Restriction map for
pFN21AB9614
(Circular) (Six-base) MAPSORT of: pFN21AB9614.seq Check: 3466 from: 1 to: 6211
pFN21AB9614 6211 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3554 1937
Size: 1617 4594
Acc65I G'GTAC_C
Cuts at: 3532 3532
Size: 6211
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2070 2689 2853 3109 4274 5658 5926 1632
Size: 438 619 164 256 1165 1384 268 1917
Fragments arranged by size:
1917 1384 1165 619 438 268 256 164
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 2139 2429 5110 6087 1203
Size: 936 290 2681 977 1327
Fragments arranged by size:
2681 1327 977 936 290
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 3462 4940 3462
Size: 1478 4733
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6211
AlwNI CAG_nnn'CTG
Cuts at: 3954 5526 3954
Size: 1572 4639
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 2043 2084 2571 2655 2713 2823 2907 2991
Size: 550 41 487 84 58 110 84 84
Cuts at: 2991 3075 3159 3243 3327 3411 3520 3746 3898
Size: 84 84 84 84 84 109 226 152
Cuts at: 3898 6007 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 550 487 226 152 110 109
84 84 84 84 84 84 84 84
58 41
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 3536 1870
Size: 73 1593 4545
Fragments arranged by size:
4545 1593 73
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2562 3532 4987 618
Size: 325 217 627 775 970 1455 1842
Fragments arranged by size:
1842 1455 970 775 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3473 3530 1391
Size: 2082 57 4072
Fragments arranged by size:
4072 2082 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2142 2789 3461 928
Size: 971 243 647 672 3678
Fragments arranged by size:
3678 971 672 647 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4402 4436 1903
Size: 34 2465 34 3678
Fragments arranged by size:
3678 2465 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4822 136
Size: 107 122 71 631 605 3150 1525
Fragments arranged by size:
3150 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 3628 3628
Size: 6211
Bme1580I G_kGCm'C
Cuts at: 1790 4272 5428 1790
Size: 2482 1156 2573
Fragments arranged by size:
2573 2482 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3942 4900 442
Size: 416 53 83 399 24 2525 958 1753
Fragments arranged by size:
2525 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2601 2685 2769 2937 3189 3273 3441 4871
Size: 1362 84 84 168 252 84 168 1430
Cuts at: 4871 1239
Size: 2579
Fragments arranged by size:
2579 1430 1362 252 168 168 84 84
84
Bpu10I CC'TnA_GC
Cuts at: 2884 2884
Size: 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 4336 5201 5499 2230
Size: 2106 865 298 2942
Fragments arranged by size:
2942 2106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2665 4874 882
Size: 617 1166 2209 2219
Fragments arranged by size:
2219 2209 1166 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 6205 6205
Size: 6211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4018 4400 5035 275
Size: 53 83 186 936 2485 382 635 1451
Fragments arranged by size:
2485 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4644 5042 5316 5463 5874 4644
Size: 398 274 147 411 4981
Fragments arranged by size:
4981 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5986
Fragments arranged by size:
5986 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2184 3470 1186
Size: 998 1286 3927
Fragments arranged by size:
3927 1286 998
BseYI C'CCAG_C
Cuts at: 2294 5414 5910 2294
Size: 3120 496 2595
Fragments arranged by size:
3120 2595 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5638
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3473 4272 4357 5428 3473
Size: 799 85 1071 4256
Fragments arranged by size:
4256 1071 799 85
BsmI GAATG_Cn'
Cuts at: 2855 3191 3385 3722 3815 2855
Size: 336 194 337 93 5251
Fragments arranged by size:
5251 337 336 194 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3473 3530 4272 4357 5428 1391
Size: 399 1683 57 742 85 1071 2174
Fragments arranged by size:
2174 1683 1071 742 399 85 57
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 2110 4100 5830 2110
Size: 1990 1730 2491
Fragments arranged by size:
2491 1990 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3566 844
Size: 2722 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2860 4706 4880 65
Size: 1183 1612 1846 174 1396
Fragments arranged by size:
1846 1612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6211
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206
Size: 1569 367 385 17 1452 86 12 346
Cuts at: 6206 1972
Size: 1977
Fragments arranged by size:
1977 1569 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2196 2196
Size: 6211
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 4558
Fragments arranged by size:
4558 939 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 3727 4521 4541 3727
Size: 794 20 5397
Fragments arranged by size:
