Restriction map for
pFN21AB9608
(Circular) (Six-base) MAPSORT of: pFN21AB9608.seq Check: 7152 from: 1 to: 5557
pFN21AB9608 5557 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2049 2049
Size: 5557
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2518 2602 2686 3620 5004 5272 1632
Size: 886 84 84 934 1384 268 1917
Fragments arranged by size:
1917 1384 934 886 268 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 4456 5433 1203
Size: 3253 977 1327
Fragments arranged by size:
3253 1327 977
AgeI A'CCGG_T
Cuts at: 2760 4388 2760
Size: 1628 3929
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 2325 2661 1166
Size: 1159 336 4062
Fragments arranged by size:
4062 1159 336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 3300 4872 3300
Size: 1572 3985
ApaLI G'TGCA_C
Cuts at: 2680 2764 3614 4770 2680
Size: 84 850 1156 3467
Fragments arranged by size:
3467 1156 850 84
ApoI r'AATT_y
Cuts at: 1493 2866 3092 3244 5353 1493
Size: 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2368 2882 1870
Size: 73 256 169 514 4545
Fragments arranged by size:
4545 514 256 169 73
AvrII C'CTAG_G
Cuts at: 2491 2491
Size: 5557
BamHI G'GATC_C
Cuts at: 2100 2316 2400 2887 3254 2100
Size: 216 84 487 367 4403
Fragments arranged by size:
4403 487 367 216 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2878 4333 618
Size: 325 217 627 1091 1455 1842
Fragments arranged by size:
1842 1455 1091 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2200 2798 2876 1391
Size: 809 598 78 4072
Fragments arranged by size:
4072 809 598 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4168 136
Size: 107 122 71 631 605 2496 1525
Fragments arranged by size:
2496 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2684 2768 3618 4774 1790
Size: 476 418 84 850 1156 2573
Fragments arranged by size:
2573 1156 850 476 418 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 3288 4246
Size: 416 53 83 399 24 843 1028 958
Cuts at: 4246 442
Size: 1753
Fragments arranged by size:
1753 1028 958 843 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2555 1056
Size: 1499 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2597 2614 4217 1239
Size: 1358 17 1603 2579
Fragments arranged by size:
2579 1603 1358 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2662 4220 882
Size: 617 1163 1558 2219
Fragments arranged by size:
2219 1558 1163 617
BsaAI yAC'GTr
Cuts at: 493 1204 3348 493
Size: 711 2144 2702
Fragments arranged by size:
2702 2144 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3364 3746 4381 275
Size: 53 83 186 936 1831 382 635 1451
Fragments arranged by size:
1831 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2565 2760 3990 4388 4662 4809 5220 2565
Size: 195 1230 398 274 147 411 2902
Fragments arranged by size:
2902 1230 411 398 274 195 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5332
Fragments arranged by size:
5332 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5557
BseYI C'CCAG_C
Cuts at: 2840 4760 5256 2840
Size: 1920 496 3141
Fragments arranged by size:
3141 1920 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4984
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 3768 4796 1540
Size: 275 186 1006 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2684 2728 2768 2798 3618 3703 4774 2684
Size: 44 40 30 820 85 1071 3467
Fragments arranged by size:
3467 1071 820 85 44 40 30
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2200 2266 2684 2728 2768 2798 2876
Size: 399 410 66 418 44 40 30 78
Cuts at: 2876 3618 3703 4774 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 418 410 399 85 78
66 44 40 30
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 2912 844
Size: 1205 863 3489
Fragments arranged by size:
3489 1205 863
BsrBI CCG'CTC
Cuts at: 1394 1823 2398 3444 1394
Size: 429 575 1046 3507
Fragments arranged by size:
3507 1046 575 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2217 4052 4226 65
Size: 1183 969 1835 174 1396
Fragments arranged by size:
1835 1396 1183 969 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 4201 4388 1700
Size: 110 950 1441 187 2869
Fragments arranged by size:
2869 1441 950 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 2393 3617 4629 2393
Size: 1224 1012 3321
Fragments arranged by size:
3321 1224 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2100 2316 2400 2887 3254 3639 3656 5108
Size: 128 216 84 487 367 385 17 1452
Cuts at: 5108 5194 5206 5552 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 487 385 367 346 216 128
