Restriction map for
pFN21AB9577
(Circular) (Six-base) MAPSORT of: pFN21AB9577.seq Check: 4297 from: 1 to: 8338
pFN21AB9577 8338 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3740 4502 5209 3740
Size: 762 707 6869
Fragments arranged by size:
6869 762 707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6148 278
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5681 1937
Size: 3744 4594
Acc65I G'GTAC_C
Cuts at: 5659 5659
Size: 8338
AclI AA'CG_TT
Cuts at: 1563 6465 6838 1563
Size: 4902 373 3063
Fragments arranged by size:
4902 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2819 6401 7785 8053 1632
Size: 1187 3582 1384 268 1917
Fragments arranged by size:
3582 1917 1384 1187 268
AflII C'TTAA_G
Cuts at: 820 1017 3679 820
Size: 197 2662 5479
Fragments arranged by size:
5479 2662 197
AflIII A'CryG_T
Cuts at: 1203 3582 3949 4650 4806 7237 8214 1203
Size: 2379 367 701 156 2431 977 1327
Fragments arranged by size:
2431 2379 1327 977 701 367 156
AgeI A'CCGG_T
Cuts at: 4920 7169 4920
Size: 2249 6089
AhdI GACnn_n'nnGTC
Cuts at: 7067 7067
Size: 8338
AleI CACnn'nnGTG
Cuts at: 1166 2525 3963 4649 5000 1166
Size: 1359 1438 686 351 4504
Fragments arranged by size:
4504 1438 1359 686 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8338
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3939 3971 4907 4939 3939
Size: 32 936 32 7338
Fragments arranged by size:
7338 936 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3200 5134 5551 6081 7653 3200
Size: 1934 417 530 1572 3885
Fragments arranged by size:
3885 1934 1572 530 417
ApaLI G'TGCA_C
Cuts at: 6395 7551 6395
Size: 1156 7182
ApoI r'AATT_y
Cuts at: 1493 3028 3860 3899 4191 5235 5647 5873 6025
Size: 1535 832 39 292 1044 412 226 152
Cuts at: 6025 8134 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1535 1044 832 412 292 226
152 39
AseI AT'TA_AT
Cuts at: 160 1032 3048 3852 6892 160
Size: 872 2016 804 3040 1606
Fragments arranged by size:
3040 2016 1606 872 804
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2355 4976 5663 1870
Size: 73 144 268 2621 687 4545
Fragments arranged by size:
4545 2621 687 268 144 73
AvrII C'CTAG_G
Cuts at: 2060 2060
Size: 8338
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4826 4859 5288 5321 4826
Size: 33 429 33 7843
Fragments arranged by size:
7843 429 33 33
BamHI G'GATC_C
Cuts at: 2490 5668 6035 2490
Size: 3178 367 4793
Fragments arranged by size:
4793 3178 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4153 5596 5659 7114 618
Size: 325 217 627 2366 1443 63 1455 1842
Fragments arranged by size:
2366 1842 1455 1443 627 325 217 63
BanII G_rGCy'C
Cuts at: 1391 2088 2094 2457 3288 5634 5657 1391
Size: 697 6 363 831 2346 23 4072
Fragments arranged by size:
4072 2346 831 697 363 23 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2319 4557 928
Size: 971 420 2238 4709
Fragments arranged by size:
4709 2238 971 420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4967 5001 6529 6563 1903
Size: 34 50 34 2946 34 1528 34 3678
Fragments arranged by size:
3678 2946 1528 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6230 7440 6230
Size: 1210 7128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3211 4954 6949
Size: 107 122 71 631 605 1539 1743 1995
Cuts at: 6949 136
Size: 1525
Fragments arranged by size:
1995 1743 1539 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8333 8333
Size: 8338
BlpI GC'TnA_GC
Cuts at: 2009 4101 5755 2009
Size: 2092 1654 4592
Fragments arranged by size:
4592 2092 1654
Bme1580I G_kGCm'C
Cuts at: 1790 2993 4641 6399 7555 1790
Size: 1203 1648 1758 1156 2573
Fragments arranged by size:
2573 1758 1648 1203 1156
BmgBI CAC'GTC
Cuts at: 1318 2668 3952 1318
Size: 1350 1284 5704
Fragments arranged by size:
5704 1350 1284
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 4226 4760
Size: 416 53 83 399 24 1062 1747 534
Cuts at: 4760 5585 6069 7027 442
Size: 825 484 958 1753
Fragments arranged by size:
1753 1747 1062 958 825 534 484 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3756 3756
Size: 8338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2102 2170 2450 2734 3111 3207 3728
Size: 823 40 68 280 284 377 96 521
Cuts at: 3728 5152 6998 1239
Size: 1424 1846 2579
Fragments arranged by size:
2579 1846 1424 823 521 377 284 280
96 68 40
Bpu10I CC'TnA_GC
Cuts at: 3201 4603 3201
Size: 1402 6936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3448 3644 3759 6463 7328 7626 3448
Size: 196 115 2704 865 298 4160
Fragments arranged by size:
4160 2704 865 298 196 115
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4740 5460 7001 882
Size: 617 3241 720 1541 2219
Fragments arranged by size:
3241 2219 1541 720 617
BsaAI yAC'GTr
Cuts at: 493 1204 2505 3814 4651 6129 493
Size: 711 1301 1309 837 1478 2702
Fragments arranged by size:
2702 1478 1309 1301 837 711
BsaBI GATnn'nnATC
Cuts at: 4583 8332 4583
Size: 3749 4589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2751 4521 5196 6145
Size: 53 83 186 936 1218 1770 675 949
Cuts at: 6145 6527 7162 275
Size: 382 635 1451
Fragments arranged by size:
1770 1451 1218 949 936 675 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2487 4575 4920 5211 6771 7169 7443 7590 8001
Size: 2088 345 291 1560 398 274 147 411
Cuts at: 8001 2487
Size: 2824
Fragments arranged by size:
2824 2088 1560 411 398 345 291 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 2457 2468 2487
Size: 30 165 30 1020 30 4 11 19
Cuts at: 2487 2498 4617 4647 5150 5180 1178
Size: 11 2119 30 503 30 4336
Fragments arranged by size:
4336 2119 1020 503 165 30 30 30
30 30 19 11 11 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3368 3641 4130 4520 5300 1186
Size: 2182 273 489 390 780 4224
Fragments arranged by size:
4224 2182 780 489 390 273
BseYI C'CCAG_C
Cuts at: 2937 4667 5014 5019 5440 5633 7541 8037 2937
Size: 1730 347 5 421 193 1908 496 3238
Fragments arranged by size:
3238 1908 1730 496 421 347 193 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4427 5523 1163
Size: 573 2691 1096 3978
Fragments arranged by size:
3978 2691 1096 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5118 5328 5788 6549 7577 1540
Size: 275 186 3117 210 460 761 1028 2301
Fragments arranged by size:
3117 2301 1028 761 460 275 210 186
BsiHKAI G_wGCw'C
Cuts at: 2094 3288 6399 6484 7555 2094
Size: 1194 3111 85 1071 2877
Fragments arranged by size:
3111 2877 1194 1071 85
BsiWI C'GTAC_G
Cuts at: 5329 5329
Size: 8338
BsmI GAATG_Cn'
Cuts at: 4694 5849 5942 4694
Size: 1155 93 7090
Fragments arranged by size:
7090 1155 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2742 4536 2742
Size: 1794 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2094 2457 2993 3288 4641 5634
Size: 399 298 6 363 536 295 1353 993
Cuts at: 5634 5657 6399 6484 7555 1391
Size: 23 742 85 1071 2174
Fragments arranged by size:
2174 1353 1071 993 742 536 399 363
298 295 85 23 6
BspEI T'CCGG_A
Cuts at: 4575 8001 4575
Size: 3426 4912
BspHI T'CATG_A
Cuts at: 2764 3826 6227 7957 2764
Size: 1062 2401 1730 3145
Fragments arranged by size:
3145 2401 1730 1062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3740 4149 4488 4502 5209 5693 844
Size: 2896 409 339 14 707 484 3489
Fragments arranged by size:
3489 2896 707 484 409 339 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 6225 1394
Size: 429 1846 2556 3507
Fragments arranged by size:
3507 2556 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6833 7007 65
Size: 1183 5585 174 1396
Fragments arranged by size:
5585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4144 4465 4920 6982 7169 1700
Size: 110 2334 321 455 2062 187 2869
Fragments arranged by size:
2869 2334 2062 455 321 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8338
BssHII G'CGCG_C
Cuts at: 1925 3371 1925
Size: 1446 6892
BssSI C'ACGA_G
Cuts at: 4427 4445 4928 6398 7410 4427
Size: 18 483 1470 1012 5355
Fragments arranged by size:
5355 1470 1012 483 18
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5551 794
Size: 4757 3581
BstBI TT'CG_AA
Cuts at: 7142 7142
Size: 8338
BstEII G'GTnAC_C
Cuts at: 1184 5002 1184
Size: 3818 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3387 2029
Size: 1358 6980
BstYI r'GATC_y
Cuts at: 1972 2490 3694 5668 6035 6420 6437 7889 7975
Size: 518 1204 1974 367 385 17 1452 86
Cuts at: 7975 7987 8333 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1974 1452 1204 518 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2329 2871 2994 4473 4788 5059 513
Size: 552 1264 542 123 1479 315 271 3792
Fragments arranged by size:
3792 1479 1264 552 542 315 271 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7599
Fragments arranged by size:
7599 683 56
BtsI GCAGTG_nn'
Cuts at: 2685 4057 5540 5854 6648 6668 2685
Size: 1372 1483 314 794 20 4355
Fragments arranged by size:
4355 1483 1372 794 314 20
ClaI AT'CG_AT
Cuts at: 1554 5362 1554
Size: 3808 4530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4033 4068 529
Size: 35 3469 35 4799
Fragments arranged by size:
4799 3469 35 35
DraI TTT'AAA
Cuts at: 3768 5642 3768
Size: 1874 6464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5195 5332 7345 809
Size: 507 3879 137 2013 1802
Fragments arranged by size:
3879 2013 1802 507 137
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3271 3723 4467
Size: 54 1425 50 275 18 1441 452 744
Cuts at: 4467 4726 5115 5785 6674 8303 8
Size: 259 389 670 889 1629 43
Fragments arranged by size:
1629 1441 1425 889 744 670 452 389
275 259 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5115 5785 1537
Size: 275 3303 670 4090
Fragments arranged by size:
4090 3303 670 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 6268 1353
Size: 1382 3533 3423
Fragments arranged by size:
3533 3423 1382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2066 6925 7299 7445 387
Size: 1064 138 477 4859 374 146 1280
Fragments arranged by size:
4859 1280 1064 477 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 3286 2092
Size: 1194 7144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2102 2170 2450 2734 2819 3111
Size: 393 430 40 68 280 284 85 292
Cuts at: 3111 3207 3728 5152 6401 6998 7785 8053 1239
Size: 96 521 1424 1249 597 787 268 1524
Fragments arranged by size:
1524 1424 1249 787 597 521 430 393
292 284 280 268 96 85 68 40
EcoNI CCTnn'n_nnAGG
Cuts at: 2387 5037 2387
Size: 2650 5688
EcoO109I rG'GnC_Cy
Cuts at: 3712 4138 4933 5131 3712
Size: 426 795 198 6919
Fragments arranged by size:
6919 795 426 198
EcoRI G'AATT_C
Cuts at: 3028 5235 5647 6025 3028
Size: 2207 412 378 5341
Fragments arranged by size:
5341 2207 412 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5059 5059
Size: 8338
FspI TGC'GCA
Cuts at: 6844 6844
Size: 8338
HaeII r_GCGC'y
Cuts at: 1678 2723 7485 1678
Size: 1045 4762 2531
Fragments arranged by size:
4762 2531 1045
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3017 3049 3780 3812 4539
Size: 32 135 32 1445 32 731 32 727
Cuts at: 4539 4571 6979 7011 7053 7085 1373
Size: 32 2408 32 42 32 2626
Fragments arranged by size:
2626 2408 1445 731 727 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 5682 5928 669
Size: 988 281 1479 2265 246 3079
Fragments arranged by size:
3079 2265 1479 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3395 5424 748
Size: 2647 2029 3662
Fragments arranged by size:
3662 2647 2029
HpaI GTT'AAC
Cuts at: 3417 5928 3417
Size: 2511 5827
KpnI G_GTAC'C
Cuts at: 5663 5663
Size: 8338
MfeI C'AATT_G
Cuts at: 5937 5937
Size: 8338
MluI A'CGCG_T
Cuts at: 8214 8214
Size: 8338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2203 3072 4367 4434 4695 5254 5409 7452
Size: 898 869 1295 67 261 559 155 2043
Cuts at: 7452 7636 1305
Size: 184 2007
Fragments arranged by size:
2043 2007 1295 898 869 559 261 184
155 67
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4728 10
Size: 54 1425 343 2896 3620
Fragments arranged by size:
3620 2896 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 2781 3963 4649 5000 6296 6655
Size: 648 1359 256 1182 686 351 1296 359
Cuts at: 6655 6814 518
Size: 159 2042
Fragments arranged by size:
2042 1359 1296 1182 686 648 359 351
256 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2922 3276 5754 6431 7579 7824 1606
Size: 474 842 354 2478 677 1148 245 2120
Fragments arranged by size:
2478 2120 1148 842 677 474 354 245
NaeI GCC'GGC
Cuts at: 4467 4467
Size: 8338
NcoI C'CATG_G
Cuts at: 513 1065 2329 2871 4473 4788 5059 513
Size: 552 1264 542 1602 315 271 3792
Fragments arranged by size:
3792 1602 1264 552 542 315 271
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8338
NgoMIV G'CCGG_C
Cuts at: 4465 4465
Size: 8338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8338
NotI GC'GGCC_GC
Cuts at: 5785 5785
Size: 8338
NspI r_CATG'y
Cuts at: 1132 1229 3586 3794 4596 5696 7241 1132
Size: 97 2357 208 802 1100 1545 2229
Fragments arranged by size:
2357 2229 1545 1100 802 208 97
PasI CC'CwG_GG
Cuts at: 1348 3716 1348
Size: 2368 5970
PciI A'CATG_T
Cuts at: 3582 7237 3582
Size: 3655 4683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2971 1108
Size: 1863 6475
PmeI GTTT'AAAC
Cuts at: 5642 5642
Size: 8338
PmlI CAC'GTG
Cuts at: 2505 4651 2505
Size: 2146 6192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6407 6439 7920 7952 6407
Size: 32 1481 32 6793
Fragments arranged by size:
6793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3712 4138 5131 3712
Size: 426 993 6919
Fragments arranged by size:
6919 993 426
PshAI GACnn'nnGTC
Cuts at: 1613 4287 1613
Size: 2674 5664
PsiI TTA'TAA
Cuts at: 5908 5908
Size: 8338
PspXI vC'TCGA_Gb
Cuts at: 4976 4976
Size: 8338
PstI C_TGCA'G
Cuts at: 830 2150 4168 4499 5547 5690 830
Size: 1320 2018 331 1048 143 3478
Fragments arranged by size:
3478 2018 1320 1048 331 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8338
PvuII CAG'CTG
Cuts at: 2080 2922 2080
Size: 842 7496
SacI G_AGCT'C
Cuts at: 2094 3288 2094
Size: 1194 7144
SalI G'TCGA_C
Cuts at: 1936 5680 1936
Size: 3744 4594
SbfI CC_TGCA'GG
Cuts at: 4499 5690 4499
Size: 1191 7147
ScaI AGT'ACT
Cuts at: 6586 8298 6586
Size: 1712 6626
SfcI C'TryA_G
Cuts at: 826 1045 2146 3432 4164 4460 4495 5543 5686
Size: 219 1101 1286 732 296 35 1048 143
Cuts at: 5686 6821 7502 7693 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1286 1135 1101 1048 732 681 296
219 191 143 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8338
SmaI CCC'GGG
Cuts at: 1872 5665 1872
Size: 3793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3463 3623 3679 3774 4976 6442
Size: 197 926 1520 160 56 95 1202 1466
Cuts at: 6442 7343 7605 820
Size: 901 262 1553
Fragments arranged by size:
1553 1520 1466 1202 926 901 262 197
160 95 56
SnaBI TAC'GTA
Cuts at: 493 6129 493
Size: 5636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8338
SphI G_CATG'C
Cuts at: 1132 5696 1132
Size: 4564 3774
SspI AAT'ATT
Cuts at: 5 52 3046 6262 5
Size: 47 2994 3216 2081
Fragments arranged by size:
3216 2994 2081 47
StuI AGG'CCT
Cuts at: 1517 3736 1517
Size: 2219 6119
StyI C'CwwG_G
Cuts at: 513 1065 2060 2329 2619 2871 4473 4788 5059
Size: 552 995 269 290 252 1602 315 271
Cuts at: 5059 5777 513
Size: 718 3074
Fragments arranged by size:
3074 1602 995 718 552 315 290 271
269 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2461 6365 6382 6535 6720 7165 325
Size: 1255 881 3904 17 153 185 445 1498
Fragments arranged by size:
3904 1498 1255 881 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3780 6584 8296
Size: 274 80 33 51 1443 1803 2804 1712
Cuts at: 8296 96
Size: 138
Fragments arranged by size:
2804 1803 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2217 2249 2423 2455 2457 2489 4261 4293 4320
Size: 32 174 32 2 32 1772 32 27
Cuts at: 4320 4352 5150 5182 8298 8330 2217
Size: 32 798 32 3116 32 2225
Fragments arranged by size:
3116 2225 1772 798 174 32 32 32
32 32 32 32 27 2
Tth111I GACn'n_nGTC
Cuts at: 1316 2558 2669 3930 4523 1316
Size: 1242 111 1261 593 5131
Fragments arranged by size:
5131 1261 1242 593 111
XbaI T'CTAG_A
Cuts at: 5674 5674
Size: 8338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4103 4786 4103
Size: 683 7655
XhoI C'TCGA_G
Cuts at: 1943 4976 1943
Size: 3033 5305
XmaI C'CCGG_G
Cuts at: 1870 5663 1870
Size: 3793 4545
XmnI GAAnn'nnTTC
Cuts at: 5042 6467 5042
Size: 1425 6913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6146 276
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BbvCI BfrBI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspOMI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI