Restriction map for
pFN21AB9584
(Circular) (Six-base) MAPSORT of: pFN21AB9584.seq Check: 3620 from: 1 to: 5515
pFN21AB9584 5515 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2366 2442 1184
Size: 1182 76 4257
Fragments arranged by size:
4257 1182 76
AlwNI CAG_nnn'CTG
Cuts at: 3258 4830 3258
Size: 1572 3943
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 2254 2512 2551 2840 1870
Size: 73 161 150 258 39 289 4545
Fragments arranged by size:
4545 289 258 161 150 73 39
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2247 2836 4291 618
Size: 325 217 627 264 196 589 1455 1842
Fragments arranged by size:
1842 1455 627 589 325 264 217 196
BanII G_rGCy'C
Cuts at: 1391 2111 2834 1391
Size: 720 723 4072
Fragments arranged by size:
4072 723 720
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4544
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2273 2325 2618
Size: 107 122 71 631 605 601 52 293
Cuts at: 2618 4126 136
Size: 1508 1525
Fragments arranged by size:
1525 1508 631 605 601 293 122 107
71 52
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2250 3576 4732 1790
Size: 460 1326 1156 2573
Fragments arranged by size:
2573 1326 1156 460
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4207
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2287 3246 4204
Size: 416 53 83 399 24 870 959 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 959 958 870 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4175 1239
Size: 2936 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3322 3704 4339 275
Size: 53 83 186 936 1789 382 635 1451
Fragments arranged by size:
1789 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5290
Fragments arranged by size:
5290 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5515
BseYI C'CCAG_C
Cuts at: 2136 2746 2798 4718 5214 2136
Size: 610 52 1920 496 2437
Fragments arranged by size:
2437 1920 610 496 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 1163
Size: 573 312 4630
Fragments arranged by size:
4630 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 3576 3661 4732 2169
Size: 1407 85 1071 2952
Fragments arranged by size:
2952 1407 1071 85
BsmI GAATG_Cn'
Cuts at: 2200 3026 3119 2200
Size: 826 93 4596
Fragments arranged by size:
4596 826 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2169 2250 2834 3576 3661 4732
Size: 399 321 58 81 584 742 85 1071
Cuts at: 4732 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 584 399 321 85 81
58
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2205 3402 1394
Size: 429 382 1197 3507
Fragments arranged by size:
3507 1197 429 382
BsrDI GCAATG_nn'
Cuts at: 65 1248 2643 4010 4184 65
Size: 1183 1395 1367 174 1396
Fragments arranged by size:
1396 1395 1367 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2335 4159 4346 1700
Size: 110 525 1824 187 2869
Fragments arranged by size:
2869 1824 525 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2259 1925
Size: 334 5181
BssSI C'ACGA_G
Cuts at: 2200 3575 4587 2200
Size: 1375 1012 3128
Fragments arranged by size:
3128 1375 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2032 2271 2616 513
Size: 552 939 28 239 345 3412
Fragments arranged by size:
3412 939 552 345 239 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2234 487
Size: 683 56 1008 3768
Fragments arranged by size:
3768 1008 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2274 2313
Size: 54 1425 50 275 18 192 252 39
Cuts at: 2313 2337 2962 3851 5480 8
Size: 24 625 889 1629 43
Fragments arranged by size:
1629 1425 889 625 275 252 192 54
50 43 39 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 1353
Size: 2092 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3578 4175 4962 5230 1239
Size: 393 1946 597 787 268 1524
Fragments arranged by size:
1946 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2427 2427
Size: 5515
EcoO109I rG'GnC_Cy
Cuts at: 2303 2303
Size: 5515
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2041 4662 1678
Size: 363 2621 2531
Fragments arranged by size:
2621 2531 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 4156 4188 4230
Size: 32 135 32 526 32 2026 32 42
Cuts at: 4230 4262 1373
Size: 32 2626
Fragments arranged by size:
2626 2026 526 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 2315 10
Size: 54 1425 343 192 291 3210
Fragments arranged by size:
3210 1425 343 291 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 3473 3832 3991 518
Size: 648 924 1383 359 159 2042
Fragments arranged by size:
2042 1383 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2273 2350 2487 2580 2592 2598 2633 2931
Size: 667 77 137 93 12 6 35 298
Cuts at: 2931 3608 4756 5001 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 667 298 245 137 93
77 35 12 6
NaeI GCC'GGC
Cuts at: 2337 2337
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
NdeI CA'TA_TG
Cuts at: 387 2016 387
Size: 1629 3886
NgoMIV G'CCGG_C
Cuts at: 2335 2335
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2219 2347 2410 2608 2873 4418 1132
Size: 97 990 128 63 198 265 1545 2229
Fragments arranged by size:
2229 1545 990 265 198 128 97 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 2086 2299 1108
Size: 912 66 213 4324
Fragments arranged by size:
4324 912 213 66
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2303 2303
Size: 5515
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2355 2431 2867 830
Size: 1525 76 436 3478
Fragments arranged by size:
3478 1525 436 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2350 2350
Size: 5515
SacII CC_GC'GG
Cuts at: 2274 2274
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SanDI GG'GwC_CC
Cuts at: 2303 2303
Size: 5515
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2351 2427 2863 3998 4679 4870 826
Size: 219 1306 76 436 1135 681 191 1471
Fragments arranged by size:
1471 1306 1135 681 436 219 191 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 2273 2273
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2219 2347 2873 1132
Size: 1087 128 526 3774
Fragments arranged by size:
3774 1087 526 128
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2694 2954 513
Size: 552 939 690 260 3074
Fragments arranged by size:
3074 939 690 552 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 5475 5507 2768
Size: 32 2675 32 2776
Fragments arranged by size:
2776 2675 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SapI SexAI SfoI SrfI SwaI
XcmI