Restriction map for
pFN21AB9593
(Circular) (Six-base) MAPSORT of: pFN21AB9593.seq Check: 2923 from: 1 to: 5695
pFN21AB9593 5695 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2897 2897
Size: 5695
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3758 5142 5410 1632
Size: 2126 1384 268 1917
Fragments arranged by size:
2126 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 2304 4594 5571 1203
Size: 1101 2290 977 1327
Fragments arranged by size:
2290 1327 1101 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2376 1184
Size: 1192 4503
AlwNI CAG_nnn'CTG
Cuts at: 2020 2277 2363 2684 2767 3438 5010 2020
Size: 257 86 321 83 671 1572 2705
Fragments arranged by size:
2705 1572 671 321 257 86 83
ApaI G_GGCC'C
Cuts at: 2381 2866 2381
Size: 485 5210
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 2770 3004 3230 3382 5491 1493
Size: 1277 234 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 234 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2373 3020 1870
Size: 73 81 349 647 4545
Fragments arranged by size:
4545 647 349 81 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2239 2369 3016 4471
Size: 325 217 627 246 206 130 647 1455
Cuts at: 4471 618
Size: 1842
Fragments arranged by size:
1842 1455 647 627 325 246 217 206
130
BanII G_rGCy'C
Cuts at: 1391 2105 2381 2580 2622 2866 3014 1391
Size: 714 276 199 42 244 148 4072
Fragments arranged by size:
4072 714 276 244 199 148 42
BbeI G_GCGC'C
Cuts at: 2037 2037
Size: 5695
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BbvCI CC'TCA_GC
Cuts at: 2364 2815 2364
Size: 451 5244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3886 3920 1903
Size: 34 50 34 1865 34 3678
Fragments arranged by size:
3678 1865 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2188 4306 136
Size: 107 122 71 631 605 516 2118 1525
Fragments arranged by size:
2118 1525 631 605 516 122 107 71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2244 2372 2381 2540 2866 3756 4912 1790
Size: 454 128 9 159 326 890 1156 2573
Fragments arranged by size:
2573 1156 890 454 326 159 128 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2832 4355 1239
Size: 1593 1523 2579
Fragments arranged by size:
2579 1593 1523
Bpu10I CC'TnA_GC
Cuts at: 2364 2495 2815 2364
Size: 131 320 5244
Fragments arranged by size:
5244 320 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 3820 4685 4983 2819
Size: 1001 865 298 3531
Fragments arranged by size:
3531 1001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2972 3502 3884
Size: 53 83 186 936 501 938 530 382
Cuts at: 3884 4519 275
Size: 635 1451
Fragments arranged by size:
1451 938 936 635 530 501 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2967 4128 4526 4800 4947 5358 2967
Size: 1161 398 274 147 411 3304
Fragments arranged by size:
3304 1161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2044 2074 1178
Size: 30 165 30 641 30 4799
Fragments arranged by size:
4799 641 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 2539 2650 4898 5394 2539
Size: 111 2248 496 2840
Fragments arranged by size:
2840 2248 496 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2817 1163
Size: 573 1081 4041
Fragments arranged by size:
4041 1081 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2448 3145 3906 4934 1540
Size: 275 186 447 697 761 1028 2301
Fragments arranged by size:
2301 1028 761 697 447 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2316 2462 2622 2805 3756 3841 4912 2105
Size: 211 146 160 183 951 85 1071 2888
Fragments arranged by size:
2888 1071 951 211 183 160 146 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2244 2316 2372 2381 2462 2540
Size: 399 315 139 72 56 9 81 78
Cuts at: 2540 2580 2622 2805 2866 3014 3756 3841 4912
Size: 40 42 183 61 148 742 85 1071
Cuts at: 4912 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 315 183 148 139
85 81 78 72 61 56 42 40
9
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2897 3050 844
Size: 2053 153 3489
Fragments arranged by size:
3489 2053 153
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 2447 2669 4339 4526 1700
Size: 110 368 269 222 1670 187 2869
Fragments arranged by size:
2869 1670 368 269 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 2457 794
Size: 1226 437 4032
Fragments arranged by size:
4032 1226 437
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2482 3025 3392 3777 3794 5246 5332 5344
Size: 510 543 367 385 17 1452 86 12
Cuts at: 5344 5690 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 543 510 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2405 2405
Size: 5695
BtgI C'CryG_G
Cuts at: 513 1065 2395 2571 2625 513
Size: 552 1330 176 54 3583
Fragments arranged by size:
3583 1330 552 176 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2059 2262 487
Size: 683 56 833 203 3920
Fragments arranged by size:
3920 833 683 203 56
BtsI GCAGTG_nn'
Cuts at: 2462 2867 3211 4005 4025 2462
Size: 405 344 794 20 4132
Fragments arranged by size:
4132 794 405 344 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2396 2396
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2429 4702 809
Size: 507 1113 2273 1802
Fragments arranged by size:
2273 1802 1113 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2564 2628
Size: 54 1425 50 275 18 261 473 64
Cuts at: 2628 3142 4031 5660 8
Size: 514 889 1629 43
Fragments arranged by size:
1629 1425 889 514 473 275 261 64
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3625 1353
Size: 2272 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2192 4282 4656 4802 387
Size: 1064 138 603 2090 374 146 1280
Fragments arranged by size:
2090 1280 1064 603 374 146 138
EcoICRI GAG'CTC
Cuts at: 2103 2620 2103
Size: 517 5178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2832 3758 4355 5142 5410 1239
Size: 393 1200 926 597 787 268 1524
Fragments arranged by size:
1524 1200 926 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2377 2378 2422 2491 2701 2863 2377
Size: 1 44 69 210 162 5209
Fragments arranged by size:
5209 210 162 69 44 1
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2037 4842 1678
Size: 359 2805 2531
Fragments arranged by size:
2805 2531 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373
Size: 32 135 32 2764 32 42 32 2626
Fragments arranged by size:
2764 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2497 748
Size: 1749 3946
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KasI G'GCGC_C
Cuts at: 2033 2033
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 2630 10
Size: 54 1425 343 261 537 3075
Fragments arranged by size:
3075 1425 537 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3653 4012 4171 518
Size: 648 2487 359 159 2042
Fragments arranged by size:
2487 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 3111 3788 4936 5181 1606
Size: 510 995 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 677 510 245
NaeI GCC'GGC
Cuts at: 2671 2671
Size: 5695
NarI GG'CG_CC
Cuts at: 2034 2034
Size: 5695
NcoI C'CATG_G
Cuts at: 513 1065 2571 513
Size: 552 1506 3637
Fragments arranged by size:
3637 1506 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NgoMIV G'CCGG_C
Cuts at: 2669 2669
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2308 2610 3053 4598 1132
Size: 97 1079 302 443 1545 2229
Fragments arranged by size:
2229 1545 1079 443 302 97
PasI CC'CwG_GG
Cuts at: 1348 2612 2898 1348
Size: 1264 286 4145
Fragments arranged by size:
4145 1264 286
PciI A'CATG_T
Cuts at: 2304 4594 2304
Size: 2290 3405
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2657 1108
Size: 1549 4146
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2422 2422
Size: 5695
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2377 2862 2377
Size: 485 5210
PstI C_TGCA'G
Cuts at: 830 2763 3047 830
Size: 1933 284 3478
Fragments arranged by size:
3478 1933 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SacI G_AGCT'C
Cuts at: 2105 2622 2105
Size: 517 5178
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2065 2759 3043 4178 4859 5050 826
Size: 219 1020 694 284 1135 681 191 1471
Fragments arranged by size:
1471 1135 1020 694 681 284 219 191
SfoI GGC'GCC
Cuts at: 2035 2035
Size: 5695
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 2026 2375 3022 1872
Size: 154 349 647 4545
Fragments arranged by size:
4545 647 349 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2798 3799 4700 4962 820
Size: 197 926 855 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 855 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 2610 3053 1132
Size: 1478 443 3774
Fragments arranged by size:
3774 1478 443
SrfI GCCC'GGGC
Cuts at: 2026 2026
Size: 5695
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2560 2796 1517
Size: 1043 236 4416
Fragments arranged by size:
4416 1043 236
StyI C'CwwG_G
Cuts at: 513 1065 2385 2487 2571 3134 513
Size: 552 1320 102 84 563 3074
Fragments arranged by size:
3074 1320 563 552 102 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 2854 3722 3739 3892 4077 4522
Size: 1255 1004 270 868 17 153 185 445
Cuts at: 4522 325
Size: 1498
Fragments arranged by size:
1498 1255 1004 868 445 270 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3941 5653 96
Size: 274 80 33 51 1443 1964 1712 138
Fragments arranged by size:
1964 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 2024 2373 3020 1870
Size: 154 349 647 4545
Fragments arranged by size:
4545 647 349 154
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SwaI XcmI