Restriction map for
pFN21AB9612
(Circular) (Six-base) MAPSORT of: pFN21AB9612.seq Check: 8909 from: 1 to: 6778
pFN21AB9612 6778 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4588 278
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4121 1937
Size: 2184 4594
Acc65I G'GTAC_C
Cuts at: 4099 4099
Size: 6778
AclI AA'CG_TT
Cuts at: 1563 4905 5278 1563
Size: 3342 373 3063
Fragments arranged by size:
3342 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 2720 3359 4841 6225 6493 1632
Size: 671 417 639 1482 1384 268 1917
Fragments arranged by size:
1917 1482 1384 671 639 417 268
AflII C'TTAA_G
Cuts at: 820 1017 3032 820
Size: 197 2015 4566
Fragments arranged by size:
4566 2015 197
AflIII A'CryG_T
Cuts at: 1203 5677 6654 1203
Size: 4474 977 1327
Fragments arranged by size:
4474 1327 977
AgeI A'CCGG_T
Cuts at: 5609 5609
Size: 6778
AhdI GACnn_n'nnGTC
Cuts at: 5507 5507
Size: 6778
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3622 1184
Size: 2438 4340
AlwNI CAG_nnn'CTG
Cuts at: 4521 6093 4521
Size: 1572 5206
ApaLI G'TGCA_C
Cuts at: 4835 5991 4835
Size: 1156 5622
ApoI r'AATT_y
Cuts at: 1493 2967 3256 3350 3617 4087 4313 4465 6574
Size: 1474 289 94 267 470 226 152 2109
Cuts at: 6574 1493
Size: 1697
Fragments arranged by size:
2109 1697 1474 470 289 267 226 152
94
AseI AT'TA_AT
Cuts at: 160 1032 5332 160
Size: 872 4300 1606
Fragments arranged by size:
4300 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
AvaI C'yCGr_G
Cuts at: 1870 1943 4103 1870
Size: 73 2160 4545
Fragments arranged by size:
4545 2160 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3362 3395 3362
Size: 33 6745
BamHI G'GATC_C
Cuts at: 4108 4475 4108
Size: 367 6411
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4099 5554 618
Size: 325 217 627 2312 1455 1842
Fragments arranged by size:
2312 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2743 3137 4097 1391
Size: 1352 394 960 4072
Fragments arranged by size:
4072 1352 960 394
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2291 3785 3862 928
Size: 971 392 1494 77 3844
Fragments arranged by size:
3844 1494 971 392 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4969 5003 1903
Size: 34 3032 34 3678
Fragments arranged by size:
3678 3032 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4670 5880 4670
Size: 1210 5568
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6778
BfrBI ATG'CAT
Cuts at: 2486 3599 2486
Size: 1113 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5389 136
Size: 107 122 71 631 605 337 3380 1525
Fragments arranged by size:
3380 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6773 6773
Size: 6778
BlpI GC'TnA_GC
Cuts at: 4195 4195
Size: 6778
Bme1580I G_kGCm'C
Cuts at: 1790 4839 5995 1790
Size: 3049 1156 2573
Fragments arranged by size:
3049 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6778
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4509 5467 442
Size: 416 53 83 399 24 3092 958 1753
Fragments arranged by size:
3092 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6778
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3151 3151
Size: 6778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2378 5438 1239
Size: 995 144 3060 2579
Fragments arranged by size:
3060 2579 995 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3121 4903 5768 6066 3121
Size: 1782 865 298 3833
Fragments arranged by size:
3833 1782 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3721 5441 882
Size: 617 2222 1720 2219
Fragments arranged by size:
2222 2219 1720 617
BsaAI yAC'GTr
Cuts at: 493 1204 3410 4569 493
Size: 711 2206 1159 2702
Fragments arranged by size:
2702 2206 1159 711
BsaBI GATnn'nnATC
Cuts at: 3913 6772 3913
Size: 2859 3919
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4585 4967 5602 275
Size: 53 83 186 936 3052 382 635 1451
Fragments arranged by size:
3052 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5211 5609 5883 6030 6441 5211
Size: 398 274 147 411 5548
Fragments arranged by size:
5548 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6553
Fragments arranged by size:
