Restriction map for
pFN21AB9595
(Circular) (Six-base) MAPSORT of: pFN21AB9595.seq Check: 378 from: 1 to: 6364
pFN21AB9595 6364 bp
With 182 enzymes: SgfI *
September 8, 2014 18:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2951 4174 278
Size: 53 83 186 936 1415 1223 2468
Fragments arranged by size:
2468 1415 1223 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3645 3707 1937
Size: 1708 62 4594
Fragments arranged by size:
4594 1708 62
Acc65I G'GTAC_C
Cuts at: 3423 3685 3423
Size: 262 6102
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2911 4427 5811 6079 1632
Size: 1279 1516 1384 268 1917
Fragments arranged by size:
1917 1516 1384 1279 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 2484 5263 6240 1203
Size: 1281 2779 977 1327
Fragments arranged by size:
2779 1327 1281 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2522 1184
Size: 1338 5026
AlwNI CAG_nnn'CTG
Cuts at: 2457 2722 3619 4107 5679 2457
Size: 265 897 488 1572 3142
Fragments arranged by size:
3142 1572 897 488 265
ApaI G_GGCC'C
Cuts at: 2202 2202
Size: 6364
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 3673 3899 4051 6160 1493
Size: 2180 226 152 2109 1697
Fragments arranged by size:
2180 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3246 3246
Size: 6364
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 2173 2824 3124 3251 3496 3689 1870
Size: 73 230 651 300 127 245 193 4545
Fragments arranged by size:
4545 651 300 245 230 193 127 73
BamHI G'GATC_C
Cuts at: 3694 4061 3694
Size: 367 5997
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2419 2764 3056 3170
Size: 325 217 627 263 369 345 292 114
Cuts at: 3170 3423 3685 5140 618
Size: 253 262 1455 1842
Fragments arranged by size:
1842 1455 627 369 345 325 292 263
262 253 217 114
BanII G_rGCy'C
Cuts at: 1391 2202 2783 2988 3080 3683 1391
Size: 811 581 205 92 603 4072
Fragments arranged by size:
4072 811 603 581 205 92
BbeI G_GCGC'C
Cuts at: 3060 3174 3060
Size: 114 6250
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4555 4589 1903
Size: 34 50 34 2534 34 3678
Fragments arranged by size:
3678 2534 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4256 5466 4256
Size: 1210 5154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2099 2126
Size: 107 122 71 631 605 337 90 27
Cuts at: 2126 2826 3011 4975 136
Size: 700 185 1964 1525
Fragments arranged by size:
1964 1525 700 631 605 337 185 122
107 90 71 27
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 2852 2987 3781 2852
Size: 135 794 5435
Fragments arranged by size:
5435 794 135
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2202 2705 2769 4425 5581 1790
Size: 265 147 503 64 1656 1156 2573
Fragments arranged by size:
2573 1656 1156 503 265 147 64
BmgBI CAC'GTC
Cuts at: 1318 3630 1318
Size: 2312 4052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4095 5053 442
Size: 416 53 83 399 24 2678 958 1753
Fragments arranged by size:
2678 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3094 3552 5024 1239
Size: 1855 458 1472 2579
Fragments arranged by size:
2579 1855 1472 458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2561 4489 5354 5652 2561
Size: 1928 865 298 3273
Fragments arranged by size:
3273 1928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3438 5027 882
Size: 617 1939 1589 2219
Fragments arranged by size:
2219 1939 1589 617
BsaAI yAC'GTr
Cuts at: 493 1204 2700 4155 493
Size: 711 1496 1455 2702
Fragments arranged by size:
2702 1496 1455 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2948 3057 3171 4171
Size: 53 83 186 936 1415 109 114 1000
Cuts at: 4171 4553 5188 275
Size: 382 635 1451
Fragments arranged by size:
1451 1415 1000 936 635 382 186 114
109 83 53
BsaWI w'CCGG_w
Cuts at: 3307 3632 4797 5195 5469 5616 6027 3307
Size: 325 1165 398 274 147 411 3644
Fragments arranged by size:
3644 1165 411 398 325 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2459 2489 3627 3657 1178
Size: 30 165 30 1056 30 1138 30 3885
Fragments arranged by size:
3885 1138 1056 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 1186
Size: 1384 4980
BseYI C'CCAG_C
Cuts at: 2715 5567 6063 2715
Size: 2852 496 3016
Fragments arranged by size:
3016 2852 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 1163
Size: 573 819 4972
Fragments arranged by size:
4972 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2103 2599 2733 2849 2979
Size: 275 186 93 9 496 134 116 130
Cuts at: 2979 3015 3219 3273 3486 3814 4575 5603 1540
Size: 36 204 54 213 328 761 1028 2301
Fragments arranged by size:
2301 1028 761 496 328 275 213 204
186 134 130 116 93 54 36 9
BsiHKAI G_wGCw'C
Cuts at: 2988 3080 4425 4510 5581 2988
Size: 92 1345 85 1071 3771
Fragments arranged by size:
3771 1345 1071 92 85
BsmI GAATG_Cn'
Cuts at: 3875 3968 3875
Size: 93 6271
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2202 2705 2769 2783 2988 3080
Size: 399 265 147 503 64 14 205 92
Cuts at: 3080 3683 4425 4510 5581 1391
Size: 603 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 503 399 265 205
147 92 85 64 14
BspEI T'CCGG_A
Cuts at: 3632 6027 3632
Size: 2395 3969
BspHI T'CATG_A
Cuts at: 2943 4253 5983 2943
Size: 1310 1730 3324
Fragments arranged by size:
3324 1730 1310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3201 3719 844
Size: 2357 518 3489
Fragments arranged by size:
3489 2357 518
BsrBI CCG'CTC
Cuts at: 1394 1823 2851 4251 1394
Size: 429 1028 1400 3507
Fragments arranged by size:
3507 1400 1028 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 3218 3380 5008 5195 1700
Size: 110 315 1093 162 1628 187 2869
Fragments arranged by size:
2869 1628 1093 315 187 162 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 2012 3025 3246 1925
Size: 87 1013 221 5043
Fragments arranged by size:
5043 1013 221 87
BssSI C'ACGA_G
Cuts at: 3418 4424 5436 3418
Size: 1006 1012 4346
Fragments arranged by size:
4346 1012 1006
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 3310 1184
Size: 2126 4238
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2230 2041
Size: 189 6175
BstYI r'GATC_y
Cuts at: 1972 3694 4061 4446 4463 5915 6001 6013 6359
Size: 1722 367 385 17 1452 86 12 346
Cuts at: 6359 1972
Size: 1977
Fragments arranged by size:
1977 1722 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2094 2268 2760 2979 3153 3273 513
Size: 552 1029 174 492 219 174 120 3604
Fragments arranged by size:
3604 1029 552 492 219 174 174 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2239 487
Size: 683 56 1013 4612
Fragments arranged by size:
4612 1013 683 56
BtsI GCAGTG_nn'
Cuts at: 3184 3880 4674 4694 3184
Size: 696 794 20 4854
Fragments arranged by size:
4854 794 696 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DraIII CAC_nnn'GTG
Cuts at: 3420 3523 3420
Size: 103 6261
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5371 809
Size: 507 4055 1802
Fragments arranged by size:
4055 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2100 2271
Size: 54 1425 50 275 18 261 9 171
Cuts at: 2271 2596 2730 2846 2976 3012 3070 3216 3270
Size: 325 134 116 130 36 58 146 54
Cuts at: 3270 3382 3483 3811 4700 6329 8
Size: 112 101 328 889 1629 43
Fragments arranged by size:
1629 1425 889 328 325 275 261 171
146 134 130 116 112 101 58 54
54 50 43 36 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2091 2100 2596 2730 2846 2976 3012
Size: 275 279 9 496 134 116 130 36
Cuts at: 3012 3216 3270 3483 3811 1537
Size: 204 54 213 328 4090
Fragments arranged by size:
4090 496 328 279 275 213 204 134
130 116 54 36 9
EarI CTCTTCn'nnn_
Cuts at: 1353 4294 1353
Size: 2941 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2372 2887 3456 4951 5325 5471
Size: 1064 138 783 515 569 1495 374 146
Cuts at: 5471 387
Size: 1280
Fragments arranged by size:
1495 1280 1064 783 569 515 374 146
138
EcoICRI GAG'CTC
Cuts at: 2986 3078 2986
Size: 92 6272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2911 3094 3552 4427 5024 5811 6079
Size: 393 1279 183 458 875 597 787 268
Cuts at: 6079 1239
Size: 1524
Fragments arranged by size:
1524 1279 875 787 597 458 393 268
183
EcoNI CCTnn'n_nnAGG
Cuts at: 3431 3431
Size: 6364
EcoO109I rG'GnC_Cy
Cuts at: 2199 3177 2199
Size: 978 5386
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
FseI GG_CCGG'CC
Cuts at: 3384 3384
Size: 6364
FspI TGC'GCA
Cuts at: 2157 2669 4870 2157
Size: 512 2201 3651
Fragments arranged by size:
3651 2201 512
FspAI rTGC'GCAy
Cuts at: 2669 2669
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 2524 2757 3053 3060 3174 5511 1678
Size: 846 233 296 7 114 2337 2531
Fragments arranged by size:
2531 2337 846 296 233 114 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 3627 3659 5005
Size: 32 135 32 887 32 1136 32 1346
Cuts at: 5005 5037 5079 5111 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1346 1136 887 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 3954 669
Size: 988 281 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 3056 3170 3056
Size: 114 6250
KpnI G_GTAC'C
Cuts at: 3427 3689 3427
Size: 262 6102
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5478 5662 1305
Size: 4173 184 2007
Fragments arranged by size:
4173 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 10
Size: 54 1425 343 441 4101
Fragments arranged by size:
4101 1425 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2138 2267 4322 4681 4840 518
Size: 648 954 18 129 2055 359 159 2042
Fragments arranged by size:
2055 2042 954 648 359 159 129 18
MspA1I CmG'CkG
Cuts at: 1606 2096 2748 2762 2811 2981 3046 3155 3780
Size: 490 652 14 49 170 65 109 625
Cuts at: 3780 4457 5605 5850 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 625 490 245 170
109 65 49 14
NaeI GCC'GGC
Cuts at: 2127 3220 3382 2127
Size: 1093 162 5109
Fragments arranged by size:
5109 1093 162
NarI GG'CG_CC
Cuts at: 3057 3171 3057
Size: 114 6250
NcoI C'CATG_G
Cuts at: 513 1065 2268 513
Size: 552 1203 4609
Fragments arranged by size:
4609 1203 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NgoMIV G'CCGG_C
Cuts at: 2125 3218 3380 2125
Size: 1093 162 5109
Fragments arranged by size:
5109 1093 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 2091 2846 2976 3012 3483 3811 2091
Size: 755 130 36 471 328 4644
Fragments arranged by size:
4644 755 471 328 130 36
NspI r_CATG'y
Cuts at: 1132 1229 2488 3722 5267 1132
Size: 97 1259 1234 1545 2229
Fragments arranged by size:
2229 1545 1259 1234 97
PasI CC'CwG_GG
Cuts at: 1348 2041 2740 1348
Size: 693 699 4972
Fragments arranged by size:
4972 699 693
PciI A'CATG_T
Cuts at: 2484 5263 2484
Size: 2779 3585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6364
PfoI T'CCnGG_A
Cuts at: 2673 2673
Size: 6364
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 4433 4465 5946 5978 3503
Size: 32 898 32 1481 32 3889
Fragments arranged by size:
3889 1481 898 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3177 3177
Size: 6364
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2198 2198
Size: 6364
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2300 2332 2300
Size: 32 6332
PstI C_TGCA'G
Cuts at: 830 3101 3716 830
Size: 2271 615 3478
Fragments arranged by size:
3478 2271 615
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
RsrII CG'GwC_CG
Cuts at: 2648 2648
Size: 6364
SacI G_AGCT'C
Cuts at: 2988 3080 2988
Size: 92 6272
SacII CC_GC'GG
Cuts at: 2097 2763 2982 3156 2097
Size: 666 219 174 5305
Fragments arranged by size:
5305 666 219 174
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 2245 3085 3097 3652 3712 4847 5528
Size: 219 1200 840 12 555 60 1135 681
Cuts at: 5528 5719 826
Size: 191 1471
Fragments arranged by size:
1471 1200 1135 840 681 555 219 191
60 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2099 3011 2099
Size: 912 5452
SfoI GGC'GCC
Cuts at: 3058 3172 3058
Size: 114 6250
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 2175 2826 3253 3498 3691 1872
Size: 303 651 427 245 193 4545
Fragments arranged by size:
4545 651 427 303 245 193
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 4468 5369 5631 820
Size: 197 926 633 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 633 262 197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3722 1132
Size: 2590 3774
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6364
StyI C'CwwG_G
Cuts at: 513 1065 2223 2268 3403 3803 513
Size: 552 1158 45 1135 400 3074
Fragments arranged by size:
3074 1158 1135 552 400 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 4391 4408 4561 4746 5191 325
Size: 1255 1122 1689 17 153 185 445 1498
Fragments arranged by size:
1689 1498 1255 1122 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 4610 6322
Size: 274 80 33 51 1443 165 2468 1712
Cuts at: 6322 96
Size: 138
Fragments arranged by size:
2468 1712 1443 274 165 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6324 6356 6324
Size: 32 6332
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6364
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 2173 2824 3251 3496 3689 1870
Size: 303 651 427 245 193 4545
Fragments arranged by size:
4545 651 427 303 245 193
XmnI GAAnn'nnTTC
Cuts at: 4493 4493
Size: 6364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2949 4172 276
Size: 53 83 186 936 1415 1223 2468
Fragments arranged by size:
2468 1415 1223 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI NruI NsiI
PacI PmlI PspXI PvuII SanDI SapI SexAI SrfI
SwaI XcmI