5397 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2842 2877 529
Size: 35 2278 35 3863
Fragments arranged by size:
3863 2278 35 35
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2359 3043 3658
Size: 54 1425 50 275 18 529 684 615
Cuts at: 3658 4547 6176 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 684 615 529 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 4141 1353
Size: 813 1975 3423
Fragments arranged by size:
3423 1975 813
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3229 4798 5172 5318 387
Size: 1064 138 1640 1569 374 146 1280
Fragments arranged by size:
1640 1569 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3471 3471
Size: 6211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 2601 2685 2689 2769 2853 2937
Size: 393 438 531 84 4 80 84 84
Cuts at: 2937 3109 3189 3273 3441 4274 4871 5658 5926
Size: 172 80 84 168 833 597 787 268
Cuts at: 5926 1239
Size: 1524
Fragments arranged by size:
1524 833 787 597 531 438 393 268
172 168 84 84 84 84 80 80
4
EcoRI G'AATT_C
Cuts at: 2571 2655 2823 2991 3075 3159 3243 3411 3520
Size: 84 168 168 84 84 84 168 109
Cuts at: 3520 3898 2571
Size: 378 4884
Fragments arranged by size:
4884 378 168 168 168 109 84 84
84 84
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 5358 1678
Size: 3680 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2179 2198 2206 2211
Size: 32 135 32 602 5 19 8 5
Cuts at: 2211 2230 3448 3480 4852 4884 4926 4958 1373
Size: 19 1218 32 1372 32 42 32 2626
Fragments arranged by size:
2626 1372 1218 602 135 42 32 32
32 32 32 19 19 8 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3555 3801 669
Size: 988 281 1617 246 3079
Fragments arranged by size:
3079 1617 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KpnI G_GTAC'C
Cuts at: 3536 3536
Size: 6211
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 3446 3508 5325 5509 1305
Size: 819 1322 62 1817 184 2007
Fragments arranged by size:
2007 1817 1322 819 184 62
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2361 3045 10
Size: 54 1425 343 529 684 3176
Fragments arranged by size:
3176 1425 684 529 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2764 2932 3084 3492 4169 4528 4687
Size: 648 1598 168 152 408 677 359 159
Cuts at: 4687 518
Size: 2042
Fragments arranged by size:
2042 1598 677 648 408 359 168 159
152
MspA1I CmG'CkG
Cuts at: 1606 3627 4304 5452 5697 1606
Size: 2021 677 1148 245 2120
Fragments arranged by size:
2120 2021 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 4558
Fragments arranged by size:
4558 939 552 162
NdeI CA'TA_TG
Cuts at: 387 2481 2761 387
Size: 2094 280 3837
Fragments arranged by size:
3837 2094 280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 2143 2433 3569 5114 1132
Size: 97 914 290 1136 1545 2229
Fragments arranged by size:
2229 1545 1136 914 290 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6211
PciI A'CATG_T
Cuts at: 2139 2429 5110 2139
Size: 290 2681 3240
Fragments arranged by size:
3240 2681 290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6211
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PstI C_TGCA'G
Cuts at: 830 2291 3563 830
Size: 1461 1272 3478
Fragments arranged by size:
3478 1461 1272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
SacI G_AGCT'C
Cuts at: 3473 3473
Size: 6211
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SfcI C'TryA_G
Cuts at: 826 1045 2287 3559 4694 5375 5566 826
Size: 219 1242 1272 1135 681 191 1471
Fragments arranged by size:
1471 1272 1242 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3538 1872
Size: 1666 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2245 4315 5216 5478 820
Size: 197 926 302 2070 901 262 1553
Fragments arranged by size:
2070 1553 926 901 302 262 197
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 4135 5
Size: 47 4083 2081
Fragments arranged by size:
4083 2081 47
StuI AGG'CCT
Cuts at: 1517 2618 2889 3122 1517
Size: 1101 271 233 4606
Fragments arranged by size:
4606 1101 271 233
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2104 2166 3650 513
Size: 552 939 30 70 62 1484 3074
Fragments arranged by size:
3074 1484 939 552 70 62 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4238 4255 4408 4593 5038 325
Size: 1255 2658 17 153 185 445 1498
Fragments arranged by size:
2658 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4457 6169 96
Size: 274 80 33 51 1443 2480 1712 138
Fragments arranged by size:
2480 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 6171 6203 2457
Size: 32 3682 32 2465
Fragments arranged by size:
3682 2465 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 2302
Size: 6211
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3536 1870
Size: 1666 4545
XmnI GAAnn'nnTTC
Cuts at: 2085 2545 2954 3122 4340 2085
Size: 460 409 168 1218 3956
Fragments arranged by size:
3956 1218 460 409 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI