Restriction map for
pFN21AB9574
(Circular) (Six-base) MAPSORT of: pFN21AB9574.seq Check: 8646 from: 1 to: 5845
pFN21AB9574 5845 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2816 3908 5292 5560 1632
Size: 953 231 1092 1384 268 1917
Fragments arranged by size:
1917 1384 1092 953 268 231
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 3015 4744 5721 1203
Size: 1812 1729 977 1327
Fragments arranged by size:
1812 1729 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 2981 1166
Size: 1815 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2479 1184
Size: 1295 4550
AlwNI CAG_nnn'CTG
Cuts at: 2230 3588 5160 2230
Size: 1358 1572 2915
Fragments arranged by size:
2915 1572 1358
ApaI G_GGCC'C
Cuts at: 2949 2949
Size: 5845
ApaLI G'TGCA_C
Cuts at: 2974 3902 5058 2974
Size: 928 1156 3761
Fragments arranged by size:
3761 1156 928
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 3041 3170 1870
Size: 73 71 1027 129 4545
Fragments arranged by size:
4545 1027 129 73 71
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3166 4621 618
Size: 325 217 627 1379 1455 1842
Fragments arranged by size:
1842 1455 1379 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2129 2281 2751 2808 2949 2970 3164 1391
Size: 738 152 470 57 141 21 194 4072
Fragments arranged by size:
4072 738 470 194 152 141 57 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2348 2804 3012 3022 928
Size: 971 449 456 208 10 3751
Fragments arranged by size:
3751 971 456 449 208 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2595 2629 4036 4070 1903
Size: 34 658 34 1407 34 3678
Fragments arranged by size:
3678 1407 658 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4456 136
Size: 107 122 71 631 605 2784 1525
Fragments arranged by size:
2784 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 3119 3262 3119
Size: 143 5702
Bme1580I G_kGCm'C
Cuts at: 1790 2949 2978 3906 5062 1790
Size: 1159 29 928 1156 2573
Fragments arranged by size:
2573 1159 1156 928 29
BmgBI CAC'GTC
Cuts at: 1318 3018 1318
Size: 1700 4145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2685 2838 2920
Size: 416 53 83 399 24 1268 153 82
Cuts at: 2920 3576 4534 442
Size: 656 958 1753
Fragments arranged by size:
1753 1268 958 656 416 399 153 83
82 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3124 3067
Size: 57 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 2155 2308 2388 2984 4505 1239
Size: 862 54 153 80 596 1521 2579
Fragments arranged by size:
2579 1521 862 596 153 80 54
Bpu10I CC'TnA_GC
Cuts at: 2745 2745
Size: 5845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 3652 4034 4669
Size: 53 83 186 936 506 1613 382 635
Cuts at: 4669 275
Size: 1451
Fragments arranged by size:
1613 1451 936 635 506 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2215 2472 2980 4278 4676 4950 5097 5508 2215
Size: 257 508 1298 398 274 147 411 2552
Fragments arranged by size:
2552 1298 508 411 398 274 257 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 2915 2945 2957
Size: 30 165 30 696 30 786 30 12
Cuts at: 2957 2980 2987 3010 1178
Size: 23 7 23 4013
Fragments arranged by size:
4013 786 696 165 30 30 30 30
23 23 12 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2242 2491 2723 2726 1186
Size: 1056 249 232 3 4305
Fragments arranged by size:
4305 1056 249 232 3
BseYI C'CCAG_C
Cuts at: 2312 2827 2999 3059 5048 5544 2312
Size: 515 172 60 1989 496 2613
Fragments arranged by size:
2613 1989 515 496 172 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 2255 2906 2989 3087 1163
Size: 573 401 118 651 83 98 3921
Fragments arranged by size:
3921 651 573 401 118 98 83
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 3295 4056 5084 1540
Size: 275 186 72 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 2978 3906 3991 5062 2978
Size: 928 85 1071 3761
Fragments arranged by size:
3761 1071 928 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
BsmBI CGTCTCn'nnnn_
Cuts at: 2173 2254 2173
Size: 81 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2281 2751 2808 2949 2970 2978
Size: 399 339 152 470 57 141 21 8
Cuts at: 2978 3164 3906 3991 5062 1391
Size: 186 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 470 399 339 186 152
141 85 57 21 8
BspEI T'CCGG_A
Cuts at: 2215 5508 2215
Size: 3293 2552
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2568 2617 3200 844
Size: 1385 339 49 583 3489
Fragments arranged by size:
3489 1385 583 339 49
BsrBI CCG'CTC
Cuts at: 1394 1823 2865 3732 1394
Size: 429 1042 867 3507
Fragments arranged by size:
3507 1042 867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3103 4340 4514 65
Size: 1183 1855 1237 174 1396
Fragments arranged by size:
1855 1396 1237 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4489 4676 1700
Size: 110 2679 187 2869
Fragments arranged by size:
2869 2679 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 2986 1184
Size: 1802 4043
BstXI CCAn_nnnn'nTGG
Cuts at: 2962 3136 2962
Size: 174 5671
BstYI r'GATC_y
Cuts at: 1972 2619 3175 3542 3927 3944 5396 5482 5494
Size: 647 556 367 385 17 1452 86 12
Cuts at: 5494 5840 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 647 556 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2190 2256 2949 513
Size: 552 1125 66 693 3409
Fragments arranged by size:
3409 1125 693 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2250 487
Size: 683 56 1024 4082
Fragments arranged by size:
4082 1024 683 56
BtsI GCAGTG_nn'
Cuts at: 2196 2231 3006 3361 4155 4175 2196
Size: 35 775 355 794 20 3866
Fragments arranged by size:
3866 794 775 355 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2165 4852 809
Size: 507 849 2687 1802
Fragments arranged by size:
2687 1802 849 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2163 2952
Size: 54 1425 50 275 18 240 93 789
Cuts at: 2952 3292 4181 5810 8
Size: 340 889 1629 43
Fragments arranged by size:
1629 1425 889 789 340 275 240 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 3292 1537
Size: 275 258 1222 4090
Fragments arranged by size:
4090 1222 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 2409 2640 3775 1353
Size: 1056 231 1135 3423
Fragments arranged by size:
3423 1135 1056 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2155 2308 2388 2585 2816 2984
Size: 393 469 54 153 80 197 231 168
Cuts at: 2984 3908 4505 5292 5560 1239
Size: 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 469 393 268 231
197 168 153 80 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2564 2687 2564
Size: 123 5722
EcoO109I rG'GnC_Cy
Cuts at: 2680 2892 2945 2680
Size: 212 53 5580
Fragments arranged by size:
5580 212 53
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 4992 1678
Size: 3314 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2069 2099 2101
Size: 32 135 32 455 32 10 30 2
Cuts at: 2101 2131 2516 2548 4486 4518 4560 4592 1373
Size: 30 385 32 1938 32 42 32 2626
Fragments arranged by size:
2626 1938 455 385 135 42 32 32
32 32 32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 3189 3435 669
Size: 988 281 199 1052 246 3079
Fragments arranged by size:
3079 1052 988 281 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4959 5143 1305
Size: 3654 184 2007
Fragments arranged by size:
3654 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2954 10
Size: 54 1425 343 1122 2901
Fragments arranged by size:
2901 1425 1122 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3803 4162 4321 518
Size: 648 1815 822 359 159 2042
Fragments arranged by size:
2042 1815 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2312 3003 3063 3071 3261 3938 5086
Size: 446 260 691 60 8 190 677 1148
Cuts at: 5086 5331 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 691 677 446 260 245 190
60 8
NcoI C'CATG_G
Cuts at: 513 1065 2949 513
Size: 552 1884 3409
Fragments arranged by size:
3409 1884 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 3203 4748 1132
Size: 97 1974 1545 2229
Fragments arranged by size:
2229 1974 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2935 1348
Size: 1587 4258
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2892 2892
Size: 5845
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspOMI G'GGCC_C
Cuts at: 2945 2945
Size: 5845
PstI C_TGCA'G
Cuts at: 830 2057 2226 2458 3197 830
Size: 1227 169 232 739 3478
Fragments arranged by size:
3478 1227 739 232 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 3003 3063 3071 3003
Size: 60 8 5777
Fragments arranged by size:
5777 60 8
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 2053 2222 2454 3193 4328 5009 5200
Size: 219 1008 169 232 739 1135 681 191
Cuts at: 5200 826
Size: 1471
Fragments arranged by size:
1471 1135 1008 739 681 232 219 191
169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 1517
Size: 1200 4645
StyI C'CwwG_G
Cuts at: 513 1065 2949 3284 513
Size: 552 1884 335 3074
Fragments arranged by size:
3074 1884 552 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 2816 3142 3872 3889 4042 4227
Size: 1255 981 255 326 730 17 153 185
Cuts at: 4227 4672 325
Size: 445 1498
Fragments arranged by size:
1498 1255 981 730 445 326 255 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2993 4091 5803
Size: 274 80 33 51 1443 1016 1098 1712
Cuts at: 5803 96
Size: 138
Fragments arranged by size:
1712 1443 1098 1016 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2915 2947 2957 2980 2989 3012 5805
Size: 32 673 32 10 23 9 23 2793
Cuts at: 5805 5837 2210
Size: 32 2218
Fragments arranged by size:
2793 2218 673 32 32 32 23 23
10 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 2758
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI