Restriction map for
pFN21AB9572
(Circular) (Six-base) MAPSORT of: pFN21AB9572.seq Check: 3791 from: 1 to: 7069
pFN21AB9572 7069 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4879 278
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4412 1937
Size: 2475 4594
Acc65I G'GTAC_C
Cuts at: 4390 4390
Size: 7069
AclI AA'CG_TT
Cuts at: 1563 5196 5569 1563
Size: 3633 373 3063
Fragments arranged by size:
3633 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2196 2217 2340 2785 2829 4012 5132 6516
Size: 564 21 123 445 44 1183 1120 1384
Cuts at: 6516 6784 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1183 1120 564 445 268 123
44 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6872
AflIII A'CryG_T
Cuts at: 1203 5968 6945 1203
Size: 4765 977 1327
Fragments arranged by size:
4765 1327 977
AgeI A'CCGG_T
Cuts at: 5900 5900
Size: 7069
AhdI GACnn_n'nnGTC
Cuts at: 5798 5798
Size: 7069
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2567 1184
Size: 1383 5686
AlwNI CAG_nnn'CTG
Cuts at: 2029 2264 2636 3961 3985 4150 4812 6384 2029
Size: 235 372 1325 24 165 662 1572 2714
Fragments arranged by size:
2714 1572 1325 662 372 235 165 24
ApaI G_GGCC'C
Cuts at: 2109 4291 2109
Size: 2182 4887
ApaLI G'TGCA_C
Cuts at: 5126 6282 5126
Size: 1156 5913
ApoI r'AATT_y
Cuts at: 1493 2404 2439 3413 4378 4604 4756 6865 1493
Size: 911 35 974 965 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 965 911 226 152 35
AseI AT'TA_AT
Cuts at: 160 1032 5623 160
Size: 872 4591 1606
Fragments arranged by size:
4591 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2120 2618 3489 4394 1870
Size: 73 165 12 498 871 905 4545
Fragments arranged by size:
4545 905 871 498 165 73 12
BamHI G'GATC_C
Cuts at: 3283 4399 4766 3283
Size: 1116 367 5586
Fragments arranged by size:
5586 1116 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3599 4390 5845 618
Size: 325 217 627 1812 791 1455 1842
Fragments arranged by size:
1842 1812 1455 791 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2109 2951 3245 3728 4291 4388 1391
Size: 718 842 294 483 563 97 4072
Fragments arranged by size:
4072 842 718 563 483 294 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3654 928
Size: 971 1755 4343
Fragments arranged by size:
4343 1755 971
BbvCI CC'TCA_GC
Cuts at: 3746 3746
Size: 7069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5260 5294 1903
Size: 34 3323 34 3678
Fragments arranged by size:
3678 3323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4961 6171 4961
Size: 1210 5859
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7069
BfrBI ATG'CAT
Cuts at: 3482 3482
Size: 7069
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5680 136
Size: 107 122 71 631 605 4008 1525
Fragments arranged by size:
4008 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7064 7064
Size: 7069
BlpI GC'TnA_GC
Cuts at: 4486 4486
Size: 7069
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2676 2774 4291 5130 6286 1790
Size: 319 567 98 1517 839 1156 2573
Fragments arranged by size:
2573 1517 1156 839 567 319 98
BmgBI CAC'GTC
Cuts at: 1318 2058 3576 3630 1318
Size: 740 1518 54 4757
Fragments arranged by size:
4757 1518 740 54
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3047 3107 4800
Size: 416 53 83 399 24 1630 60 1693
Cuts at: 4800 5758 442
Size: 958 1753
Fragments arranged by size:
1753 1693 1630 958 416 399 83 60
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7069
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2970 4251 2970
Size: 1281 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2561 3695 5729 1239
Size: 1322 1134 2034 2579
Fragments arranged by size:
2579 2034 1322 1134
Bpu10I CC'TnA_GC
Cuts at: 3746 4101 4200 3746
Size: 355 99 6615
Fragments arranged by size:
6615 355 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 2942 2984 5194 6059 6357 2176
Size: 766 42 2210 865 298 2888
Fragments arranged by size:
2888 2210 865 766 298 42
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2138 3836 5732 882
Size: 617 639 1698 1896 2219
Fragments arranged by size:
2219 1896 1698 639 617
BsaAI yAC'GTr
Cuts at: 493 1204 4860 493
Size: 711 3656 2702
Fragments arranged by size:
3656 2702 711
BsaBI GATnn'nnATC
Cuts at: 7063 7063
Size: 7069
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2571 4876 5258 5893
Size: 53 83 186 936 1038 2305 382 635
Cuts at: 5893 275
Size: 1451
Fragments arranged by size:
2305 1451 1038 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5502 5900 6174 6321 6732 5502
Size: 398 274 147 411 5839
Fragments arranged by size:
5839 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3037 3067 1178
Size: 30 165 30 1634 30 5180
Fragments arranged by size:
5180 1634 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2141 2751 2951 2959 3572 4145 1186
Size: 952 3 610 200 8 613 573 4110
Fragments arranged by size:
4110 952 613 610 573 200 8 3
BseYI C'CCAG_C
Cuts at: 2675 2929 3179 4354 6272 6768 2675
Size: 254 250 1175 1918 496 2976
Fragments arranged by size:
2976 1918 1175 496 254 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 4251 1163
Size: 573 324 2191 3981
Fragments arranged by size:
3981 2191 573 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4519 5280 6308 1540
Size: 275 186 2518 761 1028 2301
Fragments arranged by size:
2518 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2951 3774 4102 5130 5215 6286 2951
Size: 823 328 1028 85 1071 3734
Fragments arranged by size:
3734 1071 1028 823 328 85
BsmI GAATG_Cn'
Cuts at: 4580 4673 4580
Size: 93 6976
BsmBI CGTCTCn'nnnn_
Cuts at: 3117 3620 3117
Size: 503 6566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2676 2774 2951 3245 3728 3774
Size: 399 319 567 98 177 294 483 46
Cuts at: 3774 4102 4291 4388 5130 5215 6286 1391
Size: 328 189 97 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 483 399 328 319
294 189 177 98 97 85 46
BspEI T'CCGG_A
Cuts at: 6732 6732
Size: 7069
BspHI T'CATG_A
Cuts at: 4958 6688 4958
Size: 1730 5339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3080 4424 844
Size: 2236 1344 3489
Fragments arranged by size:
3489 2236 1344
BsrBI CCG'CTC
Cuts at: 1394 1823 4956 1394
Size: 429 3133 3507
Fragments arranged by size:
3507 3133 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5564 5738 65
Size: 1183 4316 174 1396
Fragments arranged by size:
4316 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 5713 5900 1700
Size: 110 274 3629 187 2869
Fragments arranged by size:
3629 2869 274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7069
BssSI C'ACGA_G
Cuts at: 3120 5129 6141 3120
Size: 2009 1012 4048
Fragments arranged by size:
4048 2009 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7069
BstBI TT'CG_AA
Cuts at: 4207 5873 4207
Size: 1666 5403
BstEII G'GTnAC_C
Cuts at: 1184 2611 1184
Size: 1427 5642
BstYI r'GATC_y
Cuts at: 1972 2025 3157 3283 3738 4399 4766 5151 5168
Size: 53 1132 126 455 661 367 385 17
Cuts at: 5168 6620 6706 6718 7064 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1132 661 455 385 367 346
126 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2717 2717
Size: 7069
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 2481 3279 3966 513
Size: 552 939 14 463 798 687 3616
Fragments arranged by size:
3616 939 798 687 552 463 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2603 487
Size: 683 56 1377 4953
Fragments arranged by size:
4953 1377 683 56
BtsI GCAGTG_nn'
Cuts at: 2048 3322 3510 4585 5379 5399 2048
Size: 1274 188 1075 794 20 3718
Fragments arranged by size:
3718 1274 1075 794 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3646 3681 529
Size: 35 3082 35 3917
Fragments arranged by size:
3917 3082 35 35
DraI TTT'AAA
Cuts at: 3292 4373 3292
Size: 1081 5988
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6076 809
Size: 507 4760 1802
Fragments arranged by size:
4760 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3541 4516 5405
Size: 54 1425 50 275 18 1711 975 889
Cuts at: 5405 7034 8
Size: 1629 43
Fragments arranged by size:
1711 1629 1425 975 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4516 1537
Size: 275 2704 4090
Fragments arranged by size:
4090 2704 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 3238 3783 3834 3941 4031 4161 4185
Size: 1452 433 545 51 107 90 130 24
Cuts at: 4185 4999 1353
Size: 814 3423
Fragments arranged by size:
3423 1452 814 545 433 130 107 90
51 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5656 6030 6176 387
Size: 1064 138 4067 374 146 1280
Fragments arranged by size:
4067 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2949 2949
Size: 7069
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2217 2340 2561 2785 2829 3695
Size: 393 564 21 123 221 224 44 866
Cuts at: 3695 4012 5132 5729 6516 6784 1239
Size: 317 1120 597 787 268 1524
Fragments arranged by size:
1524 1120 866 787 597 564 393 317
268 224 221 123 44 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2838 2838
Size: 7069
EcoO109I rG'GnC_Cy
Cuts at: 2261 3694 3982 4011 4147 4287 4288 2261
Size: 1433 288 29 136 140 1 5042
Fragments arranged by size:
5042 1433 288 140 136 29 1
EcoRI G'AATT_C
Cuts at: 4378 4756 4378
Size: 378 6691
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 7069
FspI TGC'GCA
Cuts at: 5575 5575
Size: 7069
HaeII r_GCGC'y
Cuts at: 1678 6216 1678
Size: 4538 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2378 2410 3886
Size: 32 135 32 563 32 211 32 1476
Cuts at: 3886 3918 3983 4015 5710 5742 5784 5816 1373
Size: 32 65 32 1695 32 42 32 2626
Fragments arranged by size:
2626 1695 1476 563 211 135 65 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4413 4659 669
Size: 988 281 2475 246 3079
Fragments arranged by size:
3079 2475 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2960 3023 748
Size: 2212 63 4794
Fragments arranged by size:
4794 2212 63
HpaI GTT'AAC
Cuts at: 4659 4659
Size: 7069
KpnI G_GTAC'C
Cuts at: 4394 4394
Size: 7069
MfeI C'AATT_G
Cuts at: 2730 4668 2730
Size: 1938 5131
MluI A'CGCG_T
Cuts at: 6945 6945
Size: 7069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2796 6183 6367 1305
Size: 1491 3387 184 2007
Fragments arranged by size:
3387 2007 1491 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3543 10
Size: 54 1425 343 1711 3536
Fragments arranged by size:
3536 1711 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 3348 5027 5386 5545 518
Size: 648 1357 825 1679 359 159 2042
Fragments arranged by size:
2042 1679 1357 825 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3596 3767 4485 5162 6310 6555 1606
Size: 1990 171 718 677 1148 245 2120
Fragments arranged by size:
2120 1990 1148 718 677 245 171
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 7069
NcoI C'CATG_G
Cuts at: 513 1065 2004 3279 3966 513
Size: 552 939 1275 687 3616
Fragments arranged by size:
3616 1275 939 687 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7069
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 7069
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7069
NotI GC'GGCC_GC
Cuts at: 4516 4516
Size: 7069
NsiI A_TGCA'T
Cuts at: 3484 3484
Size: 7069
NspI r_CATG'y
Cuts at: 1132 1229 3486 4427 5972 1132
Size: 97 2257 941 1545 2229
Fragments arranged by size:
2257 2229 1545 941 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7069
PciI A'CATG_T
Cuts at: 5968 5968
Size: 7069
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3640 1108
Size: 2532 4537
PfoI T'CCnGG_A
Cuts at: 2577 2577
Size: 7069
PmeI GTTT'AAAC
Cuts at: 4373 4373
Size: 7069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5138 5170 6651 6683 5138
Size: 32 1481 32 5524
Fragments arranged by size:
5524 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2261 4011 2261
Size: 1750 5319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7069
PsiI TTA'TAA
Cuts at: 4639 4639
Size: 7069
PspOMI G'GGCC_C
Cuts at: 2105 4287 2105
Size: 2182 4887
PspXI vC'TCGA_Gb
Cuts at: 2618 2618
Size: 7069
PstI C_TGCA'G
Cuts at: 830 2536 3216 3688 4296 4421 830
Size: 1706 680 472 608 125 3478
Fragments arranged by size:
3478 1706 680 608 472 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7069
PvuII CAG'CTG
Cuts at: 3596 3767 3596
Size: 171 6898
SacI G_AGCT'C
Cuts at: 2951 2951
Size: 7069
SalI G'TCGA_C
Cuts at: 1936 4411 1936
Size: 2475 4594
SapI GCTCTTCn'nnn_
Cuts at: 3941 3941
Size: 7069
SbfI CC_TGCA'GG
Cuts at: 4421 4421
Size: 7069
ScaI AGT'ACT
Cuts at: 5317 7029 5317
Size: 1712 5357
SfcI C'TryA_G
Cuts at: 826 1045 2532 3105 3212 3684 4292 4417 5552
Size: 219 1487 573 107 472 608 125 1135
Cuts at: 5552 6233 6424 826
Size: 681 191 1471
Fragments arranged by size:
1487 1471 1135 681 608 573 472 219
191 125 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
SgrAI Cr'CCGG_yG
Cuts at: 1700 2084 1700
Size: 384 6685
SmaI CCC'GGG
Cuts at: 1872 4396 1872
Size: 2524 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2155 2618 2957 2963 5173 6074
Size: 197 926 212 463 339 6 2210 901
Cuts at: 6074 6336 820
Size: 262 1553
Fragments arranged by size:
2210 1553 926 901 463 339 262 212
197 6
SnaBI TAC'GTA
Cuts at: 493 4860 493
Size: 4367 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7069
SphI G_CATG'C
Cuts at: 1132 4427 1132
Size: 3295 3774
SspI AAT'ATT
Cuts at: 5 52 4993 5
Size: 47 4941 2081
Fragments arranged by size:
4941 2081 47
StuI AGG'CCT
Cuts at: 1517 2624 1517
Size: 1107 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2784 3017 3279 3966 4508 513
Size: 552 939 780 233 262 687 542 3074
Fragments arranged by size:
3074 939 780 687 552 542 262 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3382 5096 5113 5266 5451 5896 325
Size: 1255 1802 1714 17 153 185 445 1498
Fragments arranged by size:
1802 1714 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5315 7027 96
Size: 274 80 33 51 1443 3338 1712 138
Fragments arranged by size:
3338 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3037 3069 7029 7061 3037
Size: 32 3960 32 3045
Fragments arranged by size:
3960 3045 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2059 1316
Size: 743 6326
XbaI T'CTAG_A
Cuts at: 4405 4405
Size: 7069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2761 2761
Size: 7069
XhoI C'TCGA_G
Cuts at: 1943 2618 1943
Size: 675 6394
XmaI C'CCGG_G
Cuts at: 1870 4394 1870
Size: 2524 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 5198 3950
Size: 1248 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4877 276
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstXI BstZ17I DraIII FalI FseI FspAI KasI NarI
NruI PacI PmlI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI