Restriction map for
pFN21AB9597
(Circular) (Six-base) MAPSORT of: pFN21AB9597.seq Check: 728 from: 1 to: 6196
pFN21AB9597 6196 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3539 1937
Size: 1602 4594
Acc65I G'GTAC_C
Cuts at: 2938 3517 2938
Size: 579 5617
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4259 5643 5911 1632
Size: 2627 1384 268 1917
Fragments arranged by size:
2627 1917 1384 268
AfeI AGC'GCT
Cuts at: 2669 2669
Size: 6196
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 2631 5095 6072 1203
Size: 1428 2464 977 1327
Fragments arranged by size:
2464 1428 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 2620 4925 2620
Size: 2305 3891
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2669 1184
Size: 1485 4711
AlwNI CAG_nnn'CTG
Cuts at: 2022 2686 3020 3939 5511 2022
Size: 664 334 919 1572 2707
Fragments arranged by size:
2707 1572 919 664 334
ApaI G_GGCC'C
Cuts at: 2139 2334 3044 3050 3056 2139
Size: 195 710 6 6 5279
Fragments arranged by size:
5279 710 195 6 6
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 3505 3731 3883 5992 1493
Size: 2012 226 152 2109 1697
Fragments arranged by size:
2109 2012 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2265 2293 2333 2587 3037 3043
Size: 73 205 117 28 40 254 450 6
Cuts at: 3043 3049 3292 3425 3521 1870
Size: 6 243 133 96 4545
Fragments arranged by size:
4545 450 254 243 205 133 117 96
73 40 28 6 6
BamHI G'GATC_C
Cuts at: 2758 3526 3893 2758
Size: 768 367 5061
Fragments arranged by size:
5061 768 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2795 2938 3140 3218
Size: 325 217 627 516 492 143 202 78
Cuts at: 3218 3296 3517 4972 618
Size: 78 221 1455 1842
Fragments arranged by size:
1842 1455 627 516 492 325 221 217
202 143 78 78
BanII G_rGCy'C
Cuts at: 1391 2051 2121 2139 2250 2294 2334 2375 2435
Size: 660 70 18 111 44 40 41 60
Cuts at: 2435 3044 3050 3056 3311 3515 1391
Size: 609 6 6 255 204 4072
Fragments arranged by size:
4072 660 609 255 204 111 70 60
44 41 40 18 6 6
BbeI G_GCGC'C
Cuts at: 2307 2799 3222 3300 2307
Size: 492 423 78 5203
Fragments arranged by size:
5203 492 423 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2312 2750 3060
Size: 107 122 71 631 605 640 438 310
Cuts at: 3060 3273 3483 4807 136
Size: 213 210 1324 1525
Fragments arranged by size:
1525 1324 640 631 605 438 310 213
210 122 107 71
BglII A'GATC_T
Cuts at: 2460 6191 2460
Size: 3731 2465
BlpI GC'TnA_GC
Cuts at: 3613 3613
Size: 6196
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2334 3044 3050 3056 4257 5413 1790
Size: 349 195 710 6 6 1201 1156 2573
Fragments arranged by size:
2573 1201 1156 710 349 195 6 6
BmgBI CAC'GTC
Cuts at: 1318 2089 2155 1318
Size: 771 66 5359
Fragments arranged by size:
5359 771 66
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3927 4885 442
Size: 416 53 83 399 24 2510 958 1753
Fragments arranged by size:
2510 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4856 1239
Size: 3617 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3167 4321 5186 5484 3167
Size: 1154 865 298 3879
Fragments arranged by size:
3879 1154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4859 882
Size: 617 3360 2219
Fragments arranged by size:
3360 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2261 2304 2659 2796
Size: 53 83 186 936 728 43 355 137
Cuts at: 2796 3116 3219 3297 4003 4385 5020 275
Size: 320 103 78 706 382 635 1451
Fragments arranged by size:
1451 936 728 706 635 382 355 320
186 137 103 83 78 53 43
BsaWI w'CCGG_w
Cuts at: 4629 5027 5301 5448 5859 4629
Size: 398 274 147 411 4966
Fragments arranged by size:
4966 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5971
Fragments arranged by size:
5971 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2234 1186
Size: 1045 3 5148
Fragments arranged by size:
5148 1045 3
BseYI C'CCAG_C
Cuts at: 2122 3013 3088 5399 5895 2122
Size: 891 75 2311 496 2423
Fragments arranged by size:
2423 2311 891 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3225 1163
Size: 573 1489 4134
Fragments arranged by size:
4134 1489 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2280 2482 2628 3646 4407
Size: 275 186 57 222 202 146 1018 761
Cuts at: 4407 5435 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 222 202 186
146 57
BsiHKAI G_wGCw'C
Cuts at: 2051 2121 2160 4257 4342 5413 2051
Size: 70 39 2097 85 1071 2834
Fragments arranged by size:
2834 2097 1071 85 70 39
BsmI GAATG_Cn'
Cuts at: 3707 3800 3707
Size: 93 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2121 2139 2160 2250 2294 2334
Size: 399 261 70 18 21 90 44 40
Cuts at: 2334 2375 2435 3044 3050 3056 3311 3515 4257
Size: 41 60 609 6 6 255 204 742
Cuts at: 4257 4342 5413 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 609 399 261 255 204
90 85 70 60 44 41 40 21
18 6 6
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3551 844
Size: 2707 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2921 4083 1394
Size: 429 1098 1162 3507
Fragments arranged by size:
3507 1162 1098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4691 4865 65
Size: 1183 3443 174 1396
Fragments arranged by size:
3443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2854 3137 3299 3473 4840 5027 1700
Size: 110 1044 283 162 174 1367 187 2869
Fragments arranged by size:
2869 1367 1044 283 187 174 162 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 2171 4256 5268 2171
Size: 2085 1012 3099
Fragments arranged by size:
3099 2085 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2022 2686 794
Size: 1228 664 4304
Fragments arranged by size:
4304 1228 664
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 2460 2565 2758 3526 3893 4278 4295 5747
Size: 488 105 193 768 367 385 17 1452
Cuts at: 5747 5833 5845 6191 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 768 488 385 367 346 193
105 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3031 3445 3031
Size: 414 5782
BtgI C'CryG_G
Cuts at: 513 1065 2164 2181 2307 2317 2415 2745 2904
Size: 552 1099 17 126 10 98 330 159
Cuts at: 2904 3144 3268 3336 513
Size: 240 124 68 3373
Fragments arranged by size:
3373 1099 552 330 240 159 126 124
98 68 17 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2386 487
Size: 683 56 1160 4297
Fragments arranged by size:
4297 1160 683 56
BtsI GCAGTG_nn'
Cuts at: 3712 4506 4526 3712
Size: 794 20 5382
Fragments arranged by size:
5382 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6161
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2558 5203 809
Size: 507 1242 2645 1802
Fragments arranged by size:
2645 1802 1242 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2167 2277
Size: 54 1425 50 275 18 225 112 110
Cuts at: 2277 2418 2479 2625 3366 3403 3643 4532 6161
Size: 141 61 146 741 37 240 889 1629
Cuts at: 6161 8
Size: 43
Fragments arranged by size:
1629 1425 889 741 275 240 225 146
141 112 110 61 54 50 43 37
18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2277 2479 2625 3643 1537
Size: 275 243 222 202 146 1018 4090
Fragments arranged by size:
4090 1018 275 243 222 202 146
EarI CTCTTCn'nnn_
Cuts at: 1353 4126 1353
Size: 2773 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 4783 5157 5303 387
Size: 1064 138 930 2264 374 146 1280
Fragments arranged by size:
2264 1280 1064 930 374 146 138
EcoICRI GAG'CTC
Cuts at: 2049 2119 2049
Size: 70 6126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4259 4856 5643 5911 1239
Size: 393 2627 597 787 268 1524
Fragments arranged by size:
2627 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3030 3030
Size: 6196
EcoO109I rG'GnC_Cy
Cuts at: 2136 2330 3053 3178 3341 3450 2136
Size: 194 723 125 163 109 4882
Fragments arranged by size:
4882 723 194 163 125 109
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
FseI GG_CCGG'CC
Cuts at: 2858 2858
Size: 6196
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 2307 2671 2769 2799 3222 3300 5343 1678
Size: 629 364 98 30 423 78 2043 2531
Fragments arranged by size:
2531 2043 629 423 364 98 78 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2207 2239 2606
Size: 32 135 32 449 32 154 32 367
Cuts at: 2606 2638 4837 4869 4911 4943 1373
Size: 32 2199 32 42 32 2626
Fragments arranged by size:
2626 2199 449 367 154 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3540 3786 669
Size: 988 281 1602 246 3079
Fragments arranged by size:
3079 1602 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6196
HpaI GTT'AAC
Cuts at: 3786 3786
Size: 6196
KasI G'GCGC_C
Cuts at: 2303 2795 3218 3296 2303
Size: 492 423 78 5203
Fragments arranged by size:
5203 492 423 78
KpnI G_GTAC'C
Cuts at: 2942 3521 2942
Size: 579 5617
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3002 5310 5494 1305
Size: 1697 2308 184 2007
Fragments arranged by size:
2308 2007 1697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 3368 3405 10
Size: 54 1425 343 588 948 37 2801
Fragments arranged by size:
2801 1425 948 588 343 54 37
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2414 4154 4513 4672 518
Size: 648 921 327 1740 359 159 2042
Fragments arranged by size:
2042 1740 921 648 359 327 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2106 2183 2285 2309 2319 2327 2377
Size: 413 87 77 102 24 10 8 50
Cuts at: 2377 2478 2747 2906 2932 3146 3338 3612 4289
Size: 101 269 159 26 214 192 274 677
Cuts at: 4289 5437 5682 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 413 274 269 245 214
192 159 102 101 87 77 50 26
24 10 8
NaeI GCC'GGC
Cuts at: 2856 3139 3301 3475 2856
Size: 283 162 174 5577
Fragments arranged by size:
5577 283 174 162
NarI GG'CG_CC
Cuts at: 2304 2796 3219 3297 2304
Size: 492 423 78 5203
Fragments arranged by size:
5203 492 423 78
NcoI C'CATG_G
Cuts at: 513 1065 2415 513
Size: 552 1350 4294
Fragments arranged by size:
4294 1350 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NgoMIV G'CCGG_C
Cuts at: 2854 3137 3299 3473 2854
Size: 283 162 174 5577
Fragments arranged by size:
5577 283 174 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 2277 2479 3643 2277
Size: 202 1164 4830
Fragments arranged by size:
4830 1164 202
NspI r_CATG'y
Cuts at: 1132 1229 2635 3554 5099 1132
Size: 97 1406 919 1545 2229
Fragments arranged by size:
2229 1545 1406 919 97
PasI CC'CwG_GG
Cuts at: 1348 2011 1348
Size: 663 5533
PciI A'CATG_T
Cuts at: 2631 5095 2631
Size: 2464 3732
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3262 3364 1108
Size: 2154 102 3940
Fragments arranged by size:
3940 2154 102
PfoI T'CCnGG_A
Cuts at: 3313 3313
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3341 3450 3341
Size: 109 6087
PshAI GACnn'nnGTC
Cuts at: 1613 2347 1613
Size: 734 5462
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PspOMI G'GGCC_C
Cuts at: 2135 2330 3040 3046 3052 2135
Size: 195 710 6 6 5279
Fragments arranged by size:
5279 710 195 6 6
PstI C_TGCA'G
Cuts at: 830 2430 3548 830
Size: 1600 1118 3478
Fragments arranged by size:
3478 1600 1118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
PvuII CAG'CTG
Cuts at: 2019 2932 2019
Size: 913 5283
SacI G_AGCT'C
Cuts at: 2051 2121 2051
Size: 70 6126
SacII CC_GC'GG
Cuts at: 2184 2310 2320 2748 2907 3147 3339 2184
Size: 126 10 428 159 240 192 5041
Fragments arranged by size:
5041 428 240 192 159 126 10
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SanDI GG'GwC_CC
Cuts at: 3450 3450
Size: 6196
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 2426 3249 3544 4679 5360 5551 826
Size: 219 1381 823 295 1135 681 191 1471
Fragments arranged by size:
1471 1381 1135 823 681 295 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3060 3060
Size: 6196
SfoI GGC'GCC
Cuts at: 2305 2797 3220 3298 2305
Size: 492 423 78 5203
Fragments arranged by size:
5203 492 423 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 3299 1700
Size: 1599 4597
SmaI CCC'GGG
Cuts at: 1872 2150 2267 2295 2335 2589 3045 3051 3294
Size: 278 117 28 40 254 456 6 243
Cuts at: 3294 3427 3523 1872
Size: 133 96 4545
Fragments arranged by size:
4545 456 278 254 243 133 117 96
40 28 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3182 4300 5201 5463 820
Size: 197 926 1239 1118 901 262 1553
Fragments arranged by size:
1553 1239 1118 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SrfI GCCC'GGGC
Cuts at: 2335 3045 3051 2335
Size: 710 6 5480
Fragments arranged by size:
5480 710 6
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 3063 3238 1517
Size: 1546 175 4475
Fragments arranged by size:
4475 1546 175
StyI C'CwwG_G
Cuts at: 513 1065 2415 3635 513
Size: 552 1350 1220 3074
Fragments arranged by size:
3074 1350 1220 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2715 4223 4240 4393 4578 5023 325
Size: 1255 1135 1508 17 153 185 445 1498
Fragments arranged by size:
1508 1498 1255 1135 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4442 6154 96
Size: 274 80 33 51 1443 2465 1712 138
Fragments arranged by size:
2465 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6156 6188 6156
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 2148 2265 2293 2333 2587 3043 3049 3292
Size: 278 117 28 40 254 456 6 243
Cuts at: 3292 3425 3521 1870
Size: 133 96 4545
Fragments arranged by size:
4545 456 278 254 243 133 117 96
40 28 6
XmnI GAAnn'nnTTC
Cuts at: 4325 4325
Size: 6196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SapI
SexAI SwaI XcmI