Restriction map for
pFN21AB9606
(Circular) (Six-base) MAPSORT of: pFN21AB9606.seq Check: 7503 from: 1 to: 5782
pFN21AB9606 5782 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 2823 3103 2823
Size: 280 5502
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3845 5229 5497 1632
Size: 2213 1384 268 1917
Fragments arranged by size:
2213 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 2293 4511 2293
Size: 2218 3564
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2066 3022 3525 5097 2066
Size: 956 503 1572 2751
Fragments arranged by size:
2751 1572 956 503
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2342 3107 1870
Size: 73 183 216 765 4545
Fragments arranged by size:
4545 765 216 183 73
BamHI G'GATC_C
Cuts at: 2568 3112 3479 2568
Size: 544 367 4871
Fragments arranged by size:
4871 544 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2823 3103 4558 618
Size: 325 217 627 335 701 280 1455 1842
Fragments arranged by size:
1842 1455 701 627 335 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2401 2865 3101 1391
Size: 1010 464 236 4072
Fragments arranged by size:
4072 1010 464 236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2638 1548
Size: 1090 4692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2106 4393 136
Size: 107 122 71 631 605 434 2287 1525
Fragments arranged by size:
2287 1525 631 605 434 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2127 3843 4999 1790
Size: 337 1716 1156 2573
Fragments arranged by size:
2573 1716 1156 337
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 3513 4471
Size: 416 53 83 399 24 741 1355 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1355 958 741 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2906 2954 2999 3005 4442 1239
Size: 1667 48 45 6 1437 2579
Fragments arranged by size:
2579 1667 1437 48 45 6
Bpu10I CC'TnA_GC
Cuts at: 2094 2094
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2339 2470 3589 3971
Size: 53 83 186 936 806 131 1119 382
Cuts at: 3971 4606 275
Size: 635 1451
Fragments arranged by size:
1451 1119 936 806 635 382 186 131
83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5557
Fragments arranged by size:
5557 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2144 2435 2666 2759 1186
Size: 943 15 291 231 93 4209
Fragments arranged by size:
4209 943 291 231 93 15
BseYI C'CCAG_C
Cuts at: 2361 2446 2947 4985 5481 2361
Size: 85 501 2038 496 2662
Fragments arranged by size:
2662 2038 501 496 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3023 1163
Size: 573 1287 3922
Fragments arranged by size:
3922 1287 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2604 3232 3993 5021 1540
Size: 275 186 603 628 761 1028 2301
Fragments arranged by size:
2301 1028 761 628 603 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 3843 3928 4999 2865
Size: 978 85 1071 3648
Fragments arranged by size:
3648 1071 978 85
BsiWI C'GTAC_G
Cuts at: 2814 2814
Size: 5782
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
BsmBI CGTCTCn'nnnn_
Cuts at: 2461 2804 3072 2461
Size: 343 268 5171
Fragments arranged by size:
5171 343 268
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2401 2865 3101 3843 3928 4999
Size: 399 337 274 464 236 742 85 1071
Cuts at: 4999 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 464 399 337 274 236
85
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2371 3671 5401 2371
Size: 1300 1730 2752
Fragments arranged by size:
2752 1730 1300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3137 844
Size: 2293 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 2821 3669 1394
Size: 429 296 702 848 3507
Fragments arranged by size:
3507 848 702 429 296
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2320 2599 2669 2922 2994 3042 4426
Size: 110 510 279 70 253 72 48 1384
Cuts at: 4426 4613 1700
Size: 187 2869
Fragments arranged by size:
2869 1384 510 279 253 187 110 72
70 48
BsrGI T'GTAC_A
Cuts at: 96 2057 96
Size: 1961 3821
BssHII G'CGCG_C
Cuts at: 1925 3001 1925
Size: 1076 4706
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2171 2392 2171
Size: 221 5561
BstYI r'GATC_y
Cuts at: 1972 2568 2659 3112 3479 3864 3881 5333 5419
Size: 596 91 453 367 385 17 1452 86
Cuts at: 5419 5431 5777 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 596 453 385 367 346 91
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4291
Fragments arranged by size:
4291 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2730 2775 487
Size: 683 56 1504 45 3494
Fragments arranged by size:
3494 1504 683 56 45
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2205 2240 529
Size: 35 1641 35 4071
Fragments arranged by size:
4071 1641 35 35
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2173 3229
Size: 54 1425 50 275 18 177 166 1056
Cuts at: 3229 4118 5747 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1056 889 275 177 166 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2412 2517 3712 1353
Size: 723 336 105 1195 3423
Fragments arranged by size:
3423 1195 723 336 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2863 2863
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2906 2954 2999 3005 3845 4442 5229
Size: 393 1274 48 45 6 840 597 787
Cuts at: 5229 5497 1239
Size: 268 1524
Fragments arranged by size:
1524 1274 840 787 597 393 268 48
45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2406 2261
Size: 145 5637
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2101 3062 4929 1678
Size: 423 961 1867 2531
Fragments arranged by size:
2531 1867 961 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 4423 4455 4497
Size: 32 135 32 707 32 2112 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 2112 707 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 2827 3107 2827
Size: 280 5502
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2727 4896 5080 1305
Size: 1422 2169 184 2007
Fragments arranged by size:
2169 2007 1422 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4099 4258 518
Size: 648 2574 359 159 2042
Fragments arranged by size:
2574 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2300 2446 2797 3019 3198 3875 5023 5268
Size: 694 146 351 222 179 677 1148 245
Cuts at: 5268 1606
Size: 2120
Fragments arranged by size:
2120 1148 694 677 351 245 222 179
146
NaeI GCC'GGC
Cuts at: 2322 2322
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4291
Fragments arranged by size:
4291 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NgoMIV G'CCGG_C
Cuts at: 2320 2320
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PfoI T'CCnGG_A
Cuts at: 2076 2076
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2473 1613
Size: 860 4922
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2796 2844 3134 830
Size: 1966 48 290 3478
Fragments arranged by size:
3478 1966 290 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2446 3019 2446
Size: 573 5209
SacI G_AGCT'C
Cuts at: 2865 2865
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SapI GCTCTTCn'nnn_
Cuts at: 2076 2076
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2792 2840 3130 4265 4946 5137 826
Size: 219 1747 48 290 1135 681 191 1471
Fragments arranged by size:
1747 1471 1135 681 290 219 191 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 2237 1517
Size: 720 5062
StyI C'CwwG_G
Cuts at: 513 1065 2004 2589 3221 513
Size: 552 939 585 632 3074
Fragments arranged by size:
3074 939 632 585 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2939 3041 3809 3826 3979 4164
Size: 1255 579 780 102 768 17 153 185
Cuts at: 4164 4609 325
Size: 445 1498
Fragments arranged by size:
1498 1255 780 768 579 445 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2057 2676 4028
Size: 274 80 33 51 1443 80 619 1352
Cuts at: 4028 5740 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1352 619 274 138 80 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 5742 5774 2126
Size: 32 3584 32 2134
Fragments arranged by size:
3584 2134 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2534 3911 2534
Size: 1377 4405
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI