Restriction map for
pFN21AB9605
(Circular) (Six-base) MAPSORT of: pFN21AB9605.seq Check: 285 from: 1 to: 5914
pFN21AB9605 5914 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3192 3192
Size: 5914
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3257 1937
Size: 1320 4594
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2873 3977 5361 5629 1632
Size: 1241 1104 1384 268 1917
Fragments arranged by size:
1917 1384 1241 1104 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 2089 4643 2089
Size: 2554 3360
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2519 2978 1184
Size: 1335 459 4120
Fragments arranged by size:
4120 1335 459
AlwNI CAG_nnn'CTG
Cuts at: 2249 2252 3657 5229 2249
Size: 3 1405 1572 2934
Fragments arranged by size:
2934 1572 1405 3
ApaI G_GGCC'C
Cuts at: 2962 2962
Size: 5914
ApaLI G'TGCA_C
Cuts at: 2328 3971 5127 2328
Size: 1643 1156 3115
Fragments arranged by size:
3115 1643 1156
ApoI r'AATT_y
Cuts at: 1493 3223 3449 3601 5710 1493
Size: 1730 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 2690 2701 2876 3239 1870
Size: 73 735 12 11 175 363 4545
Fragments arranged by size:
4545 735 363 175 73 12 11
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 2897 2947 3025 3235
Size: 325 217 627 384 726 50 78 210
Cuts at: 3235 4690 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 726 627 384 325 217 210
78 50
BanII G_rGCy'C
Cuts at: 1391 2129 2962 3233 1391
Size: 738 833 271 4072
Fragments arranged by size:
4072 833 738 271
BbeI G_GCGC'C
Cuts at: 2175 2901 2951 2175
Size: 726 50 5138
Fragments arranged by size:
5138 726 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2620 928
Size: 971 721 4222
Fragments arranged by size:
4222 971 721
BbvCI CC'TCA_GC
Cuts at: 2651 2651
Size: 5914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2186 2220 4105 4139 1903
Size: 34 249 34 1885 34 3678
Fragments arranged by size:
3678 1885 249 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2741 2796
Size: 107 122 71 631 605 616 453 55
Cuts at: 2796 3001 3152 3174 4525 136
Size: 205 151 22 1351 1525
Fragments arranged by size:
1525 1351 631 616 605 453 205 151
122 107 71 55 22
BglII A'GATC_T
Cuts at: 5909 5909
Size: 5914
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 2332 2962 3975 5131 1790
Size: 542 630 1013 1156 2573
Fragments arranged by size:
2573 1156 1013 630 542
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3645 4603 442
Size: 416 53 83 399 24 2228 958 1753
Fragments arranged by size:
2228 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2791 4574 1239
Size: 845 707 1783 2579
Fragments arranged by size:
2579 1783 845 707
Bpu10I CC'TnA_GC
Cuts at: 2651 2651
Size: 5914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4039 4904 5202 4039
Size: 865 298 4751
Fragments arranged by size:
4751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4577 882
Size: 617 3078 2219
Fragments arranged by size:
3078 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2172 2898 2948 3721
Size: 53 83 186 936 639 726 50 773
Cuts at: 3721 4103 4738 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 773 726 639 635 382 186
83 53 50
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5689
Fragments arranged by size:
5689 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2708 2792 2927 1186
Size: 1057 465 84 135 4173
Fragments arranged by size:
4173 1057 465 135 84
BseYI C'CCAG_C
Cuts at: 3004 5117 5613 3004
Size: 2113 496 3305
Fragments arranged by size:
3305 2113 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2125 1163
Size: 573 389 4952
Fragments arranged by size:
4952 573 389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2160 3036 3105 3364 4125 5153
Size: 275 186 159 876 69 259 761 1028
Cuts at: 5153 1540
Size: 2301
Fragments arranged by size:
2301 1028 876 761 275 259 186 159
69
BsiHKAI G_wGCw'C
Cuts at: 2332 3975 4060 5131 2332
Size: 1643 85 1071 3115
Fragments arranged by size:
3115 1643 1071 85
BsiWI C'GTAC_G
Cuts at: 2979 2979
Size: 5914
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
BsmBI CGTCTCn'nnnn_
Cuts at: 2106 3194 2106
Size: 1088 4826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2332 2962 3233 3975 4060 5131
Size: 399 339 203 630 271 742 85 1071
Cuts at: 5131 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 630 399 339 271 203
85
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3192 3269 844
Size: 2348 77 3489
Fragments arranged by size:
3489 2348 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2319 2971 3046 3801 1394
Size: 429 496 652 75 755 3507
Fragments arranged by size:
3507 755 652 496 429 75
BsrDI GCAATG_nn'
Cuts at: 65 1248 4409 4583 65
Size: 1183 3161 174 1396
Fragments arranged by size:
3161 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2665 2995 3104 4558 4745 1700
Size: 110 855 330 109 1454 187 2869
Fragments arranged by size:
2869 1454 855 330 187 110 109
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 2661 2782 3009 3011 3111 1925
Size: 736 121 227 2 100 4728
Fragments arranged by size:
4728 736 227 121 100 2
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2249 2252 794
Size: 1455 3 4456
Fragments arranged by size:
4456 1455 3
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2179 3244 3611 3996 4013 5465 5551 5563
Size: 207 1065 367 385 17 1452 86 12
Cuts at: 5563 5909 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1065 385 367 346 207 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2861 2861
Size: 5914
BtgI C'CryG_G
Cuts at: 513 1065 2038 2160 2391 2473 2793 2943 513
Size: 552 973 122 231 82 320 150 3484
Fragments arranged by size:
3484 973 552 320 231 150 122 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 2371 487
Size: 683 56 1136 9 4030
Fragments arranged by size:
4030 1136 683 56 9
BtsI GCAGTG_nn'
Cuts at: 2062 3430 4224 4244 2062
Size: 1368 794 20 3732
Fragments arranged by size:
3732 1368 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2534 4921 809
Size: 507 1218 2387 1802
Fragments arranged by size:
2387 1802 1218 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2157 2394
Size: 54 1425 50 275 18 315 12 237
Cuts at: 2394 3102 3361 4250 5879 8
Size: 708 259 889 1629 43
Fragments arranged by size:
1629 1425 889 708 315 275 259 237
54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2157 3102 3361 1537
Size: 275 345 945 259 4090
Fragments arranged by size:
4090 945 345 275 259
EarI CTCTTCn'nnn_
Cuts at: 1353 3844 1353
Size: 2491 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2172 2282 2447 2621 3155 4501
Size: 1064 138 583 110 165 174 534 1346
Cuts at: 4501 4875 5021 387
Size: 374 146 1280
Fragments arranged by size:
1346 1280 1064 583 534 374 174 165
146 138 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2791 2873 3977 4574 5361 5629
Size: 393 452 707 82 1104 597 787 268
Cuts at: 5629 1239
Size: 1524
Fragments arranged by size:
1524 1104 787 707 597 452 393 268
82
EcoO109I rG'GnC_Cy
Cuts at: 2698 2958 2959 2698
Size: 260 1 5653
Fragments arranged by size:
5653 260 1
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 5914
FspI TGC'GCA
Cuts at: 2837 4420 2837
Size: 1583 4331
HaeII r_GCGC'y
Cuts at: 1678 2175 2422 2650 2901 2951 5061 1678
Size: 497 247 228 251 50 2110 2531
Fragments arranged by size:
2531 2110 497 251 247 228 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 4555 4587 4629
Size: 32 135 32 503 32 2448 32 42
Cuts at: 4629 4661 1373
Size: 32 2626
Fragments arranged by size:
2626 2448 503 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KasI G'GCGC_C
Cuts at: 2171 2897 2947 2171
Size: 726 50 5138
Fragments arranged by size:
5138 726 50
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 5028 5212 1305
Size: 687 3036 184 2007
Fragments arranged by size:
3036 2007 687 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 10
Size: 54 1425 343 564 3528
Fragments arranged by size:
3528 1425 564 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 3872 4231 4390 518
Size: 648 1224 1482 359 159 2042
Fragments arranged by size:
2042 1482 1224 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2162 2249 2258 2475 2795 2917 2945
Size: 492 64 87 9 217 320 122 28
Cuts at: 2945 3330 4007 5155 5400 1606
Size: 385 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 492 385 320 245 217
122 87 64 28 9
NaeI GCC'GGC
Cuts at: 2997 3106 2997
Size: 109 5805
NarI GG'CG_CC
Cuts at: 2172 2898 2948 2172
Size: 726 50 5138
Fragments arranged by size:
5138 726 50
NcoI C'CATG_G
Cuts at: 513 1065 2391 513
Size: 552 1326 4036
Fragments arranged by size:
4036 1326 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NgoMIV G'CCGG_C
Cuts at: 2995 3104 2995
Size: 109 5805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2758 3272 4817 1132
Size: 97 1529 514 1545 2229
Fragments arranged by size:
2229 1545 1529 514 97
PasI CC'CwG_GG
Cuts at: 1348 2122 2565 1348
Size: 774 443 4697
Fragments arranged by size:
4697 774 443
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PspOMI G'GGCC_C
Cuts at: 2958 2958
Size: 5914
PstI C_TGCA'G
Cuts at: 830 2257 2957 3153 3266 830
Size: 1427 700 196 113 3478
Fragments arranged by size:
3478 1427 700 196 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2249 2249
Size: 5914
SacII CC_GC'GG
Cuts at: 2163 2476 2796 2946 2163
Size: 313 320 150 5131
Fragments arranged by size:
5131 320 313 150
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 2957 3153 3266 2957
Size: 196 113 5605
Fragments arranged by size:
5605 196 113
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2253 2953 3149 3262 4397 5078 5269
Size: 219 1208 700 196 113 1135 681 191
Cuts at: 5269 826
Size: 1471
Fragments arranged by size:
1471 1208 1135 700 681 219 196 191
113
SfoI GGC'GCC
Cuts at: 2173 2899 2949 2173
Size: 726 50 5138
Fragments arranged by size:
5138 726 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 2680 2703 2878 3241 1872
Size: 808 23 175 363 4545
Fragments arranged by size:
4545 808 363 175 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 4919 5181 820
Size: 197 926 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 2758 3272 1132
Size: 1626 514 3774
Fragments arranged by size:
3774 1626 514
SrfI GCCC'GGGC
Cuts at: 2680 2680
Size: 5914
SspI AAT'ATT
Cuts at: 5 52 3838 5
Size: 47 3786 2081
Fragments arranged by size:
3786 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 2391 3353 513
Size: 552 1326 962 3074
Fragments arranged by size:
3074 1326 962 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3941 3958 4111 4296 4741 325
Size: 1255 2361 17 153 185 445 1498
Fragments arranged by size:
2361 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 3120 4160
Size: 274 80 33 51 1443 630 513 1040
Cuts at: 4160 5872 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1040 630 513 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 2624 1316
Size: 1308 4606
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 2678 2701 2876 3239 1870
Size: 808 23 175 363 4545
Fragments arranged by size:
4545 808 363 175 23
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BstZ17I
DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SwaI XcmI