fragments arranged by size:
2838 1496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2296 4160 882
Size: 617 797 1864 2219
Fragments arranged by size:
2219 1864 797 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2647 3304 3686 4321
Size: 53 83 186 936 1114 657 382 635
Cuts at: 4321 275
Size: 1451
Fragments arranged by size:
1451 1114 936 657 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 1178
Size: 30 165 30 1176 30 4066
Fragments arranged by size:
4066 1176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2067 2116 2246 2744 2780 2783 1186
Size: 881 49 130 498 36 3 3900
Fragments arranged by size:
3900 881 498 130 49 36 3
BseYI C'CCAG_C
Cuts at: 2015 2309 4700 5196 2015
Size: 294 2391 496 2316
Fragments arranged by size:
2391 2316 496 294
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 2231 1163
Size: 573 489 6 4429
Fragments arranged by size:
4429 573 489 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2796 2947 3708 4736 1540
Size: 275 186 795 151 761 1028 2301
Fragments arranged by size:
2301 1028 795 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2580 2698 3558 3643 4714 2580
Size: 118 860 85 1071 3363
Fragments arranged by size:
3363 1071 860 118 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2283 2324 2531 2580 2606 2698
Size: 399 406 87 41 207 49 26 92
Cuts at: 2698 2816 3558 3643 4714 1391
Size: 118 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 207 118 92
87 85 49 41 26
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2197 2481 2852 844
Size: 1353 284 371 3489
Fragments arranged by size:
3489 1353 371 284
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2498 2791 4141 4328 1700
Size: 110 688 293 1350 187 2869
Fragments arranged by size:
2869 1350 688 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2442 794
Size: 1648 3849
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2701 2827 3194 3579 3596 5048 5134 5146
Size: 729 126 367 385 17 1452 86 12
Cuts at: 5146 5492 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 729 385 367 346 126 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2316 2316
Size: 5497
BtgI C'CryG_G
Cuts at: 513 1065 2004 2676 2720 513
Size: 552 939 672 44 3290
Fragments arranged by size:
3290 939 672 552 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2555 487
Size: 683 56 1329 3429
Fragments arranged by size:
3429 1329 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 2482 3013 3807 3827 2245
Size: 237 531 794 20 3915
Fragments arranged by size:
3915 794 531 237 20
ClaI AT'CG_AT
Cuts at: 1554 2265 1554
Size: 711 4786
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2793 2944 3833
Size: 54 1425 50 275 18 963 151 889
Cuts at: 3833 5462 8
Size: 1629 43
Fragments arranged by size:
1629 1425 963 889 275 151 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2793 2944 1537
Size: 275 981 151 4090
Fragments arranged by size:
4090 981 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2757 3427 1353
Size: 696 708 670 3423
Fragments arranged by size:
3423 708 696 670
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2411 2441 2449 3560 4157 4944
Size: 393 684 95 30 8 1111 597 787
Cuts at: 4944 5212 1239
Size: 268 1524
Fragments arranged by size:
1524 1111 787 684 597 393 268 95
30 8
EcoO109I rG'GnC_Cy
Cuts at: 2338 2338
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2398 2464 2593 2650 4644 1678
Size: 720 66 129 57 1994 2531
Fragments arranged by size:
2531 1994 720 129 66 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2254 2286 2693 2725 4138
Size: 32 135 32 682 32 407 32 1413
Cuts at: 4138 4170 4212 4244 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1413 682 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KasI G'GCGC_C
Cuts at: 2646 2646
Size: 5497
KpnI G_GTAC'C
Cuts at: 2687 2822 2687
Size: 135 5362
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 4611 4795 1305
Size: 1104 2202 184 2007
Fragments arranged by size:
2202 2007 1104 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3675
Fragments arranged by size:
3675 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3455 3814 3973 518
Size: 648 2289 359 159 2042
Fragments arranged by size:
2289 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2306 2517 2524 2913 3590 4738 4983 1606
Size: 700 211 7 389 677 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 389 245 211 7
NaeI GCC'GGC
Cuts at: 2500 2793 2500
Size: 293 5204
NarI GG'CG_CC
Cuts at: 2647 2647
Size: 5497
NcoI C'CATG_G
Cuts at: 513 1065 2004 2676 513
Size: 552 939 672 3334
Fragments arranged by size:
3334 939 672 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NgoMIV G'CCGG_C
Cuts at: 2498 2791 2498
Size: 293 5204
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2849 830
Size: 2019 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2845 3980 4661 4852 826
Size: 219 1800 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2100 2100
Size: 5497
SfoI GGC'GCC
Cuts at: 2648 2648
Size: 5497
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2047 2090 2824 1872
Size: 175 43 734 4545
Fragments arranged by size:
4545 734 175 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 3601 4502 4764 820
Size: 197 926 198 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 198 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 1517
Size: 630 4867
StyI C'CwwG_G
Cuts at: 513 1065 2004 2676 2936 513
Size: 552 939 672 260 3074
Fragments arranged by size:
3074 939 672 552 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3524 3541 3694 3879 4324 325
Size: 1255 1944 17 153 185 445 1498
Fragments arranged by size:
1944 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2045 2088 2822 1870
Size: 175 43 734 4545
Fragments arranged by size:
4545 734 175 43
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SrfI SwaI XcmI