Restriction map for
pFN21AB9585
(Circular) (Six-base) MAPSORT of: pFN21AB9585.seq Check: 8292 from: 1 to: 5740
pFN21AB9585 5740 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 3803 5187 5455 1632
Size: 580 1591 1384 268 1917
Fragments arranged by size:
1917 1591 1384 580 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 3049 3275 3427 5536 1493
Size: 1556 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 226 152
AscI GG'CGCG_CC
Cuts at: 2253 2253
Size: 5740
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 3008 3065 1870
Size: 73 222 843 57 4545
Fragments arranged by size:
4545 843 222 73 57
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2269 2433 2578 2650
Size: 325 217 627 359 123 164 145 72
Cuts at: 2650 2731 2767 3061 4516 618
Size: 81 36 294 1455 1842
Fragments arranged by size:
1842 1455 627 359 325 294 217 164
145 123 81 72 36
BanII G_rGCy'C
Cuts at: 1391 2523 2547 2717 2922 2976 3015 3059 1391
Size: 1132 24 170 205 54 39 44 4072
Fragments arranged by size:
4072 1132 205 170 54 44 39 24
BbeI G_GCGC'C
Cuts at: 2150 2273 2437 2582 2735 2771 2150
Size: 123 164 145 153 36 5119
Fragments arranged by size:
5119 164 153 145 123 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2804 928
Size: 971 905 3864
Fragments arranged by size:
3864 971 905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2833 2867 3931 3965 1903
Size: 34 50 34 812 34 1064 34 3678
Fragments arranged by size:
3678 1064 812 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 2703 2765
Size: 107 122 71 631 605 472 559 62
Cuts at: 2765 4351 136
Size: 1586 1525
Fragments arranged by size:
1586 1525 631 605 559 472 122 107
71 62
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 2975 3157 2975
Size: 182 5558
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 2682 1318
Size: 1364 4376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4429 442
Size: 416 53 83 399 24 2054 958 1753
Fragments arranged by size:
2054 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2915 2936 4400 1239
Size: 1022 654 21 1464 2579
Fragments arranged by size:
2579 1464 1022 654 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 3865 4730 5028 2507
Size: 1358 865 298 3219
Fragments arranged by size:
3219 1358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2422 3531 493
Size: 711 1218 1109 2702
Fragments arranged by size:
2702 1218 1109 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2270 2434 2579
Size: 53 83 186 936 614 123 164 145
Cuts at: 2579 2724 2732 2768 3547 3929 4564 275
Size: 145 8 36 779 382 635 1451
Fragments arranged by size:
1451 936 779 635 614 382 186 164
145 145 123 83 53 36 8
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2354 2372 2384 2948
Size: 30 165 30 939 12 18 12 564
Cuts at: 2948 2978 1178
Size: 30 3940
Fragments arranged by size:
3940 939 564 165 30 30 30 18
12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2323 2582 2677 3016 1186
Size: 1137 259 95 339 3910
Fragments arranged by size:
3910 1137 339 259 95
BseYI C'CCAG_C
Cuts at: 2029 2191 2248 4943 5439 2029
Size: 162 57 2695 496 2330
Fragments arranged by size:
2695 2330 496 162 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 2468 1163
Size: 573 708 24 4435
Fragments arranged by size:
4435 708 573 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2409 2742 2928 3190 3951 4979
Size: 275 186 408 333 186 262 761 1028
Cuts at: 4979 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 408 333 275 262 186
186
BsiHKAI G_wGCw'C
Cuts at: 2523 2922 2976 3801 3886 4957 2523
Size: 399 54 825 85 1071 3306
Fragments arranged by size:
3306 1071 825 399 85 54
BsiWI C'GTAC_G
Cuts at: 2121 2616 2121
Size: 495 5245
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
BsmBI CGTCTCn'nnnn_
Cuts at: 2891 2891
Size: 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2547 2717 2922 2976 3015 3059
Size: 399 733 24 170 205 54 39 44
Cuts at: 3059 3801 3886 4957 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 399 205 170 85
54 44 39 24
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2767 3095 844
Size: 1923 328 3489
Fragments arranged by size:
3489 1923 328
BsrBI CCG'CTC
Cuts at: 1394 1823 2658 2938 3627 1394
Size: 429 835 280 689 3507
Fragments arranged by size:
3507 835 689 429 280
BsrDI GCAATG_nn'
Cuts at: 65 1248 4235 4409 65
Size: 1183 2987 174 1396
Fragments arranged by size:
2987 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2183 2404 2764 4384 4571 1700
Size: 110 373 221 360 1620 187 2869
Fragments arranged by size:
2869 1620 373 360 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 2215 2253 2454 2626 1925
Size: 290 38 201 172 5039
Fragments arranged by size:
5039 290 201 172 38
BssSI C'ACGA_G
Cuts at: 2210 3800 4812 2210
Size: 1590 1012 3138
Fragments arranged by size:
3138 1590 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2394 794
Size: 1600 4140
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2080
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2829 3070 3437 3822 3839 5291 5377 5389
Size: 857 241 367 385 17 1452 86 12
Cuts at: 5389 5735 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 857 385 367 346 241 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 2142 2154 2277 2283 2547 2802
Size: 552 1008 69 12 123 6 264 255
Cuts at: 2802 513
Size: 3451
Fragments arranged by size:
3451 1008 552 264 255 123 69 12
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2836 487
Size: 683 56 1610 3391
Fragments arranged by size:
3391 1610 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3256 4050 4070 3034
Size: 222 794 20 4704
Fragments arranged by size:
4704 794 222 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 2068 3044 2068
Size: 976 4764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2157 2280
Size: 54 1425 50 275 18 252 75 123
Cuts at: 2280 2406 2925 3031 3187 4076 5705 8
Size: 126 519 106 156 889 1629 43
Fragments arranged by size:
1629 1425 889 519 275 252 156 126
123 106 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2406 2925 3187 1537
Size: 275 594 519 262 4090
Fragments arranged by size:
4090 594 519 275 262
EarI CTCTTCn'nnn_
Cuts at: 1353 2372 3670 1353
Size: 1019 1298 3423
Fragments arranged by size:
3423 1298 1019
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 2295 2543 4327 4701 4847
Size: 1064 138 528 178 248 1784 374 146
Cuts at: 4847 387
Size: 1280
Fragments arranged by size:
1784 1280 1064 528 374 248 178 146
138
EcoICRI GAG'CTC
Cuts at: 2521 2920 2974 2521
Size: 399 54 5287
Fragments arranged by size:
5287 399 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2261 2915 2936 3803 4400 5187
Size: 393 580 49 654 21 867 597 787
Cuts at: 5187 5455 1239
Size: 268 1524
Fragments arranged by size:
1524 867 787 654 597 580 393 268
49 21
EcoO109I rG'GnC_Cy
Cuts at: 2026 2263 2911 2026
Size: 237 648 4855
Fragments arranged by size:
4855 648 237
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FspI TGC'GCA
Cuts at: 2861 2888 4246 2861
Size: 27 1358 4355
Fragments arranged by size:
4355 1358 27
HaeII r_GCGC'y
Cuts at: 1678 2150 2273 2437 2582 2735 2771 4887 1678
Size: 472 123 164 145 153 36 2116 2531
Fragments arranged by size:
2531 2116 472 164 153 145 123 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2354 2374 2386 2948
Size: 32 135 32 770 12 20 12 562
Cuts at: 2948 2980 4381 4413 4455 4487 1373
Size: 32 1401 32 42 32 2626
Fragments arranged by size:
2626 1401 770 562 135 42 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KasI G'GCGC_C
Cuts at: 2146 2269 2433 2578 2731 2767 2146
Size: 123 164 145 153 36 5119
Fragments arranged by size:
5119 164 153 145 123 36
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 2397 4854 5038 1305
Size: 1080 12 2457 184 2007
Fragments arranged by size:
2457 2007 1080 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 10
Size: 54 1425 343 252 3666
Fragments arranged by size:
3666 1425 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2855 3698 4057 4216 518
Size: 648 912 777 843 359 159 2042
Fragments arranged by size:
2042 912 843 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2144 2156 2248 2549 2667 2720 2962 3004
Size: 538 12 92 301 118 53 242 42
Cuts at: 3004 3156 3833 4981 5226 1606
Size: 152 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 538 301 245 242 152
118 92 53 42 12
NaeI GCC'GGC
Cuts at: 2185 2406 2766 2185
Size: 221 360 5159
Fragments arranged by size:
5159 360 221
NarI GG'CG_CC
Cuts at: 2147 2270 2434 2579 2732 2768 2147
Size: 123 164 145 153 36 5119
Fragments arranged by size:
5119 164 153 145 123 36
NcoI C'CATG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 4180
Fragments arranged by size:
4180 1008 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NgoMIV G'CCGG_C
Cuts at: 2183 2404 2764 2183
Size: 221 360 5159
Fragments arranged by size:
5159 360 221
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 2037 3098 4643 1132
Size: 97 808 1061 1545 2229
Fragments arranged by size:
2229 1545 1061 808 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PfoI T'CCnGG_A
Cuts at: 2913 2913
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2911 2026
Size: 885 4855
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 2946 3092 830
Size: 2116 146 3478
Fragments arranged by size:
3478 2116 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2962 3004 2962
Size: 42 5698
SacI G_AGCT'C
Cuts at: 2523 2922 2976 2523
Size: 399 54 5287
Fragments arranged by size:
5287 399 54
SacII CC_GC'GG
Cuts at: 2145 2157 2550 2145
Size: 12 393 5335
Fragments arranged by size:
5335 393 12
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SanDI GG'GwC_CC
Cuts at: 2026 2026
Size: 5740
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 2942 3088 4223 4904 5095 826
Size: 219 1897 146 1135 681 191 1471
Fragments arranged by size:
1897 1471 1135 681 219 191 146
SfoI GGC'GCC
Cuts at: 2148 2271 2435 2580 2733 2769 2148
Size: 123 164 145 153 36 5119
Fragments arranged by size:
5119 164 153 145 123 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 2183 2764 1700
Size: 483 581 4676
Fragments arranged by size:
4676 581 483
SmaI CCC'GGG
Cuts at: 1872 2167 3067 1872
Size: 295 900 4545
Fragments arranged by size:
4545 900 295
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 3844 4745 5007 820
Size: 197 926 579 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 579 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2073 2448 2727 3179 513
Size: 552 1008 375 279 452 3074
Fragments arranged by size:
3074 1008 552 452 375 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 3767 3784 3937 4122 4567 325
Size: 1255 922 1265 17 153 185 445 1498
Fragments arranged by size:
1498 1265 1255 922 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 3986 5698
Size: 274 80 33 51 1443 846 1163 1712
Cuts at: 5698 96
Size: 138
Fragments arranged by size:
1712 1443 1163 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2800 2011
Size: 789 4951
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 2165 3065 1870
Size: 295 900 4545
Fragments arranged by size:
4545 900 295
XmnI GAAnn'nnTTC
Cuts at: 2993 3869 2993
Size: 876 4864
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SapI SexAI SfiI SrfI SwaI