6553 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2855 3990 1186
Size: 1669 1135 3974
Fragments arranged by size:
3974 1669 1135
BseYI C'CCAG_C
Cuts at: 5981 6477 5981
Size: 496 6282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6205
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4228 4989 6017 1540
Size: 275 186 2227 761 1028 2301
Fragments arranged by size:
2301 2227 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2438 3137 4839 4924 5995 2438
Size: 699 1702 85 1071 3221
Fragments arranged by size:
3221 1702 1071 699 85
BsmI GAATG_Cn'
Cuts at: 3971 4289 4382 3971
Size: 318 93 6367
Fragments arranged by size:
6367 318 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2438 2743 3137 4097 4839 4924 5995
Size: 399 648 305 394 960 742 85 1071
Cuts at: 5995 1391
Size: 2174
Fragments arranged by size:
2174 1071 960 742 648 399 394 305
85
BspEI T'CCGG_A
Cuts at: 6441 6441
Size: 6778
BspHI T'CATG_A
Cuts at: 4667 6397 4667
Size: 1730 5048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2967 4133 844
Size: 2123 1166 3489
Fragments arranged by size:
3489 2123 1166
BsrBI CCG'CTC
Cuts at: 1394 1823 4665 1394
Size: 429 2842 3507
Fragments arranged by size:
3507 2842 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5273 5447 65
Size: 1183 4025 174 1396
Fragments arranged by size:
4025 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5422 5609 1700
Size: 110 3612 187 2869
Fragments arranged by size:
3612 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2489 96
Size: 2393 4385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6778
BssSI C'ACGA_G
Cuts at: 4838 5850 4838
Size: 1012 5766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6778
BstBI TT'CG_AA
Cuts at: 5582 5582
Size: 6778
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6778
BstXI CCAn_nnnn'nTGG
Cuts at: 3237 3237
Size: 6778
BstYI r'GATC_y
Cuts at: 1972 4108 4475 4860 4877 6329 6415 6427 6773
Size: 2136 367 385 17 1452 86 12 346
Cuts at: 6773 1972
Size: 1977
Fragments arranged by size:
2136 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6039
Fragments arranged by size:
6039 683 56
BtsI GCAGTG_nn'
Cuts at: 3892 4294 5088 5108 3892
Size: 402 794 20 5562
Fragments arranged by size:
5562 794 402 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6743
DraI TTT'AAA
Cuts at: 2025 2248 2275 2569 3088 4033 4082 2025
Size: 223 27 294 519 945 49 4721
Fragments arranged by size:
4721 945 519 294 223 49 27
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5785 809
Size: 507 4469 1802
Fragments arranged by size:
4469 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3024 4225 5114
Size: 54 1425 50 275 18 1194 1201 889
Cuts at: 5114 6743 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1201 1194 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4225 1537
Size: 275 2413 4090
Fragments arranged by size:
4090 2413 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2750 4004 4708 1353
Size: 1397 1254 704 3423
Fragments arranged by size:
3423 1397 1254 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5365 5739 5885 387
Size: 1064 138 3776 374 146 1280
Fragments arranged by size:
3776 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3135 3135
Size: 6778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2303 2378 2720 3359 4841 5438
Size: 393 602 69 75 342 639 1482 597
Cuts at: 5438 6225 6493 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1482 787 639 602 597 393 342
268 75 69
EcoO109I rG'GnC_Cy
Cuts at: 3507 3507
Size: 6778
EcoRI G'AATT_C
Cuts at: 3350 4087 4465 3350
Size: 737 378 5663
Fragments arranged by size:
5663 737 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3115 3287 3337 3846 3115
Size: 172 50 509 6047
Fragments arranged by size:
6047 509 172 50
FspI TGC'GCA
Cuts at: 5284 5284
Size: 6778
HaeII r_GCGC'y
Cuts at: 1678 5925 1678
Size: 4247 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2849 2881 3719 3751 5419
Size: 32 135 32 1277 32 838 32 1668
Cuts at: 5419 5451 5493 5525 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1668 1277 838 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2604 3664 4122 4368 669
Size: 988 281 666 1060 458 246 3079
Fragments arranged by size:
3079 1060 988 666 458 281 246
HindIII A'AGCT_T
Cuts at: 748 3029 748
Size: 2281 4497
HpaI GTT'AAC
Cuts at: 3664 4368 3664
Size: 704 6074
KpnI G_GTAC'C
Cuts at: 4103 4103
Size: 6778
MfeI C'AATT_G
Cuts at: 3195 4377 3195
Size: 1182 5596
MluI A'CGCG_T
Cuts at: 6654 6654
Size: 6778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5892 6076 1305
Size: 4587 184 2007
Fragments arranged by size:
4587 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3026 10
Size: 54 1425 343 1194 3762
Fragments arranged by size:
3762 1425 1194 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2170 3964 4736 5095 5254 518
Size: 648 1004 1794 772 359 159 2042
Fragments arranged by size:
2042 1794 1004 772 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2943 4194 4871 6019 6264 1606
Size: 1337 1251 677 1148 245 2120
Fragments arranged by size:
2120 1337 1251 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6226
NdeI CA'TA_TG
Cuts at: 387 2083 387
Size: 1696 5082
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6778
NotI GC'GGCC_GC
Cuts at: 4225 4225
Size: 6778
NsiI A_TGCA'T
Cuts at: 2488 3601 2488
Size: 1113 5665
NspI r_CATG'y
Cuts at: 1132 1229 2490 2540 4136 5681 1132
Size: 97 1261 50 1596 1545 2229
Fragments arranged by size:
2229 1596 1545 1261 97 50
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6778
PciI A'CATG_T
Cuts at: 5677 5677
Size: 6778
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6778
PmeI GTTT'AAAC
Cuts at: 4082 4082
Size: 6778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4847 4879 6360 6392 4847
Size: 32 1481 32 5233
Fragments arranged by size:
5233 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6778
PsiI TTA'TAA
Cuts at: 4348 4348
Size: 6778
PstI C_TGCA'G
Cuts at: 830 4130 830
Size: 3300 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6778
PvuII CAG'CTG
Cuts at: 2943 2943
Size: 6778
SacI G_AGCT'C
Cuts at: 3137 3137
Size: 6778
SalI G'TCGA_C
Cuts at: 1936 4120 1936
Size: 2184 4594
SbfI CC_TGCA'GG
Cuts at: 4130 4130
Size: 6778
ScaI AGT'ACT
Cuts at: 5026 6738 5026
Size: 1712 5066
SfcI C'TryA_G
Cuts at: 826 1045 4126 5261 5942 6133 826
Size: 219 3081 1135 681 191 1471
Fragments arranged by size:
3081 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6778
SmaI CCC'GGG
Cuts at: 1872 4105 1872
Size: 2233 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 3100 4882 5783 6045 820
Size: 197 926 1089 68 1782 901 262 1553
Fragments arranged by size:
1782 1553 1089 926 901 262 197 68
SnaBI TAC'GTA
Cuts at: 493 3410 4569 493
Size: 2917 1159 2702
Fragments arranged by size:
2917 2702 1159
SpeI A'CTAG_T
Cuts at: 152 2203 152
Size: 2051 4727
SphI G_CATG'C
Cuts at: 1132 4136 1132
Size: 3004 3774
SspI AAT'ATT
Cuts at: 5 52 2095 2479 3523 4702 5
Size: 47 2043 384 1044 1179 2081
Fragments arranged by size:
2081 2043 1179 1044 384 47
StuI AGG'CCT
Cuts at: 1517 2563 1517
Size: 1046 5732
StyI C'CwwG_G
Cuts at: 513 1065 3395 4217 513
Size: 552 2330 822 3074
Fragments arranged by size:
3074 2330 822 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4805 4822 4975 5160 5605 325
Size: 1255 3225 17 153 185 445 1498
Fragments arranged by size:
3225 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2489 2539 2582
Size: 274 80 33 51 1443 512 50 43
Cuts at: 2582 3802 5024 6736 96
Size: 1220 1222 1712 138
Fragments arranged by size:
1712 1443 1222 1220 512 274 138 80
51 50 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 6738 6770 2844
Size: 32 3862 32 2852
Fragments arranged by size:
3862 2852 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6778
XbaI T'CTAG_A
Cuts at: 4114 4114
Size: 6778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3617 3617
Size: 6778
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6778
XmaI C'CCGG_G
Cuts at: 1870 4103 1870
Size: 2233 4545
XmnI GAAnn'nnTTC
Cuts at: 4907 4907
Size: 6778
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4586 276
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI