Restriction map for
pFN21AB9594
(Circular) (Six-base) MAPSORT of: pFN21AB9594.seq Check: 4696 from: 1 to: 5680
pFN21AB9594 5680 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2451 2451
Size: 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AfeI AGC'GCT
Cuts at: 2171 2327 2171
Size: 156 5524
AflII C'TTAA_G
Cuts at: 820 1017 2339 820
Size: 197 1322 4161
Fragments arranged by size:
4161 1322 197
AflIII A'CryG_T
Cuts at: 1203 2025 2289 2653 2655 4579 5556 1203
Size: 822 264 364 2 1924 977 1327
Fragments arranged by size:
1924 1327 977 822 364 264 2
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2327 2459 1184
Size: 1143 132 4405
Fragments arranged by size:
4405 1143 132
AlwNI CAG_nnn'CTG
Cuts at: 2262 2389 3423 4995 2262
Size: 127 1034 1572 2947
Fragments arranged by size:
2947 1572 1034 127
ApaLI G'TGCA_C
Cuts at: 2975 3737 4893 2975
Size: 762 1156 3762
Fragments arranged by size:
3762 1156 762
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2431 2494 3005 1870
Size: 73 150 338 63 511 4545
Fragments arranged by size:
4545 511 338 150 73 63
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 2356 2584 2639 3001
Size: 325 217 627 529 40 228 55 362
Cuts at: 3001 4456 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 529 362 325 228 217
55 40
BanII G_rGCy'C
Cuts at: 1391 2090 2999 1391
Size: 699 909 4072
Fragments arranged by size:
4072 909 699
BbeI G_GCGC'C
Cuts at: 2320 2360 2320
Size: 40 5640
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3871 3905 1903
Size: 34 50 34 1850 34 3678
Fragments arranged by size:
3678 1850 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2378 2557 4291
Size: 107 122 71 631 605 706 179 1734
Cuts at: 4291 136
Size: 1525
Fragments arranged by size:
1734 1525 706 631 605 179 122 107
71
BglII A'GATC_T
Cuts at: 2118 5675 2118
Size: 3557 2123
BlpI GC'TnA_GC
Cuts at: 2111 2414 2876 3097 2111
Size: 303 462 221 4694
Fragments arranged by size:
4694 462 303 221
Bme1580I G_kGCm'C
Cuts at: 1790 2438 2589 2642 2790 2817 2979 3741 4897
Size: 648 151 53 148 27 162 762 1156
Cuts at: 4897 1790
Size: 2573
Fragments arranged by size:
2573 1156 762 648 162 151 148 53
27
BmgBI CAC'GTC
Cuts at: 1318 2886 1318
Size: 1568 4112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2028 2656 3471 493
Size: 711 824 628 815 2702
Fragments arranged by size:
2702 824 815 711 628
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2317 2357 3487 3869
Size: 53 83 186 936 784 40 1130 382
Cuts at: 3869 4504 275
Size: 635 1451
Fragments arranged by size:
1451 1130 936 784 635 382 186 83
53 40
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 1178
Size: 30 165 30 780 30 4645
Fragments arranged by size:
4645 780 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5680
BseYI C'CCAG_C
Cuts at: 2360 2635 2927 4883 5379 2360
Size: 275 292 1956 496 2661
Fragments arranged by size:
2661 1956 496 292 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 1163
Size: 573 519 4588
Fragments arranged by size:
4588 573 519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2020 2237 2808 3130 3891 4919
Size: 275 186 19 217 571 322 761 1028
Cuts at: 4919 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 571 322 275 217 186
19
BsiHKAI G_wGCw'C
Cuts at: 2090 2979 3741 3826 4897 2090
Size: 889 762 85 1071 2873
Fragments arranged by size:
2873 1071 889 762 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2438 2589 2642 2790 2817 2979
Size: 399 300 348 151 53 148 27 162
Cuts at: 2979 2999 3741 3826 4897 1391
Size: 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 348 300 162 151
148 85 53 27 20
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 2451 3035 844
Size: 1553 54 584 3489
Fragments arranged by size:
3489 1553 584 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2602 3567 1394
Size: 429 779 965 3507
Fragments arranged by size:
3507 965 779 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2581 2642 2669 2803 4324 4511 1700
Size: 110 771 61 27 134 1521 187 2869
Fragments arranged by size:
2869 1521 771 187 134 110 61 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 2418 1925
Size: 493 5187
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 2584 794
Size: 1595 195 3890
Fragments arranged by size:
3890 1595 195
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 2701
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2118 2223 3010 3377 3762 3779 5231 5317
Size: 146 105 787 367 385 17 1452 86
Cuts at: 5317 5329 5675 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 787 385 367 346 146 105
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2228 2622 2700 513
Size: 552 1163 394 78 3493
Fragments arranged by size:
3493 1163 552 394 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2515 487
Size: 683 56 1289 3652
Fragments arranged by size:
3652 1289 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DraIII CAC_nnn'GTG
Cuts at: 2968 2968
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2076 2234
Size: 54 1425 50 275 18 187 59 158
Cuts at: 2234 2648 2685 2805 3127 4016 5645 8
Size: 414 37 120 322 889 1629 43
Fragments arranged by size:
1629 1425 889 414 322 275 187 158
120 59 54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2017 2234 2805 3127 1537
Size: 275 205 217 571 322 4090
Fragments arranged by size:
4090 571 322 275 217 205
EarI CTCTTCn'nnn_
Cuts at: 1353 3610 1353
Size: 2257 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2560 2656 2944 4267 4641 4787
Size: 1064 138 971 96 288 1323 374 146
Cuts at: 4787 387
Size: 1280
Fragments arranged by size:
1323 1280 1064 971 374 288 146 138
96
EcoICRI GAG'CTC
Cuts at: 2088 2088
Size: 5680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3743 4340 5127 5395 1239
Size: 393 2111 597 787 268 1524
Fragments arranged by size:
2111 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2152 2152
Size: 5680
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2527 2275
Size: 252 5428
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2173 2270 2320 2329 2360 2724 2840 4827
Size: 495 97 50 9 31 364 116 1987
Cuts at: 4827 1678
Size: 2531
Fragments arranged by size:
2531 1987 495 364 116 97 50 31
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4321 4353 4395 4427 1373
Size: 32 135 32 2749 32 42 32 2626
Fragments arranged by size:
2749 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2616 3024 3270 669
Size: 988 281 678 408 246 3079
Fragments arranged by size:
3079 988 678 408 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KasI G'GCGC_C
Cuts at: 2316 2356 2316
Size: 40 5640
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2370 4794 4978 1305
Size: 1065 2424 184 2007
Fragments arranged by size:
2424 2007 1065 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 2650 10
Size: 54 1425 343 246 572 3040
Fragments arranged by size:
3040 1425 572 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2658 2699 2783 3638 3997 4156 518
Size: 648 1492 41 84 855 359 159 2042
Fragments arranged by size:
2042 1492 855 648 359 159 84 41
MspA1I CmG'CkG
Cuts at: 1606 2110 2230 2591 2746 3096 3773 4921 5166
Size: 504 120 361 155 350 677 1148 245
Cuts at: 5166 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 504 361 350 245 155
120
NaeI GCC'GGC
Cuts at: 2671 2805 2671
Size: 134 5546
NarI GG'CG_CC
Cuts at: 2317 2357 2317
Size: 40 5640
NcoI C'CATG_G
Cuts at: 513 1065 2622 2700 513
Size: 552 1557 78 3493
Fragments arranged by size:
3493 1557 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NgoMIV G'CCGG_C
Cuts at: 2669 2803 2669
Size: 134 5546
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2293 3038 4583 1132
Size: 97 1064 745 1545 2229
Fragments arranged by size:
2229 1545 1064 745 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 2289 4579 2289
Size: 2290 3390
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PmlI CAC'GTG
Cuts at: 2656 2656
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2394 3032 830
Size: 1564 638 3478
Fragments arranged by size:
3478 1564 638
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SacI G_AGCT'C
Cuts at: 2090 2090
Size: 5680
SacII CC_GC'GG
Cuts at: 2231 2231
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2152 2390 2716 3028 4163 4844 5035
Size: 219 1107 238 326 312 1135 681 191
Cuts at: 5035 826
Size: 1471
Fragments arranged by size:
1471 1135 1107 681 326 312 238 219
191
SfoI GGC'GCC
Cuts at: 2318 2358 2318
Size: 40 5640
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 3784 4685 4947 820
Size: 197 926 396 1445 901 262 1553
Fragments arranged by size:
1553 1445 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2622 2700 3119 513
Size: 552 1557 78 419 3074
Fragments arranged by size:
3074 1557 552 419 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2289 3707 3724 3877 4062 4507 325
Size: 1255 709 1418 17 153 185 445 1498
Fragments arranged by size:
1498 1418 1255 709 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 5640 5672 2183
Size: 32 3425 32 2191
Fragments arranged by size:
3425 2191 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FseI
FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI
SwaI XcmI