86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2046 2046
Size: 5557
BtgI C'CryG_G
Cuts at: 513 1065 2004 2174 513
Size: 552 939 170 3896
Fragments arranged by size:
3896 939 552 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 3073 3867 3887 2017
Size: 1056 794 20 3687
Fragments arranged by size:
3687 1056 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DraIII CAC_nnn'GTG
Cuts at: 2688 2772 2688
Size: 84 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2501 3004 3893
Size: 54 1425 50 275 18 671 503 889
Cuts at: 3893 5522 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 671 503 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3004 1537
Size: 275 1192 4090
Fragments arranged by size:
4090 1192 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 2606 3487 1353
Size: 1053 200 881 3423
Fragments arranged by size:
3423 1053 881 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2710 2798 4144 4518 4664 387
Size: 1064 138 1121 88 1346 374 146 1280
Fragments arranged by size:
1346 1280 1121 1064 374 146 138 88
EcoICRI GAG'CTC
Cuts at: 2796 2796
Size: 5557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2518 2597 2602 2614 2686 3620 4217
Size: 393 886 79 5 12 72 934 597
Cuts at: 4217 5004 5272 1239
Size: 787 268 1524
Fragments arranged by size:
1524 934 886 787 597 393 268 79
72 12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2049 2045
Size: 4 5553
EcoO109I rG'GnC_Cy
Cuts at: 2212 2212
Size: 5557
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
EcoRV GAT'ATC
Cuts at: 2710 2710
Size: 5557
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 2461 4704 1678
Size: 783 2243 2531
Fragments arranged by size:
2531 2243 783
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 2785 2817 4198
Size: 32 135 32 551 32 630 32 1381
Cuts at: 4198 4230 4272 4304 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1381 630 551 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2319 2403 2739 4671 4855 1305
Size: 816 198 84 336 1932 184 2007
Fragments arranged by size:
2007 1932 816 336 198 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3735
Fragments arranged by size:
3735 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2325 2661 3515 3874 4033 518
Size: 648 1159 336 854 359 159 2042
Fragments arranged by size:
2042 1159 854 648 359 336 159
MspA1I CmG'CkG
Cuts at: 1606 2314 2511 2734 2973 3650 4798 5043 1606
Size: 708 197 223 239 677 1148 245 2120
Fragments arranged by size:
2120 1148 708 677 245 239 223 197
NcoI C'CATG_G
Cuts at: 513 1065 2004 2174 513
Size: 552 939 170 3896
Fragments arranged by size:
3896 939 552 170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 2551 1052
Size: 1499 4058
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2915 4460 1132
Size: 97 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5557
PfoI T'CCnGG_A
Cuts at: 2789 2789
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2212
Size: 5557
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2909 830
Size: 2079 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
SacI G_AGCT'C
Cuts at: 2798 2798
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SanDI GG'GwC_CC
Cuts at: 2212 2212
Size: 5557
SapI GCTCTTCn'nnn_
Cuts at: 2606 2606
Size: 5557
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2905 4040 4721 4912 826
Size: 219 1860 1135 681 191 1471
Fragments arranged by size:
1860 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2884 1872
Size: 1012 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2199 2368 3661 4562 4824 820
Size: 197 926 256 169 1293 901 262 1553
Fragments arranged by size:
1553 1293 926 901 262 256 197 169
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2069 2174 2228 2491 2996 513
Size: 552 939 65 105 54 263 505 3074
Fragments arranged by size:
3074 939 552 505 263 105 65 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2669 3584 3601 3754 3939 4384 325
Size: 1255 1089 915 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1089 915 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5517 5549 5517
Size: 32 5525
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2172 2172
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 2199 2368 1943
Size: 256 169 5132
Fragments arranged by size:
5132 256 169
XmaI C'CCGG_G
Cuts at: 1870 2882 1870
Size: 1012 4545
XmnI GAAnn'nnTTC
Cuts at: 2615 2783 3686 2615
Size: 168 903 4486
Fragments arranged by size:
4486 903 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI
SfiI SfoI SrfI SwaI