Restriction map for
pFN21AB9590
(Circular) (Six-base) MAPSORT of: pFN21AB9590.seq Check: 6713 from: 1 to: 5920
pFN21AB9590 5920 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3730 278
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3263 1937
Size: 1326 4594
Acc65I G'GTAC_C
Cuts at: 3241 3241
Size: 5920
AclI AA'CG_TT
Cuts at: 1563 4047 4420 1563
Size: 2484 373 3063
Fragments arranged by size:
3063 2484 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 3983 5367 5635 1632
Size: 417 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 417 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5723
AflIII A'CryG_T
Cuts at: 1203 4819 5796 1203
Size: 3616 977 1327
Fragments arranged by size:
3616 1327 977
AgeI A'CCGG_T
Cuts at: 4751 4751
Size: 5920
AhdI GACnn_n'nnGTC
Cuts at: 4649 4649
Size: 5920
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 4204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2255
Size: 32 5888
AlwNI CAG_nnn'CTG
Cuts at: 3663 5235 3663
Size: 1572 4348
ApaLI G'TGCA_C
Cuts at: 3977 5133 3977
Size: 1156 4764
ApoI r'AATT_y
Cuts at: 1493 2959 3229 3455 3607 5716 1493
Size: 1466 270 226 152 2109 1697
Fragments arranged by size:
2109 1697 1466 270 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4474 160
Size: 872 3442 1606
Fragments arranged by size:
3442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 3245 1870
Size: 73 171 1131 4545
Fragments arranged by size:
4545 1131 171 73
BamHI G'GATC_C
Cuts at: 3250 3617 3250
Size: 367 5553
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3241 4696 618
Size: 325 217 627 1454 1455 1842
Fragments arranged by size:
1842 1455 1454 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3239 1391
Size: 1848 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2801 2946 928
Size: 971 902 145 3902
Fragments arranged by size:
3902 971 902 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3079 3113 4111 4145 1903
Size: 34 1142 34 998 34 3678
Fragments arranged by size:
3678 1142 998 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3812 5022 3812
Size: 1210 4710
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5920
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4531 136
Size: 107 122 71 631 605 2859 1525
Fragments arranged by size:
2859 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5915 5915
Size: 5920
BlpI GC'TnA_GC
Cuts at: 3337 3337
Size: 5920
Bme1580I G_kGCm'C
Cuts at: 1790 2769 3981 5137 1790
Size: 979 1212 1156 2573
Fragments arranged by size:
2573 1212 1156 979
BmgBI CAC'GTC
Cuts at: 1318 2473 1318
Size: 1155 4765
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3651 4609 442
Size: 416 53 83 399 24 2234 958 1753
Fragments arranged by size:
2234 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2521 2999 3121 4580 1239
Size: 1282 478 122 1459 2579
Fragments arranged by size:
2579 1459 1282 478 122
Bpu10I CC'TnA_GC
Cuts at: 3003 3003
Size: 5920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 4045 4910 5208 2030
Size: 2015 865 298 2742
Fragments arranged by size:
2742 2015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2188 3140 4583 882
Size: 617 689 952 1443 2219
Fragments arranged by size:
2219 1443 952 689 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 2989 3057 3711 493
Size: 711 1560 225 68 654 2702
Fragments arranged by size:
2702 1560 711 654 225 68
BsaBI GATnn'nnATC
Cuts at: 5914 5914
Size: 5920
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3727 4109 4744 275
Size: 53 83 186 936 2194 382 635 1451
Fragments arranged by size:
2194 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3113 4353 4751 5025 5172 5583 3113
Size: 1240 398 274 147 411 3450
Fragments arranged by size:
3450 1240 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3119 3149 1178
Size: 30 165 30 1716 30 3949
Fragments arranged by size:
3949 1716 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2269 2735 3176 3185 3188 1186
Size: 1083 466 441 9 3 3918
Fragments arranged by size:
3918 1083 466 441 9 3
BseYI C'CCAG_C
Cuts at: 2240 3151 5123 5619 2240
Size: 911 1972 496 2541
Fragments arranged by size:
2541 1972 911 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 1163
Size: 573 654 4693
Fragments arranged by size:
4693 654 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2741 3370 4131 5159 1540
Size: 275 186 740 629 761 1028 2301
Fragments arranged by size:
2301 1028 761 740 629 275 186
BsiHKAI G_wGCw'C
Cuts at: 3981 4066 5137 3981
Size: 85 1071 4764
Fragments arranged by size:
4764 1071 85
BsiWI C'GTAC_G
Cuts at: 2985 2985
Size: 5920
BsmI GAATG_Cn'
Cuts at: 2269 3431 3524 2269
Size: 1162 93 4665
Fragments arranged by size:
4665 1162 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2357 2357
Size: 5920
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2769 3239 3981 4066 5137 1391
Size: 399 979 470 742 85 1071 2174
Fragments arranged by size:
2174 1071 979 742 470 399 85
BspEI T'CCGG_A
Cuts at: 5583 5583
Size: 5920
BspHI T'CATG_A
Cuts at: 3809 5539 3809
Size: 1730 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2716 3026 3275 844
Size: 1872 310 249 3489
Fragments arranged by size:
3489 1872 310 249
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 3807 1394
Size: 429 290 1694 3507
Fragments arranged by size:
3507 1694 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 3084 4415 4589 65
Size: 1183 1836 1331 174 1396
Fragments arranged by size:
1836 1396 1331 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4564 4751 1700
Size: 110 2754 187 2869
Fragments arranged by size:
2869 2754 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5920
BssSI C'ACGA_G
Cuts at: 3980 4992 3980
Size: 1012 4908
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2128 794
Size: 1334 4586
BstBI TT'CG_AA
Cuts at: 4724 4724
Size: 5920
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5920
BstXI CCAn_nnnn'nTGG
Cuts at: 2962 2962
Size: 5920
BstYI r'GATC_y
Cuts at: 1972 3119 3250 3617 4002 4019 5471 5557 5569
Size: 1147 131 367 385 17 1452 86 12
Cuts at: 5569 5915 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1147 385 367 346 131 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 513
Size: 552 939 78 4351
Fragments arranged by size:
4351 939 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5181
Fragments arranged by size:
5181 683 56
BtsI GCAGTG_nn'
Cuts at: 3436 4230 4250 3436
Size: 794 20 5106
Fragments arranged by size:
5106 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5885
DraI TTT'AAA
Cuts at: 3224 3224
Size: 5920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4927 809
Size: 507 3611 1802
Fragments arranged by size:
3611 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2520 2738
Size: 54 1425 50 275 18 255 435 218
Cuts at: 2738 2952 3102 3367 4256 5885 8
Size: 214 150 265 889 1629 43
Fragments arranged by size:
1629 1425 889 435 275 265 255 218
214 150 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2738 3367 1537
Size: 275 926 629 4090
Fragments arranged by size:
4090 926 629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2615 3162 3850 1353
Size: 1262 547 688 3423
Fragments arranged by size:
3423 1262 688 547
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4507 4881 5027 387
Size: 1064 138 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2521 2999 3121 3983 4580 5367
Size: 393 417 472 478 122 862 597 787
Cuts at: 5367 5635 1239
Size: 268 1524
Fragments arranged by size:
1524 862 787 597 478 472 417 393
268 122
EcoNI CCTnn'n_nnAGG
Cuts at: 2220 2220
Size: 5920
EcoRI G'AATT_C
Cuts at: 3229 3607 3229
Size: 378 5542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 5920
FspI TGC'GCA
Cuts at: 4426 4426
Size: 5920
HaeII r_GCGC'y
Cuts at: 1678 5067 1678
Size: 3389 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 4561 4593 4635
Size: 32 135 32 1241 32 1716 32 42
Cuts at: 4635 4667 1373
Size: 32 2626
Fragments arranged by size:
2626 1716 1241 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 3264 3510 669
Size: 988 281 220 1106 246 3079
Fragments arranged by size:
3079 1106 988 281 246 220
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5920
HpaI GTT'AAC
Cuts at: 3510 3510
Size: 5920
KpnI G_GTAC'C
Cuts at: 3245 3245
Size: 5920
MfeI C'AATT_G
Cuts at: 3519 3519
Size: 5920
MluI A'CGCG_T
Cuts at: 5796 5796
Size: 5920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2844 5034 5218 1305
Size: 1539 2190 184 2007
Fragments arranged by size:
2190 2007 1539 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 2954 3104 10
Size: 54 1425 343 690 432 150 2826
Fragments arranged by size:
2826 1425 690 432 343 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2762 2882 3878 4237 4396 518
Size: 648 1050 546 120 996 359 159 2042
Fragments arranged by size:
2042 1050 996 648 546 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2737 2743 3336 4013 5161 5406 1606
Size: 1131 6 593 677 1148 245 2120
Fragments arranged by size:
2120 1148 1131 677 593 245 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4429
Fragments arranged by size:
4429 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5920
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5920
NotI GC'GGCC_GC
Cuts at: 2738 3367 2738
Size: 629 5291
NspI r_CATG'y
Cuts at: 1132 1229 3278 4823 1132
Size: 97 2049 1545 2229
Fragments arranged by size:
2229 2049 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5920
PciI A'CATG_T
Cuts at: 4819 4819
Size: 5920
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2173 2401 2716 1108
Size: 1065 228 315 4312
Fragments arranged by size:
4312 1065 315 228
PfoI T'CCnGG_A
Cuts at: 2921 2921
Size: 5920
PmeI GTTT'AAAC
Cuts at: 3224 3224
Size: 5920
PmlI CAC'GTG
Cuts at: 2764 3057 2764
Size: 293 5627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 5502 5534 3989
Size: 32 1481 32 4375
Fragments arranged by size:
4375 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5920
PsiI TTA'TAA
Cuts at: 3490 3490
Size: 5920
PstI C_TGCA'G
Cuts at: 830 2813 3272 830
Size: 1983 459 3478
Fragments arranged by size:
3478 1983 459
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5920
SalI G'TCGA_C
Cuts at: 1936 3262 1936
Size: 1326 4594
SbfI CC_TGCA'GG
Cuts at: 3272 3272
Size: 5920
ScaI AGT'ACT
Cuts at: 4168 5880 4168
Size: 1712 4208
SexAI A'CCwGG_T
Cuts at: 2694 2694
Size: 5920
SfcI C'TryA_G
Cuts at: 826 1045 2672 2809 3268 4403 5084 5275 826
Size: 219 1627 137 459 1135 681 191 1471
Fragments arranged by size:
1627 1471 1135 681 459 219 191 137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5920
SmaI CCC'GGG
Cuts at: 1872 3247 1872
Size: 1375 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 4024 4925 5187 820
Size: 197 926 102 1979 901 262 1553
Fragments arranged by size:
1979 1553 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3711 493
Size: 3218 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5920
SphI G_CATG'C
Cuts at: 1132 3278 1132
Size: 2146 3774
SspI AAT'ATT
Cuts at: 5 52 3844 5
Size: 47 3792 2081
Fragments arranged by size:
3792 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5920
StyI C'CwwG_G
Cuts at: 513 1065 2004 2288 3359 513
Size: 552 939 284 1071 3074
Fragments arranged by size:
3074 1071 939 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 3947 3964 4117 4302 4747 325
Size: 1255 674 1693 17 153 185 445 1498
Fragments arranged by size:
1693 1498 1255 674 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3126 4166 5878
Size: 274 80 33 51 1443 1149 1040 1712
Cuts at: 5878 96
Size: 138
Fragments arranged by size:
1712 1443 1149 1040 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5880 5912 5880
Size: 32 5888
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5920
XbaI T'CTAG_A
Cuts at: 3256 3256
Size: 5920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2080
Size: 5920
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5920
XmaI C'CCGG_G
Cuts at: 1870 3245 1870
Size: 1375 4545
XmnI GAAnn'nnTTC
Cuts at: 4049 4049
Size: 5920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3728 276
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI